RESUMO
OBJECTIVE: This study aimed to determine the effectiveness of Centella asiatica leaf extract to TNF-α levels. METHODS: There were four treatment groups (each group consisted of five rats). Group I was given 0.5% Na CMC, group II was given Cefadroxil 45mg/kg WB, Group III C. asiatica leaf extract 100mg/kg BW, and group IV combination Cefadroxil and Centella leaf extract. Each group was given treatment twice for 12h a day for five days. RESULTS: TNF-α levels between groups did not significantly affect day three and differed significantly after day 6. On day 3, the control group had a higher TNF-α level of 25.13pg/ml than the group given antibiotics and C. asiatica. While when compared to the group given only C. asiatica leaf extract, the control group was more height of 17.1pg/ml. On the 6th day, this condition was changed. The most significant difference was found in the group given C. asiatica, in which the control group had higher levels of TNF-α 72.34pg/ml than the group receiving C. asiatica. Then, the control group is higher than 66.46pg/ml than those given antibiotics and C. asiatica. CONCLUSION: C. asiatica leaf extract effectively reduces TNF both given alone and given along with antibiotics. It is potential to be explored into alternative and complementary treatments in mastitis cases with human trials.