Assuntos
Oryza , Oryza/genética , Haploidia , Fosfolipases , Pólen/genética , Proteínas de Plantas/genéticaRESUMO
Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant anthraquinone biosynthesis, we reveal the genome sequence of S. tora at the chromosome level with 526 Mb (96%) assembled into 13 chromosomes. Comparison among related plant species shows that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identify a CHS-L gene contributing to the biosynthesis of anthraquinones. The S. tora reference genome will accelerate the discovery of biologically active anthraquinone biosynthesis pathways in medicinal plants.
Assuntos
Antraquinonas/metabolismo , Genoma de Planta , Proteínas de Plantas/genética , Senna/metabolismo , Antraquinonas/química , Vias Biossintéticas , Cromossomos de Plantas/genética , Cromossomos de Plantas/metabolismo , Proteínas de Plantas/metabolismo , Senna/química , Senna/genéticaRESUMO
Patatin-liked phospholipase A (pPLAs) are major lipid acyl hydrolases that participate in various biological functions in plant growth and development. Previously, a ginseng-derived pPLAIII homolog was reported to reduce lignin content in Arabidopsis. This led us to evaluate its possible usefulness as a biomass source in wood plant. Herein, we report that there are six members in the pPLAIII gene family in poplar. Overexpression of pPLAIIIß derived from ginseng resulted in a reduced plant height with radially expanded stem growth in hybrid poplars. Compared with the wild type (WT), the chlorophyll content was increased in the overexpression poplar lines, whereas the leaf size was smaller. The secondary cell wall structure in overexpression lines was also altered, exhibiting reduced lignification in the xylem. Two transcription factors, MYB92 and MYB152, which control lignin biosynthesis, were downregulated in the overexpression lines. The middle xylem of the overexpression line showed heavy thickening, making it thicker than the other xylem parts and the WT xylem, which rather could have been contributed by the presence of more cellulose in the selected surface area. Taken together, the results suggest that PgpPLAIIIß plays a role not only in cell elongation patterns, but also in determining the secondary cell wall composition.
Assuntos
Lignina/metabolismo , Panax/genética , Fosfolipases A/genética , Proteínas de Plantas/genética , Populus/genética , Madeira/crescimento & desenvolvimento , Sequência de Aminoácidos , Regulação da Expressão Gênica de Plantas , Lignina/genética , Panax/química , Fosfolipases A/química , Fosfolipases A/metabolismo , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Populus/crescimento & desenvolvimento , Alinhamento de Sequência , Madeira/genética , Xilema/genética , Xilema/crescimento & desenvolvimentoRESUMO
BACKGROUND: Prenyltransferases catalyze the sequential addition of isopentenyl diphosphate units to allylic prenyl diphosphate acceptors and are classified as either trans-prenyltransferases (TPTs) or cis-prenyltransferases (CPTs). The functions of CPTs have been well characterized in bacteria, yeast, and mammals compared to plants. The characterization of CPTs also has been less studied than TPTs. In the present study, molecular cloning and functional characterization of a CPT from a medicinal plant, Panax ginseng Mayer were addressed. METHODS: Gene expression patterns of PgCPT1 were analyzed by quantitative reverse transcription polymerase chain reaction. In planta transformation was generated by floral dipping using Agrobacterium tumefaciens. Yeast transformation was performed by lithium acetate and heat-shock for rer2Δ complementation and yeast-two-hybrid assay. RESULTS: The ginseng genome contains at least one family of three putative CPT genes. PgCPT1 is expressed in all organs, but more predominantly in the leaves. Overexpression of PgCPT1 did not show any plant growth defect, and its protein can complement yeast mutant rer2Δ via possible protein-protein interaction with PgCPTL2. CONCLUSION: Partial complementation of the yeast dolichol biosynthesis mutant rer2Δ suggested that PgCPT1 is involved in dolichol biosynthesis. Direct protein interaction between PgCPT1 and a human Nogo-B receptor homolog suggests that PgCPT1 requires an accessory component for proper function.
