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3.
Am J Chin Med ; 49(3): 677-703, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33704029

RESUMO

To investigate the therapeutic efficacy of Scutellarin (SCU) on neurite growth and neurological functional recovery in neonatal hypoxic-ischemic (HI) rats. Primary cortical neurons were cultured to detect the effect of SCU on cell viability of neurons under oxygen-glucose deprivation (OGD). Double immunofluorescence staining of Tuj1 and TUNEL then observed the neurite growth and cell apoptosis in vitro,and double immunofluorescence staining of NEUN and TUNEL was performed to examine the neuronal apoptosis and cell apoptosis in brain tissues after HI in vivo. Pharmacological efficacy of SCU was also evaluated in HI rats by neurobehavioral tests, triphenyl tetrazolium chloride staining, Hematoxylin and eosin staining and Nissl staining. Astrocytes and microglia expression in damaged brain tissues were detected by immunostaining of GFAP and Iba1. A quantitative real-time polymerase chain reaction and western blot were applied to investigate the genetic expression changes and the protein levels of autophagy-related proteins in the injured cortex and hippocampus after HI. We found that SCU administration preserved cell viability, promoted neurite outgrowth and suppressed apoptosis of neurons subjected to OGD both in vitroand in vivo. Meanwhile, 20 mg/kg SCU treatment improved neurological functions and decreased the expression of astrocytes and microglia in the cortex and hippocampus of HI rats. Additionally, SCU treatment depressed the elevated levels of autophagy-related proteins and the p75 neurotrophin receptor (p75NTR) in both cortex and hippocampus. This study demonstrated the potential therapeutic efficacy of SCU by enhancing neurogenesis and restoring long-term neurological dysfunctions, which might be associated with p75NTR depletion in HI rats.


Assuntos
Animais Recém-Nascidos , Apigenina/farmacologia , Apigenina/uso terapêutico , Encéfalo/fisiopatologia , Glucuronatos/farmacologia , Glucuronatos/uso terapêutico , Hipóxia-Isquemia Encefálica/tratamento farmacológico , Hipóxia-Isquemia Encefálica/genética , Neurogênese/efeitos dos fármacos , Crescimento Neuronal/efeitos dos fármacos , Neurônios/efeitos dos fármacos , Animais , Apoptose/efeitos dos fármacos , Autofagia/efeitos dos fármacos , Autofagia/genética , Encéfalo/citologia , Encéfalo/metabolismo , Células Cultivadas , Modelos Animais de Doenças , Hipóxia-Isquemia Encefálica/fisiopatologia , Proteínas do Tecido Nervoso/metabolismo , Neurônios/fisiologia , Ratos , Receptores de Fatores de Crescimento/metabolismo
4.
Zhongguo Zhong Yao Za Zhi ; 39(18): 3626-9, 2014 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-25532408

RESUMO

There are few articles or reports collecting evidence about Kudiezi injection from premarketing and postmarketing research or studies systematically. This article is an exact miniature of a systematical report about Kudiezi injection. We analyzed information from four aspects, such as quality control reports, non-clinical premarketing safety experiments, postmarketing research (efficacy studies, hospital information system data and national spontaneous reporting system data), and literature analysis. All the four aspects build an evidence body for Kudiezi injection in order to inform its safety use in clinical practice and further study.


Assuntos
Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/efeitos adversos , Sistemas de Informação Hospitalar , Humanos , Injeções
5.
Brain Res ; 1552: 41-54, 2014 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-24457043

RESUMO

ß-Asarone is an active component of the Acori graminei rhizome that is a traditional Chinese medicine clinically used in treating dementia in China. However, the cognitive effect of ß-asarone and its mechanism has remained elusive. Here, we used asenescence-accelerated prone 8 (SAMP8) mice, which mimic many of the salient features of Alzheimer׳s disease (AD), to further investigate whether modulation of the ROCK signaling pathway and/or autophagy, synaptic loss is involved in the effects of ß-asarone on learning and memory. SAMP8 mice at the age of 6 months were intragastrically administered by ß-asarone or a vehicle daily for 2 months. Senescence-accelerated-resistant (SAMR1) mice were used as the control. Our results demonstrate that autophagy and ROCK expression were increased significantly in 8 months SAMP8 mice, which were concomitant with that SAMP8 mice at the same age displayed a significant synaptic loss and cognitive deficits. The up-regulation of ROCK expression and autophage in the hippocampus of SAMP8 were significantly reduced by ß-asarone, and prevents synaptic loss and improved cognitive function of the SAMP8 mice. ß-asarone decreased neuronophagia and lipofuscin in the hippocampus of SAMP8 mice, but did not reduce Aß42 levels and malondialdehyde levels and superoxide dismutase activities. Moreover, suppression of ROCK2 by siRNA significantly reduced the effects of ß-asarone on the autophage and synaptic proteins expression in PC12 cells damage induced by Aß1-40. Taken together, ß-asarone prevents autophagy and synaptic loss by reducing ROCK expression in SAMP8 mice.


