[The effect of melatonin on enhancing immune function and inhibiting the ability of NO over-release in morphine dependent mice].

AIM: To observe the effects and mechanism of melatonin (MT) on the immune function of morphine dependent mice. METHODS: A physical dependent mice model was established by repeated subcutaneous injection of morphine. The intensity of morphine withdrawal syndrome was evaluated according to the weight of immune organs, the proliferation reaction of stimulated splenic lymphocytes by Con A, the phagoindex of blood primed macrophages and the content of NO induced in the peritoneal macrophage (pM phi). RESULTS: MT reversed the inhibitory effect of morphine on the proliferation ability of splenic lymphocytes and enhanced the phagocytosis of macrophages of morphine dependent mice obviously and prevented the over-release of NO from pM phi. The enhancing effects of MT on the phagocytosis can be prevented by naloxon. CONCLUSION: MT can significantly enhance the immune function of morphine dependent mice and inhibit NO excessive release from pM phi.

[Effects of jiang tang tong mai ning on serum ascorbyl radical, superoxide dismutase activity, lipid peroxide level and tissue free oxygen radical in diabetic rats].

The effect of Jiang Tang Tong Mai Ning treatment on body weight, the level of blood sugar, the serum ascorbyl radical, SOD activity, LPO level and peroxidic radial (O2.-) of heart and kidney tissue in alloxan induced diabetic rats were observed. The results were also compared with that of Jiang Tang Jia Pian and vitamin E treated groups, which showed that the level of blood sugar, the level of serum ascorbyl radical, the concentration of LPO and peroxidic radical (O2.-) of myocardium and kidney tissue decreased significantly respectively (P < 0.01, 0.01, 0.01, 0.05), and the body weight, SOD activity increased obviously after the treatment (P < 0.05). For the first time, a W-wave between S-peak and T-peak of ESR spectrum in the tissue of rat, which is similar to the form of Fe-Mo-flavoprotein has been observed in this investigation, a positive correlation appears to exist between A value and the corresponding G value of W-wave.