RESUMO
The effect of exogenously applied hydrogen peroxide on salt stress tolerance was investigated in Panax ginseng. Pretreatment of ginseng seedlings with 100 µM H2O2 increased the physiological salt tolerance of the ginseng plant and was used as the optimum concentration to induce salt tolerance capacity. Treatment with exogenous H2O2 for 2 days significantly enhanced salt stress tolerance in ginseng seedlings by increasing the activities of ascorbate peroxidase, catalase and guaiacol peroxidase and by decreasing the concentrations of malondialdehyde (MDA) and endogenous H2O2 as well as the production rate of superoxide radical (O2(-)). There was a positive physiological effect on the growth and development of salt-stressed seedlings by exogenous H2O2 as measured by ginseng dry weight and both chlorophyll and carotenoid contents. Exogenous H2O2 induced changes in MDA, O2(-), antioxidant enzymes and antioxidant compounds, which are responsible for increases in salt stress tolerance. Salt treatment caused drastic declines in ginseng growth and antioxidants levels; whereas, acclimation treatment with H2O2 allowed the ginseng seedlings to recover from salt stress by up-regulation of defense-related proteins such as antioxidant enzymes and antioxidant compounds.
Assuntos
Peróxido de Hidrogênio/farmacologia , Panax/efeitos dos fármacos , Tolerância ao Sal/efeitos dos fármacos , Antioxidantes/metabolismo , Ascorbato Peroxidases/biossíntese , Catalase/biossíntese , Panax/crescimento & desenvolvimento , Panax/metabolismo , Peroxidase/biossíntese , Plântula/efeitos dos fármacos , Estresse Fisiológico/efeitos dos fármacosRESUMO
Ginsenosides are glycosylated triterpenes that are considered to be important pharmaceutically active components of the ginseng (Panax ginseng 'Meyer') plant, which is known as an adaptogenic herb. However, the regulatory mechanism underlying the biosynthesis of triterpene saponin through the mevalonate pathway in ginseng remains unclear. In this study, we characterized the role of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) concerning ginsenoside biosynthesis. Through analysis of full-length complementary DNA, two forms of ginseng HMGR (PgHMGR1 and PgHMGR2) were identified as showing high sequence identity. The steady-state mRNA expression patterns of PgHMGR1 and PgHMGR2 are relatively low in seed, leaf, stem, and flower, but stronger in the petiole of seedling and root. The transcripts of PgHMGR1 were relatively constant in 3- and 6-year-old ginseng roots. However, PgHMGR2 was increased five times in the 6-year-old ginseng roots compared with the 3-year-old ginseng roots, which indicates that HMGRs have constant and specific roles in the accumulation of ginsenosides in roots. Competitive inhibition of HMGR by mevinolin caused a significant reduction of total ginsenoside in ginseng adventitious roots. Moreover, continuous dark exposure for 2 to 3 d increased the total ginsenosides content in 3-year-old ginseng after the dark-induced activity of PgHMGR1. These results suggest that PgHMGR1 is associated with the dark-dependent promotion of ginsenoside biosynthesis. We also observed that the PgHMGR1 can complement Arabidopsis (Arabidopsis thaliana) hmgr1-1 and that the overexpression of PgHMGR1 enhanced the production of sterols and triterpenes in Arabidopsis and ginseng. Overall, this finding suggests that ginseng HMGRs play a regulatory role in triterpene ginsenoside biosynthesis.
