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1.
Food Chem ; 206: 234-8, 2016 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-27041321

RESUMO

Enriching oils, such as olive oil, could be one solution to tackle the worldwide epidemic of vitamin D deficiency and to better fit with omega 3 (DHA) recommendations. However, data regarding the interactions occurring at the intestinal level between vitamin D and phenols from olive oil are scarce. We first determined the effect of polyphenols from a virgin olive oil, and a virgin olive oil enriched with DHA, on vitamin D absorption in rats. We then investigated the effects of 3 main olive oil phenols (oleuropein, hydroxytyrosol and pinoresinol) on vitamin D uptake by Caco-2 cells. The presence of polyphenols in the olive oil supplemented with DHA inhibited vitamin D postprandial response in rats (-25%, p<0.05). Similar results were obtained with a mix of the 3 polyphenols delivered to Caco-2 cells. However, this inhibitory effect was due to the presence of pinoresinol only. As the pinoresinol content can highly vary between olive oils, the present results should be taken into account to formulate an appropriate oil product enriched in vitamin D.


Assuntos
Furanos/análise , Absorção Intestinal/efeitos dos fármacos , Lignanas/análise , Azeite de Oliva/química , Vitamina D/farmacocinética , Animais , Células CACO-2 , Ácidos Docosa-Hexaenoicos/análise , Feminino , Humanos , Glucosídeos Iridoides , Iridoides/análise , Álcool Feniletílico/análogos & derivados , Álcool Feniletílico/análise , Polifenóis/análise , Ratos , Ratos Wistar , Vitamina D/antagonistas & inibidores
2.
Nutr Res ; 36(2): 161-73, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26574736

RESUMO

Dietary supplementation with dried plum (DP) has been shown to protect against and reverse established osteopenia in ovariectomized rodents. Based on in vitro studies, we hypothesized that DP polyphenols may be responsible for that bone-sparing effect. This study was designed to (1) analyze whether the main phenolic acids of DP control preosteoblast proliferation and activity in vitro; (2) determine if the polyphenolic content of DP or DP juice concentrate is the main component improving bone health in vivo; and (3) analyze whether DP metabolites directly modulate preosteoblast physiology ex vivo. In vitro, we found that neochlorogenic, chlorogenic, and caffeic acids induce the proliferation and repress the alkaline phosphatase activity of primary preosteoblasts in a dose-dependent manner. In vivo, low-chlorogenic acid Agen prunes (AP) enriched with a high-fiber diet and low-chlorogenic acid AP juice concentrate prevented the decrease of total femoral bone mineral density induced by estrogen deficiency in 5-month-old female rats and positively restored the variations of the bone markers osteocalcin and deoxypyridinoline. Ex vivo, we demonstrated that serum from rats fed with low-chlorogenic acid AP enriched with a high-fiber diet showed repressed proliferation and stimulated alkaline phosphatase activity of primary preosteoblasts. Overall, the beneficial action of AP on bone health was not dependent on its polyphenolic content.


Assuntos
Conservadores da Densidade Óssea/uso terapêutico , Ácidos Cafeicos/uso terapêutico , Ácido Clorogênico/análogos & derivados , Suplementos Nutricionais , Modelos Animais de Doenças , Osteoporose Pós-Menopausa/prevenção & controle , Prunus domestica/química , Ácido Quínico/análogos & derivados , Animais , Biomarcadores/sangue , Biomarcadores/urina , Densidade Óssea , Ácidos Cafeicos/análise , Proliferação de Células , Células Cultivadas , Ácido Clorogênico/análise , Ácido Clorogênico/uso terapêutico , Suplementos Nutricionais/análise , Feminino , Frutas/química , Sucos de Frutas e Vegetais/análise , Humanos , Osteoblastos/citologia , Osteoblastos/metabolismo , Osteoblastos/patologia , Osteogênese , Osteoporose Pós-Menopausa/sangue , Osteoporose Pós-Menopausa/urina , Ácido Quínico/análise , Ácido Quínico/uso terapêutico , Distribuição Aleatória , Ratos Wistar
3.
Nutrients ; 7(11): 9265-84, 2015 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-26569295

