RESUMO
Potential xanthine oxidase (XOD) inhibitors in Lagotis brevituba were captured by using affinity and ultrafiltration. The structures of the captured components were identified by ultra-performance liquid chromatography coupled with Q-TOF mass spectrometry (UPLC-Q-TOF-MS). The binding intensity and binding mechanism between the captured components and XOD were analyzed by using molecular docking software Autodock 4.2. A total of 17 compounds were identified, including 9 flavonoids, 5 phenolic acids and 3 triterpenes. Molecular docking results showed that all the captured components could be spontaneously bound with XOD mainly via hydrogen bond, Van der Waals' force and hydrophobic interaction. From the perspective of binding energy and scoring function, the collected fractions all had potential prospects for XOD inhibitors, and the flavonoid luteolin-3',7 glucuronide had the best effect. The results also showed that affinity and ultrafiltration, ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) and molecular docking technology can provide a powerful tool for the analysis of XOD inhibitor components in natural products.
Assuntos
Flavonoides/análise , Compostos Fitoquímicos/análise , Plantaginaceae/química , Xantina Oxidase/antagonistas & inibidores , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Inibidores Enzimáticos/análise , Inibidores Enzimáticos/isolamento & purificação , Flavonoides/isolamento & purificação , Simulação de Acoplamento Molecular , Compostos Fitoquímicos/isolamento & purificação , Espectrometria de Massas em TandemRESUMO
Collecting different commodity grade Gardenia jasminoides of wild and cultivated varieties all over the country, obtaining color information from each batch of G. jasminoides by the standard D65 light source and image acquisition system, quantifing the gardenia plumpness information by the digital display vernier caliper, determinating 6 kinds of effective components of G. jasminoides by HPLC, classifing from ten indicators by two step clustering analysis and correspondence analysis method of statistics, clearing the importance of the traditional identification indexes, establishing multiple corresponding relation between the skin color and commercial specification of G. jasminoides,exploring the correlation of the skin color and chemical composition, to provide the reference for the reasonable division of commercial specifications and grades of G. jasminoides. Medicine is divided into two classes and has obvious distinguish meaning, The importance of the skin color is greater than the plumpness in traditional identification characteristics, it can accurately distinguish the specifications of G. jasminoides. We improve and rebuild the standard of commodity specifications and grades of Gardenia jasminoides Ellis and establish the rapid evaluation method by the study, it provide a new way and idea for the comprehensive evaluation of G. jasminoides quality.
Assuntos
Gardenia/química , Controle de Qualidade , Cromatografia Líquida de Alta Pressão , Plantas Medicinais/químicaRESUMO
The chemical constituents of Lagotis brevituba were rapidly determined and analyzed by using ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) method, providing material basis for the clinical application of L. brevituba. The separation was performed on UPLC YMC-Triart C18 (2.1 mm×100 mm, 1.9 µm) column, with acetonitrile-water containing 0.2% formic acid as mobile phase for gradient elution. The flow rate was 0.4 mLâ¢min-1 gradient elution and column temperature was 40 â, the injection volume was 2 µL. ESI ion source was used to ensure the data collected in a negative ion mode. The chemical components of L. brevituba were identified through retention time, exact relative molecular mass, cleavage fragments of MS/MS and reported data. The results showed that a total of 22 compounds were identified, including 11 flavones, 6 phenylethanoid glycosides, 1 iridoid glucosides, and 4 organic acid. The UPLC-Q-TOF-MS/MS method could fast identify the chemical components of L. brevituba, providing valuable information about L. brevituba for its clinical application.
Assuntos
Medicamentos de Ervas Chinesas/análise , Plantaginaceae/química , Cromatografia Líquida de Alta Pressão , Flavonas/análise , Glicosídeos/análise , Glucosídeos Iridoides/análise , Espectrometria de Massas em TandemRESUMO
Seven compounds(deacetylasperulasidic acid methyl ester, gardenoside, chlorogenic acid, geniposide, crocin-â , crocin-â ¡, chikusetsu saponin â £a)were determined simultaneously by multiple wavelength HPLC with diode array detector(DAD) in different parts of Gardenia jasminoides. The results showed that these components in different parts of G. jasminoides had a different distribution, and there was a large difference in content of each component. Geniposide was mainly distributed in fruits and leaves; chikusetsu saponin â £a was mainly distributed in roots and stems; crocus glycosides existed mainly in fruits; chlorogenic acid had a higher distribution in leaves and stems; gardenoside had a higher distribution in leaves and roots, while ceacetylasperulasidic acid methyl ester had a higher distribution in roots and stems. Based on the analysis of the chemical composition and content difference in different parts of G. jasminoides, the basis for the comprehensive utilization and quality evaluation of resources of G. jasminoides was provided.
