RESUMO
A new quinone compound, p-hydroxymethoxybenzobijuglone (HMBBJ), isolated from Juglans mandshurica by bioassay-guided fractionation, showed cytotoxic activity against HeLa cell line. Its chemical structure was determined by NMR and HREIMS spectra. In this paper, its ability to induce apoptosis in HeLa cells was studied for the first time. After treated with HMBBJ, the growth of HeLa cells was inhibited and cells displayed typical morphological apoptotic characteristics. Data from flow cytometry analysis showed that the HeLa cell cycle was arrested in the G2/M phase by HMBBJ, and the apoptotic rate of HeLa cells increased in a dose-dependent manner. Meanwhile, HMBBJ increased the expression of caspase-8, -3 and Bax, decreased the expression of Bcl-2, and lowered the DeltaPsi(m). These findings reveal that HMBBJ could efficiently induce HeLa cells apoptosis through mitochondria dependent pathway and activation of the caspase cascade, and it may be a potential chemotherapeutic candidate for the treatment of cancer.
Assuntos
Apoptose/efeitos dos fármacos , Benzoquinonas/farmacologia , Caspase 3/metabolismo , Caspase 8/metabolismo , Feminino , Citometria de Fluxo , Células HeLa , Humanos , Potenciais da Membrana/efeitos dos fármacos , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/fisiologia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Neoplasias do Colo do Útero/patologia , Proteína X Associada a bcl-2/metabolismoRESUMO
OBJECTIVE: To study the formulation and technique of preparation of rapid-dissoluted EGb (Extract of Ginkgo biloba) droppills. METHOD: Taking the dissolution percentage of total flavonoids in EGb and weight variation as index, the formulation and technique of EGb droppills were optimized by the orthogonal experiment. RESULT: T50 was 3.62 min and mean weight variation was 2.80%. CONCLUSION: Rapid-dissoluted EGb droppills can increase the dissoluting rate distinctly and reach the purpose of preparation.