Sexual experience in female mice involves synaptophysin-related plasticity in the accessory olfactory bulb.

Sexually naïve female mice do not display high levels of sexual receptivity in their first sexual experience; they require around 4-5 sexual encounters to display the full receptive response, assessed by the lordosis reflex. In this study, we evaluated if repeated sexual stimulation with the same male is associated with changes in synaptic remodeling evaluated by synaptophysin (SYP) in brain structures involved in the control of sexual behavior such as the main and accessory olfactory bulbs (MOB and AOB, respectively), medial preoptic area (MPOA), ventromedial hypothalamus (VMH), and amygdala (AMG). Female mice were ovariectomized and hormonally primed to induce sexual receptivity. They were randomly distributed into three groups: a) sexually naïve (SN), with no prior sexual stimulation; b) sexually inexperienced (SI), with one prior mating session; and c) sexually experienced (SE), with six mating sessions. The SI group showed a significant decrease in SYP in the glomerular, mitral and granular layers of the AOB in comparison to SN and SE females. SYP expression increased in the SE group in comparison to SN and SI females in the glomerular and mitral cell layers of the AOB. No significant differences between groups were found in the other brain regions (MOB, MPOA, VMH or AMG). These changes in SYP expression in the AOB suggest that plastic modifications in this brain region can be associated with receptivity increase in sexual experience in female mice.

Sexual experience with a known male modulates c-Fos expression in response to mating and male pheromone exposure in female mice.

Sexually naïve female mice are not sexually receptive in their first mating opportunity. Four to five sexual encounters are needed to display high sexual receptivity as assessed by the lordosis reflex. The neuronal changes induced by sexual experience are not well understood. In this study, we evaluated if repeated sexual stimulation with the same male was associated with an increase in the neuronal activity evaluated by c-Fos expression in brain structures associated with the control of sexual behavior such as the accessory olfactory bulb (AOB), ventromedial hypothalamus (VMH), and the medial preoptic area (MPOA). Ovariectomized female mice were randomly distributed into three groups: sexually naïve (SN), with no prior sexual stimulation; sexually inexperienced (SI), with one prior mating session; and sexually experienced (SE), with six prior mating sessions. Females were primed with estradiol benzoate and progesterone once a week for 7 weeks. Neuronal activation in response to mating or soiled bedding was evaluated in the 7th week. Each group was subdivided into three subgroups: clean (exposure to clean bedding), male bedding (exposure to sawdust soiled with secretions from a male), or mating. Each female mated with her assigned male; in the exposure subgroup, soiled bedding was obtained from the male with whom she mated. Neuronal activity data showed that SE females had a higher c-Fos response in the VMH when they mated in comparison to females exposed to clean bedding. SI females that mated had a decrease c-Fos expression in the glomerular cell layer of the AOB, compared to females exposed to male bedding. The mitral cell layer showed a higher c-Fos response in SI females that mated in comparison to those exposed to male bedding. Comparisons between groups presented with the same stimulus indicate that SI females exposed to male bedding showed a decrease in c-Fos response in the mitral cell layer in comparison to SE and SN females. Correlation analysis demonstrated that the lordosis quotient from the last mating test correlated positively with the number of c-Fos-positive cells in the mitral cell layer in SE and SI groups. A similar correlation was found in the MPOA in SI females. Prior mating in female mice is required to increase sexual receptivity. Changes in the neuronal activity in the AOB and VMH may be involved in the neuronal plasticity induced by repeated sexual stimulation.