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This study aimed to explore the co-application of MnSO4 (Mn) and biochar (BC) in nitrogen conversion during the composting process. A 70-day aerobic composting was conducted using swine slurry, supplemented with different levels of Mn (0, 0.25%, and 0.5%) and 5% BC. The results demonstrated that the treatment with 0.5MnBC had the highest levels of NH4+-N (3.07 g kg-1), TKN (29.90 g kg-1), and NO3--N (1.94 g kg-1) among all treatments. Additionally, the 0.5MnBC treatment demonstrated higher urease, protease, nitrate reductase, and nitrite reductase activities than the other treatments, with the peak values of 18.12, 6.96, 3.57, and 15.14 mg g-1 d-1, respectively. The addition of Mn2+ increased the total organic nitrogen content by 29.59%-47.82%, the acid hydrolyzed ammonia nitrogen (AN) content by 13.84%-57.86% and the amino acid nitrogen (AAN) content by 55.38%-77.83%. The richness of Chloroflexi and Ascomycota was also enhanced by the simultaneous application of BC and Mn. Structural equation modeling analysis showed that Mn2+ can promote the conversion of Hydrolyzed Unknown Nitrogen (HUN) into AAN, and there is a positive association between urease and NH4+-N according to redundancy analysis. Firmicutes, Basidiomycota, and Mortierellomycota showed significant positive correlations with ASN, AN, and NH4+-N, indicating their crucial roles in nitrogen conversion. This study sheds light on promoting nitrogen conversion in swine slurry composting through the co-application of biochar and manganese sulfate.
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Compostos de Manganês , Nitrogênio , Solo , Sulfatos , Animais , Suínos , Nitrogênio/metabolismo , Urease , Esterco , Carvão VegetalRESUMO
In this work, a novel boehmite-modified carbon adsorbent (BMCC) derived from moldy corn was used for simultaneous removal of P and bisphenol A (BPA) from livestock wastewater. The results showed that BMCC had a high specific surface area (308.82 m2/g) with boehmite nanoparticles anchored on its surface. BMCC showed high P and BPA decontamination capabilities (40.98 mg/g for P and 54.65 mg/g for BPA by Langmuir model). The adsorbed amount of P declined as pH increased from 4 to 10, while the adsorbed amount of BPA remained steady until pH increased to 10. After 6 cycles of BMCC use, the P and BPA adsorption efficiencies reduced by 21.75 % and 19.41 %, respectively. The adsorption of P was dominated by electrostatic attraction and complexation, while the adsorption of BPA was controlled by hydrogen bonding, electrostatic interaction, and π-π association. In conclusion, BMCC is an effective treatment for decontaminating P- and BPA-contaminated livestock wastewater.
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Hidróxido de Alumínio , Óxido de Alumínio , Carbono , Fenóis , Poluentes Químicos da Água , Animais , Águas Residuárias , Gado , Fósforo , Descontaminação , Cinética , Compostos Benzidrílicos , Adsorção , Concentração de Íons de HidrogênioRESUMO
Introduction: Type 2 diabetes (T2D) is a multifactorial complex chronic disease with a high prevalence worldwide, and Type 2 diabetes patients with different comorbidities often present multiple phenotypes in the clinic. Thus, there is a pressing need to improve understanding of the complexity of the clinical Type 2 diabetes population to help identify more accurate disease subtypes for personalized treatment. Methods: Here, utilizing the traditional Chinese medicine (TCM) clinical electronic medical records (EMRs) of 2137 Type 2 diabetes inpatients, we followed a heterogeneous medical record network (HEMnet) framework to construct heterogeneous medical record networks by integrating the clinical features from the electronic medical records, molecular interaction networks and domain knowledge. Results: Of the 2137 Type 2 diabetes patients, 1347 were male (63.03%), and 790 were female (36.97%). Using the HEMnet method, we obtained eight non-overlapping patient subgroups. For example, in H3, Poria, Astragali Radix, Glycyrrhizae Radix et Rhizoma, Cinnamomi Ramulus, and Liriopes Radix were identified as significant botanical drugs. Cardiovascular diseases (CVDs) were found to be significant comorbidities. Furthermore, enrichment analysis showed that there were six overlapping pathways and eight overlapping Gene Ontology terms among the herbs, comorbidities, and Type 2 diabetes in H3. Discussion: Our results demonstrate that identification of the Type 2 diabetes subgroup based on the HEMnet method can provide important guidance for the clinical use of herbal prescriptions and that this method can be used for other complex diseases.
