RESUMO
BACKGROUND: Hematopoetic stem cell transplants (HSCT) are discussed as treatment options for patients with solid tumors. Transplant numbers have changed substantially over the last decade, few controlled studies are available and different opinions prevail. Objective information on current practice is needed. PATIENTS AND METHODS: Data from 27 902 HSCT for solid tumors (2% allogeneic, 98% autologous), collected by the European Group for Blood and Marrow Transplantation (EBMT) activity survey from 1991 to 2002 were used to assess trends, transplant rates and coefficient of variation of transplant rates in Europe. RESULTS: Transplant numbers increased from 536 in 1991 to 4154 in 1997 and decreased to 1913 in 2002. Indications were neuroblastoma (2504 HSCT; 9%), glioma (662 HSCT; 2%), soft tissue sarcoma (1253 HSCT; 4%), germ cell cancer (3291 HSCT; 12%), breast cancer (13 524 HSCT; 48%), Ewing's sarcoma (1896 HSCT; 7%), lung cancer (387 HSCT; 1%), ovarian cancer (845 HSCT; 3%) and other solid tumors (3540 HSCT; 14%). Allogeneic cells were used in <20 cases up to 1997; since then allogeneic HSCT increased to 159 in 2002, mainly for renal cell carcinoma. Low coefficients of variation in transplant rates (<60%) are observed for Ewing's sarcoma (<56.5%), suggesting consensus for this indication. CONCLUSIONS: These data give an overview on current practice of HSCT for solid tumors in Europe. They provide objective information for health-care providers and patient counselling.
Assuntos
Transfusão de Sangue Autóloga/tendências , Transplante de Células-Tronco Hematopoéticas/estatística & dados numéricos , Neoplasias/terapia , Neoplasias da Mama/epidemiologia , Neoplasias da Mama/terapia , Europa (Continente)/epidemiologia , Feminino , Transplante de Células-Tronco Hematopoéticas/tendências , Humanos , Neoplasias Pulmonares/epidemiologia , Neoplasias Pulmonares/terapia , Neoplasias/epidemiologia , Neoplasias Embrionárias de Células Germinativas/epidemiologia , Neoplasias Embrionárias de Células Germinativas/terapia , Neuroblastoma/epidemiologia , Neuroblastoma/terapia , Neoplasias Ovarianas/epidemiologia , Neoplasias Ovarianas/terapia , Sarcoma de Ewing/epidemiologia , Sarcoma de Ewing/terapia , Neoplasias de Tecidos Moles/epidemiologia , Neoplasias de Tecidos Moles/terapia , Fatores de TempoAssuntos
Glutationa Peroxidase , Peroxidases , Sequência de Aminoácidos , Animais , Sítios de Ligação , Evolução Biológica , Catálise , Bovinos , Análise de Fourier , Glutationa Peroxidase/sangue , Substâncias Macromoleculares , Modelos Moleculares , Peroxidases/sangue , Conformação Proteica , SelênioRESUMO
Glutathione peroxidase showed an X-ray photoelectron spectroscopy signal of the Se 3d (3/2, 5/2) electrons at 54.4 eV. After the addition of the acceptor substrate H2O2, a marked shift of this signal to a value of 58.0 eV was observed. Upon subsequent treatment with the donor substrate glutathione, this chemical shift was reversed and the original signal was obtained. These data demonstrate that the enzyme-bound selenium moiety participates in the catalytic process. From the chemical shift obtained it is concluded that the enzyme shuttles between a selenol or selenol derivative in its reduced form and a seleninyl or selenonyl compound in its oxidized form.