["Double Grooves-Double Rings" technique of transurethral Thulium laser enucleation of the prostate: learning curve of single surgeon].

Objective: To evaluate the learning curve of the "Double Grooves-Double Rings" (DGDR) technique of transurethral Thulium laser enucleation of the prostate (ThuLEP) for benign prostatic hyperplasia (BPH) by a single surgeon. From June 2021 to July 2022, 84 patients mean age (69.0±8.0) years,preoperative prostate volume (90.9±40.3)ml with BPH underwent ThuLEP in the Department of Urology, Peking University First Hospital.Performed by a single surgeon who had no experience of transurethral resection of prostate (TURP) and any laser surgeries. The case scatter plots with the best fitting line were drawn to analyze the learning curve. According to the date of the surgeries, the patients were equally divided into three learning stages (28 patients for each group). The T-PSA,prostate volume,operative time,enucleation time, enucleation efficiency,catheter indwelling time, hemoglobin drop and perioperative complications (including re-TURP, blood transfusion, stress incontinence≥3 months and urethral stricture) were compared among the groups. The learning curve was divided into three stages, and the cutting point was shown on the 14th case. Except the prostate volume [stage1 (75.7±30.7) ml, stage2 (93.40±39.6)ml, stage3 (103.5±46.2) ml, P<0.05], there was no significant difference of the baseline data between three groups (P>0.05). Compared with those of stage 1(100.6±24.7) min,(0.55±0.22) g/min, a statistically significant improvement was observed in both of the operative time and the enucleation efficiency among stage 2[(84.5±36.6) min, (0.87±0.33) g/min and stage 3 (71.2±26.3) min, (1.27±0.45) g/min, P<0.05]. The learning curve of the DGDR technique for ThuLEP can be divided into three stages. A ThuLEP beginner can preliminarily master this technique after completing 14 cases.

Effects of lipid form and source on digestibility of fat and fatty acids in growing pigs.

The objective of this study was to compare the effect of source (corn DDGS, rice bran, or soybean) or form of oil (extracted or intact) on apparent total tract digestibility (ATTD) and true total tract digestibility (TTTD) of acid-hydrolyzed ether extract (AEE) and fatty acids. The study determined and compared the ATTD or TTTD of AEE and fatty acids in extracted corn oil, rice oil, and soybean oil with intact oil in corn DDGS, full-fat rice bran, and full-fat soybean. Seventy-eight barrows (initial BW = 47.2 ± 3.9 kg; Duroc × Landrace × Yorkshire) were allotted to 1 of 13 dietary treatments in a randomized complete block design with 6 barrows in each dietary treatment. The 13 experimental diets included 1 cornstarch-soybean meal basal diet (AEE, 0.56%) and 3 diets containing 6% extracted oils (corn oil, rice oil, and soybean oil) and 9 diets supplemented with 3 levels of corn DDGS (17%, 34%, and 51%), full-fat rice bran (14%, 28%, and 42%), and full-fat soybean (12%, 24%, and 36%). These diets provided about 2%, 4%, and 6% intact oil, respectively. The barrows were housed in individual metabolism crates and were fed the assigned test diets at 4% of initial BW per day. A 5-d total collection of feces followed a 7-d diet adaptation period. The ATTD of AEE were calculated for each diet. The endogenous flow of AEE associated with each ingredient and values for TTTD were calculated using regression methods. The ATTD of AEE were greater ( < 0.05) for extracted oil than for intact oil. Compared to extracted oil, intact oil had lower ( < 0.01) ATTD of palmitic acid (C16:0), stearic acid (C18:0), oleic acid (C18:1), linoleic acid (C18:2), and linolenic acid (C18:3). However, a source by form interaction ( < 0.01) was observed for ATTD of C18:0, C18:2, and C18:3. The ATTD of C18:0, C18:2, and C18:3 in extracted corn and rice oil were greater compared with intact corn DDGS and rice oil ( < 0.05), while there was no significant difference for the ATTD of C18:0, C18:2, and C18:3 between extracted soybean oil and intact oil in full-fat soybean. In conclusion, lipids that are extracted have a greater digestibility compared with intact lipids, and this is especially true regarding saturated fatty acids. The ATTD of AEE in 2 forms of rice oil (intact oil and extracted oil) was less than the values in corn oil and soybean oil. The TTTD of AEE in corn DDGS and full-fat soybeans were greater than in full-fat rice bran.

