Chronic adverse effects of oil dispersed sediments on growth, hatching, and reproduction of benthic copepods: Indirect exposure for long-term tests.

Laboratory-scale sediment exposure was conducted as a preliminary study to assess the long-term effects of sediment contaminated with crude oil. For this purpose, indirect exposure using a glass filter crucible was tested and compared with direct exposure by observing several parameters (e.g., mortality, growth, reproduction, hatching, and uptake) in the benthic copepod Tigriopus japonicus. In direct exposure, short-term exposure caused significant damages to the eggs of ovigerous females, and there were difficulties in observing small oil droplets. However, indirect exposure did not induce any mortality during a 96-h exposure in adults. A 10-day exposure was also possible in an indirect exposure method and caused a decrease in reproduction and consequently a reduction in the hatching rate. In fact, the water phase collected from indirect exposure indicated significant polycyclic aromatic hydrocarbon (PAH) concentrations, although only a few components were present. The components of PAHs were similar to water-accommodated fractions (WAFs) of crude oil that are associated with the water-soluble part, but the relative portion of high-molecular-weight of PAHs was higher than WAF. In this approach, exposure tests caused reduction in the uptake rate in copepods even in the 24-h exposure. In conclusion, the biological effects of oil droplets from direct exposure were excluded by using a glass filter in indirect exposures, and several parameters could be derived in the long-term exposure. These results indicate that the indirect method could likely assess the chronic effects of oil-contaminated sediments on individual level parameters for deriving the ultimate effects on the population and community.

Acute and chronic toxicity study of the water accommodated fraction (WAF), chemically enhanced WAF (CEWAF) of crude oil and dispersant in the rock pool copepod Tigriopus japonicus.

We determined the toxicity of the water accommodated hydrocarbon fraction (WAF), two chemically enhanced WAFs (CEWAFs; CEWAF-C, Crude oil+Corexit 9500 and CEWAF-H, Crude oil+Hiclean) of crude oil and two dispersants (Corexit 9500 and Hiclean) to the rock pool copepod Tigriopus japonicus. In the acute toxicity test, Corexit 9500 was the most toxic of all the chemicals studied. The nauplius stage of T. japonicus was more susceptible to the toxic chemicals studied than the adult female. The toxicity data using the nauplius stage was then considered as baseline to determine the spiking concentration of chemicals for chronic toxicity tests on the copepod. As the endpoints in the chronic toxicity test, survival, sex ratio, developmental time and fecundity of the copepod were used. All chemicals used in this study resulted in increased toxicity in the F1 generation. The lowest-observed-adverse-effect (LOAE) concentrations of WAF, CEWAF-H, CEWAF-C, Hiclean and Corexit 9500 were observed to be 50%, 10%, 0.1%, 1% and 1%, respectively. The results in present study imply that copepods in marine may be negatively influenced by spilled oil and dispersant.

Two-generation toxicity study on the copepod model species Tigriopus japonicus.

Previous studies on the intertidal copepod Tigriopus japonicus have demonstrated that it is a suitable model species for the assessment of acute toxicities of marine pollutants. In order to standardize T. japonicus for use in environmental risk assessment involving whole life cycle exposure, we tested nine pollutants for their effects on growth and reproduction during a two-generation life cycle exposure test. Nauplii (F 0) were exposed to a range of concentrations of each chemical in a static renewal culture system. Broods of the second generation (F1) were subsequently exposed to the same concentrations for one full life cycle. Of the seven traits (nauplius phase, development time, survival, sex ratio, number of clutch, nauplii per clutch and fecundity), only the length of the nauplius phase and development time showed a greater sensitivity to chemical exposure. Between the two sensitive traits, the period of the nauplius phase was more sensitive than cohort generation time. Biocides significantly increased the maturation period of nauplii as well as copepodids in F 0 generation. In this study, it was demonstrated that T. japonicus could also be used in reproduction and life cycle tests and it provides an opportunity for testing the chronic and subchronic toxic effects of marine pollutants. Further validation and harmonization in a multi-centric study involving other laboratories of the region will strengthen its use as a supplement to existing model species.