Assuntos
Frutas/química , Gardenia/química , Compostos Fitoquímicos/análise , Folhas de Planta/química , Ácido Clorogênico/análise , Cromatografia Líquida de Alta Pressão , Iridoides/análiseRESUMO
The application of ArcGIS and Maxent modelto analyze the ecological suitability of Gardenia jasminoides.Taking 85 batches of Gardenia as the basis of analysis, the selection of ecological factors for the growth of Gardenia. The results showed that the average precipitation in April, the average precipitation in November and the average precipitation in August were the most important factors affecting the growth of Gardenia. The relative concentration of Gardenia suitable growth region,north to the south of Shaanxi province, south of Henan, central Anhui, south to the north of Hainan province, west to central Sichuan province, east of Zhejiang coastal area, northeast of Taiwan.
Assuntos
Gardenia/crescimento & desenvolvimento , China , Clima , Ecologia , Sistemas de Informação GeográficaRESUMO
Objective: To provide the experimental evidence for the appropriate selection of the different prepared products from Gardenia jasminoides fruits by comparing their protection effects on carbon tetrachlorideï¼ CCl4ï¼-induced acute liver injury. Methods: The activities of ALT,AST,ADA,LDH,ALP and contents of PA,TP,TBIL,DBIL,TBA in serum,the activities of SOD and the content of MDA in liver tissue were measured in acute liver injury rats by carbon tetrachloride. Also the pathological changes of liver tissues were examined under microscope. Results: The biochemical indexes of AST,ALT,TBA,ADA,LDH and MDA were significantly improved in all groups of prepared products from Gardenia jasminoides fruits,but not SOD and ALP. The lesions of liver tissue had different degrees of reduction. Conclusion: The different prepared products from Gardenia jasminoides fruits had the effects of liver protection. The nut of Gardeniae Fructus was superior to the peel in enzyme decreasing and liver protection. The crude was superior to the stir-cooked in enzyme decreasing and liver protection.
Assuntos
Doença Hepática Induzida por Substâncias e Drogas , Frutas , Gardenia , Animais , Tetracloreto de Carbono , Medicamentos de Ervas Chinesas , Fígado , Extratos Vegetais , RatosRESUMO
OBJECTIVE: To investigate the chemical components of Gardenia jasminoides. METHODS: Various column chromatography were used in the isolation and purification, and physiochemical constant determination and spectral analysis were adopted to idenitify the chemical structures. RESULTS: Ten compounds were isolated and identified as jasminoside A(1), epijasminoside A(2), 6-O-methylscandoside methyl ester (3), 6-O-methyldeacetylasperulosidic acid methyl ester (4), gardenoside (5), phenylmethol (6), 4-hydroxy-phenylmethol-O-beta-D-glucopyranosyl- (1-->6) -beta-D-glucopyranoside (7), 3,4-dihydroxy-phenylmethol-O-beta-D-glucopyranosyl-(1-6)-beta-D-glucopyranoside (8), 3-hydroxy4-methoxy-phenylmethol-O-beta-D-glucopyranosyl-(1-->6)-beta-D-glucopyranoside (9), 3-hydroxy-4-methoxyphenylmethol-O-beta-D-glucopyranoside (10). CONCLUSION: Compounds 6 -10 are isolated from this plant for the first time.
