RESUMO
AIM: Study the composition of plant extracts using high-performance liquid chromatography. (HPLC) and evaluation of their antimicrobial effect against Vibrio cholerae El Tor. MATERIALS AND METHODS: Qualitative and quantitative composition of plant extracts was studied using HPLC. Determination of sensitivity of microorganisms to plant extracts was carried out by diffusion into agar method and serial dilutions method. RESULTS: Antibacterial effect of water, water-alcohol and acetone extracts of roots of Limonium gmelinii L., Berberis vulgaris L. and Glycyrrhiza glabra L. was studied. The most effective methods of extraction of biologically active substances, possessing antimicrobial effect against various strains of V. cholerae El Tor, were determined. CONCLUSION: The use of HPLC allowed to establish the presence of catechines, alkaloids protoberberines and glycyrrhizic acid in xtracts, possessing antimicrobial effect against V. cholera El Tor strains.
Assuntos
Antibacterianos/química , Alcaloides de Berberina/química , Catecóis/química , Ácido Glicirrízico/química , Extratos Vegetais/química , Vibrio cholerae/efeitos dos fármacos , Acetona/química , Antibacterianos/isolamento & purificação , Antibacterianos/farmacologia , Alcaloides de Berberina/isolamento & purificação , Alcaloides de Berberina/farmacologia , Berberis/química , Catecóis/isolamento & purificação , Catecóis/farmacologia , Cromatografia Líquida de Alta Pressão , Etanol/química , Glycyrrhiza/química , Ácido Glicirrízico/isolamento & purificação , Ácido Glicirrízico/farmacologia , Testes de Sensibilidade Microbiana , Plumbaginaceae/química , Solventes/química , Vibrio cholerae/crescimento & desenvolvimento , Água/químicaRESUMO
AIM: Study of plant extracts that have the ability to neutralize cytotoxic activity of hemolysin. MATERIALS AND METHODS: Preparations of purified and recombinant V. cholerae eltor hemolysin as well as supernatants of V. cholerae strains were used. Determination ofcytotoxic activity of hemolysin and neutralizing activity of plant extracts were carried out by using cell cultures CHO-K1 and CaCo2. RESULTS: Out of 9 water extracts only 3 - extracts of Rhei rhizome, Limonium gmelinii and Quercus robur neutralized hemolysin in cell culture CHO-K1 and CaCo2, whereas the other extracts--Humulus lupulus, Ocimum basilicum, Chelidonium majus, Juglans regia, Achillea milefolium and Hypericum perforatum did not have anti-cytotoxic effect. Neutralizing properties of extracts are exhibited during their co-incubation with hemolysin preparations and supernatants of V. cholerae strains already within 10 minutes. CONCLUSION: Plant extracts that have anti-cytotoxic activity against hemolysin are perspective for development oftherapeutic-prophylaxis preparations.
Assuntos
Proteínas de Bactérias/efeitos dos fármacos , Cólera/tratamento farmacológico , Proteínas Hemolisinas/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Proteínas de Bactérias/toxicidade , Células CHO , Células CACO-2 , Cólera/microbiologia , Toxina da Cólera/antagonistas & inibidores , Toxina da Cólera/química , Cricetinae , Cricetulus , Proteínas Hemolisinas/toxicidade , Humanos , Extratos Vegetais/química , Plumbaginaceae/química , Quercus/química , Vibrio cholerae/efeitos dos fármacos , Vibrio cholerae/patogenicidadeRESUMO
AIM: Study the activity of plant extracts against cholera toxin (CT) of Vibrio cholerae O1. MATERIALS AND METHODS: Antitoxic activity of plant extracts was determined by using enzyme immunoassay and CHO-K1 cell culture. RESULTS: 8 water extracts of plants were studied. Extracts of nut, tutsan, milfoil, basil do not have effect on CT activity in EIA or CHO-K1 cell culture. Celandine and rhubarb extracts do not reduce CT immunochemical activity but prevent elongation of CHO-K1 cells. Oak and hop extracts suppress binding in EIA of cholera toxin and GM1 receptors and insignificantly reduce its activity in cell culture. CONCLUSION: Antitoxic activityofplant extracts against CT is perspective for the development of preparations possessing inhibition effect.