RESUMO
Technologically promising microbe association, consisting of aerobic and anaerobic sporulating bacteria has been isolated. The association synthesizes molecular hydrogen during fermentation of potato and starch. The association was isolated from soil, pasteurized on the boiling water bath. The association destroys potato during 5-7 days with a decrease of mass up to 17.4 times and synthesizes gas consisting of 60% of H2.
Assuntos
Bactérias Aeróbias/crescimento & desenvolvimento , Bactérias Anaeróbias/crescimento & desenvolvimento , Hidrogênio/isolamento & purificação , Bactérias Aeróbias/metabolismo , Bactérias Aeróbias/fisiologia , Bactérias Anaeróbias/metabolismo , Bactérias Anaeróbias/fisiologia , Biocombustíveis , Fermentação , Hidrogênio/metabolismo , Solanum tuberosum/química , Esporos BacterianosRESUMO
The hybrid plants with transformed plastids were regenerated after PEG fusion of chlorophyll-deficient Lycopersicon peruvianum leaf mesophyll protoplasts and leaf mesophyll protoplasts of Solanum rickii, which were previously genetically transformed and as the result were resistant to streptomycine and spectinomycine. The hybrid callus selection was based on the inability of the Lycopersicon peruvianum minicalli to have the green coloration and on the inability of gamma-preirradiated Solanum rickii protoplasts to divide. The hybrids were identified on the base of PCR analyses of nuclear and plastid DNA.
Assuntos
Cloroplastos/genética , Cloroplastos/fisiologia , Células Híbridas , Regeneração/fisiologia , Solanum lycopersicum/genética , Solanum/genética , Transformação Genética , Fusão Celular/métodos , Núcleo Celular/química , Cloroplastos/química , Cloroplastos/efeitos da radiação , DNA/metabolismo , Raios gama , Mutação , Reação em Cadeia da Polimerase , Seleção GenéticaRESUMO
The biolistic method was used for genetic transformation of Solanum rickii chloroplasts with aadA gene encoding resistance to streptomycine and spectinomycine. Selective pressure was applied immediately after microbombardment to avoid appearance of mutant lines. The transplastomic Solanum rickii plants remained green during two years cultivation on the media supplemented with two antibiotics. There were no morphological differences between the transformed and the wild type plants.