RESUMO

The nutritional benefits of pomegranate have attracted great scientific interest. The pomegranate, including the pomegranate peel, has been used worldwide for many years as a fruit with medicinal activity, mostly antioxidant properties. Among chronic diseases, osteoporosis, which is associated with bone remodelling impairment leading to progressive bone loss, could eventually benefit from antioxidant compounds because of the involvement of oxidative stress in the pathogenesis of osteopenia. In this study, with in vivo and ex vivo experiments, we investigated whether the consumption of pomegranate peel extract (PGPE) could limit the process of osteopenia. We demonstrated that in ovariectomized (OVX) C57BL/6J mice, PGPE consumption was able to significantly prevent the decrease in bone mineral density (-31.9%; p < 0.001 vs. OVX mice) and bone microarchitecture impairment. Moreover, the exposure of RAW264.7 cells to serum harvested from mice that had been given a PGPE-enriched diet elicited reduced osteoclast differentiation and bone resorption, as shown by the inhibition of the major osteoclast markers. In addition, PGPE appeared to substantially stimulate osteoblastic MC3T3-E1 alkaline phosphatase (ALP) activity at day 7, mineralization at day 21 and the transcription level of osteogenic markers. PGPE may be effective in preventing the bone loss associated with ovariectomy in mice, and offers a promising alternative for the nutritional management of this disease.


Assuntos
Densidade Óssea/efeitos dos fármacos , Lythraceae/química , Osteoblastos/efeitos dos fármacos , Osteoporose/prevenção & controle , Extratos Vegetais/farmacologia , Células 3T3 , Fosfatase Alcalina/metabolismo , Animais , Antioxidantes/farmacologia , Osso e Ossos/efeitos dos fármacos , Osso e Ossos/metabolismo , Diferenciação Celular/efeitos dos fármacos , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Feminino , Frutas/química , Camundongos , Camundongos Endogâmicos C57BL , Osteoblastos/citologia , Ovariectomia , Células RAW 264.7
4.
Eur J Nutr ; 53(5): 1155-64, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24232379

RESUMO

PURPOSE: Recently, nutritional and pharmaceutical benefits of pomegranate (PG) have raised a growing scientific interest. Since PG is endowed with anti-inflammatory and antioxidant activities, we hypothesized that it may have beneficial effects on osteoporosis. METHODS: We used ovariectomized (OVX) mice as a well-described model of postmenopausal osteoporosis to study the influence of PG consumption on bone health. Mice were divided into five groups as following: two control groups sham-operated and ovariectomized (OVX CT) mice fed a standard diet, versus three treated groups OVX mice given a modified diet from the AIN-93G diet, containing 5.7% of PG lyophilized mashed totum (OVX PGt), or 9.6% of PG fresh juice (OVX PGj) or 2.9% of PG lyophilized mashed peel (OVX PGp). RESULTS: As expected, ovariectomy was associated with a decreased femoral bone mineral density (BMD) and impaired bone micro-architecture parameters. Consumption of PGj, PGp, or PGt induced bone-sparing effects in those OVX mice, both on femoral BMD and bone micro-architecture parameters. In addition, PG (whatever the part) up-regulated osteoblast activity and decreased the expression of osteoclast markers, when compared to what was observed in OVX CT animals. Consistent with the data related to bone parameters, PG consumption elicited a lower expression of pro-inflammatory makers and of enzymes involved in ROS generation, whereas the expression of anti-inflammatory markers and anti-oxidant actors was enhanced. CONCLUSION: These results indicate that all PG parts are effective in preventing the development of bone loss induced by ovariectomy in mice. Such an effect could be partially explained by an improved inflammatory and oxidative status.


Assuntos
Osso e Ossos/efeitos dos fármacos , Inflamação/tratamento farmacológico , Lythraceae/química , Osteoporose/prevenção & controle , Estresse Oxidativo/efeitos dos fármacos , Preparações de Plantas/farmacologia , Animais , Anti-Inflamatórios/farmacologia , Antioxidantes/farmacologia , Densidade Óssea/efeitos dos fármacos , Osso e Ossos/metabolismo , Modelos Animais de Doenças , Feminino , Camundongos , Camundongos Endogâmicos C57BL , Ovariectomia , Fitoterapia
5.
PLoS One ; 9(12): e115817, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25551374

RESUMO

As the Mediterranean diet (and particularly olive oil) has been associated with bone health, we investigated the impact of extra virgin oil as a source of polyphenols on bone metabolism. In that purpose sham-operated (SH) or ovariectomized (OVX) mice were subjected to refined or virgin olive oil. Two supplementary OVX groups were given either refined or virgin olive oil fortified with vitamin D3, to assess the possible synergistic effects with another liposoluble nutrient. After 30 days of exposure, bone mineral density and gene expression were evaluated. Consistent with previous data, ovariectomy was associated with increased bone turnover and led to impaired bone mass and micro-architecture. The expression of oxidative stress markers were enhanced as well. Virgin olive oil fortified with vitamin D3 prevented such changes in terms of both bone remodeling and bone mineral density. The expression of inflammation and oxidative stress mRNA was also lower in this group. Overall, our data suggest a protective impact of virgin olive oil as a source of polyphenols in addition to vitamin D3 on bone metabolism through improvement of oxidative stress and inflammation.