Determination and prediction of energy values in corn distillers dried grains with solubles sources with varying oil content for growing pigs.

This study was conducted to determine the DE and ME content of 25 samples of corn distillers dried grains with solubles (DDGS) fed to growing pigs and to generate prediction equations for DE and ME based on chemical analysis. The 25 samples included 15 full-oil (no oil extracted; ether extract [EE] > 8%) DDGS and 10 reduced-oil (oil extracted; EE < 8%) DDGS collected from 17 ethanol plants in China. A corn­soybean meal diet constituted the basal diet and the other 25 diets replaced a portion of the corn, soybean meal, and lysine of the basal diet with 28.8% of 1 of the 25 corn DDGS sources. Seventy-eight barrows (initial BW = 42.6 ± 6.2 kg) were used in the experiment conducted over 2 consecutive periods (n = 6 per treatment) using a completely randomized design. For each period, pigs were placed in metabolism cages for a 5-d total collection of feces and urine following a 7-d adaptation to the diets. Among the 25 corn DDGS samples, EE, NDF, DE, and ME content (DM basis) ranged from 2.8 to 14.2%, 31.0 to 46.6%, 3,255 to 4,103 kcal/kg, and 2,955 to 3,899 kcal/kg, respectively. Using a stepwise regression analysis, a series of DE and ME prediction equations were developed not only among all 25 DDGS but also only within 15 full-oil DDGS and 10 reduced-oil DDGS samples. The best fit equations of DE (kcal/kg DM) for the complete set of 25 DDGS, 15 full-oil DDGS, and 10 reduced-oil DDGS were 2,064 ­ (38.51 × % NDF) + (0.64 × % GE) ­ (39.70 × % ash), ­(87.53 × % ADF) + (1.02 × % GE) ­ (22.99 × % hemicellulose), and 3,491 ­ (40.25 × % NDF) + (46.95 × % CP), respectively. The best fit equations for ME (kcal/kg DM) for the complete set of 25 DDGS, 15 full-oil DDGS, and 10 reduced-oil DDGS were 1,554 ­ (44.11 × % NDF) + (0.77 × % GE) ­ (68.51 × % ash), 7,898 ­ (42.08 × % NDF) ­ (136.17 × % ash) + (101.19 × % EE) (103.83 × % CP), and 4,066 ­ (46.30 × % NDF) + (45.80 × % CP) ­ (106.19 × % ash), respectively. Using the sum of squared residuals to compare the accuracy of the 3 groups of prediction equations revealed that separate equations for full-oil DDGS and reduced-oil DDGS each provided a better fit than a single equation for the entire set of DDGS sources. These results indicated that the DE and ME values in corn DDGS are related to the chemical composition, primarily the EE and fiber concentrations. Specific prediction equations derived from full-oil and reduced-oil DDGS are better than equations derived from the entire set of DDGS.

[Effect of allitridi on expression of proliferating cell nuclear antigen of cultured rabbit aortic smooth muscle cell].

OBJECTIVE: To observe the effect of allitridi on the expression of proliferating cell nuclear antigen (PCNA) of cultured rabbit aortic smooth muscle cell (SMC). METHODS: The expression of PCNA was examined with immunohistochemical technique (LSAB method) and the contents of superoxide dismutase (SOD), lipid peroxide (LPO), prostacyclin (PGI2) and cyclic adenosine monophosphate (cAMP) in medium were simultaneously determined. RESULTS: Allitridi has the effects of increasing SOD activity, decreasing LPO, elevating PGI2 and cAMP, reducing the expression of PCNA (all P < 0.05-0.01). CONCLUSION: Allitridi could inhibit SMC proliferation.

[A study on the retreatment of pulmonary tuberculosis by the addition of 654-2].

654-2 plus chemotherapy were used to retreat TB. After a full course of treatment, rates of negative sputum in the observation group and in the control group were 90.6% and 68.8% respectively (The difference in the rates was statistically significant P less than 0.05). The total rate of focus in the observation group was 46.9% and that in the control group was 21.9%. The difference was striking (P less than 0.05). The general efficacy for cavity alteration in the observation group was 42.3% and in control group was 16.0%. Comparing the two groups, there was an obvious difference (P less than 0.05). After ceasing medication, no relapse was observed through a period of one year in the treated group.