Circulating trace elements: Comparison between early and late incubation in common eiders (Somateria mollissima) in the central Baltic Sea.

We analyzed body mass and a panel of 64 trace elements in blood from incubating common eiders (Somateria mollissima) in the central Baltic Sea during the breeding seasons of 2017 (n = 27) and 2018 (n = 23). Using a non-invasive approach, the same incubating eiders nesting on Christiansø, Denmark were sampled once on day 4 and day 24 of incubation to provide a comparison between the early and late stages of incubation. Blood concentrations of chemical elements were quantified using high-resolution inductively coupled plasma mass spectrometry (HR-ICP-MS). Cadmium and lead significantly increased over the course of the incubation period while body mass, barium, calcium, cerium, cesium, iron, magnesium, manganese, molybdenum, phosphorus, selenium, strontium, sulfur, uranium, and zinc all significantly decreased. Excluding lead, all trace elements were within expected ranges. Lead blood concentrations had a 4.7-fold increase from 2017 to 2018 indicating a potential health threat. However, internal interactions between trace elements must be considered when making comparisons to toxicological thresholds. Body mass and many essential elements showed significantly higher levels in 2017 than 2018, which could be an indication of limitations in preferred food availability or harsher fasting conditions. Additional sampling years are needed to further investigate if these results reflect yearly fluctuations or decreasing health within the Christiansø eider colony. There was little overlap in element blood concentrations and body mass between days of incubation, indicating these parameters are affected by the physiological processes of reproduction and incubation. We recommend continued biomonitoring and use of complete trace element analysis for the Christiansø eiders to further understand year-to-year variations within colonies. Further investigation into the spatial ecology of the colony is also needed to provide a more robust understanding of exposure and source identification of trace elements.

Trace element concentrations in feathers and blood of Northern goshawk (Accipiter gentilis) nestlings from Norway and Spain.

Information on trace element pollution in the terrestrial environment and its biota is limited compared to the marine environment. In the present study, we collected body feathers and blood of 37 Northern goshawk (Accipiter gentilis) nestlings from Tromsø (northern Norway), Trondheim (central Norway), and Murcia (southeastern Spain) to study regional exposure, hypothesizing the potential health risks of metals and other trace elements. Blood and body feathers were analyzed by a high resolution inductively coupled plasma mass spectrometer (HR-ICP-MS) for aluminum (Al), nickel (Ni), copper (Cu), zinc (Zn), arsenic (As), selenium (Se), cadmium (Cd), mercury (Hg) and lead (Pb). The influence of regional differences, urbanization and agricultural land usage in proximity to the nesting Northern goshawks was investigated using particular spatial analysis techniques. Most trace elements were detected below literature blood toxicity thresholds, except for elevated concentrations (mean ± SD µgml-1 ww) found for Zn (5.4 ± 1.5), Cd (0.00023 ± 0.0002), and Hg (0.021 ± 0.01). Corresponding mean concentrations in feathers (mean ± SD µgg-1 dw) were 82.0 ± 12.4, 0.0018 ± 0.002, and 0.26 ± 0.2 for Zn, Cd and Hg respectively. Multiple linear regressions indicated region was a significant factor influencing Al, Zn, Se and Hg feather concentrations. Blood Cd and Hg concentrations were significantly influenced by agricultural land cover. Urbanization did not have a significant impact on trace element concentrations in either blood or feathers. Overall metal and trace element levels do not indicate a high risk for toxic effects in the nestlings. Levels of Cd in Tromsø and Hg in Trondheim were however above sub-lethal toxic threshold levels. For holistic risk assessment purposes it is important that the concentrations found in the nestlings of this study indicate that terrestrial raptors are exposed to various trace elements.

Trace elements in native and transplanted Fontinalis antipyretica and Platyhypnidium riparioides from rivers polluted by uranium mining.

The past uranium/polymetallic mining activities in the Sudety (SW Poland) left abandoned mines, pits, and dumps of waste rocks with trace elements and radionuclides which may erode or leach out and create a potential risk for the aquatic ecosystem, among others. In the present work four rivers affected by effluents from such mines were selected to evaluate the application of aquatic mosses for the bioindication of 56 elements. Naturally growing F. antipyretica and P. riparioides were compared with transplanted samples of the same species. The results demonstrate serious pollution of the examined rivers, especially with As, Ba, Fe, Mn, Pb, Ti, U and Zn, reaching extremely high concentrations in native moss samples. In the most polluted rivers native F. antipyretica and P. riparioides samples showed significantly higher concentrations of As, Ba, Cu, Fe, La, Nd, Ni, Pb, U and Zn than corresponding transplanted samples, whereas at less polluted sites a reverse situation was sometimes observed. Transplanted moss moved from clean to extremely polluted rivers probably protects itself against the accumulation of toxic elements by reducing their uptake. Selection of native or transplanted F. antipyretica and P. riparioides depended on the pollution load.

