RESUMO
Essential oils (EOs) are liquid extracts derived from various parts of herbal or medicinal plants. They are widely accepted in food packaging due to their bioactive components, which exhibit remarkable antioxidant and antimicrobial properties against various pathogenic and food spoilage microorganisms. However, the functional efficacy of EOs is hindered by the high volatility of their bioactive compounds, leading to rapid release. Combining biopolymers with EOs forms a complex network within the polymeric matrix, reducing the volatility of EOs, controlling their release, and enhancing thermal and mechanical stability, favoring their application in food packaging or processing industries. This study presents a comprehensive overview of techniques used to encapsulate EOs, the natural polymers employed to load EOs, and the functional properties of EOs-loaded biopolymeric particles, along with their potential antioxidant and antimicrobial benefits. Additionally, a thorough discussion is provided on the widespread application of EOs-loaded biopolymers in the food industries. However, research on their utilization in confectionery processing, such as biscuits, chocolates, and others, remains limited. Further studies can be conducted to explore and expand the applications of EOs-loaded biopolymeric particles in food processing industries.
Assuntos
Anti-Infecciosos , Óleos Voláteis , Óleos Voláteis/farmacologia , Antioxidantes/farmacologia , Indústria de Processamento de Alimentos , Embalagem de Alimentos/métodos , Biopolímeros , Polímeros , Indústria AlimentíciaRESUMO
Rhizosphere is a soil volume of high spatio-temporal heterogeneity and intensive plant-soil-microbial interactions, for which visualization and process quantification is of highest scientific and applied relevance, but still very challenging. A novel methodology for quick assessment of two-dimensional distribution of available phosphorus (P) in rhizosphere was suggested, tested, and development up to the application platform. Available P was firstly trapped by an in-situ diffusive gradients in thin-films (DGT) sampler with precipitated zirconia as the binding gel, and subsequently, the loaded gel was analyzed with an optimized colorimetric imaging densitometry (CID). The imaging platform was established linking: i) DGT, ii) planar optode, and iii) soil zymography techniques to simultaneously determine available P, oxygen, and acid phosphatase in rhizosphere at sub-millimeter spatial scales. The DGT identified available P level in rice rhizosphere were spatially overlapping to the localized redox hotspots and phosphatase activity. The spatial relationship between available P and acid phosphatase activity was dependent on root development. The root radial oxygen loss (ROL) remained active during the experimental observations (2-3 days), while a flux of available P of 10 pg cm-2 s-1 was visualized within 2-3 mm of roots, confirming the correlative response of rice roots to oxygen secretion and P uptake. Summarizing, the established imaging platform is suitable to capture spatial heterogeneity and temporal dynamics of root activities, nutrient bioavailability, ROL and enzyme activities in rhizosphere.
Assuntos
Oryza , Fósforo , Fósforo/metabolismo , Rizosfera , Solo , Oxigênio/metabolismo , Fosfatase Ácida/metabolismo , Raízes de Plantas/metabolismoRESUMO
In this study, chitosan(CS), nano-silicon aerogels(nSA) and tea polyphenols(TP) were used as film-forming materials and processed with ultrasonication to form films using the tape-casting method. The effects of ultrasonication time, temperature and frequency on the properties of CS/nSA/TP film were explored via material property testing. The results of response surface showed that the maximum tensile strength of the film was 4.036â¯MPa at ultrasonication time(57.97â¯min), temperature(37.26 °C) and frequency(30â¯kHz). The maximum elongation at break of the film was 279.42 % at ultrasonication time(60.88â¯min), temperature(39.93 °C) and frequency(30â¯kHz). Due to cavitation and super-mixing effects, ultrasonication may make the surface of the film smoother and easier to degrade. After ultrasonication, TPs were protected by the 3D network structure composed of CS and nSA. Ultrasonication improved the antioxidant and antibacterial properties of the film. These results show that ultrasonication is an effective method to improve the properties of films.
Assuntos
Quitosana , Quitosana/química , Embalagem de Alimentos/métodos , Polifenóis/química , Dióxido de Silício , Chá , Resistência à TraçãoRESUMO
In this study, chitosan/halloysite nanotubes/tea polyphenol (CS/HNTs/TP) nanocomposite films were prepared by the solution casting method. The scanning electron microscopy (SEM) result showed that the nanocomposite film with a CS/HNTs ratio of 6:4 and a TP content of 10% (C6H4-TP10) had a relatively smooth surface and a dense internal structure. The water vapor barrier property of the nanocomposite film was improved due to the tortuous channels formed by the HNTs. However, the swelling degree and water solubility of the nanocomposite films were decreased. The nanocomposite films have a good antioxidant capacity. Antibacterial experiments showed that the C6H4-TP10 nanocomposite film had certain inhibitory effects on the growth of both E. coli and S. aureus. In addition, we used 3D printer to verify the printability of the optimal formulation of the film-forming solution. Overall, this strategy provides a simple approach to construct promising natural antioxidants and antibacterial food packaging.