Assuntos
Desmineralização Patológica Óssea/prevenção & controle , Densidade Óssea/efeitos dos fármacos , Óleos de Plantas/uso terapêutico , Vitamina D/uso terapêutico , Animais , Composição Corporal/efeitos dos fármacos , Conservadores da Densidade Óssea/uso terapêutico , Osso e Ossos/metabolismo , Osso e Ossos/patologia , Dieta Mediterrânea , Gorduras Insaturadas na Dieta/uso terapêutico , Sinergismo Farmacológico , Estrogênios/deficiência , Feminino , Camundongos , Camundongos Endogâmicos C57BL , Azeite de Oliva , Ovariectomia , Estresse Oxidativo/efeitos dos fármacos , Polifenóis/metabolismo
6.
J Nutr Biochem ; 24(11): 1840-8, 2013 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-23953990

RESUMO

In the current context of longer life expectancy, the prevalence of osteoporosis is increasingly important. This is why development of new strategies of prevention is highly suitable. Pomegranate seed oil (PSO) and its major component, punicic acid (a conjugated linolenic acid), have potent anti-inflammatory and anti-oxidative properties both in vitro and in vivo, two processes strongly involved in osteoporosis establishment. In this study, we demonstrated that PSO consumption (5% of the diet) improved significantly bone mineral density (240.24±11.85 vs. 203.04±34.19 mg/cm(3)) and prevented trabecular microarchitecture impairment in ovariectomized (OVX) mice C57BL/6J, compared to OVX control animals. Those findings are associated with transcriptional changes in bone tissue, suggesting involvement of both osteoclastogenesis inhibition and osteoblastogenesis improvement. In addition, thanks to an ex vivo experiment, we provided evidence that serum from mice fed PSO (5% by gavage) had the ability to significantly down-regulate the expression of specific osteoclast differentiation markers and RANK-RANKL downstream signaling targets in osteoclast-like cells (RAW264.7) (RANK: negative 0.49-fold vs. control conditions). Moreover, in osteoblast-like cells (MC3T3-E1), it elicited significant increase in alkaline phosphatase activity (+159% at day 7), matrix mineralization (+271% on day 21) and transcriptional levels of major osteoblast lineage markers involving the Wnt/ß-catenin signaling pathways. Our data also reveal that PSO inhibited pro-inflammatory factors expression while stimulating anti-inflammatory ones. These results demonstrate that PSO is highly relevant regarding osteoporosis. Indeed, it offers promising alternatives in the design of new strategies in nutritional management of age-related bone complications.


Assuntos
Lythraceae/química , Osteoporose/prevenção & controle , Óleos de Plantas/uso terapêutico , Sementes/química , Animais , Densidade Óssea , Linhagem Celular , Proliferação de Células , Modelos Animais de Doenças , Feminino , Interleucina-1/biossíntese , Interleucina-6/biossíntese , Ácidos Linolênicos/uso terapêutico , Camundongos , Osteoblastos/efeitos dos fármacos , Osteoblastos/fisiologia , Osteoclastos/efeitos dos fármacos , Osteoclastos/fisiologia , Ovariectomia , Receptores de Interleucina-6/antagonistas & inibidores
7.
Bone ; 50(2): 553-61, 2012 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-21664309

RESUMO

Fats are prevalent in western diets; they have known deleterious effects on muscle insulin resistance and may contribute to bone loss. However, relationships between fatty acids and locomotor system dysfunctions in elderly population remain controversial. The aim of this study was to analyze the impact of fatty acid quality on the age related evolution of the locomotor system and to understand which aging mechanisms are involved. In order to analyze age related complications, the SAMP8 mouse strain was chosen as a progeria model as compared to the SAMR1 control strain. Then, two months old mice were divided in different groups and subjected to the following diets : (1) standard "growth" diet - (2) "sunflower" diet (high ω6/ω3 ratio) - (3) "borage" diet (high γ-linolenic acid) - (4) "fish" diet (high in long chain ω3). Mice were fed ad libitum through the whole protocol. At 12 months old, the mice were sacrificed and tissues were harvested for bone studies, fat and muscle mass measures, inflammation parameters and bone cell marker expression. We demonstrated for the first time that borage and fish diets restored inflammation and bone parameters using an original model of senile osteoporosis that mimics clinical features of aging in humans. Therefore, our study strongly encourages nutritional approaches as relevant and promising strategies for preventing aged-related locomotor dysfunctions.