Assuntos
Medicamentos de Ervas Chinesas/química , Frutas/química , Gardenia/química , Álcoois Benzílicos/química , Álcoois Benzílicos/isolamento & purificação , Cromatografia Líquida de Alta Pressão , Medicamentos de Ervas Chinesas/isolamento & purificação , Etanol/química , Iridoides/química , Iridoides/isolamento & purificação , Estrutura MolecularRESUMO
OBJECTIVE: To investigate the chemical components of Gardenia jasminoides. METHODS: Various column chromatography were used in the isolation and purification. Physiochemical constant determination and spectral analysis were adopted to determine the chemical structures. RESULTS: Ten compounds were isolated and elucidated as syringic acid (1), syringaldehyde (2), vanillic acid (3), 3-hydroxy-vanillic acid (4), 3, 4, 5-trimethoxy-phenol (5), 4-hydroxy-3,5-dimethoxy-phenol (6), 4-methoxy-benzaldehyde (7), 7-hydroxy-5-methoxy-chromone (8), crocin-1 (9), crocin-2 (10). CONCLUSION: Compounds 2, 4 - 8 are first isolated from this plant.
Assuntos
Medicamentos de Ervas Chinesas/química , Frutas/química , Gardenia/química , Benzaldeídos/química , Benzaldeídos/isolamento & purificação , Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/isolamento & purificação , Estrutura MolecularRESUMO
OBJECTIVE: To establish a method of HPLC fingerprint and chemical pattern recognition of liposoluble chemical components for quality evaluation of Euodiae Fructus. METHODS: The HPLC chromatographic separation was performed on a C18 column (250 mm x 4.6 mm, 5 microm). The mobile phase was a mixture of acetonitrile-0.5% acetic acid in gradient elution with flow rate of 1 mL/min. The detection wavelength was set at 249 nm, and the column temperature was 30 degrees C. RESULTS: There were 9 common peaks in the HPLC fingerprint of 34 batches of Euodiae Fructus samples. The results of cluster analysis and principal component analysis showed that the liposoluble chemical components of Euodia rutaecarpa (Juss.) Benth. var. officinalis (Dode) Huang and Euodia rutaecarpa (Juss.) Benth. var. bodinieri (Dode) Huang were more similar than that of Euodia rutaecarpa (Juss.) Benth. CONCLUSION: The results provide new experimental and theoretical basis for quality control and evaluation of Euodiae Fructus.
Assuntos
Medicamentos de Ervas Chinesas/química , Evodia/química , Plantas Medicinais/química , Cromatografia Líquida de Alta Pressão , Análise por Conglomerados , Ecossistema , Evodia/classificação , Frutas/química , Análise de Componente Principal , Controle de QualidadeRESUMO
OBJECTIVE: To investigate the effect of Zhizi Baipi soup and its disassembled prescription on protecting liver and improving choleresis and explore the regularity of Zhizi Baipi soup composition. METHODS: The model of mouse liver injury induced by carbon tetraehlofide (CCl4) was used to observe the effects of Zhizi Baipi soup and its disassembled prescription by oral adminstration, the bile volume was determinied by common bile duct drainage. RESULTS: Zhizi Baipi soup and each treatment group with gardenia could significantly inhibit the increased serum ATL and AST activities, reduce liver MDA level, and significantly promote the bile flow and bilirubin in bile in normal rats. CONCLUSION: Zhizi Baipi soup has effects on protecting liver and increasing bile secretion, its monarch drug, gardenia plays an important role in the decoction, the effect of eliminating dampness and heat are mainly ascribed to the synergic effect of gardenia and phellodendron.
Assuntos
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico , Medicamentos de Ervas Chinesas/farmacologia , Vesícula Biliar/efeitos dos fármacos , Fígado/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Alanina Transaminase/sangue , Animais , Aspartato Aminotransferases/sangue , Bile/metabolismo , Bilirrubina/metabolismo , Tetracloreto de Carbono , Doença Hepática Induzida por Substâncias e Drogas/metabolismo , Doença Hepática Induzida por Substâncias e Drogas/patologia , Modelos Animais de Doenças , Combinação de Medicamentos , Medicamentos de Ervas Chinesas/administração & dosagem , Medicamentos de Ervas Chinesas/química , Vesícula Biliar/metabolismo , Gardenia/química , Fígado/metabolismo , Fígado/patologia , Masculino , Malondialdeído/metabolismo , Camundongos , Substâncias Protetoras/administração & dosagem , Ratos , Ratos Sprague-Dawley , Rutaceae/químicaRESUMO
OBJECTIVE: To clone full-length sequence of Cu/Zn-SOD gene of Evodia rutaecarpa. METHODS: The genes ORF,3,and 5,noncoding regions were achieved using the RACE technology. RESULTS: This genes full-length was 717 bp, the open reading frame was 459 bp and 152 amino acids were encoded. The accession number of GenBank was JQ285851. CONCLUSION: This Cu/Zn-SOD gene is full-length cDNA sequence of Evodia rutaecarpa and it has high homology than other plants.
