RESUMO
Eicosapentaenoic acid (EPA), an omega-3 fatty acid, has been widely used to prevent cardiovascular disease (CVD) and treat brain diseases alone or in combination with docosahexaenoic acid (DHA). However, the impact of EPA and DHA supplementation on normal cognitive function and the molecular targets of EPA and DHA are still unknown. We show that acute administration of EPA impairs learning and memory and hippocampal LTP in adult and prepubescent mice. Similar deficits are duplicated by endogenously elevating EPA in the hippocampus in the transgenic fat-1 mouse. Furthermore, the damaging effects of EPA are mediated through enhancing GABAergic transmission via the 5-HT6R. Interestingly, DHA can prevent EPA-induced impairments at a ratio of EPA to DHA similar to that in marine fish oil via the 5-HT2CR. We conclude that EPA exhibits an unexpected detrimental impact on cognitive functions, suggesting that caution must be exercised in omega-3 fatty acid supplementation and the combination of EPA and DHA at a natural ratio is critical for learning and memory and synaptic plasticity.
Assuntos
Cognição/efeitos dos fármacos , Ácido Eicosapentaenoico/efeitos adversos , Neurônios GABAérgicos/efeitos dos fármacos , Receptor 5-HT2C de Serotonina/efeitos dos fármacos , Animais , Suplementos Nutricionais/efeitos adversos , Ácidos Docosa-Hexaenoicos/farmacologia , Combinação de Medicamentos , Ácido Eicosapentaenoico/farmacologia , Ácidos Graxos Ômega-3/efeitos adversos , Óleos de Peixe/efeitos adversos , Óleos de Peixe/farmacologia , Humanos , Aprendizagem/efeitos dos fármacos , Transtornos da Memória/etiologia , Transtornos da Memória/patologia , CamundongosRESUMO
AIDS caused by HIV-1, is a major threat to human being. An anti-HIV formulation from Chinese herbs, so called "Qu Du Zeng Ning", have been recently developed. In this work, the pharmacodynamics of the formulation in vitro was studied. The results showed that Qu Du Zeng Ning inhibit the replication of HIV-1 efficiently in all cell-based assay, with IC50 at 105.2, 70.7, 77.4 microg mL(-1), separately. A significant synergy between the formulation and zidovudine (AZT) was observed, and it also showed a potent activity against HIV-1 drug-resistant mutant.
Assuntos
Fármacos Anti-HIV/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , HIV-1/fisiologia , Replicação Viral/efeitos dos fármacos , Fármacos Anti-HIV/isolamento & purificação , Células Cultivadas/virologia , Combinação de Medicamentos , Farmacorresistência Viral , Sinergismo Farmacológico , Medicamentos de Ervas Chinesas/isolamento & purificação , Inibidores da Protease de HIV/farmacologia , HIV-1/efeitos dos fármacos , Humanos , Concentração Inibidora 50 , Plantas Medicinais/química , Scutellaria/química , Zidovudina/farmacologiaRESUMO
OBJECTIVE: To study the effect of anti-HIV III B virus with extractions from Juglans regia, so as to searching for the new and efficacious leading compound of AIDS therapy. METHOD: Phytochemical and chromatographical techniques were used to isolate compounds from J. regia; MT4 cells and HIV III B virus were used to study the effect of anti-HIV activity in vitro. BIACORE 3000 molecule coupled equipment were used for the target research also. RESULT: Two extractions (B&E) were isolated from J. regia which possess the effect of anti-HIV activity. Targets study found that extraction B could affected on HIV-1 gp-41 fusing protein and extraction E could affected on HIV-1 integrase respectively. CONCLUSION: J. regia is a traditional Chinese medicine with active anti-HIV activity and worth to be studied further.
Assuntos
Medicamentos de Ervas Chinesas/farmacologia , HIV-1/efeitos dos fármacos , Juglans/química , Extratos Vegetais/farmacologia , Linhagem Celular , Medicamentos de Ervas Chinesas/química , Humanos , Extratos Vegetais/químicaRESUMO
OBJECTIVE: To probe into the feasibility of screening anti-HIV compounds by using HIV-1 p24 detection kit made by Hebei Medical University. METHODS: The sensitivity, reproducibility and efficacy of the Hebei p24 kit were evaluated compared with the commercially available Vironostika HIV-1 Antigen Microelisa System (Biomerieux). RESULTS: Hebei p24 kit had high sensitivity and good reproducibility. In vitro screening demonstrated that there was no statistically significant difference (P greater than 0.05) between these two kits in assessing anti-HIV compounds. CONCLUSION: Hebei p24 kit could be used as an easily affordable alternative method for detection of HIV-1 in screening anti-HIV compounds.
Assuntos
Fármacos Anti-HIV/farmacologia , Proteína do Núcleo p24 do HIV/análise , HIV-1/efeitos dos fármacos , Kit de Reagentes para Diagnóstico/normas , Fármacos Anti-HIV/isolamento & purificação , Linhagem Celular , Avaliação Pré-Clínica de Medicamentos/instrumentação , Avaliação Pré-Clínica de Medicamentos/métodos , Estudos de Viabilidade , HIV-1/crescimento & desenvolvimento , HIV-1/imunologia , Humanos , Reprodutibilidade dos TestesRESUMO
OBJECTIVE: To evaluate the therapeutic effect of QuDu ZengNing Capsule on AIDS. METHODS: QuDu ZengNing Capsule is a capsule containing extract from 4 Chinese medicinal herbs. Totally 1,000 AIDS patients were treated, among them 60 patients were clinically observed weekly. Blood routine tests, liver, heart and kidney function, X-ray, CD4, CD8 cells were examined before and after treatment at 1, 3, 6 month. The patients were treated with 4 capsules t.i.d for 6 months. RESULTS: The symptoms were improved in most of the patients, the CD4 cells increased from 115.0 to 295.2/ul and the viral load (RNA copies/ml) in most patients reduced markedly or maintained at the same level. CONCLUSION: These data indicated that QuDu ZengNing Capsule was effective for treatment of AIDS patients.