RESUMO
Natural extracts used in fine fragrances (alcoholic perfumes) are rare and precious. As such, they represent an interesting target for fraudulent practices called adulterations. Absolutes, important materials used in the creation of perfumes, are obtained by organic solvent extraction of raw plant materials. Because the nonvolatile part of these natural extracts is not normalized and scarcely reported, highlighting potential adulterations present in this fraction appears highly challenging. For the first time, we investigated the use of nontargeted UHPLC-ToFMS metabolomics for this purpose, considering Viola odorata l., a plant largely used in the perfume industry, as a model. Significant differences in the metabolic fingerprints of the violet leaf absolutes were evidenced according to geographical locations, and/or adulterations. Additionally, markers of the geographical origin were detected through their molecular weight/most probable molecular formula and retention time, while adulterations were statistically validated. In this study, we thus clearly demonstrated the efficiency of UHPLC-ToFMS-based metabolomics in accelerating both the identification of the origin of raw materials as well as the search for potential adulterations in absolutes, natural products of high added value.
Assuntos
Produtos Biológicos/isolamento & purificação , Produtos Biológicos/metabolismo , Aromatizantes/metabolismo , Metabolômica , Perfumes/química , Extratos Vegetais/metabolismo , Folhas de Planta/química , Viola/química , Produtos Biológicos/química , Cromatografia Líquida de Alta Pressão , Aromatizantes/química , Aromatizantes/isolamento & purificação , Espectrometria de Massas , Conformação Molecular , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Folhas de Planta/metabolismo , Spinacia oleracea/química , Spinacia oleracea/metabolismo , Viola/metabolismoRESUMO
The metabo-ring initiative brought together five nuclear magnetic resonance instruments (NMR) and 11 different mass spectrometers with the objective of assessing the reliability of untargeted metabolomics approaches in obtaining comparable metabolomics profiles. This was estimated by measuring the proportion of common spectral information extracted from the different LCMS and NMR platforms. Biological samples obtained from 2 different conditions were analysed by the partners using their own in-house protocols. Test #1 examined urine samples from adult volunteers either spiked or not spiked with 32 metabolite standards. Test #2 involved a low biological contrast situation comparing the plasma of rats fed a diet either supplemented or not with vitamin D. The spectral information from each instrument was assembled into separate statistical blocks. Correlations between blocks (e.g., instruments) were examined (RV coefficients) along with the structure of the common spectral information (common components and specific weights analysis). In addition, in Test #1, an outlier individual was blindly introduced, and its identification by the various platforms was evaluated. Despite large differences in the number of spectral features produced after post-processing and the heterogeneity of the analytical conditions and the data treatment, the spectral information both within (NMR and LCMS) and across methods (NMR vs. LCMS) was highly convergent (from 64 to 91 % on average). No effect of the LCMS instrumentation (TOF, QTOF, LTQ-Orbitrap) was noted. The outlier individual was best detected and characterised by LCMS instruments. In conclusion, untargeted metabolomics analyses report consistent information within and across instruments of various technologies, even without prior standardisation.
RESUMO
Cyclic fatty acid monomers (CFAM) are mainly formed during heat treatments, such as frying, of edible oils. These fatty acids are mixtures of disubstituted five- or six-carbon-membered ring structures. Some earlier studies have suggested that some of these molecules could be metabolized and detoxified, but so far, neither the detoxification mechanisms nor the metabolite identifications have been elucidated. The objective of the present study was to identify the metabolites resulting from the metabolism and detoxification of CFAM. A deuterium-labeled CFAM, [9-(2)H]-10-(6-propyl-2-cyclohexenyl)-dodecenoic acid, was synthesized and fed to rats for 3 days, along with a standard chow diet while the control group was fed the same chow diet which did not contain any CFAM. Biological fluids (urine, blood) were collected for both groups of rats and analyzed using an untargeted metabolomic approach by ultra-performance liquid chromatography coupled with mass spectrometry. Two discriminant metabolites and 18 molecules derived from CFAM were identified or tentatively identified in plasma and urine samples, respectively. The structures of the metabolites suggest that CFAM having a six-carbon-membered ring could be detoxified by the classical drug metabolic pathway (phase I and phase II reactions), but our study also indicates that these are substrates for the ß-oxidation pathway and eliminated as glucuronide, sulphate, and/or nitrate conjugates. Urine metabolomics investigations without diet effects have indicated a higher excretion of medium-chain acylcarnitines in the D-CFAM diet group, which may indicate an incomplete ß-oxidation.