Assuntos
Senilidade Prematura/tratamento farmacológico , Anisóis/uso terapêutico , Autofagia/efeitos dos fármacos , Região CA3 Hipocampal/efeitos dos fármacos , Medicamentos de Ervas Chinesas/farmacologia , Proteínas do Tecido Nervoso/biossíntese , Fármacos Neuroprotetores/uso terapêutico , Sinapses/efeitos dos fármacos , Quinases Associadas a rho/biossíntese , Senilidade Prematura/enzimologia , Senilidade Prematura/psicologia , Derivados de Alilbenzenos , Peptídeos beta-Amiloides/análise , Animais , Anisóis/farmacologia , Região CA3 Hipocampal/química , Transtornos Cognitivos/etiologia , Transtornos Cognitivos/prevenção & controle , Avaliação Pré-Clínica de Medicamentos , Indução Enzimática/efeitos dos fármacos , Lipofuscina/análise , Potenciação de Longa Duração/efeitos dos fármacos , Malondialdeído/análise , Aprendizagem em Labirinto/efeitos dos fármacos , Camundongos , Proteínas Associadas aos Microtúbulos/análise , Proteínas do Tecido Nervoso/genética , Proteínas do Tecido Nervoso/fisiologia , Fármacos Neuroprotetores/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Células PC12 , Fragmentos de Peptídeos/análise , Interferência de RNA , RNA Interferente Pequeno/farmacologia , Ratos , Superóxido Dismutase/análise , Sinapses/enzimologia , Regulação para Cima/efeitos dos fármacos , Quinases Associadas a rho/antagonistas & inibidores , Quinases Associadas a rho/genética , Quinases Associadas a rho/fisiologia
6.
J Pharm Anal ; 3(4): 292-297, 2013 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29403830

RESUMO

Euphorbia ebracteolata Hayata (E. ebracteolata) is a Chinese herbal medicine used for the treatment of tumor diseases. An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) based chemical profiling approach was established for the rapid separation and characterization on phloroglucinol derivatives and diterpenes in E. ebracteolata. Three phloroglucinol derivatives and nine diterpenes were identified by exact mass measurement and were further confirmed by Ms2 data. In addition, the chemical profiles of six compounds were acquired by reference standards. Furthermore, the fragmentation rules of phloroglucinol derivatives and diterpenes of E. ebracteolata were analyzed, and each chromatographic peak was classified.

7.
Nat Prod Commun ; 7(8): 1069-74, 2012 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-22978231

RESUMO

Identifying small molecules that are neuroprotective against stroke injury will be highly beneficial for treatment therapies. A cell viability assay and gas chromatography-mass spectrometry were used to identify active small molecules in XingNaoJing, which is a well known Chinese medicine prescribed for the effective treatment of stroke. Studies have found that muscone is the active compound that prevents PC12 cell and cortical neuron damage following various injuries. Analysis of apoptosis indicated that muscone inhibited glutamate-induced apoptotic cell death of PC12 cells and cortical neurons. Fas and caspase-8 expression were upregulated following glutamate treatment in cortical neurons, and was markedly attenuated in the presence of muscone. Furthermore, muscone significantly reduced cerebral infarct volume, neurological dysfunction and inhibited cortical neuron apoptosis in middle cerebral artery occluded (MCAO) rats in a dose-dependent manner. Moreover, a significant decrease in Fas and caspase-8 expression in the rat cortex was observed in MCAO rats treated with muscone. Our results demonstrate that muscone may be a small active molecule with neuroprotective properties, and that inhibition of apoptosis and Fas is an important mechanism of neuroprotection by muscone. These findings suggest a potential therapeutic role for muscone in the treatment of stroke.


Assuntos
Cicloparafinas/farmacologia , Fármacos Neuroprotetores/farmacologia , Acidente Vascular Cerebral/tratamento farmacológico , Receptor fas/antagonistas & inibidores , Animais , Encéfalo/efeitos dos fármacos , Encéfalo/patologia , Lesões Encefálicas/tratamento farmacológico , Masculino , Células PC12 , Ratos , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase em Tempo Real , Traumatismo por Reperfusão , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Acidente Vascular Cerebral/patologia
8.
Biol Pharm Bull ; 35(8): 1328-35, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22863933

RESUMO

Molecules that enhance chondrogenic differentiation in mesenchymal stem cells (MSCs) were identified and isolated using an in vitro Gli reporter gene assay in MSCs incorporating a Sonic Hedgehog (Shh) target. Atractylenolide III, which promoted Gli1-mediated transcriptional activity, was isolated from an ethyl acetate extract of the Rhizoma, Atractylodis macrocephalae. After dehydration, atractylenolide III was transformed to atractylenolide I. Both atractylenolides were confirmed by MS, UV, IR, 1H- and 13C-NMR spectra. Atractylenolide III (which contains -OH at the 8-position) and atractylenolide I (which lacks -OH at the 8-position) were found to effectively promote the activity of the Gli promoter. While the hydroxyl group of atractylenolide III was not essential for the effect of atractylenolide, its effect was dependent on Shh signaling. Phenotypic cellular analysis indicated that atractylenolides induced MSCs to differentiate into chondrocytes, as shown by increased expression of specific chondrogenic markers including collagen II, aggrecan and the cartilage related transcription factor, Sox9. Atractylenolides significantly increased the expression of Shh and its target gene Gli-1, indicating that Shh signaling was activated by atractylenolides. Moreover, inhibition of Shh signaling reduced the effect of atractylenolides on the chondrogenic phenotype. The discovery that atractylenolides induce chondrocytes from MSCs is promising for bony disease therapy.


