Assuntos
Fibrinolíticos/farmacologia , Tuftsina/farmacologia , Animais , Coagulação Sanguínea/efeitos dos fármacos , Bovinos , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Fibrinólise/efeitos dos fármacos , Técnicas In Vitro , Masculino , Ratos , Tromboplastina , Trombose/induzido quimicamente , Trombose/prevenção & controleRESUMO
Complexes of low-molecular heparin with acetylsalicilic acid was formed in vitro when the weight ratio of components was 1:1, 1:5 and 5:1, respectively. All the complexes possessed fibrinolytic and anticoagulating activities. The complex possessed the highest activity when the ratio of heparin to acetylsalicilic acid was 5:1. This complex at a dose 1 mg/200 g of rat weight had the largest thrombolytic effect on the experimental fresh thrombus.
Assuntos
Aspirina/uso terapêutico , Fibrinolíticos/uso terapêutico , Heparina de Baixo Peso Molecular/uso terapêutico , Animais , Anticoagulantes/farmacologia , Anticoagulantes/uso terapêutico , Aspirina/farmacologia , Testes de Coagulação Sanguínea , Combinação de Medicamentos , Avaliação Pré-Clínica de Medicamentos , Fibrinolíticos/farmacologia , Heparina de Baixo Peso Molecular/farmacologia , Veias Jugulares , Masculino , Ratos , Trombose/sangue , Trombose/tratamento farmacológicoRESUMO
In the present work, the nature of an anticoagulant from Philipendula ulmaria was studied. A method for purification of this anticoagulant was developed. Using diverse methods it was shown that the molecular weight, data on element (sulphur, nitrogen, and hydrogen) content, spectral characteristics in the infrared region of the spectrum, and electrophoretic properties of the product indicate its similarity to heparin of animal origin.
Assuntos
Heparina/isolamento & purificação , Plantas Medicinais , Animais , Cromatografia em Gel , Eletroforese em Gel de Poliacrilamida , Heparina/análise , Heparina/farmacologia , Peso Molecular , Tempo de Tromboplastina Parcial , Ratos , Espectrometria de Massas de Bombardeamento Rápido de Átomos , Espectrofotometria Infravermelho , Tempo de TrombinaRESUMO
The flowers of Filipendula ulmaria were found to contain heparin bound to the plant proteins in the form of a complex. This complex enhances the anticoagulant and fibrinolytic properties of the nonenzymatic nature at its administration to animals both intramuscularly and intravenously. The neutralizing effect of protaminesulphate on the anticoagulant activity of the plant heparin was shown. The identity of the action on the hemostasis system of heparin of animal and plant origin was found.
Assuntos
Heparina/análise , Plantas Medicinais/análise , Animais , Coagulação Sanguínea/efeitos dos fármacos , Fibrinólise/efeitos dos fármacos , Heparina/isolamento & purificação , Heparina/farmacologia , Masculino , Proteínas de Plantas/análise , Protaminas/farmacologia , Ligação Proteica , Ratos , Fatores de TempoRESUMO
It has been determined that the thromboplastic agents from the inflorescence of the birch Betula pendula Roth, blossoms of the willow Salix daphnoides Vill., seeds of the pea Pisum sativum L. provoke protective reaction of the animal's anticoagulation system, though weaker expressed than the reaction of thromboplastin from brain. The mechanisms of action of thromboplastic agents of plant origin is similar to the mechanism of action of tissue thromboplastin.
Assuntos
Hemostasia/efeitos dos fármacos , Extratos Vegetais/farmacologia , Plantas Medicinais , Tromboplastina/farmacologia , Animais , Fabaceae , Fibrinólise/efeitos dos fármacos , Masculino , Ratos , Sementes , Fatores de Tempo , ÁrvoresRESUMO
Hemostatic properties of extracts isolated from the birch floscules and willow blossoms have been revealed. An agent of thromboplastic nature which is identical to thromboplasin of the rat brain according to its biochemical composition and procoagulative action has been found in these extracts. In contrast to brain thromboplastin thromboplastic activity of plant extracts does not possess the species specificity. After intravenous injection of the plant extracts a reaction similar to defence reaction of the anticoagulation system in response to thromboplastin infusion of the rat brain develops in rats.
Assuntos
Coagulantes , Extratos Vegetais/farmacologia , Proteínas de Plantas/farmacologia , Tromboplastina/farmacologia , Árvores , Animais , Humanos , Técnicas In Vitro , Fosfolipídeos/análise , Extratos Vegetais/análise , Proteínas de Plantas/análise , Ratos , Tromboplastina/análiseRESUMO
It is shown that the damage of sarcoma 45 cells at different stages of cell life cycle occurs under the effect of vinblastine treatment against a background of higher activity of the blood anticoagulating system. A decrease in the mitotic activity, mitosis accumulation in prophase and especially in the metaphase as well as essential changes in the interphase cell ultrastructure are detected.
Assuntos
Fibrinólise/efeitos dos fármacos , Sarcoma Experimental/ultraestrutura , Vimblastina/uso terapêutico , Animais , Clorpromazina/uso terapêutico , Avaliação Pré-Clínica de Medicamentos , Sinergismo Farmacológico , Quimioterapia Combinada , Heparina/uso terapêutico , Microscopia Eletrônica , Mitose/efeitos dos fármacos , Ratos , Sarcoma Experimental/tratamento farmacológico , Sarcoma Experimental/fisiopatologia , Ureia/uso terapêuticoRESUMO
It is shown that the epinephrine-heparin-fibrinogen complex, as well as a number of other complex compounds of heparin accomplish the prophylaxis of thrombogenesis at the initial stages of the process, the epinephrine-heparin-fibrinogen complex lysing the thrombi in an average of 48 minutes, while the complexes of fibrinogen-heparin and epinephrine-heparin do this in 2--4 hours. The complex heparin compounds exercise a thrombolytic action only on non-stabilized fresh thrombi with less than 15 minutes from the time of their formation.