Assuntos
Quitosana/análogos & derivados , Argila/química , Nanocompostos/química , Nanotubos/química , Polifenóis/química , Impressão Tridimensional , Antibacterianos/química , Antibacterianos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Camellia sinensis/química , Escherichia coli/efeitos dos fármacos , Staphylococcus aureus/efeitos dos fármacosRESUMO
The present study aims to investigate the effects of Phragmites communis and Zizania aquatica on rhizosphere soil enzyme activity and bacterial community structure in a surface flow constructed wetland (SFCW) for the treatment of domestic sewage from the Shanxi province of China. The basic physical and chemical properties of the soil, the contents of soil urease (UE), alkaline phosphatase (ALP), soil microbial biomass carbon and nitrogen (SMBC, SMBN), and bacterial community structure were measured in the Phragmites communis group (PG), Zizania aquatica group (ZG), and control group (CG), respectively. The results showed that (1) the contents of UE, ALP, SMBC, and SMBN in rhizosphere soil of PG were more than those of ZG; (2) the highest bacterial abundance and α-diversity appeared in PG, in which Gp6 was the most abundant bacterial genus in PG; (3) the main functions of the dominant bacteria Gp6 and Longilinea in PG were involved in metabolizing multiple carbohydrates and participating in the carbon cycle in the soil based on the clusters of orthologous groups pathway analysis data; (4) the bacterial community of PG was mainly affected by the positive correlation with arsenic, nickel, or SMBC via the redundancy analysis. Collectively, Phragmites communis is a recommended species for wastewater wetland treatment system in Shanxi province, and the special enzymes and dominant bacteria in plant rhizosphere soil had obvious functions of removing organic pollutants. Besides, the influences of environmental factors on rhizosphere bacteria and the combined effects of Phragmites communis and dominant bacteria in wetland wastewater treatment system should be taken seriously.
Assuntos
Rizosfera , Áreas Alagadas , China , Solo , Microbiologia do SoloRESUMO
Anoectochilus roxburghii, a traditional Chinese medicinal herb, has been widely used for treating numerous chronic diseases. In this study, a polysaccharide from A. roxburghii (ARPs-p) was purified by anion exchange and size exclusion chromatography. The structural characteristics of ARPs-p were systematically investigated for the first time via numerous chromatographic techniques, periodic acid oxidation, Smith degradation, methylation analysis, FTIR spectroscopy and 1D/2D NMR spectroscopy. The results showed that ARPs-p is a heteropolysaccharide with a molecular weight of 97 kDa; it consists of 97.75% glucose, 1.2% galactose and trace amounts of galacturonic acid, and its backbone structure is composed of â3)-ß-d-Glcp-(1â with some branching points at O-6 linked to non-reducing end units or 6-O-linked Glcp units. Furthermore, streptozotocin (STZ)-induced diabetic mouse experiments suggested that ARPs-p has excellent antihyperglycemic, antioxidant and antihyperlipidemic activities, in which 1,3-ß-d-glucan, the main component of ARPs-p, plays a vital role.
Assuntos
Hipoglicemiantes/química , Hipoglicemiantes/isolamento & purificação , Orchidaceae/química , Polissacarídeos/química , Polissacarídeos/isolamento & purificação , Animais , Antioxidantes , Diabetes Mellitus Experimental , Galactose , Glucose/química , Ácidos Hexurônicos , Hipoglicemiantes/farmacologia , Camundongos , Peso Molecular , Polissacarídeos/farmacologia , Espectroscopia de Infravermelho com Transformada de Fourier , Estreptozocina/efeitos adversosRESUMO
In order to explore Cassia seed polysaccharides (CSPs) as natural antioxidants for application in the functional-food industry, microwave-assisted extraction (MAE) was optimized for the extraction of CSPs by using a response surface methodology. Furthermore, the chemical structures and antioxidant activities of CSPs extracted by MAE and hot water extraction were investigated and compared. The maximum extraction yield of CSPs extracted by MAE (8.02 ± 0.19%) was obtained at the optimized extraction parameters as follows: microwave power (415 W), extraction time (7.0 min), and ratio of water to raw material (51 mL/g). Additionally, the contents of the uronic acids, molecular weight, ratio of constituent monosaccharides, intrinsic viscosities, and degrees of esterification of CSPs were significantly affected by the MAE method. Moreover, CSPs exhibited remarkable 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) ABTS, 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl DPPH, nitric oxide, and hydroxyl radical scavenging activities as well as reducing power. The high antioxidant activities observed in CSPs extracted by MAE could be partially attributed to its low molecular weights and high content of unmethylated galacturonic acid. Results indicate that the MAE method could be an efficient technique for the extraction of CSPs with high antioxidant activity, and CSPs could be further explored as functional food ingredients.