RESUMO
<p><b>OBJECTIVE</b>To investigate the effects of Haikun Shenxi on the expression of platelet-derived growth factor-BB (PDGF-BB) and mRNA in renal tissue of rats with adriamycin nephropathy.</p><p><b>METHOD</b>Rat model was established by unilateral nephrectomy and injecting adriamycin intraperitoneally. The adriamycin-induced nephrotic rats were randomly divided into 6 groups: normal group, sham operation group, model group, lotensin treatment group, Haikun Shenxi low and high dose treatment groups (0.77, 0.08 mg x kg(-1). Ten weeks later, the 24 hour urine protein and blood biochemistry examinations and renal pathologic changes were observed, and the expression of PDGF-BB and mRNA was measured using immunohistochemical method.</p><p><b>RESULT</b>Compared with model group, proteinuria and the levels of serum creatinine (Scr) , urea nitrogen (BUN) were decreased obviously in both Haikun Shenxi low and high dose groups. The expression of PDGF-BB and mRNA was mostly presented in cytoplasm of renal tubular epithelial cells and mesangial area, and it could be reduced significantly after treatment (P < 0. 05).</p><p><b>CONCLUSION</b>The level of PDGF-BB and mRNA is high in renal tissue of adriamycin-induced nephrotic rats. This progress could be effectively inhibited by Haikun Shenxi and the mechanism may be that it can control the excessive expression of PDGF-BB and mRNA.</p>
Assuntos
Animais , Masculino , Ratos , Nitrogênio da Ureia Sanguínea , Creatinina , Sangue , Doxorrubicina , Medicamentos de Ervas Chinesas , Química , Farmacologia , Regulação da Expressão Gênica , Mesângio Glomerular , Metabolismo , Patologia , Glomerulosclerose Segmentar e Focal , Genética , Metabolismo , Imuno-Histoquímica , Hibridização In Situ , Rim , Metabolismo , Patologia , Medicina Tradicional Chinesa , Phaeophyceae , Química , Fator de Crescimento Derivado de Plaquetas , Genética , Polissacarídeos , Química , Farmacologia , Proteínas Proto-Oncogênicas c-sis , RNA Mensageiro , Genética , Distribuição Aleatória , Ratos WistarRESUMO
<p><b>OBJECTIVE</b>To study the influence of compound Biejia Ruangan Prescription (CBRP) on extracelluar matrix in bleomycin induced pulmonary fibrosis rats.</p><p><b>METHOD</b>54 male Sprague-Dawley rats were randomly divided into 6 groups (9 rats in each group). Rats in the model control group, positive medicine group, and high, moderate and low CBRP groups were injected with bleomycin A5 by trachea, and rats in sham-model control group with same volume normal saline. 29 days after the injection, CBRP solution of different dosages (1.4 g x kg(-1), 0.7 g x kg(-1), 0.35 g x kg(-1)) was respectively given to rats in the high, moderate and low CBRP group by gavage, while equal volume of normal saline was given to those in the sham-model control group and model control group, and an equal volume of prednisone (0.56 mg x kg(-1)) was given to those in positive medicine control group. On the 80th day, the levels of III-collagen, IV-collagen, laminin and hyaluronic acid in the serum were determined, the determination of hydroxyproline in lung homogenates was analyzed, and the right lung was incised to make pathological sections which were stained with Hematoxylin-Eosin (HE) and Masson staining for pathological diagnosis.</p><p><b>RESULT</b>CBRP could decrease the levels of III-collagen, IV-collagen, laminin and hyaluronic acid in the serum.</p><p><b>CONCLUSION</b>CBRP may play its therapeutic role by leveling down the content of extracellular matrix in rats with pulmonary fibrosis induced by Bleomycin A5.</p>