RESUMO
The leaves of Elaeagnus pungens were extracted with 70% ethanol and successively purified by column chromatography. Seven constituents were obtained and characterized, all of which belong to the class of flavonol glycosides. Their structures were elucidated on the basis of spectroscopic methods including 1D/2D NMR and MS analysis techniques. The seven flavonol glycosides were determined as kaempferol 3-O-ß-d-glucopyranosyl-(1 â 3)-α-l-rhamnopyranosyl-(1 â 6)-[α-l-rhamnopyranosyl(1 â 2)]-ß-d-galactopyranoside (1), isorhamnetin 3-O-ß-d-glucopyranosyl-(1 â 3)-α-l-rhamnopyranosyl-(1 â 6)-ß-d-galactopyranoside (2), together with five known compounds, respectively. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliu-m bromide assay in vitro showed that the isolated flavonol glycosides showed no proliferation activity in the asthma airway smooth muscle cells, comparing with solvent as the control group.
Assuntos
Medicamentos de Ervas Chinesas/isolamento & purificação , Elaeagnaceae/química , Flavonóis/isolamento & purificação , Glicosídeos/isolamento & purificação , Medicamentos de Ervas Chinesas/química , Flavonóis/química , Flavonóis/farmacologia , Glicosídeos/química , Glicosídeos/farmacologia , Quempferóis/química , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Folhas de Planta/química , Quercetina/análogos & derivados , Quercetina/químicaRESUMO
OBJECTIVE: To study water-soluble chemical constituents from the leaves of Elaeagnus pungens. METHOD: Chemical constituents of E. pungens leaves were separated by a combination of macroporous resin column chromatography, reverse phase silica gel column chromatography, Sephadex LH-20 column chromatography and semi-preparative HPLC. Their structures were identified on the basis of physicochemical properties using the spectral method. RESULT: The two compounds were separated from E. pungens leaves and identified as kaempferol 3-O-P-D-glucopyranosyl- (1-->3)-alpha-L-rhamn-opyranosyl-(1-->6) -/3-D-galactopyranoside (1), kaempferol 3-O-P-D-glucopyranosyl-(1-->3)-alpha-L-rhamnopyranosyl-(1-->6)-beta-D-glucopyranoside-7-O-beta-D-glucopyranoside (2). CONCLUSION: Compound 2 separated from E. pungens leaves was a new compound.