Nano Zinc Oxide Improves Performance, IGF-I mRNA Expression, Meat Quality, and Humeral Immune Response and Alleviates Oxidative Stress and NF-κB Immunohistochemistry of Broiler Chickens.

A 35-day trial was set to explore the effects of different dietary zinc sources on growth, insulin-like growth factor I (IGF-I) mRNA expression, meat quality, immune response, antioxidant activity, and immunohistochemistry of nuclear factor kappa B (NF-κ7B) of broiler chickens. Ross 308 broiler chicks (n = 156) were randomly assigned into four experimental groups. The G1 received the basal control diet without zinc supplementation; the G2, G3, and G4 were supplemented with zinc oxide, zinc lysine, and nano zinc oxide, respectively, at a level of 40 mg Zn/kg diet. The data revealed that nano zinc oxide linearly improved the overall growth performance parameters. Nano zinc oxide linearly elevated (P < 0.001) mRNA expression of IGF-I followed by G3. The pH value of breast muscle in G4 shows a linearly decreasing value (P < 0.001). Also, the linearly highest expressible release volume percentage and lightness (L*) value with the lowest redness (a*) value (P < 0.05) were recorded in G4 and G3. A numerical increase in the total antibody titer was recorded on the 35th day in the G3 and G4. A numerical elevation in the superoxide dismutase (SOD) and a numerical reduction in the serum malondialdehyde (MDA) were recorded in the G4. The section of the liver from G4 revealed significantly very low expression of NF-κB staining. It is concluded that nano zinc oxide is considered the more trending zinc source. It had no negative effects on the health status and can be used in broiler diet premix.

Role of dietary Spirulina platensis and betaine supplementation on growth, hematological, serum biochemical parameters, antioxidant status, immune responses, and disease resistance in Nile tilapia.

Spirulina platensis is, a freshwater microalga, broadly used worldwide. It not only stimulates the immune systems of aquatic organisms but also provides a protein-rich diet and commonly used in the manufacture of aquafeeds. This study was planned to evaluate the growth performance, hepato-renal, and immune response biomarkers of Spirulina and Betaine on Nile tilapia (Oreochromis niloticus) and their protective effect against infection with Aeromonas hydrophila. O. niloticus juveniles (20.22 ± 0.86 g) were divided into four groups (n = 10 per replicate). For 8 weeks, the first and second groups (TS&TB) were fed with 0.5% and 0.3% concentrations of Spirulina and Betaine supplemented diets, respectively; the third group (TSB) was fed with a Spirulina and Betaine mixed diet; the fourth group was fed with a basal diet (without supplementation, T0), which served as control. Dietary inclusion of Spirulina and Betaine significantly improved (P ˂ 0.05) the weight gain, final weight, and food conversion ratio, especially in the TS group. The activities of hepatic malonaldehyde were unchanged in TS & TSB groups and the muscular significantly decreased (P ˂ 0.05) in the same groups, while both increased in the TB group; meanwhile, levels of glutathione reductase were significantly upregulated in all treated groups. Serum interleukins, TNF- alpha, and IL-10 levels were also significantly reduced in all treatment groups. A significant protective power against pathogenic Aeromonas infection was evidenced in all treated groups. Findings in this study highlight the reputation of Spirulina and Betaine as immunostimulants and protective agents against A. hydrophila infection in O. niloticus.

Impacts of dietary supplementation with nano-iron and methionine on growth, blood chemistry, liver biomarkers, and tissue histology of heat-stressed broiler chickens.