Effect of diet, location and sampling year on bioaccumulation of mercury, selenium and cadmium in pelagic feeding seabirds in Svalbard.

Hepatic concentrations of mercury (Hg), selenium (Se) and cadmium (Cd) were determined in black-legged kittiwakes (Rissa tridactyla) and little auks (Alle alle) from two fjords in Svalbard (Kongsfjorden; 78°57'N, 12°12'E and Liefdefjorden; 79°37'N, 13°20'E). The inflow of Arctic and Atlantic water differs between the two fjords, potentially affecting element accumulation. Trophic positions (TP) were derived from stable nitrogen isotope ratios (δ(15)N), and stable carbon isotope ratios (δ(13)C) were assessed to evaluate the terrestrial influence on element accumulation. Mercury, Cd, TP and δ(13)C varied significantly between locations and years in both species. Trophic position and feeding habits explained Hg and Cd accumulation in kittiwakes, but not in little auks. Biomagnification of Hg and Cd were found in the food webs of both the Atlantic and the Arctic fjord, and no inter-fjord differences were detected. The δ(13)C were higher in the seabirds from Kongsfjorden than in Liefdefjorden, but this did not explain variations in element accumulation. Selenium concentrations were not influenced by Hg accumulation in kittiwakes, indicating baseline levels of Se in this species. In contrast, correlations between Hg and Se and lower Se:Hg ratios in little auks from Kongsfjorden than in Liefdefjorden indicate a more pronounced influence of Se-Hg complex formation in little auks feeding in Atlantic waters.

A comparative study of hepatic trace element levels in wild moose, roe deer, and reindeer from Norway.

Liver samples from 422 wild moose (Alces alces), 280 roe deer (Capreolus capreolus), and 73 reindeer (Rangifer tarandus) collected by hunters in various localities in Norway, 2002-2003, were analyzed for the essential trace elements cobalt, copper (Cu), manganese (Mn), molybdenum, selenium (Se), and zinc. Significant differences in hepatic concentrations among species were found for all elements except for Mn, and considerable individual and geographic variations were seen. Roe deer had statistically significant lower Se levels (median: 0.51 µg Se/g dry weight) than did moose (0.77 µg Se/g) and reindeer (0.85 µg Se/g). Moose from two coastal municipalities with high precipitation had considerably higher Se concentrations than those from the other localities studied. Seventy-nine roe deer (28%) and 36 moose (9%) had Se concentrations below that regarded as deficient in domestic ruminants. The Se status in roe deer was lower than previously reported in Europe. Moose had a significantly higher Cu (222 µg Cu/g dw) than did roe deer (112 µg Cu/g) and reindeer (105 µg Cu/g). The Cu status of moose and roe deer in Norway are among the highest reported in Europe. However, a suboptimal Se and Cu status was found in moose from Tvedestrand, a population which has suffered from a reduced condition and productivity. The variability in trace element status among hunted cervids, with no apparent signs of deficiency or toxicity, probably reflects adaptations in these wild ruminant species to cope with this. However, subtle clinical signs and lesions are difficult to detect and further research is needed.

Selenium moderates mercury toxicity in free-ranging freshwater fish.

Due to the extremely high affinity of selenium (Se) to mercury (Hg), Se sequesters Hg and reduces its biological availability in organisms. However the converse is also true. Hg sequesters Se, causing Hg to inhibit the formation of Se dependent enzymes while supplemental Se supports their continued synthesis. Hence, whether or not toxic effects accompany exposure to Hg depends upon the tissue Se:Hg molar ratio of the organism. The main objective of the present study was to investigate how levels of Hg and Se affected metallothionein (MT) induction in free-ranging brown trout, Salmo trutta, from Lake Mjøsa, Norway (a Se depauperate lake). MT is proposed as a sensitive biomarker of potential detrimental effects induced by metals such as Hg. Emphasis was addressed to elucidate if increased tissue Se:Hg molar ratios and Se levels affected the demands for MT in the trout. The Se:Hg molar ratio followed by tissue Se levels were most successful for assessing the relationship between metal exposure and MT levels in the trout. Thus, Hg in molar excess over Se was a stronger inducer of MT synthesis than tissue Hg levels in the trout, supporting the assumption that Se has a prominent protective effect against Hg toxicity. Measuring Hg in animals may therefore provide an inadequate reflection of the potential health risks to humans and wildlife if the protective effects of Se are not considered.