[Quality standards of Gleditsiae Spina and its decoction pieces].

In view of the current inadequate standards for Gleditsiae Spina in the Chinese Pharmacopoeia, this study put forward some new items of the quality standards of Gleditsiae Spina. Thin-layer chromatography(TLC) was performed for identification with the reference substance of taxifolin and the reference material of Gleditsiae Spina as the control. According to the general principles of the Chinese Pharmacopoeia(2020 edition, Vol. 4), the moisture, total ash content, and alcohol-soluble extract of medicinal materials and decoction pieces of Gleditsiae Spina were determined. The content determination method for medicinal materials and decoction pieces of Gleditsiae Spina was established using high-performance liquid chromatography(HPLC), with taxifolin as the quality control index. Based on the determination results of 30 batches of samples of Gleditsiae Spina from different habitats, the draft quality standards of Gleditsiae Spina were developed, which provided suggestions for the revision of the quality standards of Gleditsiae Spina in the Chinese Pharmacopoeia.

Neuroprotective effects of Yiqihuoxue calm wind capsule on ischemic stroke in rats.

Stroke remains the third leading cause of death and of adult disability worldwide. Vascular occlusion, followed by ischemic cascade, leads to irreversible tissue injury. Recombinant tissue plasminogen activator is the only FDA approved drug for the current treatment of acute ischemic stroke. However, traditional Chinese medicine has a long history and rich clinical experience in the treatment and rehabilitation of ischemic stroke. Using a classical middle cerebral artery occlusion (MCAO) stroke model, we tested the effectiveness of Yiqihuoxue calm wind (YCW) capsule on neurological function, gross pathology and oxidative stress status in MCAO rats. YCW capsule (3.36 and 6.72 g·kg-1 of crude drug) could significantly lower Longa's score and superoxide dismutase (SOD) level, together with less necrotic cells and infarcted area. In addition to elevated MDA and downregulated iNOS expression, YCW capsule exhibited its neuroprotective effects via free radical scavenging and NO inhibition.

[Chemical constituents from lipophilic parts in roots of Angelica dahurica cv.Yubaizhi].

The chemical constituents from lipophilic parts in the roots of Angelica dahurica cv. Yubaizhi were studied in this paper. The compounds were separated and purified by repeated column chromatographic methods on silica gel and HPLC, and the chemical structures of compounds were determined by spectral data analyses. Thirty-three compounds were obtained and identified as isoimperatorin (1), imperatorin (2), stigmasterol (3), isooxypeucedanin (4), pabulenol (5), psoralen (6), bergapten (7), isodemethylfuropinarine (8), phellopterin (9), osthenol (10), alloimperatorin (11), xanthotoxin (12), xanthotoxol (13), isopimpinellin (14), alloisoimperatorin (15), ß-sitosterol (16), oxyalloimperatorin (17), pabularinone (18), 5-hydroxy-8-methoxypsoralen (19), columbianetin (20), heracol (21), isogosferol (22), 2″R-neobyakangelicol (23), byakangelicin ethoxide (24), byakangelicin (25), oxypeucedanin hydrate (26), uracil (27), umbelliferone (28), bergaptol (29), demethylfuropinarine (30), isobyakangelicol (31), oxypeucedanin ethanolate (32), heraclenol (33). Among them, compounds 8, 10, 17, 21, and 30 were obtained from the roots of title plant for the first time.

[Effects on phosphorus fraction distribution in sediment by roots of Vallisneria natans].

The present study explored phosphorus fractions in sediments with the growth of Vallisneria natans. Sediment samples in different layers were collected at 20, 50 and 80 d, and vertical change of several phosphorus fractions were measured in the samples. The root distributions and biomass of the V. natans were measured. Our results showed that roots were distributed between 0 and 14 cm in the experimental device. The average number of roots and average root length were 58 and 5.86 cm. After 80 days growth, the percentage of V. natans root biomass were 45.99%, 32.75%, 16.03% and 5.23% in the sediment with depths of 0-3, 4-6, 7-10 and 11-14 cm. Total phosphorus (TP) content, phosphorus extracted by NaOH (NaOH-P), and organic phosphorus (OP) levels remarkably decreased (P < 0.05) in the area with a high concentration of tape grass roots. The content of phosphorus extracted by HCl (HCl-P), and inorganic phosphorus (IP), showed no significant difference (P > 0.05). The results suggest that V. natans root affects the migration and transformation of phosphorus species in the sediment.