Assuntos
Atractylodes/química , Diferenciação Celular/efeitos dos fármacos , Condrócitos/efeitos dos fármacos , Proteínas Hedgehog/metabolismo , Lactonas/farmacologia , Células-Tronco Mesenquimais/efeitos dos fármacos , Extratos Vegetais/farmacologia , Sesquiterpenos/farmacologia , Animais , Biomarcadores/metabolismo , Diferenciação Celular/genética , Condrócitos/citologia , Proteínas Hedgehog/genética , Fatores de Transcrição Kruppel-Like/genética , Fatores de Transcrição Kruppel-Like/metabolismo , Lactonas/isolamento & purificação , Células-Tronco Mesenquimais/citologia , Regiões Promotoras Genéticas , Ratos , Ratos Sprague-Dawley , Rizoma , Sesquiterpenos/isolamento & purificação , Transdução de Sinais/efeitos dos fármacos , Transcrição Gênica/efeitos dos fármacos , Proteína GLI1 em Dedos de Zinco
9.
Zhongguo Zhen Jiu ; 30(7): 595-7, 2010 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-20862946

RESUMO

Guinea is the first country who established diplomatic relations with P. R. China in sub-Saharan Africa. China and Guinea have maintained good relationship for the past years. Chinese Ministry of Health has sent twenty-one medical teams to Guinea since 1968. This article describes the development of acupuncture in Guinea. Based on the status of acupuncture in the Hospital Ignace Deen, in which the author had worked, the general situation, the current problems, and the development trends of acupuncture treatment in Guinea are introduced. The problems and future development trends of acupuncture in Guinea are discussed.


Assuntos
Terapia por Acupuntura , Acupuntura , Acupuntura/educação , Terapia por Acupuntura/instrumentação , Terapia por Acupuntura/normas , Guiné , Humanos
10.
Zhong Yao Cai ; 29(8): 844-6, 2006 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-17076247

RESUMO

OBJECTIVE: To establish the fingerprint of soup of Danggui Buxue decoction. METHODS: HPLC with Nucleodur C18 Gravity colum was used and the Acetonitrile-water (gradient elution) as a mobile phase and detecting wavelength at 203 nm. RESULTS: There were 15 main peaks in the soup of Danggui Buxue decoction. 15 come from Radix astragali and 7 come from Radix angelicae sinensis. CONCLUSION: This fingerprint can be used as a reference for stablility of soup of Danggui Buxue decoction.


Assuntos
Angelica sinensis/química , Medicamentos de Ervas Chinesas/química , Plantas Medicinais/química , Saponinas/análise , Astragalus propinquus/química , Cromatografia Líquida de Alta Pressão/métodos , Estabilidade de Medicamentos , Medicamentos de Ervas Chinesas/isolamento & purificação , Controle de Qualidade , Reprodutibilidade dos Testes , Saponinas/isolamento & purificação
11.
Zhongguo Zhong Yao Za Zhi ; 29(8): 764-8, 2004 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-15506289

RESUMO

OBJECTIVE: To establish the method of fingerprint analysis on volatile oil in rhizome of Acorus tatarinowii by GC-MS, and to study the main characteristic components. METHOD: The main components of 10 samples were determined by GC-MS. RESULT: The injector temperature was 250 degrees C. The interface temperature was 230 degrees C. The column flow was 1.3 mL x min(-1). The column pressure was 80 kPa. The detector volt was 1.4 kV. The temperature rate was 3 degrees C x min(-1). And the main characteristic components were composed of the methyleugenol (2.13%), cis-methylisoeugenol (4.48%), trans-methylisoeugenol (0.82%), gamma-asarone (4.51%), beta-asarone (66.15%), alpha-asarone (6.35%). And the RSD of precision and reproducibility and stability was almost in the range of 5%. CONCLUSION: The method is reliable, accurate and can be used for fingerprint analysis of volatile oil of Acorus tatarinowii.


Assuntos
Acorus/química , Eugenol/análogos & derivados , Óleos Voláteis/química , Plantas Medicinais/química , Derivados de Alilbenzenos , Anisóis/análise , Eugenol/análise , Cromatografia Gasosa-Espectrometria de Massas , Óleos Voláteis/isolamento & purificação , Rizoma/química
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