RESUMO
Resolution of bacterial infections is often hampered by both resistance to conventional antibiotic therapy and hiding of bacterial cells inside biofilms, warranting the development of innovative therapeutic strategies. Here, we report the efficacy of blue laser light in eradicating Pseudomonas aeruginosa cells, grown in planktonic state, agar plates and mature biofilms, both in vitro and in vivo, with minimal toxicity to mammalian cells and tissues. Results obtained using knock-out mutants point to oxidative stress as a relevant mechanism by which blue laser light exerts its anti-microbial effect. Finally, the therapeutic potential is confirmed in a mouse model of skin wound infection. Collectively, these data set blue laser phototherapy as an innovative approach to inhibit bacterial growth and biofilm formation, and thus as a realistic treatment option for superinfected wounds.
Assuntos
Biofilmes/crescimento & desenvolvimento , Biofilmes/efeitos da radiação , Lasers , Luz , Estresse Oxidativo , Pseudomonas aeruginosa/crescimento & desenvolvimento , Pseudomonas aeruginosa/efeitos da radiação , Animais , Linhagem Celular , Meios de Cultura , Modelos Animais de Doenças , Humanos , Camundongos Endogâmicos C57BL , Modelos Biológicos , Infecções por Pseudomonas/terapia , Radioterapia/métodos , Resultado do Tratamento , Infecção dos Ferimentos/terapiaAssuntos
Fator Neurotrófico Derivado do Encéfalo/sangue , Encéfalo/fisiologia , Disfunção Cognitiva/psicologia , Música/psicologia , Plasticidade Neuronal , Análise e Desempenho de Tarefas , Idoso , Idoso de 80 Anos ou mais , Disfunção Cognitiva/fisiopatologia , Feminino , Humanos , Hidrocortisona/sangue , Masculino , Musicoterapia , Desempenho Psicomotor , Comportamento EspacialRESUMO
CONCLUSION: The study suggests that Mozart therapy could be a valid alternative to the common sound therapy methods in tinnitus patients. OBJECTIVES: The aim of the study was to evaluate the presence of the Mozart effect as indexed by a variation in tinnitus intensity and tolerability. METHOD: Sixty-two individuals aged between 22 and 78 years, reporting tinnitus for at least 1 year, were enrolled for the study. All patients attended a 1 h cognitive behavioral counseling session and listened to Mozart's sonata k448 for 1 h per day for a month. Afterwards patients listened to Beethoven's Für Elise sonata for 1 h per day for a month. To evaluate the general stress level, the impact of tinnitus on patients' quality of life, and the intensity of tinnitus, patients were invited to participate in three tests: the Measure du Stress Psychologique (MSP) questionnaire, the Tinnitus Handicap Inventory (THI), and a 0 to 10 visual analog scale (VAS). RESULTS: For all the parameters investigated, MSP, THI, and intensity, there was a general significant improvement between the pre- and post-listening evaluation. A significant improvement, as regards THI and intensity, could already be appreciated after a single exposure to Mozart's sonata.