[Influence of submerged macrophytes on phosphorus transference between sediment and overlying water in the growth period].

In order to study the process of phosphorus transfer between sediment and overlying water, Hydrilla verticillata and Vallisneria natans were cultured in spring, Potamogeton crispus was cultured in winter. Changes of environmental factors and phosphorus concentrations in water and sediment were investigated. The results indicated that: submerged macrophytes could reduce all phosphorus fractions in the overlying water. Phosphorus concentrations in overlying water maintained in a relative low level in the growth period of submerged macrophytes. The concentrations of total phosphorus (TP) in overlying water of H. verticillata, V. natans and P. crispus were 0.03-0.05, 0.04-0.12, 0.02-0.11 mg x L(-1), respectively. All phosphorus fractions in sediment were reduced. The maximum value between submerged macrophyte and control of H. verticillata, V. natans and P. crispus were 35.34, 60.67 and 25.92 mg x kg(-1), respectively. Dissolved oxygen (DO), redox potential (Eh) and pH in overlying water increased (DO 10.0-14.0 mg x L(-1), Eh 185-240 mV, pH 8.0-11.0) in the submerged macrophytes groups. Submerged macrophytes increased Eh( -140 - -23 mV) and maintained pH(7.2-8.0) in neutral range. The results indicated that submerged macrophytes affected phosphorus transferring between sediment and overlying water through increasing DO, Eh and pH in overlying water, and Eh in sediment.

[Constituents of essential oil from different processing drynesses of Radix Angelicae Dahuricae].

OBJECTIVE: To analyze the constituents of essential oil from different processing drynesses of Radix Angelicae Dahuricae. METHODS: Water steam distillation and GC-MS were used. Relative contents were determined by area. RESULTS: 37 compounds were identified. The constituents of essential oil the constituents from Radix Angelicae Dahuricae by insolation, drying and microwave dryness were similar, but one by dryness after sulfurizing and dryness after perspiring were different. CONCLUSION: Dryness after sulfurizing and dryness after perspiring are not fit for the dryness of Radix Angelicae Dahuricae.

Characterization of active phenolic components in the ethanolic extract of Ananas comosus L. leaves using high-performance liquid chromatography with diode array detection and tandem mass spectrometry.

HPLC-DAD-MS was utilized to investigate the phytochemical constituents in ethanolic extract of Ananas comosus L. leaves (EEACL) responsible for antidiabetic, antihyperlipidemic and antioxidative effects. Eight phenylpropane diglycerides, together with two hydroxycinnamic acids, three hydroxycinnamoyl quinic acids, four phenylpropane monoglycerides, three flavones and six phenylpropanoid glycosides were detected, and their proposed structures were elucidated based on HPLC retention time, UV and MS profiles. Meanwhile, a new HPLC-DAD-MS method was established for the identification and characterization of phenylpropane diglycerides in natural plants.

[Study on HPLC fingerprint of processed Radix Angelicae Dahuricae].

OBJECTIVE: To establish HPLC fingerprint analysis for the quality control of processed Radix Angelicae Dahuricae. METHODS: HPLC fingerprint analysis of processed Radix Angelicae Dahuricae was developed Agilent C18 column (250 mm x 4.6 mm, 5 microm) was used, with mixture of acetonitrile and 5% phosphorous acid mobile phase in a gradient mode. The flow rate was 1.0 mL/min. The wavelength of measurement was 254 nm. Fifteen batches of processed Radix Angelicae Dahuricae were determined. RESULTS: The methodological evaluation showed that the method had a good repeatability. CONCLUSION: The method can be used to identify and evaluate the quality of Radix Angelicae Dahuricae conveniently.