Assuntos
Gorduras Insaturadas na Dieta/metabolismo , Ácidos Graxos/metabolismo , Animais , Culinária , Ciclização , Gorduras Insaturadas na Dieta/análise , Gorduras Insaturadas na Dieta/sangue , Gorduras Insaturadas na Dieta/urina , Ácidos Graxos/análise , Ácidos Graxos/sangue , Ácidos Graxos/urina , Temperatura Alta , Masculino , Espectrometria de Massas , Redes e Vias Metabólicas , Metabolômica , Oxirredução , Ratos , Ratos Sprague-DawleyRESUMO
Coffee contains various bioactives implicated with human health and disease risk. To accurately assess the effects of overall consumption upon health and disease, individual intake must be measured in large epidemiological studies. Metabolomics has emerged as a powerful approach to discover biomarkers of intake for a large range of foods. Here we report the profiling of the urinary metabolome of cohort study subjects to search for new biomarkers of coffee intake. Using repeated 24-hour dietary records and a food frequency questionnaire, 20 high coffee consumers (183-540 mL/d) and 19 low consumers were selected from the French SU.VI.MAX2 cohort. Morning spot urine samples from each subject were profiled by high-resolution mass spectrometry. Partial least-square discriminant analysis of multidimensional liquid chromatography-mass spectrometry data clearly distinguished high consumers from low via 132 significant (p-value<0.05) discriminating features. Ion clusters whose intensities were most elevated in the high consumers were annotated using online and in-house databases and their identities checked using commercial standards and MS-MS fragmentation. The best discriminants, and thus potential markers of coffee consumption, were the glucuronide of the diterpenoid atractyligenin, the diketopiperazine cyclo(isoleucyl-prolyl), and the alkaloid trigonelline. Some caffeine metabolites, such as 1-methylxanthine, were also among the discriminants, however caffeine may be consumed from other sources and its metabolism is subject to inter-individual variation. Receiver operating characteristics curve analysis showed that the biomarkers identified could be used effectively in combination for increased sensitivity and specificity. Once validated in other cohorts or intervention studies, these specific single or combined biomarkers will become a valuable alternative to assessment of coffee intake by dietary survey and finally lead to a better understanding of the health implications of coffee consumption.
Assuntos
Café/metabolismo , Metaboloma , Biomarcadores/urina , Estudos de Coortes , Dieta , Análise Discriminante , Humanos , Metabolômica , Urinálise , Xantinas/metabolismo , Xantinas/urinaRESUMO
Foods of plant origin contain a large number of phytochemicals that may positively affect health. Phytochemicals are largely excreted in urine as metabolites that are formed in host tissues or by the microbiota and constitute a great proportion of the urinary metabolome. The latter can be characterized by a metabolomics approach. In this work, we compared the metabolism of lignins to that of the structurally related ferulic acid (FA) and sinapic acid (SA). Five groups of rats (n = 5) were fed for 2 d a purified diet alone [control (C)] or supplemented with lignin-enriched wheat bran (3% of the diet, wt:wt), poplar wood lignins (0.42%), FA (0.42%), or SA (0.42%). The metabolomes of urine samples collected after 1 and 2 d of supplementation were analyzed by high-resolution MS (liquid chromatography/quadrupole time-of-flight). Comparing metabolic fingerprints by gathering semiquantitative information on several hundreds of metabolites and using multivariate statistical analysis (partial least squares for discriminant analysis) showed the similarity between both lignin-supplemented and C groups and confirmed that lignins are largely inert and not absorbed in the body. One the other hand, metabolic fingerprints of the 2 phenolic acid-supplemented groups were clearly distinct from the C group. Differences between the groups were mainly from nonmetabolized FA and SA and metabolites excreted in urine. Thirteen of them were identified as sulfate esters and glucuronide and glycine conjugates of the same phenolic acids, and of dihydrosinapic, vanillic, and benzoic acids. This study shows that metabolomics allows the identification of new metabolites of phytochemicals and can be used to distinguish individuals fed different phytochemical-containing foods.