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The objective of this research was to elucidate the effect of varying proportions of magnesite (MS) addition - 0% (T1), 2.5% (T2), 5% (T3), 7.5% (T4), and 10% (T5) - on nitrogen transformation and bacterial community dynamics during pig manure composting. In comparison to T1 (control), MS treatments amplified the abundance of Firmicutes, Actinobacteriota, and Halanaerobiaeota, bolstered the metabolic functionality of associated microorganisms, and enhanced the nitrogenous substance metabolic pathway. A complementary effect in core bacillus species played a key role in nitrogen preservation. Compared to T1, 10% MS demonstrated the most substantial influence on composting because Total Kjeldahl Nitrogen increased by 58.31% and NH3 emission decreased by 41.52%. In conclusion, 10% MS appears to be optimal for pig manure composting, as it can augment microbial abundance and mitigate nitrogen loss. This study offers a more ecologically sound and economically viable method for curtailing nitrogen loss during composting.
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Compostagem , Nitrogênio , Animais , Suínos , Esterco , Solo , BactériasRESUMO
The potential of sulphur (S), MgSO4 (Mg), and KH2PO4 (P) in nitrogen retention, ammonia emission decrease, and microbial community succession during composting needs to be investigated. To achieve this, different levels of S (0, 0.2, 0.4, 0.6, and 0.8% in dry weight) plus Mg and P (S + Mg + P) were progressively added in 70 days pig manure aerobic composting. The results revealed that the amendment increased salinity and lowered pH and dephytotoxication of the product with the increase of S amount. However, no significant inhibition effects were observed on the evolution of the thermophilic phase and product maturity. In addition, the amendment significantly reduced the total NH3 and N2O emissions by 29.66%-58.81% and 20.6%-56.7%, increased NH4+ level by 17.22%-73.21% in thermophilic phase and NO3- content by 26.17%-57.48% in a mature phase, and elevated the total Kjeldahl nitrogen content by 34.28%-46.6% during the composting. In addition, compared to the control, the supplement markedly encouraged the formation of guanite in the compost product. The S addition stimulated the growth of Anseongella, Actinomadura, Chelativorans, Castellaniella, Luteimonas, and Steroidobacter microbial communities which functioned well in the degradation of nitrogen-containing compounds and organic matter. Evidence from Redundancy Analysis, Firmicutes, Myxococcus, Chloroflexi, Gemmatimonadota, and Deinococcota showed positive correlations with pH. These results imply that adding S-Mg-P amendment encourages the population and activity of specific functional microorganisms, and facilitated the ammonia emission reduction by lowering pH and thus reserved nitrogen through the formation of guanite during composting. The investigation of bacterial community abundance and environmental variables at the phylum and genus levels over time revealed that adding of 0.6% S in conjunction with P and Mg minerals was suitable for nitrogen loss mitigation in composting. The findings suggest using S + Mg + P supplement to conserve nitrogen in pig dung aerobic composting.