Assuntos
Osso e Ossos/patologia , Borago/química , Suplementos Nutricionais , Óleos de Peixe/uso terapêutico , Inflamação/tratamento farmacológico , Osteoporose/tratamento farmacológico , Óleos de Plantas/uso terapêutico , Adiposidade/efeitos dos fármacos , Animais , Biomarcadores/metabolismo , Remodelação Óssea/efeitos dos fármacos , Osso e Ossos/efeitos dos fármacos , Calcificação Fisiológica/efeitos dos fármacos , Gorduras na Dieta/análise , Modelos Animais de Doenças , Feminino , Óleos de Peixe/farmacologia , Saúde , Helianthus , Inflamação/complicações , Inflamação/fisiopatologia , Camundongos , Camundongos Mutantes , Tamanho do Órgão/efeitos dos fármacos , Osteoporose/patologia , Osteoporose/fisiopatologia , Óleos de Plantas/farmacologia
8.
Pediatr Res ; 66(5): 513-8, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19668107

RESUMO

Peak bone mass is a major determinant of osteoporosis pathogenesis during aging. Respective influences of energy and protein supplies on skeletal growth remains unclear. We investigated the effect of a 5-mo dietary restriction on bone status in young rats randomized into six groups (n = 10 per group). Control animals were fed a diet containing a normal (13%) (C-NP) or a high-protein content (26%) (C-HP). The other groups received a 40% protein energy-restricted diet (PER-NP and PER-HP) or a 40% energy-restricted diet (ER-NP and ER-HP). High-protein intake did not modulate bone acquisition, although a metabolic acidosis was induced and calcium retention impaired. PER and ER diets were associated with a decrease in femoral bone mineral density. The compensation for protein intake in energy-restricted conditions induced a bone sparing effect. Plasma osteocalcin (OC) and urinary deoxypyridinoline (DPD) assays revealed a decreased OC/DPD ratio in restricted rats compared with C animals, which was far more reduced in PER than in ER groups. Circulating IGF-1 levels were lowered by dietary restrictions. In conclusion, both energy and protein deficiencies may contribute to impairment in peak bone mass acquisition, which may affect skeleton strength and potentially render individuals more susceptible to osteoporosis.


Assuntos
Osso e Ossos/patologia , Suplementos Nutricionais , Aminoácidos/urina , Ração Animal , Animais , Fenômenos Biomecânicos , Densidade Óssea , Fêmur/anatomia & histologia , Fator de Crescimento Insulin-Like I/metabolismo , Íons , Masculino , Osteocalcina/sangue , Osteoporose/patologia , Ratos , Ratos Wistar
9.
Br J Nutr ; 102(7): 976-84, 2009 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-19393110

RESUMO

Hesperidin (Hp), a citrus flavonoid predominantly found in oranges, shows bone-sparing effects in ovariectomised (OVX) animals. In human subjects, the bioavailability of Hp can be improved by the removal of the rhamnose group to yield hesperetin-7-glucoside (H-7-glc). The aim of the present work was to test whether H-7-glc was more bioavailable and therefore more effective than Hp in the prevention of bone loss in the OVX rat. Adult 6-month-old female Wistar rats were sham operated or OVX, then pair fed for 90 d a casein-based diet supplemented or not with freeze-dried orange juice enriched with Hp or H-7-glc at two dose equivalents of the hesperetin aglycone (0.25 and 0.5 %). In the rats fed 0.5 %, a reduction in OVX-induced bone loss was observed regarding total bone mineral density (BMD):+7.0 % in OVX rats treated with Hp (HpOVX) and +6.6 % in OVX rats treated with H-7-glc (H-7-glcOVX) v. OVX controls (P < 0.05). In the rats fed 0.25 % hesperetin equivalents, the H-7-glcOVX group showed a 6.6 % improvement in total femoral BMD v. the OVX controls (P < 0.05), whereas the Hp diet had no effect at this dose. The BMD of rats fed 0.25 % H-7-glc was equal to that of those given 0.5 % Hp, but was not further increased at 0.5 % H-7-glc. Plasma hesperetin levels and relative urinary excretion were significantly enhanced in the H-7-glc v. Hp groups, and the metabolite profile showed the absence of eriodictyol metabolites and increased levels of hesperetin sulphates. Taken together, improved bioavailability of H-7-glc may explain the more efficient bone protection of this compound.