Assuntos
Genes de Plantas , Hidroximetilglutaril-CoA Redutases/genética , Panax/enzimologia , Panax/genética , Proteínas de Plantas/genética , Saponinas/biossíntese , Triterpenos/metabolismo , Arabidopsis/genética , Vias Biossintéticas/genética , Sequência Conservada , DNA Bacteriano/genética , Escuridão , Regulação da Expressão Gênica de Plantas , Ginsenosídeos/biossíntese , Ginsenosídeos/química , Hidroximetilglutaril-CoA Redutases/metabolismo , Modelos Biológicos , Especificidade de Órgãos/genética , Panax/crescimento & desenvolvimento , Proteínas de Plantas/metabolismo , Raízes de Plantas/enzimologia , Raízes de Plantas/genética , Plantas Geneticamente Modificadas , Transporte Proteico , Saponinas/química , Análise de Sequência de DNA , Frações Subcelulares/enzimologiaRESUMO
Protective effects of exogenous spermidine (Spd), activity of antioxygenic enzymes, and levels of free radicals in a well-known medicinal plant, Panax ginseng was examined. Seedlings grown in salinized nutrient solution (150 mM NaCl) for 7d exhibited reduced relative water content, plant growth, increased free radicals, and showing elevated lipid peroxidation. Application of Spd (0.01, 0.1, and 1mM) to the salinized nutrient solution showed increased plant growth by preventing chlorophyll degradation and increasing PA levels, as well as antioxidant enzymes such as CAT, APX, and GPX activity in the seedlings of ginseng. During salinity stress, Spd was effective for lowering the accumulation of putrescine (Put), with a significant increase in the spermidine (Spd) and spermine (Spm) levels in the ginseng seedlings. A decline in the Put level ran parallel to the higher accumulation of proline (Pro), and exogenous Spd also resulted in the alleviation of Pro content under salinity. Hydrogen peroxide (H2O2) and superoxide (O2(-)) production rates were also reduced in stressed plants after Spd treatment. Furthermore, the combined effect of Spd and salt led to a significant increase in diamine oxidase (DAO), and subsequent decline in polyamine oxidase (PAO). These positive effects were observed in 0.1 and 1mM Spd concentrations, but a lower concentration (0.01 mM) had a very limited effect. In summary, application of exogenous Spd could enhance salt tolerance of P. ginseng by enhancing the activities of enzyme scavenging system, which influence the intensity of oxidative stress.
Assuntos
Panax/efeitos dos fármacos , Panax/crescimento & desenvolvimento , Panax/metabolismo , Plântula/efeitos dos fármacos , Espermidina/farmacologia , Amina Oxidase (contendo Cobre)/metabolismo , Antioxidantes/metabolismo , Relação Dose-Resposta a Droga , Enzimas/metabolismo , Peróxido de Hidrogênio/metabolismo , Peroxidação de Lipídeos/efeitos dos fármacos , Oxirredutases atuantes sobre Doadores de Grupo CH-NH/metabolismo , Poliaminas/metabolismo , Prolina/metabolismo , Salinidade , Plântula/crescimento & desenvolvimento , Plântula/metabolismo , Espermidina/metabolismo , Espermina/metabolismo , Estresse Fisiológico/efeitos dos fármacos , Superóxidos/metabolismo , Poliamina OxidaseRESUMO
The differential transcript patterns of five antioxidant genes, four genes related to the ginsenoside pathway and five P450 genes related to defense mechanism were investigated in in vitro adventitious roots of Panax ginseng after exposure to two different concentrations of heavy metals for 7 days. PgSOD-1 and PgCAT transcription increased in a dose-dependent manner during the exposure to CuCl(2), NiCl(2), and CdCl(2), while all other tested scavenging enzymes didn't show significant increase during heavy metal exposure. Conversely, the mRNA transcripts of PgSQE, PgDDS were highly responsive to CuCl(2) compared to NiCl(2) exposure. However, the transcript profile of Pgß-AS was highly induced upon NiCl(2) treatment compared to CuCl(2) and CdCl(2) exposure. The expressions of PgCYP716A42, PgCYP71A50U, and PgCYP82C22 were regulated in similar manners, and all showed the highest transcript profile at 100 µM of CuCl(2), CdCl(2), and NiCl(2) except PgCYP71D184, which showed the highest transcript level when subjected to 10 µM CuCl(2) and NiCl(2). Thus it may suggest that in P. ginseng heavy metal interaction on cell membrane induced expression of various defense related genes via jasmonic acid pathway and also possesses cross talk networks with other defense related pathways.