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Amônia , Compostagem , Suínos , Animais , Esterco , Solo , Nitrogênio , Enxofre , BactériasRESUMO
A versatile drug delivery system (DDS) enabling highly effective and targeting oncotherapy has always been of great significance in medical research. In the development of a stimuli-responsive DDS, compared with a single-factor stimulation DDS, a multifactor activation DDS has higher therapeutic specificity between diseased and normal tissue, but there are challenges in drug-release efficiency and united targeting cancer therapy. Herein, a novel dual-microRNA (dual-miRNA)-mediated 1:N-amplified DDS is fabricated. The gold nanocage (AuNC) was synthesized and used as a carrier. A DNA bridge motif as a nanolock (DNA bridge nanolock) was designed and modified on the surface of AuNCs, which could seal the holes of AuNCs. Using the dual-miRNAs as a pair of master keys, through DNA strand migration and DNAzyme self-assembly, a cell endogenous substance Mg2+-dependent DNAzyme cyclic shear reaction could perform the function of the master keys to open multiple locks for the enhanced release of doxorubicin from the AuNCs. In addition, under near-infrared irradiation, via absorption of light and heat release, the AuNC is activated to perform the function of photothermal therapy. Thereby, the system achieves precise chemo-photothermal therapy. Using the in vitro and in vivo anti-tumor analysis, the DDS could be proved to present a novel design of enhanced and targeted drug-release system for highly effective cancer therapy.
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DNA Catalítico , Hipertermia Induzida , Nanopartículas , Neoplasias , Linhagem Celular Tumoral , Doxorrubicina/farmacologia , Doxorrubicina/uso terapêutico , Sistemas de Liberação de Medicamentos , Liberação Controlada de Fármacos , Humanos , Neoplasias/tratamento farmacológico , Neoplasias/patologia , Fototerapia , Terapia FototérmicaRESUMO
The effects of magnesite (MS) on fungi communities and the core fungi complementarity during pig manure (PM) composting were explored. Different dosage of MS [0% (T1), 2.5% (T2), 5% (T3), 7.5% (T4) and 10% (T5)] as amendments mixed with PM for 42 days composting. The results showed the dominant of phyla were Ascomycota (78.87%), Neocallimastigomycota (41.40%), Basidiomycota (30.81%) and Aphelidiomycota (29.44%). From day 7 to 42, the abundance of Ascomycota and Aphelidiomycota were increased from 7.75% to 42.41% to 57.27%-78.87% and 0-0.70% to 11.73%-29.44% among all treatments. Nevertheless, the phyla abundance of Neocallimastigomycota and Basidiomycota decreased from day 7 to 42. The co-occurrence network indicated that the high additive amendment could enhance the core fungi complementarity effects capacity. The 10% MS addition was a promisable candidate to optimum fungal communities, and causing a better compost quality. This study illustrated the potential and fungi communities changing of MS as additives in composting.
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Ascomicetos , Basidiomycota , Compostagem , Micobioma , Animais , Magnésio , Esterco/microbiologia , Solo , SuínosRESUMO
Management of industrial hazardous waste is of great concern. Recently, aluminum rich drinking water treatment residuals (Al-WTR) received considerable attention as a low-cost immobilizing agent for toxic elements in soils. However, the suitability and effectiveness of modified Al-WTR as stabilizing agent for toxic metals such as Cu and Pb in mining soil is not assessed yet. We examined the impact of different doses (0, 0.5, 1.5, and 2.5%) of raw and Fe/Mn- and P- modified Al-WTR on the bioavailability and uptake of Cu and Pb by ryegrass in Cu and Pb contaminated mining soil. The addition of Fe/Mn-and P- modified Al-WTR to the soil reduced significantly the concentrations of Pb (up to 60% by Fe/Mn-Al-WTR and 32% by P-Al-WTR) and Cu (up to 45% by Fe/Mn-Al-WTR and 18% by P-Al-WTR) in the shoots and roots of ryegrass as compared to raw Al-WTRs and untreated soil. Our results demonstrate that modification of the raw Al-WTR increased its pH, CEC, specific surface area, active functional groups (Fe-O and Mn-O), and thus increased its immobilization efficiency. Our results highlight the potential of the modified Al-WTR, particularly the Fe/Mn-Al-WTR, for the remediation of Cu and Pb contaminated soils and recommend field scale verification.