Assuntos
Suplementos Nutricionais , Hesperidina/análogos & derivados , Hesperidina/farmacocinética , Osteoporose/prevenção & controle , Animais , Disponibilidade Biológica , Densidade Óssea/efeitos dos fármacos , Remodelação Óssea/efeitos dos fármacos , Avaliação Pré-Clínica de Medicamentos/métodos , Feminino , Fêmur/patologia , Fêmur/fisiopatologia , Hesperidina/uso terapêutico , Tamanho do Órgão/efeitos dos fármacos , Ovariectomia , Ratos , Ratos Wistar , Útero/patologia , Aumento de Peso/efeitos dos fármacos
10.
J Agric Food Chem ; 56(20): 9417-22, 2008 Oct 22.
Artigo em Inglês | MEDLINE | ID: mdl-18800805

RESUMO

This study was conducted to determine whether the daily consumption for 84 days of tyrosol and hydroxytyrosol, the main olive oil phenolic compounds, and olive oil mill wastewater (OMWW), a byproduct of olive oil production, rich in micronutrients, may improve bone loss in ovariectomized rats (an experimental model of postmenopausal osteoporosis) and in ovariectomized rats with granulomatosis inflammation (a model set up for senile osteoporosis). As expected, an induced chronic inflammation provoked further bone loss at total, metaphyseal, and diaphyseal sites in ovariectomized rats. Tyrosol and hydroxytyrosol prevented this osteopenia by increasing bone formation ( p < 0.05), probably because of their antioxidant properties. The two doses of OMWW extracts had the same protective effect on bone ( p < 0.05), whereas OMWW did not reverse established osteopenia. In conclusion, polyphenol consumption seems to be an interesting way to prevent bone loss.


Assuntos
Conservadores da Densidade Óssea/farmacologia , Inflamação/tratamento farmacológico , Osteoporose/tratamento farmacológico , Ovariectomia/efeitos adversos , Álcool Feniletílico/análogos & derivados , Óleos de Plantas/farmacologia , Animais , Densidade Óssea/efeitos dos fármacos , Modelos Animais de Doenças , Feminino , Flavonoides/farmacologia , Humanos , Azeite de Oliva , Osteoporose/fisiopatologia , Fenóis/farmacologia , Álcool Feniletílico/farmacologia , Óleos de Plantas/química , Polifenóis , Distribuição Aleatória , Ratos , Ratos Wistar
11.
J Nutr ; 138(4): 718-24, 2008 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-18356326

RESUMO

High dietary protein intake generates endogenous acid production, which may adversely affect bone health. Alkaline potassium citrate (Kcit)(2) may contribute to the neutralization of the protein-induced metabolic acidosis. We investigated the impact of 2 levels of protein intake and Kcit supplementation on acid-base metabolism and bone status in rats. Two-month-old Wistar male rats were randomly assigned to 4 groups (n = 30 per group). Two groups received a normal-protein content (13%) (NP) or a high-protein (HP) content diet (26%) for 19 mo. The 2 other groups received identical diets supplemented with Kcit (3.60%) (NPKcit and HPKcit). Rats were pair-fed based on the ad libitum intake of the HP group. At 9, 16, and 21 mo of age, 10 rats of each group were killed. The HP diet induced a metabolic acidosis characterized by hypercalciuria, hypermagnesuria, and hypocitraturia at all ages. Kcit supplementation neutralized this effect, as evidenced by decreased urinary calcium and magnesium excretion by the HPKcit rats. Femoral bone mineral density, biomechanical properties, bone metabolism biomarkers (osteocalcin and deoxypyridinoline), and plasma insulin-like growth factor 1 levels were not affected by the different diets. Nevertheless, at 21 mo of age, calcium retention was reduced in the HP group. This study suggests that lifelong excess of dietary protein results in low-grade metabolic acidosis without affecting the skeleton, which may be protected by an adequate calcium supply.