Assuntos
Antioxidantes/metabolismo , Sistema Enzimático do Citocromo P-450/genética , Ginsenosídeos/biossíntese , Metais Pesados/toxicidade , Panax/efeitos dos fármacos , RNA Mensageiro/genética , Sequência de Bases , Primers do DNA , Etiquetas de Sequências Expressas , Reação em Cadeia da Polimerase em Tempo RealRESUMO
Korean ginseng (Panax ginseng C. A. Meyer) consists of nine cultivars from three Jakyung, Chungkyung, and Hwangsook lines. Among three previously identified PR-10 homologs from ginseng (PgPR10-1, PgPR10-2, and PgPR10-3), we found that the exact same sequence of PgPR10-2 exist in all tested nine cultivars. But a deletion and SNP was found in American ginseng (Panax quinquefolius). PR-10 proteins are known to be small and cytosolic, and showed similar three-dimensional structure. Here we show that the heterologous overexpression of PgPR10-1 in Arabidopsis showed enhanced resistance against Pseudomonas syringe, Fusarium oxysporum, and Botrytis cinerea and in-frame tagging with fluorescent protein showed its cytoplasm and nucleus localization. Protein-protein interaction of PgPR10-2 with PgPR10-1, PgPR10-2 and PgPR10-3 suggests that the PgPR10 proteins might form multimeric complexes in different cellular compartments to function in development and in defense-related mechanism. Differential response of PgPR10-1 and PgPR10-2 against different sets of biotic stresses in ginseng plant supports this notion.
Assuntos
Arabidopsis/genética , Arabidopsis/metabolismo , Panax/genética , Proteínas de Plantas/genética , Sequência de Aminoácidos , Arabidopsis/microbiologia , Sequência de Bases , Botrytis/patogenicidade , Sequência Conservada , DNA de Plantas/genética , Fusarium/patogenicidade , Vetores Genéticos , Dados de Sequência Molecular , Panax/metabolismo , Panax/microbiologia , Filogenia , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas , Pseudomonas syringae/patogenicidade , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Homologia de Sequência de Aminoácidos , Estresse FisiológicoRESUMO
Highly variable intergenic spacer and intron regions from nuclear and cytoplasmic DNA have been used for species identification. Noncoding internal transcribed spacers (ITSs) located in 18S-5.8S-26S, and 5S ribosomal RNA genes (rDNAs) represent suitable region for medicinal plant authentication. Noncoding regions from two cytoplasmic DNA, chloroplast DNA (trnT-F intergenic spacer region), and mitochondrial DNA (fourth intron region of nad7 gene) are also successfully applied for the proper identification of medicinal plants. Single-nucleotide polymorphism (SNP) sites obtained from the amplification of intergenic spacer and intron regions are properly utilized for the verification of medicinal plants in species level using multiplex PCR. Multiplex PCR as a variant of PCR technique used to amplify more than two loci simultaneously.
Assuntos
Reação em Cadeia da Polimerase Multiplex/métodos , Plantas Medicinais/genética , Polimorfismo de Nucleotídeo Único , DNA Intergênico , DNA Ribossômico/químicaRESUMO
Calmodulin (CaM), the predominant Ca(2+) receptors, is one of the best-characterized Ca(2+) sensors in all eukaryotes. In this study the role of CaM and the possible interrelationship between CaM and hydrogen peroxide (H(2)O(2)) in abscisic acid (ABA) induced antioxidant defense were investigated in the seedling of Panax ginseng. Treatment of ABA (100 µM) and H(2)O(2) (10 mM) increased the expression of Panax ginseng calmodulin gene (PgCaM) and significantly enhanced the expression of the antioxidant marker genes such as superoxide dismutase, ascorbate peroxidase, glutathione reductase and the activities of chloroplastic and cytosolic antioxidant enzymes. Pretreatments with two CaM antagonists, trifluoperazine (TFP), N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamide hydrochloride (W7) and inhibitor or scavenger, diphenyleneiodonium chloride, and dimethylthiourea of reactive oxygen species almost completely suppressed the up-regulation of antioxidant and PgCaM gene. Moreover, H(2)O(2) production and CaM content was almost completely inhibited by pretreatments with two CaM antagonists. In addition, the expressions of PgCaM gene under different biotic stress were analyzed at different time intervals. Thus it may suggests that CaM are involved in ABA-induced increased expression of PgCaM which triggers H(2)O(2) production through activating trans-plasma membrane NADPH oxidase, resulting in up-regulation of defense related antioxidant gene and also plays a pivotal role in defense response against pathogens.
