RESUMO
The purpose of this study was to elucidate the possible immunomodulating effect of hyperbaric oxygenation (HBO) on the immune status of patients with various ophthalmopathies and developing the graft-versus-host reaction after perforating keratoplasty. Surface phenotype of the peripheral blood lymphocytes was studied by two-color flow cytofluorometry in donors and patients with the rejection syndrome after transplantation of the cornea before and after a course of oxygen therapy. HBO exerted no immunodepressive effect, as judged from changes in the subpopulation composition (CD3+, CD4+, CD8+, CD16+, and CD56+) and expression of activation markers (CD25 and HLA-DR), in both patients and donors. The most probable explanation is that HBO effect on the immune system is indirect. On the other hand, from a clinical viewpoint the effect of HBO is undoubtedly positive, and hence, HBO exposure may be recommended for clinical application in transplantations of the cornea.
Assuntos
Reação Enxerto-Hospedeiro , Oxigenoterapia Hiperbárica , Ceratoplastia Penetrante , Fenótipo , Subpopulações de Linfócitos T/imunologia , Adulto , Idoso , Feminino , Citometria de Fluxo , Humanos , Recém-Nascido , Masculino , Pessoa de Meia-Idade , Doadores de TecidosRESUMO
It is shown in experiment on the rabbits that the autologous supernatants of the culture of stimulating lymphocytes and the fractions of lymphokines with mol. weight 40000-20000 and 60000-70000 considerably reduced the periods of wound healing.