Expression of chicken epidermal growth factor (cEGF) in Escherichia coli regulates the microflora structure of the duodenum to improve growth performance and intestinal morphogenesis in broilers.

1. A study used gene synthesis to obtain the functional domains of chicken epidermal growth factor (cEGF) and examined their impact on broiler growth performance, small intestinal morphology, digestive enzyme activities in the intestinal contents and the structure of duodenal microflora.2. The pET-32a-cEGF recombinant expression vector was constructed. The specific band at 26 KDa was shown by SDS-PAGE analysis and WB results. The purified protein content was shown to be 1687 µg/ml by assay.3. A total of 180 healthy, one-day-old Arbor Acres male, white-feathered broilers were randomly divided into three dietary treatment groups (six replicate pens, 10 birds per replicate): A control diet (ND); cEGF diet (cEGF), control supplemented with 250 mg/kg cEGF and the control diet (CD) supplemented with 250 mg/kg chlortetracycline.4. The results showed that feeding the cEGF and CD diet reduced FCR of broilers aged 1-21 d, average daily feed intake (ADFI) at 22-42 d, and the FCR in the whole period (1-42 d; p < 0.05). Compared with the ND group, the cEGF diet increased duodenal α-amylase and alkaline phosphatase activities in the 1-21 d, duodenal lipase, alkaline phosphatase, and ileal alkaline phosphatase activities in the post-period and increased villus height in the duodenum and ileum (p < 0.05). In addition, the ACE and Chao1 index for the birds fed cEGF were higher than the ND group (p < 0.05). At the phyla level, Firmicutes and Proteobacteria were dominant in all groups. At the genus level, the dominant genus was Lactobacillus. The LEfSe analysis showed that the cEGF group was enriched by 11 species including Brevibacillus, Eisenbergiella, Cloacibacterium, Butyricoccus spp.5. The addition of 250 mg/kg cEGF to the diet can increase growth performance by improving intestinal development and digestive enzyme activity, which may be related to the duodenal intestinal microflora. Therefore, cEGF is an effective alternative to antibiotics in broiler farming.

Chemical and mineralogical composition of the Mongolian rural soils and their uranium sorption behavior.

Distribution of uranium (VI) between soil solids and solutions is a key parameter in assessing the risk to the biosphere of disposing uranium-rich waste products from nuclear plants as well as uranium (U) ore mining. Both of these topics have recently been brought to public attention in Mongolia. Regional background levels of soil elements are an important dataset for accessing the actual environmental situation and monitoring pollution levels. Little information, however, is available on background concentrations of various elements in Mongolian soils. Thirteen rural soils were sampled from six provinces in Mongolia, and the concentrations of macro-, micro- and trace elements were measured. The values obtained served as a reference (baseline) for uncontaminated soils. The soils were characterized with slightly acidic to strongly alkaline pH values. With the exception of the sample from a western province, all the soils investigated contained little organic matter. The content of soil elements did not vary widely among geographical regions. The concentration of most micro elements was within the range of worldwide soil values but the value for Zn tended to be moderately higher. The U (VI) sorption into the soils was investigated using the batch technique and the (237)U radionuclide tracer, produced by the photo fission reaction (238)U(γ, n) (237)U at an electron accelerator. The (237)U distribution coefficient (K(d)), derived from the sorption isotherms, was related to solution pH and varying from 9 to 2547 mL g(-1) when the pH ranged between 3 and 7.7. The sorption process was interpreted in terms of the formation of different U (VI) species at given concentrations, calculated using the Speciation program with and without carbonate in the system. The U sorption isotherm displayed two general patterns: one where sorption decreased as solution pH increased, showing a maximum at pH 3, and another pattern revealed an adsorption maximum at pH 5 and then decreased up to pH 7.7 (the final solution pH). The observed decrease in K(d) when solution pH increased from 6 to 8 was consistent with the increased formation of soluble UO(2)(OH)(2) species. A linear negative correlation between lgK(d) and the solution pH was observed similarly to that reported for the soils with a pH ≥ 6.

[Clinical study of TCM-WM on aplastic anemia complicated with hepatitis C].

The testing kit of second generation for serum anti-HCV was used in 82 cases of aplastic anemia (AA). The results showed that positive rate was 69.4% (43/62) in the patients of AA with transfusion, this was significantly higher than that in the patients of AA without transfusion. There was no difference of anti-HCV antibody positive rate between chronic AA and acute AA (P > 0.05), incidence rate of post-transfusion hepatitis C (PTHC) in AA was 33.9% (21/62), among which the incidence rate in acute and chronic AA were 68.8% (11/16) and 21.7% (10/46) respectively (P < 0.01). The anti-HCV positive patients were divided into two groups: PTHC and non-PTHC, there was no statistical difference of their transfusion volume, hemoglobin, white blood cell between these groups. Response rate of AA was lower in anti-HCV positive patients than that in negative patients (P < 0.05). Acute, icteric PTHC was predominant in patients with AA. The patients with AA complicated with PTHC was liable to bleed and be infected. PTHC has been an important complication in patients with AA. The better response was obtained by TCM-WM therapy in the patients.