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Água Potável , Poluentes do Solo , Purificação da Água , Chumbo , Mineração , Solo , Poluentes do Solo/análiseRESUMO
BACKGROUND: Biodiversity-based agricultural systems can improve production efficiency and sustainability, with fewer negative environmental impacts and lower use of external inputs. Mixed-cropping and rice-duck co-culture have been shown to produce ecological benefits and to have positive effects on paddy soil. However, the effects of a combination of mixed cropping with different rice cultivars and duck co-culture on soil nutrients availability and grain yields have not been evaluated. A paddy field experiment was carried out over two rice growing seasons to test these effects. RESULTS: Several combinations of rice cultivars, when integrated with duck co-culture, significantly increased the soil organic matter and total nitrogen contents during the rice growing seasons, as compared to mono-cropping systems. In mixed-cropping combined with duck co-culture (MCDC) systems, the soil alkali-hydrolyzable nitrogen content ranged from 4.33% to 17.86% higher than that in mono-cropping systems. Similar increases were found for soil available phosphorus (8.71-15.91%) and soil available potassium (8.65-39.43%) contents. Furthermore, MCDC produced higher grain yields and harvest indexes for both study seasons. CONCLUSION: The integration of MCDC systems had positive effects on soil nutrient contents of paddy fields, which could, in turn, lead to yield enhancements, as well as additional income for farmers in the form of organic duck meat. © 2019 Society of Chemical Industry.
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Produção Agrícola/métodos , Patos/crescimento & desenvolvimento , Oryza/crescimento & desenvolvimento , Solo/química , Animais , China , Fertilizantes/análise , Nitrogênio/análise , Nitrogênio/metabolismo , Oryza/metabolismo , Fósforo/análise , Fósforo/metabolismo , Potássio/análise , Potássio/metabolismo , Estações do AnoRESUMO
The marine gastropod Hemifusus tuba is served as a luxury food in Asian countries and used in traditional Chinese medicine to treat lumbago and deafness. The lack of genomic data on H. tuba is a barrier to aquaculture development and functional characteristics of potential bioactive molecules are poorly understood. In the present study, we used high-throughput sequencing technologies to generate the first transcriptomic database of H. tuba. A total of 41 unique conopeptides were retrieved from 44 unigenes, containing 6-cysteine frameworks belonging to four superfamilies. Duplication of mature regions and alternative splicing were also found in some of the conopeptides, and the de novo assembly identified a total of 76,306 transcripts with an average length of 824.6 nt, of which including 75,620 (99.1%) were annotated. In addition, simple sequence repeats (SSRs) detection identified 14,000 unigenes containing 20,735 SSRs, among which, 23 polymorphic SSRs were screened. Thirteen of these markers could be amplified in Hemifusus ternatanus and seven in Rapana venosa. This study provides reports of conopeptide genes in Buccinidae for the first time as well as genomic resources for further drug development, gene discovery and population resource studies of this species.
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Organismos Aquáticos/genética , Conotoxinas/genética , Gastrópodes/genética , Transcriptoma/genética , Animais , Perfilação da Expressão Gênica/métodos , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Repetições de Microssatélites/genética , Anotação de Sequência Molecular/métodosRESUMO
Ginkgo biloba (a herbal product) has long been used in traditional Chinese medicine to treat cerebrovascular and cardiovascular diseases. Ginkgolide B is one of the important pharmacologically active components of Ginkgo biloba. Dimethylaminoethyl ginkgolide B (a novel ginkgolide B derivative) has been developed to overcome the poor water-solubility of natural drugs and to achieve higher bioavailability. This study investigated the potential effects of dimethylaminoethyl ginkgolide B on pregnant dams and embryo-fetal development in Sprague-Dawley rats and New Zealand white rabbits following maternal exposure on gestational day (GD) 6-15 and GD 6-18, respectively. Dimethylaminoethyl ginkgolide B was administered by intravenous injection to pregnant rats (0, 10, 30 and 100 mg kg-1 d-1) and rabbits (0, 6, 18 and 60 mg kg-1 d-1). Maternal toxicity signs, such as lower maternal body weight gain and food consumption, were observed at 100 mg kg-1 d-1 in rats and 60 mg kg-1 d-1 in rabbits. The developmental toxic effects included a decrease in fetal and placental weights, increased incidences of skeletal variations and delay in fetal ossification. Fetal growth and development were affected by dimethylaminoethyl ginkgolide B in the high-dose group in rabbits. The no-observed-adverse-effect level of dimethylaminoethyl ginkgolide B is considered to be 30 mg kg-1 d-1 for rats and 18 mg kg-1 d-1 for rabbits.