Assuntos
Ácidos Cumáricos/metabolismo , Lignina/metabolismo , Animais , Ácidos Cumáricos/urina , Dieta , Flavonoides/metabolismo , Flavonoides/urina , Lignina/urina , Masculino , Metabolismo , Fenóis/metabolismo , Fenóis/urina , Extratos Vegetais/química , Extratos Vegetais/metabolismo , Extratos Vegetais/urina , Polifenóis , Ratos , Ratos WistarRESUMO
Unbalanced diets generate oxidative stress commonly associated with the development of diabetes, atherosclerosis, obesity and cancer. Dietary flavonoids have antioxidant properties and may limit this stress and reduce the risk of these diseases. We used a metabolomic approach to study the influence of catechin, a common flavonoid naturally occurring in various fruits, wine or chocolate, on the metabolic changes induced by hyperlipidemic diets. Male Wistar rats ( n = 8/group) were fed during 6 weeks normolipidemic (5% w/w) or hyperlipidemic (15 and 25%) diets with or without catechin supplementation (0.2% w/w). Urines were collected at days 17 and 38 and analyzed by reverse-phase liquid chromatography-mass spectrometry (LC-QTOF). Hyperlipidic diets led to a significant increase of oxidative stress in liver and aorta, upon which catechin had no effect. Multivariate analyses (PCA and PLS-DA) of the urine fingerprints allowed discrimination of the different diets. Variables were then classified according to their dependence on lipid and catechin intake (ANOVA). Nine variables were identified as catechin metabolites of tissular or microbial origin. Around 1000 variables were significantly affected by the lipid content of the diet, and 76 were fully reversed by catechin supplementation. Four variables showing an increase in urinary excretion in rats fed the high-fat diets were identified as deoxycytidine, nicotinic acid, dihydroxyquinoline and pipecolinic acid. After catechin supplementation, the excretion of nicotinic acid was fully restored to the level found in the rats fed the low-fat diet. The physiological significance of these metabolic changes is discussed.
Assuntos
Aorta/metabolismo , Catequina/farmacologia , Gorduras na Dieta/farmacologia , Fígado/metabolismo , Espectrometria de Massas/métodos , Animais , Antioxidantes/metabolismo , Aorta/efeitos dos fármacos , Peso Corporal/efeitos dos fármacos , Catequina/metabolismo , Catequina/urina , Colesterol/sangue , Cromatografia Líquida de Alta Pressão/métodos , Desoxicitidina/metabolismo , Desoxicitidina/urina , Ingestão de Alimentos/efeitos dos fármacos , Glutationa/metabolismo , Glutationa Peroxidase/metabolismo , Glutationa Transferase/metabolismo , Fígado/efeitos dos fármacos , Fígado/enzimologia , Masculino , Malondialdeído/metabolismo , Malondialdeído/urina , Análise Multivariada , Niacina/metabolismo , Niacina/urina , Ácidos Pipecólicos/metabolismo , Ácidos Pipecólicos/urina , Quinolinas/metabolismo , Quinolinas/urina , Ratos , Ratos Wistar , Triglicerídeos/sangueRESUMO
BACKGROUND: Oxidative stress likely constitutes an important contributing factor in the onset of degenerative diseases associated with folate deficiency. Direct, as well as homocysteine-linked, antioxidant properties of folate could explain its preventive effect on these pathologies. AIM OF THE STUDY: Our study aimed at determining the changes in the redox status of adult rats as a function of folate intake. METHODS: Adult male rats were pair-fed for 4 weeks with a semi-synthetic diet containing 0, 0.5, 1.5, 8 or 20 mg of folic acid/kg. Folate and homocysteine concentrations, redox status markers and antioxidant enzyme activities were measured in the plasma and/or liver of the rats. A principal component analysis of the overall data was performed to draw a general scheme of the changes observed between the conditions. RESULTS: Folate deficiency caused increased homocysteinemia and features of oxidative stress including reduced plasma antioxidant capacity together with increased lipid peroxidation in liver and heart. This was associated with an increase in the specific activity of several enzymes involved in liver glutathione metabolism (glutathione peroxidase, glutathione reductase and glutathione S-transferase), suggesting an adaptive tissue response to the oxidative stress induced by folate deficiency. In contrast, no such variation was observed for hepatic superoxide dismutase and catalase. CONCLUSION: Despite no changes in hepatic levels of total glutathione, our findings indicate that glutathione-dependent antioxidant pathways could be particularly involved in the compensatory mechanism committed by liver to counteract the oxidative stress induced by folate deficiency. They also suggest that folate supplementation may not be associated with a better antioxidant protection of rats.