Assuntos
Ácido Abscísico/metabolismo , Calmodulina/metabolismo , Peróxido de Hidrogênio/metabolismo , Panax/metabolismo , Plântula/metabolismo , Ácido Abscísico/química , Antioxidantes/metabolismo , Ascorbato Peroxidases/biossíntese , Cálcio/metabolismo , Calmodulina/antagonistas & inibidores , Calmodulina/química , Glutationa Redutase/biossíntese , Peróxido de Hidrogênio/química , NADPH Oxidases/biossíntese , NADPH Oxidases/metabolismo , Oniocompostos/farmacologia , Espécies Reativas de Oxigênio/metabolismo , Plântula/química , Plântula/enzimologia , Sulfonamidas/farmacologia , Superóxido Dismutase/biossíntese , Tioureia/análogos & derivados , Tioureia/farmacologia , Trifluoperazina/farmacologiaRESUMO
Pathogenesis-related 10 protein families (PgPR10 proteins) from ginseng are reported to have ribonuclease activity, conferring defense-related resistance against various stresses. Homology-based PCR using PgPR10-2 specific primers allowed for the isolation of two additional PgPR10 genes. PgPR10-1 is identical to the previously reported ribonuclease 1, while PgPR10-3 is a newly-discovered protein, suggesting that the PgPR10s are a multi-gene family. Differential organ-specific transcripts of PgPR10-1 and PgPR10-2 in the flower bud and root, respectively, indicate that there are tissue-specific functional roles for this gene family. Overexpression of PgPR10-2 in Arabidopsis conferred longer root length and a tolerant growth phenotype on NaCl-supplemented media. Further changes in transcriptional levels against sets of abiotic stressors suggest similar functional roles of PgPR10-1 in the root and predominantly in the flower organ based on its higher expression levels. Overall, this suggests that the manipulation of PgPR10 genes in plants can be used as valuable tool to enhance its physiological status.
Assuntos
Regulação da Expressão Gênica de Plantas/fisiologia , Panax/genética , Proteínas de Plantas/genética , Proteínas de Plantas/metabolismo , Sequência de Aminoácidos , Carotenoides/metabolismo , Clorofila/metabolismo , Análise por Conglomerados , Biologia Computacional , Ciclopentanos , Primers do DNA/genética , Flores/metabolismo , Regulação da Expressão Gênica de Plantas/genética , Vetores Genéticos/genética , Peróxido de Hidrogênio , Dados de Sequência Molecular , Oxilipinas , Raízes de Plantas/metabolismo , Reação em Cadeia da Polimerase , Reação em Cadeia da Polimerase em Tempo Real , República da Coreia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de SequênciaRESUMO
In plants heme containing cytochrome P450 (P450) is a superfamily of monooxygenases that catalyze the addition of one oxygen atom from O2 into a substrate, with a substantial reduction of the other atom to water. The function of P450 families is attributed to chemical defense mechanism under terrestrial environmental conditions; several are involved in secondary and hormone metabolism. However, the evolutionary relationships of P450 genes in Panax ginseng remain largely unknown. In the present study, data mining methods were implemented and 116 novel putative P450 genes were identified from Expressed Sequence Tags (ESTs) of a ginseng database. These genes were classified into four clans and 22 families by sequence similarity conducted at amino acid level. The representative putative P450 sequences of P. ginseng and known P450 family from other plants were used to construct a phylogenetic tree. By comparing with other genomes, we found that most of the P450 genes from P. ginseng can be found in other dicot species. Depending on P450 family functions, seven P450 genes were selected, and for that organ specific expression, abiotic, and biotic studies were performed by quantitative reverse transcriptase-polymerase chain reaction. Different genes were found to be expressed differently in different organs. Biotic stress and abiotic stress transcript level was regulated diversely, and upregulation of P450 genes indicated the involvement of certain genes under stress conditions. The upregulation of the P450 genes under methyl jasmonate and fungal stress justifies the involvement of specific genes in secondary metabolite biosynthesis. Our results provide a foundation for further elucidating the actual function and role of P450 involved in various biochemical pathways in P. ginseng.