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Metal oxide-Carbon composites have been developed tailoring towards specific functionalities for removing pollutants from contaminated environmental systems. In this study, we synthesized a novel CaO-MgO hybrid carbon composite for removal of phosphate and humate by co-pyrolysis of dolomite and sawdust at various temperatures. Increasing of pyrolysis temperature to 900⯰C generated a composite rich in carbon, CaO and MgO particles. Phosphate and humate can be removed efficiently by the synthesized composite with the initial solution in the range of pHâ¯3.0-11.0. The phosphate adsorption was best fitted by pseudo-second-order kinetic model, while the humate adsorption followed the pseudo-second-order and the intra-particle diffusion kinetic models. The maximum adsorption capabilities quantified by the Langmuir isotherm model were up to 207â¯mg phosphorus (or 621â¯mg phosphate) and 469â¯mg humate per one-gram composite used, respectively. Characterization of composites after adsorption revealed the contributions of phosphate crystal deposition and electrostatic attraction on the phosphate uptake and involvement of πâ¯-â¯π interaction in the humate adsorption. The prepared composite has great potential for recovering phosphorus from wastewater, and the phosphate sorbed composite can be employed as a promising phosphorus slow-releasing fertilizer for improving plant growth.
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Fosfatos/análise , Fósforo/química , Eliminação de Resíduos Líquidos/métodos , Poluentes Químicos da Água/análise , Adsorção , Carbono , Cinética , Fosfatos/química , Fósforo/análise , Reciclagem , Poluentes Químicos da Água/químicaRESUMO
BACKGROUND: By the search for new natural compounds with beneficial health effects, cephalopod ink has been considered as an attempt to develop new drugs and functional foods, which is an especially active field in Asia, where cephalopods are a major fishery catch, for which ink sacs are a bi-product and where homeopathic medicine has deep roots. There is a demand to evaluate the safety and influence to the organism. The specific composition and relative abundance of the gut microbiota, which is potentially a major modulator of host metabolism, drives the interaction between functional foods and host health. We explore the effects of melanin from Sepiella Maindroni, most common cuttlefish in China, on the intestinal microbiome of mice. RESULTS: ICR mice were randomly divided four groups, which were normal group (S), low melanin dose group (D; 120 mg/kg), medium melanin dose group (Z; 240 mg/kg), and high melanin dose group (G; 480 mg/kg). Melanin was delivered for 28 consecutive days. Fecal samples were used to generate 7715 operational taxonomic units (OTUs) via high-throughput sequencing. There were significant shifts in relative abundance of the dominant taxa at the phylum, class, order, family, and genus levels following melanin treatment. CONCLUSIONS: MSMI had no significant effect on the structure of intestinal flora in mice. The main effect was in the proportion of dominant bacterial communities. The effect positively correlated with the dose. From a health point of view, the use of melanin does not cause intestinal flora disorder. Our results may have important implications for MSMI as functional food component and potential therapeutic for manipulating gut microbiota.
