RESUMO
Antrodia camphorata, a well-known and highly valued edible medicinal mushroom with intriguing activities like liver protection, has been traditionally used for the treatment of alcoholic liver disease. A. camphorata shows highly medicinal and commercial values with the demand far exceeds the available supply. Thus, the petri-dish cultured A. camphorata (PDCA) is expected to develope as a substitute. In this paper, nineteen triterpenes were isolated from PDCA, and thirteen of them were the unique anthroic acids in A. camphorata, including the main content antcin K, which suggested that PDCA produced a large array of the same anthroic acids as the wild one. Furthermore, no obvious acute toxicity was found suggesting the edible safety of PDCA. In mice alcohol-induced liver injury model, triglyceride (TG), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and malondialdehyde (MDA) had been reduced by the PDCA powder as well as the main content antcin K, which indicated that the PDCA could protect alcoholic liver injury in mice model and antcin K could be the effective component responsible for the hepatoprotective activities of PDCA against alcoholic liver diseases.
Assuntos
Antrodia/química , Produtos Biológicos/farmacologia , Produtos Biológicos/uso terapêutico , Doença Hepática Induzida por Substâncias e Drogas/prevenção & controle , Fígado/efeitos dos fármacos , Alanina Transaminase/sangue , Aldeído Desidrogenase/sangue , Animais , Aspartato Aminotransferases/sangue , Produtos Biológicos/química , Doença Hepática Induzida por Substâncias e Drogas/etiologia , Colestenos/química , Colestenos/farmacologia , Colestenos/uso terapêutico , VLDL-Colesterol/sangue , Modelos Animais de Doenças , Etanol/toxicidade , Feminino , Carpóforos/química , Fígado/metabolismo , Fígado/patologia , Hepatopatias Alcoólicas/prevenção & controle , Masculino , Malondialdeído/sangue , Camundongos , Estrutura Molecular , Triglicerídeos/sangue , Triterpenos/química , Triterpenos/farmacologia , Triterpenos/uso terapêuticoRESUMO
Cytoskyrin C (1), a new bisanthraquinone with asymmetrically cytoskyrin type skeleton, together with a known symmetrical analogue (+)-epicytoskyrin (2), were isolated from an endophytic fungus ARL-09 (Diaporthe sp.). Cytoskyrin C (1) featured an asymmetrically cage-like structural motif arising from the dimerization of anthraquinone monomers by three carboncarbon bonds 9a/3', 3/9a' and 1/1'. The structure and absolute configuration of compound 1 were determined by spectroscopic analyses, ECD calculation and exciton chirality methods. Moreover, a plausible biogenetic pathway of 1-2 was predicted. Their cytotoxicities against SMMC-7721 cell as well as effects on NF-κB signaling pathway were evaluated. 2009 Elsevier Ltd. All rights reserved.