Assuntos
Sistema Enzimático do Citocromo P-450/genética , Panax/classificação , Panax/enzimologia , Proteínas de Plantas/genética , Sistema Enzimático do Citocromo P-450/química , Sistema Enzimático do Citocromo P-450/metabolismo , Etiquetas de Sequências Expressas , Dados de Sequência Molecular , Panax/química , Panax/genética , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismoRESUMO
The regulation of reactive oxygen scavengers against biotic and abiotic conditions were investigated in the seedling of Panax ginseng C. A. Meyer. From the EST library we selected the antioxidant marker genes such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione peroxidase (GPX), and glutathione synthase (GS). The abiotic chilling, heat, osmotic, oxidative, and wounding stresses and biotic stresses with fungal pathogens were tested against 3-week-grown seedlings. The expression patterns of the genes were analyzed by means of real-time quantitative RT-PCR. The transcriptome result under abiotic stresses showed differential expression and elevated up-regulation of PgSOD, PgGPX, PgGS, and PgAPX, thus it may prove the generation of ROS in ginseng. Whereas, in biotic stress the up-regulation of transcript level merely based on the incompatible interactions. But PgAPX and PgCAT showed no significant change or slight down-regulation of transcript level during pathogen interaction. Thus it may suggest that in ginseng, plant-pathogen interaction triggers defense-related gene transcription via salicylic acid mediated signaling mechanism, and also possess crosstalk signaling networks between abiotic and biotic stress responses.
Assuntos
Sequestradores de Radicais Livres/metabolismo , Regulação da Expressão Gênica de Plantas/fisiologia , Panax/genética , Panax/metabolismo , Plântula/metabolismo , Estresse Fisiológico/fisiologia , Ascorbato Peroxidases , Catalase/metabolismo , Primers do DNA/genética , Perfilação da Expressão Gênica , Glutationa Peroxidase/metabolismo , Glutationa Sintase/metabolismo , Peroxidases/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estresse Fisiológico/genética , Superóxido Dismutase/metabolismoRESUMO
The role of plant chitinases in protecting plants against a variety of fungal pathogens is well established. In the present study, a cDNA clone containing a class I chitinase (Chi-1) gene, designated as PgChi-1, has been isolated from the oriental medicinal plant Panax ginseng. PgChi-1 is predicted to encode a protein of 34.9 kDa consisting of 323 amino acid residues. PgChi-1 was found to be expressed constitutively in all of the studied organs of ginseng plant. Under various abiotic stress treatments including Cu, H2O2, mannitol, SA, JA, and NaCl, the expression of PgChi-1 in plantlets and hairy roots increased significantly compared to the control. When different parts of root were analyzed, maximum level was observed in taproot. In addition, levels of PgChi-1 expression were compared between healthy root and fungal, bacterial, and nematode infected root. Significant increase of PgChi-1 was noticed in pathogen infected roots than healthy roots. This study revealed that PgChi-1 may protect the P. ginseng under both biotic and abiotic stress conditions.