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Bactérias/classificação , Decapodiformes/metabolismo , Microbioma Gastrointestinal/efeitos dos fármacos , Melaninas/administração & dosagem , Animais , Bactérias/genética , Relação Dose-Resposta a Droga , Fezes/microbiologia , Sequenciamento de Nucleotídeos em Larga Escala , Melaninas/farmacologia , Camundongos , Camundongos Endogâmicos ICR , Filogenia , RNA Bacteriano/genética , RNA Ribossômico 16S/genética , Distribuição Aleatória , Análise de Sequência de RNARESUMO
C-type lectins (CTLs) are a family of calcium-dependent carbohydrate-binding proteins. In the present study, a novel C-type lectin (designated as PtCTL1) was identified and characterized from Portunus trituberculatus. The full-length cDNA of PtCTL1 was of 702 bp, containing a 5' untranslated region (UTR) of 91 bp, a 3' UTR of 110 bp with a poly (A) tail, and an open reading frame (ORF) of 501 bp encoding a polypeptide of 166 amino acids with a putative signaling peptide of 21 amino acids. A C-type lectin carbohydrate-recognition domain (CRD) containing four conserved cysteines was identified in the amino acid sequence of PtCTL1. The cDNA fragment encoding the mature peptide of PtCTL1 was recombined into pET-21a(+) with a C-terminal hexa-histidine tag fused in-frame and expressed in Escherichia coli Origami (DE3). The recombinant PtCTL1 (rPtCTL1) can agglutinate all the tested bacteria, including three Gram-positive bacterial strains and three Gram-negative bacterial strains. In addition, erythrocyte agglutination and LPS-binding activity were observed in a Ca2+-dependent manner. The erythrocyte agglutination was inhibited by EDTA, indicating that PtCTL1 was Ca2+-dependent. The mRNA transcripts of PtCTL1 were detected mainly in the tissues of hepatopancreas and hemocytes and its levels were significantly up-regulated in hemocytes following Vibrio alginolyticus challenge. These results indicate that PtCTL1 may function as a pattern recognition receptor (PRR) for protecting P. trituberculatus from bacterial infection. Moreover, such findings also provide evidence for further understanding the innate immunology of invertebrate.
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Proteínas de Artrópodes/genética , Braquiúros/genética , Braquiúros/imunologia , Lectinas Tipo C/genética , Vibrio alginolyticus/fisiologia , Aglutinação , Sequência de Aminoácidos , Animais , Proteínas de Artrópodes/química , Proteínas de Artrópodes/metabolismo , Sequência de Bases , Braquiúros/efeitos dos fármacos , Braquiúros/microbiologia , Clonagem Molecular , DNA Complementar/genética , DNA Complementar/metabolismo , Testes de Hemaglutinação , Lectinas Tipo C/química , Lectinas Tipo C/metabolismo , Lipopolissacarídeos/farmacologia , Filogenia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Alinhamento de Sequência , Distribuição TecidualRESUMO
Soil contamination with heavy metals due to mining activities poses risks to ecological safety and human well-being. Limited studies have investigated heavy metal pollution due to artisanal mining. The present study focused on soil contamination and the health risk in villages in China with historical artisanal mining activities. Heavy metal levels in soils, tailings, cereal and vegetable crops were analyzed and health risk assessed. Additionally, a botany investigation was conducted to identify potential plants for further phytoremediation. The results showed that soils were highly contaminated by residual tailings and previous mining activities. Hg and Cd were the main pollutants in soils. The Hg and Pb concentrations in grains and some vegetables exceeded tolerance limits. Moreover, heavy metal contents in wheat grains were higher than those in maize grains, and leafy vegetables had high concentrations of metals. Ingestion of local grain-based food was the main sources of Hg, Cd, and Pb intake. Local residents had high chronic risks due to the intake of Hg and Pb, while their carcinogenic risk associated with Cd through inhalation was low. Three plants (Erigeron canadensis L., Digitaria ciliaris (Retz.) Koel., and Solanum nigrum L.) were identified as suitable species for phytoremediation.
