Alleviation of allergic asthma by rosmarinic acid via gut-lung axis.

BACKGROUND: Asthma affects 3% of the global population, leading to over 0.25 million deaths. Due to its complexity, asthma is difficult to cure or prevent, and current therapies have limitations. This has led to a growing demand for alternative asthma treatments. We found rosmarinic acid (RosA) as a potential new drug candidate from natural medicine. However, RosA has poor bioavailability and remains mainly in the gastrointestinal tract after oral administration, suggesting the involvement of gut microbiota in its bioactivity. PURPOSE: To investigate the mechanism of RosA in alleviating allergic asthma by gut-lung axis. METHODS: We used 16S rRNA gene sequencing and metabolites analysis to investigate RosA's modulation of gut microbiota. Techniques of molecular biology and metabolomics were employed to study the pharmacological mechanism of RosA. Cohousing was used to confirm the involvement of gut microbiota in RosA-induced improvement of allergic asthma. RESULTS: RosA decreased cholate levels from spore-forming bacteria, leading to reduced 5-hydroxytryptamine (5-HT) synthesis, bronchoconstriction, vasodilation, and inflammatory cell infiltration. It also increased short-chain fatty acids (SCFAs) levels, facilitating the expression of intestinal tight junction proteins to promote intestinal integrity. SCFAs upregulated intestinal monocarboxylate transporters (MCTs), thereby improving their systemic delivery to reduce Th2/ILC2 mediated inflammatory response and suppress eosinophil influx and mucus production in lung. Additionally, RosA inhibited lipopolysaccharide (LPS) production and translocation, leading to reduced TLR4-NFκB mediated pulmonary inflammation and oxidative stress. CONCLUSIONS: The anti-asthmatic mechanism of oral RosA is primarily driven by modulation of gut microbiota-derived 5-HT, SCFAs, and LPS, achieving a combined synergistic effect. RosA is a safe, effective, and reliable drug candidate that could potentially replace glucocorticoids for asthma treatment.

Pressure/colorimetric dual-readout immunochromatographic test strip for point-of-care testing of aflatoxin B1.

Immunochromatographic test strip (ITS) for point-of-care testing (POCT) has attracted prominent attention due to the advantages including rapid response, low cost and good portability. Here, we developed a sensitive ITS for detecting aflatoxin B1 (AFB1) by using dendritic platinum nanoparticles (DPNs) as novel pressure/colorimetric dual-readout probes. DPNs-labeled antibody of AFB1 were used as the signal tracer of the immunochromatographic process. After 10-min competitive immunoreaction, black color appeared on the test line of ITS due to the accumulation of DPNs, which was observed visually as a colorimetric readout for qualitation purpose. Furthermore, DPNs with peroxidase-like activity caused decomposition of hydrogen peroxide aqueous solution to produce pressure change signal in vials, which was detected by a hand-held pressure meter for quantitation purpose. With the pressure readout mode, the detection range was 0.05-10 ng mL-1, and the detection limit was 0.03 ng mL-1 (S/N = 3) for AFB1. The proposed ITS was successfully utilized for detecting AFB1 in herbal medicine samples, and the acceptable recoveries of 93.77-114.09% indicated the reliability for real sample detection. It provides a new avenue for POCT with great application potential in various area including drug and food quality control, pollutants monitoring as well as medical diagnosis.