RESUMO
OBJECTIVE: The aim of our study was to determine the diagnostic usefulness of a newly developed basophil activation test (BAT) in patients allergic to Dermatophagoides pteronyssinus and pollens. We also analyzed the influence of cetirizine on CD63 upregulation. This popular antihistamine strongly inhibits skin tests, but its impact on BAT sensitivity remains unknown and deserves at least preliminary determination. METHODS: The study sample comprised 22 patients allergic to house dust mite and pollens and 19 healthy controls. All participants underwent skin prick testing and the newly developed flow-cytometric basophil activation test. The protocol for allergen-induced basophil CD63 upregulation consisted of whole blood samples that were processed and stained with anti-CCR3/CD63 antibodies added to the buffer at the beginning of stimulation. Skin prick tests and BAT were performed twice--before and 2 hours after ingestion of 10 mg of cetirizine. RESULTS: The new BAT is characterized by its short processing time, easy basophil gating, and strong CD63 upregulation with very high sensitivity and excellent specificity. Our results suggest that allergen-induced CD63 upregulation by higher doses of allergens is not inhibited 2 hours after administration of cetirizine (unlike skin prick tests). CONCLUSION: The BAT is a very useful and precise method for the diagnosis of allergy to aeroallergens. It is not influenced by cetirizine.
Assuntos
Antígenos CD/metabolismo , Teste de Degranulação de Basófilos/métodos , Basófilos/metabolismo , Hipersensibilidade/diagnóstico , Glicoproteínas da Membrana de Plaquetas/metabolismo , Adulto , Animais , Antígenos CD/genética , Antígenos CD/imunologia , Antígenos de Dermatophagoides/imunologia , Antígenos de Plantas/efeitos adversos , Antígenos de Plantas/imunologia , Basófilos/efeitos dos fármacos , Basófilos/imunologia , Basófilos/patologia , Degranulação Celular/efeitos dos fármacos , Degranulação Celular/imunologia , Separação Celular , Células Cultivadas , Cetirizina/administração & dosagem , Cetirizina/farmacologia , Dermatophagoides pteronyssinus/imunologia , Estudos de Viabilidade , Feminino , Citometria de Fluxo , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/imunologia , Antagonistas não Sedativos dos Receptores H1 da Histamina/administração & dosagem , Antagonistas não Sedativos dos Receptores H1 da Histamina/farmacologia , Humanos , Hipersensibilidade/tratamento farmacológico , Hipersensibilidade/imunologia , Hipersensibilidade/patologia , Masculino , Glicoproteínas da Membrana de Plaquetas/genética , Glicoproteínas da Membrana de Plaquetas/imunologia , Pólen/efeitos adversos , Pólen/imunologia , Sensibilidade e Especificidade , Tetraspanina 30RESUMO
Basophil spontaneous histamine release test was performed in seventeen patients suffered from seasonal allergic rhinitis before, 2 weeks after immunotherapy and during pollen season. During pollen season at the same day non-specific bronchial hyperreactivity to histamine was estimated. Values of spontaneous basophil histamine release before immunotherapy were not different from control group but significantly increased after immunotherapy and during pollen season. There was no correlation between degree of nonspecific bronchial hyperreactivity and values of spontaneous histamine release from basophils.
Assuntos
Alérgenos/uso terapêutico , Basófilos/imunologia , Hiper-Reatividade Brônquica/terapia , Glutaral/uso terapêutico , Liberação de Histamina/efeitos dos fármacos , Extratos Vegetais/uso terapêutico , Pólen , Rinite Alérgica Sazonal/terapia , Tirosina/uso terapêutico , Antígenos de Plantas/uso terapêutico , Hiper-Reatividade Brônquica/imunologia , Combinação de Medicamentos , Humanos , Imunoterapia , Distribuição Normal , Rinite Alérgica Sazonal/imunologiaRESUMO
Twenty six patients with rhinitis were desensitized with Pollinex. Circulating immune complexes were determined in these patients before and after immunotherapy. Two assay techniques - precipitation and PEG-CCT were applied to assay circulating immune complexes. It was found that specific immunotherapy with Pollinex does not produce circulating immune complexes in patients with hay fever.