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Produtos Agrícolas/crescimento & desenvolvimento , Monitoramento Ambiental/métodos , Ouro , Metais Pesados/análise , Mineração , Poluentes do Solo/análise , Biodegradação Ambiental , China , Digitaria/crescimento & desenvolvimento , Erigeron/crescimento & desenvolvimento , Contaminação de Alimentos/análise , Contaminação de Alimentos/prevenção & controle , Humanos , Medição de Risco , Solo/química , Solo/normas , Solanum/crescimento & desenvolvimentoRESUMO
BACKGROUND: The treatment of vitiligo is still one of the most difficult dermatological challenges, although there are many therapeutic options. Narrow band ultraviolet B (NB-UVB) phototherapy is considered to be a very important modality for generalized vitiligo. OBJECTIVE: The aim of this study was to explore whether a combination of NB-UVB and topical agents would be superior to NB-UVB alone for treating vitiligo. METHODS: We searched the electronic databases such as PUBMED, EMBASE, Cochrane Library, and Web of Science. The primary outcome was the proportion of ≥50% repigmentation (a clinical significance), and secondary outcome was the proportion of ≥75% repigmentation (an excellent response). RESULTS: Seven randomized controlled trials (RCTs) involving 240 patients (413 lesions) were included in this meta-analysis. The study showed no significant difference between NB-UVB combination therapy (NB-UVB and topical calcineurin inhibitor or vitamin D analogs) and NB-UVB monotherapy in the outcomes of ≥50% repigmentation and ≥75% repigmentation. However, lesions located on the face and neck had better results in ≥50% repigmentation (RR = 1.40, 95% CI 1.08-1.81) and ≥75% repigmentation (RR = 1.88, 95% CI 1.10-3.20) with NB-UVB and topical calcineurin inhibitor combination therapy vs. NB-UVB monotherapy. CONCLUSIONS: The meta-analysis suggested that adding neither topical calcineurin inhibitors nor topical vitamin-D3 analogs on NB-UVB can yield significantly superior outcomes than NB-UVB monotherapy for treatment of vitiligo. However, addition of topical calcineurin inhibitors to NB-UVB may increase treatment outcomes in vitiligo affecting face and neck.
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Inibidores de Calcineurina/administração & dosagem , Terapia Ultravioleta/métodos , Vitamina D/análogos & derivados , Vitiligo/tratamento farmacológico , Vitiligo/radioterapia , Administração Cutânea , Calcitriol/administração & dosagem , Calcitriol/análogos & derivados , Quimiorradioterapia , Fármacos Dermatológicos/administração & dosagem , Di-Hidroxicolecalciferóis/administração & dosagem , Dermatoses Faciais/tratamento farmacológico , Dermatoses Faciais/radioterapia , Humanos , Pescoço , Ensaios Clínicos Controlados Aleatórios como Assunto , Pigmentação da Pele/efeitos dos fármacos , Pigmentação da Pele/efeitos da radiação , Tacrolimo/administração & dosagem , Tacrolimo/análogos & derivadosRESUMO
The immunostimulatory role of dietary saponins on swimming crabs was investigated under field conditions. Portunus trituberculatus were fed diets enriched with Quillaja saponin (QS) at 150, 300 and 450 mg kg-1. They had an enhanced growth rate and increased resistance against Vibrio alginolyticus compared to crabs not fed with QS. Significant effects were observed on the average body weight, percentage weight gain and specific growth rate (p < 0.05). Total hemocyte and hyaline cell counts of P. trituberculatus fed with 300 and 450 mg kg-1 saponin in their diets significantly increased (p < 0.05) compared to the control. Phenoloxidase, superoxide dismutase, catalase and glutathione peroxidase activities significantly increased in response to the incorporation of QS in the diet. However, the respiratory burst activity did not increase significantly. The phagocytic activity was significantly enhanced at 300 and 450 mg kg-1 of saponin. QS diets can enhance growth of P. trituberculatus and its immune resistance against V. alginolyticus. Dietary administration of saponin may help to control diseases and improve production in the crab aquaculture industry.
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Braquiúros , Dieta , Suplementos Nutricionais , Imunidade Inata , Saponinas de Quilaia/imunologia , Vibrio alginolyticus/fisiologia , Ração Animal/análise , Animais , Braquiúros/crescimento & desenvolvimento , Braquiúros/imunologia , Braquiúros/microbiologiaRESUMO
This study aimed to evaluate the role of different amount of zeolite with low dosage of lime amendment on the greenhouse gas (GHGs) emission and maturity during the dewatered fresh sewage sludge (DFSS) composting. The evolution of CO2, CH4, NH3 and N2O and maturity indexes were monitored in five composting mixtures prepared from DFSS mixed with wheat straw, while 10%, 15% and 30% zeolite+1% lime were supplemented (dry weight basis of DFSS) into the composting mass and compared with treatment only 1% lime amended and control without any amendment. The results showed that addition of higher dosage of zeolite+1% lime drastically reduce the GHGs emissions and NH3 loss. Comparison of GHGs emissions and compost quality showed that zeolite amended treatments were superior than control and 1% lime amended treatments. Therefore, DFSS composting with 30% zeolite+1% lime as consortium of additives were found to emit very less amount of GHGs and gave the highest maturity than other treatments.
