Dissipation and risk assessment of fluopyram and trifloxystrobin on onion by GC-MS/MS.

The dissipation and risk assessment studies on fluopyram, trifloxystrobin and their metabolites were carried out on onion under field conditions after two treatments of fluopyram 250 g/L + trifloxystrobin 250 g/L SC @ 150 and 300 g a.i. ha-1. The onion bulb samples were collected at 0, 3, 7, 14, and 21 days after second spray to study the pattern of dissipation using QuEChERS methodology for processing and analysis on GC-MS/MS. The total initial residues of fluopyram (fluopyram + fluopyram benzamide) in immature onion bulb were 2.14 and 4.93 mg kg-1, at single and double dose, respectively. The residues of 0.02 and 0.06 mg kg-1 persisted in the mature onion bulb collected at the harvest (30 days after treatment). The total initial residues of trifloxystrobin (trifloxystrobin + CGA 321113) in immature onion bulb were 0.65 and 1.97 mg kg-1, at single and double dose, respectively, which reached < LOQ and 0.06 mg kg-1 at the respective doses at the harvest time. Dissipation of fluopyram followed second-order kinetics with DT50 values of 1.83 and 1.74 days, whereas trifloxystrobin followed first-order kinetics with DT50 values of 4.73 and 4.78 days, at single and double dose respectively. Risk assessment in terms of hazard quotient was done to estimate the risk that can occur due to application of this combination pesticide. It was observed that even the spray at the double recommended dose could not have dietary risks on the consumers.

Effect of dehydrogenase, phosphatase and urease activity in cotton soil after applying thiamethoxam as seed treatment.

Soil enzymes are indicators of microbial activities in soil and are often considered as an indicator of soil health and fertility. They are very sensitive to the agricultural practices, pH of the soil, nutrients, inhibitors and weather conditions. To understand the effect of an insecticide, thiamethoxam, on different soil enzyme activities, the experiments were conducted at cotton experimental fields of Punjab Agricultural University, Ludhiana. The results here were presented to understand the impact of thiamethoxam on soil enzyme activities. Thiamethoxam was applied as seed treatment to control the pest. Soil from three localities, i.e. soil in which seed was treated with recommended dose at 2.1 g a.i. kg(-1), soil in which seed was treated with four times recommended dose at 8.4 g a.i. kg(-1) and from the control field, were tested for different enzyme activities. Phosphatase and dehydrogenase activities were high in control soil in comparison to control soil while no effect of this insecticide on urease activity. Thiamethoxam had inhibitory effects on dehydrogenase and phosphatase activities. Therefore, it can be attributed that agricultural practices, weather conditions and use of thiamethoxam might be responsible for the different level of enzyme activities in soil.

Residual behavior of quizalofop ethyl on onion (Allium cepa L.).

Quizalofop ethyl, a phenoxy propionate herbicide, is used for postemergence control of annual and perennial grass weeds in broad-leaved crops in India. The experiments were designed to study the dissipation kinetics of quizalofop ethyl on onion for two seasons. A simple, rapid, and sensitive method for estimation of quizalofop ethyl residues in onion and soil was developed and validated. The recoveries of quizalofop ethyl residues from onion and soil at different spiking level range from 84.81 to 92.68 %. The limit of quantification of this method was found to be 0.01 µg g(-1). The risk assessment through consumption of the onion in comparison to its acceptable daily intake which is an important parameter for the safety of the consumer was also evaluated. Standardized methodology supported by recovery studies was adopted to estimate residues of quizalofop ethyl on onion and soil. The average initial deposits of quizalofop ethyl on onion were observed to be 0.25 and 0.33 mg kg(-1), following single application of the herbicide at 50 g active ingredient (a.i.) ha(-1) during 2009 and 2010, respectively. The half-life values (T (1/2)) of quizalofop ethyl on onion crop were worked out to be 0.85 and 0.79 days, respectively, during 2009 and 2010. At harvest time, the residues of quizalofop ethyl on onion and soil were found to be below the determination limit of 0.01 mg kg(-1) following single application of the herbicide at 50 and 100 g a.i. ha(-1) for both the periods.

Direct estimation of carbaryl by gas liquid chromatography with nitrogen phosphorus detection.

A simple and efficient analytical method was standardized for the estimation of residues of carbaryl in various substrates comprising grape berries, kinnow pulps, kinnow rind and soil. The samples were refluxed using mixture of methanol: 0.5 N HCl (1:1 v/v); diluted with brine solution, partitioned into chloroform and dried over anhydrous sodium sulfate. Further the samples were treated with anhydrous magnesium sulfate and primary secondary amine. Final clear extracts were concentrated under vacuum and reconstituted the volume into acetone. The residues were estimated directly on gas liquid chromatograph equipped with nitrogen phosphorus detection system equipped with a capillary column packed with 5 % diphenyl 95 % dimethyl polysiloxane non-polar phase. A consistent recovery from 82 % to 97 % for carbaryl was observed when samples were spiked at levels ranging from 0.05 to 1.00 mg kg(-1). The limit of quantification of the method was worked out to be 0.05 mg kg(-1) for grape berries, kinnow pulp, kinnow rind and soil.