A 28-day study was done to explore the impact of nano-iron alone or combined with methionine on growth, blood chemistry, liver biomarkers, and tissue histology of heat-stressed chicken. One-day-old Ross 308 chicks were randomly allocated to three groups. Each group was divided into three replicates (13 chicks/replicate). The first group was the control one that was fed a basal diet without supplementation (T0). The second group was fed a basal diet with nano-iron 4 mg kg-1 diet (T1). The third group was fed a basal diet with nano-iron 4 mg kg-1 diet plus methionine 4 g kg-1 diet (T2). The results showed that the birds in the control group had significantly (p < 0.05) higher final weights. Also, a partial relief of heat stress adverse effects was observed on growth by T1 compared to T2. The T2 showed a significantly increased (p < 0.05) free iron (Fe) level and transferrin saturation index. Likewise, T2 significantly (p < 0.05) reduced total iron-binding capacity (TIBC) and transferrin level in comparison with T0 and T1. Also, hepatic impairment and inflammatory response were observed in the T2 group when compared to T0 and T1, besides a bad lipid profile. Further, T2 showed raised levels of Fe and ferritin in their hepatic tissues compared to those T1 and T0. A significant increment of thiobarbituric acid reactive and decrement of reduced glutathione levels in the hepatic tissues of T2 and T1 versus T0 levels were recorded. It is concluded that nano-iron at the level of 4 mg kg-1 in this study is highly absorbed, leading to harmful effects. Further investigations are needed to detect the proper supplemental level.

Nanoselenium effect on growth performance, carcass traits, antioxidant activity, and immune status of broilers.

A 42-day comparative study was conducted to assess the impact of nanoselenium to other selenium sources on performance, antioxidant activity, immunity, and carcass traits in broilers. Ross 308 (n = 156) 1-day-old broiler chicks, with average initial body weight of 45.80 ± 0.35, were randomly allocated to 4 groups. The first group (G1) fed the basal diet without selenium supplementation. The second group (G2), the third group (G3), and the fourth group (G4) were supplemented with dietary selenium at the level of 0.3 mg kg-1 diet in the form of sodium selenite, seleno-methionine, and nanoselenium, respectively. The results revealed significant improvement on most of the performance parameters of nanoselenium at the level of 0.3 mg kg-1 diet (P < 0.05). Nanoselenium and seleno-methionine achieved the best dressing %, spleen index %, and thymus index %. Concerning to internal organ indices, none of these indices was significantly affected by any selenium sources (P < 0.05). Glutathione peroxidase (GSH-PX) activity and malondialdehyde (MDA) content were not significantly affected by different selenium sources among all experimental groups. Serum interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) showed significant (P < 0.05) decrease in nanoselenium supplemented group compared with other groups. In case of serum IL-10 level, a significant (P < 0.05) increase was reported in nanoselenium supplemented group followed by G3 then G2. There were no statistical differences in the serum alanine transaminase, aspartate transaminase, total protein, albumin concentration, serum creatinine level, and uric acid concentration levels among all experimental groups. It is concluded that nano selenium can be a useful and better source of selenium for broilers.

Spirulina (Arthrospira platensis) supplementation improves growth performance, feed utilization, immune response, and relieves oxidative stress in Nile tilapia (Oreochromis niloticus) challenged with Pseudomonas fluorescens.

One hundred and eighty Nile tilapia fish were used in eighty-three-day growth trial. Fish were divided into three treatment groups. The first group T0 was given the basal diet without any supplementation and served as the control group. The second group T1 was given the basal diet supplemented by 1% Spirulina. The third group T2 was given the basal diet supplemented by 2% Spirulina. At the end of the growth performance trial, a challenge trial was conducted using virulent strain of Pseudomonas fluorescens. Clinical signs, mortalities, postmortem lesions and histopathological alterations were recorded. Hematological, biochemical, oxidative stress and immunological parameters were measured after challenge with Pseudomonas fluorescens. Growth performance was non significantly improved in tilapia fed the diet with 1% Spirulina supplementation (T1). There were neither signs nor mortalities among fishes belonging to 1% Spirulina challenged group. The results showed that Spirulina has a positive effect on hematological, biochemical parameters, MDA, SOD and CAT at T1 (1% spirulia) rather than T2 (2%spirulia). Moreover, the results indicate that Spirulina 1% enhanced bactericidal, phagocytic and lysozyme activities conferring protection against infection. Our results demonstrated a significant up-regulation of pro-inflammatory cytokine (IL-1ß and TNF-α) and a down-regulation of anti-inflammatory cytokine (IL-10). We concluded that 1% Spirulina supplementation significantly improved immunity of Nile tilapia against Pseudomonas fluorescence than 2% Spirulina supplementation.