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Compostos de Cálcio/química , Gases/análise , Óxidos/química , Esgotos , Solo , Zeolitas/química , Amônia/análise , Dióxido de Carbono/análise , Efeito Estufa , Concentração de Íons de Hidrogênio , Metano/análise , Óxido Nitroso/análise , Esgotos/química , Eliminação de Resíduos Líquidos/métodosRESUMO
BACKGROUND AND AIMS: Ferns are abundant in sub-tropical forests in southern China, with some species being restricted to shaded understorey of natural forests, while others are widespread in disturbed, open habitats. To explain this distribution pattern, we hypothesize that ferns that occur in disturbed forests (FDF) have a different leaf cost-benefit strategy compared with ferns that occur in natural forests (FNF), with a quicker return on carbon investment in disturbed habitats compared with old-growth forests. METHODS: We chose 16 fern species from contrasting light habitats (eight FDF and eight FNF) and studied leaf functional traits, including leaf life span (LLS), specific leaf area (SLA), leaf nitrogen and phosphorus concentrations (N and P), maximum net photosynthetic rates (A), leaf construction cost (CC) and payback time (PBT), to conduct a leaf cost-benefit analysis for the two fern groups. KEY RESULTS: The two groups, FDF and FNF, did not differ significantly in SLA, leaf N and P, and CC, but FDF had significantly higher A, greater photosynthetic nitrogen- and phosphorus-use efficiencies (PNUE and PPUE), and shorter PBT and LLS compared with FNF. Further, across the 16 fern species, LLS was significantly correlated with A, PNUE, PPUE and PBT, but not with SLA and CC. CONCLUSIONS: Our results demonstrate that leaf cost-benefit analysis contributes to understanding the distribution pattern of ferns in contrasting light habitats of sub-tropical forests: FDF employing a quick-return strategy can pre-empt resources and rapidly grow in the high-resource environment of open habitats; while a slow-return strategy in FNF allows their persistence in the shaded understorey of old-growth forests.
Assuntos
Ecossistema , Gleiquênias/efeitos da radiação , Florestas , Luz , Folhas de Planta/efeitos da radiação , Clima Tropical , Dióxido de Carbono/farmacologia , Respiração Celular/efeitos dos fármacos , Respiração Celular/efeitos da radiação , Nitrogênio/metabolismo , Fósforo/metabolismo , Fotossíntese/efeitos dos fármacos , Fotossíntese/efeitos da radiação , Folhas de Planta/efeitos dos fármacos , Análise de Componente Principal , Característica Quantitativa HerdávelRESUMO
It is important to understand the ecophysiological characters of plants when exploring mechanisms underlying species substitution in the process of plant succession. In the present study, we selected 34 woody species from different stages of secondary succession in subtropical forests of southern China, and measured their hydraulic conductivity, gas exchange rates, leaf nutrients and drought-tolerance traits such as xylem resistance to cavitation, turgor loss point and carbon isotope ratio. Principal component analysis revealed that early-, mid- and late-successional species were significantly separated along axis 1, which was strongly associated with hydraulic-photosynthetic coordination. In contrast to species distributed in late-successional forest, early-successional species had the highest hydraulic conductivity, net photosynthetic rates, photosynthetic nitrogen and phosphorus use efficiencies, but had the lowest photosynthetic water-use efficiency. However, changes of the measured drought-tolerance traits of the 34 species along the succession did not demonstrate a clear trend - no significant correlations between these traits and plant successional stages were found. Moreover, the trade-off between hydraulic efficiency and safety was not identified. Taken together, our results suggested that hydraulic efficiency and photosynthetic function, rather than drought tolerance, play an important role in species distributions along plant succession in subtropical forests.