RESUMO
OBJECTIVE: To study the distribution law of epigoitrin in roots of Isatis indigotica of different breed types and provide a scientific basis for screening of high-quality Isatis indigotica breed. METHODS: Determined the contents of epigoitrin in tap root and lateral root of Chinese-cabbage-leaf Isatis, cabbage-leaf Isatis, mustard-leaf Isatis and tetraploid Isatis by HPLC. Also, compared the contents of epigoitin in xylem and phloem of Isatis indigotica. RESULTS: Contents of epigoitrin in the tap root and lateral root of Isatis indigotica of the different breed types were significant different. In four breed types of Isatis indigotica, contents of epigoitrin in the tap root and phloem were higher than those in the lateral root and xylem, respectively. CONCLUSION: Contents of epigoitrin in the lateral root of Isatis indigotica are higher than those of tap root and epigoitrin distributes mainly in phloem.