Assuntos
Evodia/genética , Genes de Plantas , Superóxido Dismutase/genética , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , DNA Complementar/genética , Evodia/enzimologia , Regulação Enzimológica da Expressão Gênica , Dados de Sequência Molecular , Fases de Leitura Aberta , Filogenia , Folhas de Planta/enzimologia , Folhas de Planta/genética , Reação em Cadeia da Polimerase , Análise de Sequência de DNA , Superóxido Dismutase/metabolismoRESUMO
OBJECTIVE: To determinate geniposide and croein-1 in Fructus Gardeniae from different breeds. METHODS: RP-HPLC method was adopted. The chromatographic separation was performed on an Eclipse XDB-C18 column (250 mm x 4.6 mm, 5 microm, Agilent). The mobile phase was a mixture of acetonitrile-0.1% phosphoric acid aqueous solution in gradient elution. The wavelength of 238 nm and 440 nm was selected to determinate geniposide and crocins simultaneously. RESULT: The contents of geniposide and croein-1 in different breeds of Fructus Gardeniae are different. CONCLUSION: This method is simple and repeatable, and could be used for the quality control of Fructus Gardeniae.
Assuntos
Carotenoides/análise , Medicamentos de Ervas Chinesas/análise , Gardenia/química , Iridoides/análise , Cromatografia Líquida de Alta Pressão/métodos , Frutas/química , Gardenia/crescimento & desenvolvimento , Reprodutibilidade dos TestesRESUMO
OBJECTIVE: To develop a high performance capillary electrophoresis method for Citrus aurantium fingerprints to control its quality. METHOD: The background electrolyte (BGE) was an 80 mmol x L(-1) boric acid solution containing 15 mmol x L(-1) borate. The pH of the BGE was adjusted to 9.70 with KOH solution. The detection wavelength was 201 nm and a voltage of 16 kV was applied. The sample hydrodynamic injection was 0.4 ps with a duration time of 8 sec. C. aurantium was extracted by water and a set of capillary electrophoresis fingerprints (CEFP) containing 12 co-possessing peaks was obtained. RESULT: There were good similarities between the standard CEFP and each set of CEFP of C. aurantium collected from eleven different places, and their similarity coefficients were between 0.973 and 0.996. CONCLUSION: The CEFP has acceptable precision, reproducibility and stability and can be used for the quality control of C. aurantium.
Assuntos
Citrus/química , Eletroforese Capilar/métodos , Boratos/química , Eletrólitos/química , Concentração de Íons de Hidrogênio , TemperaturaRESUMO
OBJECTIVE: To make sure the best extraction technology of essential oil from Mosla chinensis Maxim. METHODS: By single-factor experiment, quantity of water, degree of crush, time of extraction and soakage and the part of medicinal materials were studied. RESULTS: The best extractin technology of Mosla chinensis Maxim was established.
Assuntos
Lamiaceae/química , Óleos Voláteis/isolamento & purificação , Plantas Medicinais/química , Tecnologia Farmacêutica/métodos , Flores/química , Temperatura Alta , Caules de Planta/química , Pós , Fatores de TempoRESUMO
OBJECTIVE: To investigate the triterpenoids chemical constituents in rhizome of Alisma gramineum. METHOD: Silicon gel chromatography and HPLC techniques were employed for isolation and purification of the constituents, and the structures were elucidated by various spectral analyses such as NMR, MS and IR. RESULT: 5 protostane-type tetracyclic triterpenoids were isolated and identified as 16,23-oxidoalisol B(1), 11-deoxyalisol C(2), alisol F(3), 16beta-methoxyalisol B acetate (4), 16beta-hydroxyalisol B acetate (5). CONCLUSION: All of these compounds were found from A. gramineum for the first time.