Assuntos
Equilíbrio Ácido-Base/efeitos dos fármacos , Densidade Óssea/efeitos dos fármacos , Proteínas Alimentares/administração & dosagem , Proteínas Alimentares/farmacologia , Citrato de Potássio/administração & dosagem , Citrato de Potássio/farmacologia , Animais , Ânions/urina , Biomarcadores , Fenômenos Biomecânicos , Peso Corporal , Cálcio/metabolismo , Cátions/urina , Suplementos Nutricionais , Esquema de Medicação , Quimioterapia Combinada , Masculino , Ratos , Ratos Wistar
12.
Clin Nutr ; 25(5): 859-68, 2006 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-16740345

RESUMO

BACKGROUND & AIMS: This study was carried out to assess the dose-dependent bone-sparing effect of oleuropein, an olive oil phenolic compound with anti-inflammatory and anti-oxidative properties, on bone loss induced by talc granulomatosis in oestrogen-deficient rat. METHODS: Among 98 rats, 20 were sham-operated (SH) while the others (78) were ovariectomised (OVX). The SH and 26 OVX rats (controls) were given a standard diet for 100 days. The 52 remaining OVX rats were allocated to 4 groups that received oleuropein at 2.5, 5, 10 or 15 mg/kg body weight per day for 100 days. Three weeks before necropsy, an inflammation was induced by subcutaneous injections of talc in half of the SH and OVX rats and in all oleuropein-treated animals. RESULTS: Castration was associated with a decreased bone mineral density (BMD). In OVX rats, inflammation, characterised by an increase of the spleen weight and plasma fibrinogen levels, exacerbated this bone loss, as shown by values of BMD of the total femur metaphyseal and diaphyseal subregions. The 4 doses of oleuropein reduced bone loss and improved inflammatory biomarkers excepted for 5mg/kg BW. CONCLUSIONS: Every dose of oleuropein elicited protective effects on bone mass in this model of ovariectomy associated with inflammation, probably by modulating inflammatory parameters.


Assuntos
Conservadores da Densidade Óssea/farmacologia , Osteoporose/tratamento farmacológico , Ovariectomia , Piranos/farmacologia , Animais , Anti-Infecciosos/farmacologia , Anti-Infecciosos/uso terapêutico , Biomarcadores/sangue , Densidade Óssea/fisiologia , Conservadores da Densidade Óssea/uso terapêutico , Relação Dose-Resposta a Droga , Feminino , Fibrinogênio/metabolismo , Inflamação/complicações , Glucosídeos Iridoides , Iridoides , Azeite de Oliva , Tamanho do Órgão , Ovariectomia/efeitos adversos , Óleos de Plantas , Piranos/uso terapêutico , Distribuição Aleatória , Ratos , Ratos Wistar , Baço/patologia
13.
Cancer Res ; 63(20): 6607-12, 2003 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-14583453

RESUMO

Phytoestrogens are natural compounds with anticancer, proliferation, differentiation, and chemopreventive effects, for which several mechanisms have been proposed. In the present study, modulation of Brca1 and Brca2 expression by different phytoestrogen-rich diets has been investigated in ovariectomized Wistar rats. Two hundred mammary glands were harvested in three independent experiments. Brca1 and Brca2 mRNAs were quantified by real-time quantitative reverse transcription-PCR, and their proteins by immunohistochemistry. The first experiment compared the influence of different phytoestrogens [flax-seed, isoflavones (IFs), or rutin]. A 10% increase in Brca1 mRNA expression was shown after flax-seed consumption, whereas no variation was noted for Brca2 mRNA, nor for Brca1 and Brca2 proteins. In the second experiment, two soy IFs sources (Novasoy or Soylife) were given at different concentrations to the animals. Only Brca2 mRNA was increased and only at high doses. Finally, the effect of IFs was compared with that of estradiol. An increase in mRNA for both genes was noted after estradiol treatment and with the highest dose of IFs. In conclusion, our results show that IFs, given in the diet at different doses, are able to increase Brca1 and Brca2 mRNA in ovariectomized female Wistar rat. However, no variation in Brca1 or Brca2 protein expression was demonstrated, whatever the experimental conditions were.


Assuntos
Proteína BRCA1/biossíntese , Proteína BRCA2/biossíntese , Isoflavonas/farmacologia , Glândulas Mamárias Animais/metabolismo , Preparações de Plantas/farmacologia , Animais , Proteína BRCA1/genética , Proteína BRCA2/genética , Dieta , Feminino , Regulação da Expressão Gênica/efeitos dos fármacos , Isoflavonas/administração & dosagem , Glândulas Mamárias Animais/efeitos dos fármacos , Glândulas Mamárias Animais/fisiologia , Ovariectomia , Fitoestrógenos , Preparações de Plantas/administração & dosagem , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , Ratos , Ratos Wistar
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