Antimicrobial and biocompatibility nature of methanol extract of Lannea coromandelica bark and edible coating film preparation for fruit preservation.

This research was performed to evaluate the antimicrobial activity of methanol extract of Lannea coromandelica bark against fruit damage causing microbes such as fungi: Alternaria sp., Aspergillus sp., Botrytis sp., Cladosporium sp., Fusarium sp., Penicillium sp., Phytophthora sp., and Trichoderma sp. The bacteria: such as Chromobacter sp., Enterobacter sp., Erwinia sp., Flavobacterium sp., Lactobacillus sp., Pseudomonas sp., and Xanthomonas sp. was investigated. Furthermore, their biocompatibility nature was determined through animal (rat) model study and their fruit preserving potential was determined by edible coating preparation with chitosan and other substances. Interestingly, the extract showed dose dependent (1000 µg mL-1) activity against these microbes in the following order: Enterobacter sp. (26.4 ± 1.5) > Chromobacter sp. (25.4 ± 1.6) > Pseudomonas sp. (24.5 ± 1.3) > Flavobacterium sp. (24.3 ± 1.4) > Xanthomonas sp. (23.6 ± 1.6) > Erwinia sp. (23.6 ± 1.6) > Lactobacillus sp. (19.6 ± 1.3). Similarly, the antifungal activity was found as Penicillium sp. (32.6 ± 1.3) > Cladosporium sp. (32.6 ± 1.5) > Alternaria sp. (30.3 ± 1.2) > Aspergillus sp. (29.9 ± 1.8) > Botrytis sp. (29.8 ± 1.2) > Fusarium sp. (28.6 ± 1.5) > Trichoderma sp. (19.8 ± 1.4) > Phytophthora sp. (16.2 ± 1.1). The acute toxicity and histopathological study results revealed that the extract possesses biocompatible in nature. The illumination transmittance and active functional groups involved in interaction among test methanol extract and chitosan investigated by UV-vis and Fourier-transform infrared spectroscopy (FTIR) analyses and found average light transmittance and few vital functional groups accountable for optimistic interaction to creak edible coating. Approximately four (set I-IV) treatment sets were prepared, and it was discovered that all of the coated Citrus maxima fruit quality characteristics including total soluble solids (TSS), weight loss (%), pH of fruit pulp juice, and decay percentage were significantly (p>0.05) better than uncoated fruit.

Assessment of multi-biomedical efficiency of Andrographis paniculata shoot extracts through in-vitro analysis and major compound identification.

The present investigation looked into the various biomedical potentials of Andrographis paniculata shoot extracts. The results showed that the methanol extract (Met-E) of A. paniculata contains more phytochemicals than the acetone and petroleum ether extracts, including alkaloids, saponins, tannins, phenolics, flavonoids, glycosides, terpenoids, phytosterol, steroids, and protein. Accordingly, the Met-E alone showed considerable bactericidal activity (through agar well diffusion method) against the bacterial pathogens namely Shigella dysenteriae, Bacillus cereus, Salmonella typhi, Enterococcus faecalis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphlococcus aureus, E. coli, and B. subtilis. This bactericidal activity was found as dose dependent manner, since at 1000 µg ml concentration, the Met-E showed better antibacterial activity. Similarly, at increased concentration (1000 µg ml) it showed notable antidiabetic (α-amylase inhibition: 74.31% and α-glucosidase inhibition: 72.34%), antioxidant (DPPH: 78.24%), and anti-inflammatory (albumin denaturation inhibition: 79.84% and lipoxigenase inhibition: 69.4%) activities. The phytochemical profiling of Met-E was characterized by UV-visible spectrophotometer (UV-vis), Gas Chromatography-Mass Spectrometry (GC/MS), Fourier transform infrared (FTIR), and High Performance Liquid Chromatography (HPLC) analyses. The results showed the Met-E contain bioactive compounds such as gallic acid, epicatechin, catechin, naringin, vitexin-2-rhamnoside, taxifolin, kaempferol, hesperidin, myricetin, rutin, quercetin, phloretin, and ursolic acid compounds. While most of these substances have been recognised for their pharmacological application perspective, the biological properties of particular substances must be studied in the future using in-vivo strategies.