Audição musical para alívio da ansiedade do acompanhante pediátrico / Audición musical para alivio de la ansiedad del acompañante pediátrico / Musical listening for anxiety relief in pediatric companions

Objetivo investigar os efeitos da audição musical sobre os níveis de ansiedade pós-operatória de acompanhantes de crianças submetidas a cirurgias em comparação ao cuidado convencional de uma clínica cirúrgica pediátrica. Método estudo quase-experimental realizado em um hospital público de ensino com 62 acompanhantes de crianças em pós-operatório. O grupo controle - GC (n=32) foi submetido aos cuidados convencionais da unidade e o grupo experimental - GE (n=30) foi submetido à audição musical. Ambos os grupos tiveram os indicadores fisiológicos aferidos, nível de ansiedade mensurado por meio do Inventário de Ansiedade Traço-Estado e responderam um questionário estruturado para caracterização da amostra. Resultados GE apresentou médias mais elevadas e estatisticamente significativas nos itens do Inventário de Ansiedade Traço-Estado sentir-se "à vontade", "descansado", "descontraído" e "satisfeito" em relação ao GC, após a audição musical. Conclusão a audição musical tem o potencial de reduzir o nível de ansiedade pós-operatória dos acompanhantes de crianças submetidas a cirurgia.

Objetivo investigar los efectos de la audición musical sobre niveles de ansiedad postoperatoria de acompañantes de niños sometidos a cirugías, en comparación a atención convencional de una clínica quirúrgica pediátrica. Método estudio cuasiexperimental, en hospital público de enseñanza, con 62 acompañantes de niños en postoperatorio. El grupo control GC (n=32) fue sometido a los cuidados convencionales de la unidad y el grupo experimental GE (n=30) a la audición musical. Ambos grupos tuvieron los indicadores fisiológicos evaluados, nivel de ansiedad medido por medio del State-Trait Anxiety Inventory y respondieron a cuestionario estructurado para caracterización de la muestra. Resultados GE presentó medias más elevadas y estadísticamente significativas en los ítems del State-Trait Anxiety Inventory sentirse "a voluntad", "descansado", "relajado" y "satisfecho" en relación al GC, después de la audición musical. Conclusión la audición musical tiene potencial de reducir el nivel de ansiedad postoperatoria de acompañantes de niños sometidos a cirugía.

Objective to investigate the effects of music listening on the level of postoperative anxiety of companions of children submitted to surgeries in comparison with the conventional care of a pediatric surgical clinic. Methodology a quasi-experimental study conducted in a public teaching hospital with 62 companions of children in the postoperative period. The control group - CG (n=32) was submitted to the clinic's conventional care and the experimental group - EG (n=30) was submitted to music listening. Both groups had their physiological indicators measured, level of anxiety measured by the State-Trait Anxiety Inventory (STAI), and they answered a structured questionnaire for sample characterization. Results the EG presented higher and statistically significant mean values in the STAI items: feel 'at ease,' 'well-rested,' 'relaxed,' and 'satisfied' when compared to the CG after music listening. Conclusion music listening may reduce the level of postoperative anxiety of companions of children submitted to surgery.

Structural basis for targeting the chromatin repressor Sfmbt to Polycomb response elements.

Polycomb group (PcG) protein complexes repress developmental regulator genes by modifying their chromatin. How different PcG proteins assemble into complexes and are recruited to their target genes is poorly understood. Here, we report the crystal structure of the core of the Drosophila PcG protein complex Pleiohomeotic (Pho)-repressive complex (PhoRC), which contains the Polycomb response element (PRE)-binding protein Pho and Sfmbt. The spacer region of Pho, separated from the DNA-binding domain by a long flexible linker, forms a tight complex with the four malignant brain tumor (4MBT) domain of Sfmbt. The highly conserved spacer region of the human Pho ortholog YY1 binds three of the four human 4MBT domain proteins in an analogous manner but with lower affinity. Comparison of the Drosophila Pho:Sfmbt and human YY1:MBTD1 complex structures provides a molecular explanation for the lower affinity of YY1 for human 4MBT domain proteins. Structure-guided mutations that disrupt the interaction between Pho and Sfmbt abolish formation of a ternary Sfmbt:Pho:DNA complex in vitro and repression of developmental regulator genes in Drosophila. PRE tethering of Sfmbt by Pho is therefore essential for Polycomb repression in Drosophila. Our results support a model where DNA tethering of Sfmbt by Pho and multivalent interactions of Sfmbt with histone modifications and other PcG proteins create a hub for PcG protein complex assembly at PREs.