RESUMO
Macrophages are the main source of cytokines in atherosclerotic plaques. Modified low-density lipoproteins may stimulate macrophages to produce large quantities of proinflammatory cytokines that promote atherosclerosis. Berberine is the main component of the traditional Chinese medicine umbellatine, which has a widespread effect and was used to treat many diseases clinically. Our previous study found that berberine could increase adipophilin expression in macrophages, which is a target gene of PPARgamma. PPARgamma agonist could decrease proinflammatory cytokines in macrophage. In this study, we investigated the effects and the mechanism of action of berberine on the expression and secretion of TNFalpha, MCP-1, and IL-6 in vitro to identify new pharmacological actions of berberine. The results of RT-PCR and ELISA shows that berberine may inhibit the expression and secretion of the tumor necrosis factor alpha (TNFalpha), monocyte chemoattractant protein 1 (MCP-1), and interleukin-6 (IL-6) in macrophages stimulated by acetylated low-density lipoprotein (AcLDL), whereas the peroxisome proliferator-activated receptor gamma (PPARgamma) inhibitor GW9662 could attenuate this effect of berberine. This study demonstrates that berberine may inhibit the expression and production of TNF-alpha, MCP-1, and IL-6 in AcLDL-stimulated macrophages. This effect might be partially mediated through PPARgamma activity.
Assuntos
Berberina/farmacologia , Quimiocina CCL2/genética , Interleucina-6/genética , Macrófagos/efeitos dos fármacos , PPAR gama/metabolismo , Fator de Necrose Tumoral alfa/genética , Anilidas/farmacologia , Aterosclerose/tratamento farmacológico , Aterosclerose/metabolismo , Berberina/toxicidade , Linhagem Celular , Humanos , Hipoglicemiantes/farmacologia , Hipoglicemiantes/toxicidade , Lipoproteínas LDL/metabolismo , Lipoproteínas LDL/farmacologia , Macrófagos/citologia , Macrófagos/fisiologia , PPAR gama/antagonistas & inibidores , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Rosiglitazona , Tiazolidinedionas/farmacologia , Tiazolidinedionas/toxicidadeRESUMO
The objective of this study was to investigate the effect of ATP7B antisense oligodeoxynucleotides (ASODNs) on regulating the sensitivity to cisplatin in ovarian carcinoma cell line SKOV3ip1. The ATP7B ASODNs and the corresponding sense oligodeoxynucleotide (SODN) as control were transfected into SKOV3ip1 cells by lipofectamine-2000. The changes of ATP7B were detected by reverse transcription-polymerase chain reaction, flow cytometry, and Western blotting. The survival rate of the SKOV3ip1 cells was assessed by MTT assay. Compared with nontransfected cell, the transfer of ASODN/lipofectin (LF) into SKOV3ip1 cells resulted in (1) 73.70% and 48.30% reduction of ATP7B in messenger RNA and protein, respectively, (2) an obviously decreased intracellular fluorescence intensity from 79.42 to 50.87 (P < 0.01), and (3) a decreased IC(50) value for cisplatin from 126.63 to 80.90 micromol/L (P < 0.01), while no significant changes were detected for groups treated with SODN/LF and LF only. ASODN transfection can inhibit the expression of ATP7B and increase the cisplatin sensitivity in SKOV3ip1 cells.
Assuntos
Adenosina Trifosfatases/antagonistas & inibidores , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Carcinoma/tratamento farmacológico , Proteínas de Transporte de Cátions/antagonistas & inibidores , Cisplatino/administração & dosagem , Resistencia a Medicamentos Antineoplásicos/efeitos dos fármacos , Oligodesoxirribonucleotídeos Antissenso/administração & dosagem , Neoplasias Ovarianas/tratamento farmacológico , Adenosina Trifosfatases/genética , Adenosina Trifosfatases/metabolismo , Carcinoma/genética , Carcinoma/metabolismo , Carcinoma/patologia , Proteínas de Transporte de Cátions/genética , Proteínas de Transporte de Cátions/metabolismo , Linhagem Celular Tumoral , ATPases Transportadoras de Cobre , Avaliação Pré-Clínica de Medicamentos , Resistencia a Medicamentos Antineoplásicos/genética , Sinergismo Farmacológico , Feminino , Regulação Neoplásica da Expressão Gênica , Humanos , Neoplasias Ovarianas/genética , Neoplasias Ovarianas/metabolismo , Neoplasias Ovarianas/patologia , RNA Mensageiro/metabolismo , TransfecçãoRESUMO
Observation on 30 patients with fissured tongue (FT) and 42 normal subjects, through the measurement of flow and the elements of saliva, pH value, the Na and K content, SIgA, it was shown that: in FT patients, the flow of saliva and pH decreased, while the Na/K ratio and the content of SIgA increased (each P < 0.01, compared with the normal). It was also showed that the formation of FT has to a certain extent something to do with the electrolyte disturbance in aqueous solution, malnutrition, as well as immunity and endocrinic function of patients.
Assuntos
Saliva/metabolismo , Língua Fissurada/metabolismo , Feminino , Humanos , Concentração de Íons de Hidrogênio , Imunoglobulina A Secretora/análise , Masculino , Pessoa de Meia-Idade , Potássio/análise , Saliva/química , Salivação , Sódio/análiseRESUMO
Zinc concentrations in plasma and hair were measured in 703 children, aged between 1 and 6 yr, and correlated with parameters of physical development. In the first group of 187 children brought to the Child Health Clinic for routine observation there was a positive correlation of hair zinc content and height for age, with an increased prevalence of low hair zinc content in children of shorter stature. A second group of 303 children in nurseries and kindergartens in Beijing exhibited a hair zinc content of 92 micrograms/g, and 34% of these had very low zinc values below 70 micrograms/g. The third group consisted of 213 children who were brought into the outpatient clinic for a variety of complaints, including pica, anorexia, and poor growth; these had significantly lower values of zinc in hair and plasma than well-nourished children and responded to zinc supplementation with improvement of growth and the disappearance of pica and anorexia. These results suggest that the diet consumed by the population studied may be marginal or inadequate in its content of available zinc.