Magnetic Micellar Nanovehicles: Prospects of Multifunctional Hybrid Systems for Precision Theranostics.

Hybrid nanoarchitectures such as magnetic polymeric micelles (MPMs) are among the most promising nanotechnology-enabled materials for biomedical applications combining the benefits of polymeric micelles and magnetic nanoparticles within a single bioinstructive system. MPMs are formed by the self-assembly of polymer amphiphiles above the critical micelle concentration, generating a colloidal structure with a hydrophobic core and a hydrophilic shell incorporating magnetic particles (MNPs) in one of the segments. MPMs have been investigated most prominently as contrast agents for magnetic resonance imaging (MRI), as heat generators in hyperthermia treatments, and as magnetic-susceptible nanocarriers for the delivery and release of therapeutic agents. The versatility of MPMs constitutes a powerful route to ultrasensitive, precise, and multifunctional diagnostic and therapeutic vehicles for the treatment of a wide range of pathologies. Although MPMs have been significantly explored for MRI and cancer therapy, MPMs are multipurpose functional units, widening their applicability into less expected fields of research such as bioengineering and regenerative medicine. Herein, we aim to review published reports of the last five years about MPMs concerning their structure and fabrication methods as well as their current and foreseen expectations for advanced biomedical applications.

Nanostructuring lipid carriers using Ridolfia segetum (L.) Moris essential oil.

The therapeutic potential of essential oils is widely recognized since antiquity, due to their antibacterial, antifungal, anti-inflammatory and immuno-modulatory properties. In particular, their physicochemical characteristics, such as lipophilicity and permeation enhancement effect have sparked attention for the development of innovative lipid nanosystems. The present work aimed at developing a differentiated nanostructured lipid carrier (NLC) based formulation for topical application, using the Ridolfia segetum essential oil (REO), isolated by hydrodistillation from this Portuguese aromatic plant, with a dual key function, as active and simultaneously nanostructuring component of the nanoparticles. The incorporation of the essential oil in the solid lipid matrix, followed by the respective hot high-pressure homogenization, led to particles with a size of 143 ±â€¯5 nm, along with a polydispersity index of 0.21, a zeta potential of -16.3 ±â€¯0.6 mV, encapsulation efficacy of ca. 100% and loading capacity of 1.4%. A comprehensive physicochemical characterization of the lipid nanosystem, including morphology, structural, thermal and accelerated stability analysis confirmed its nanostructured nature. REO-NLC was further jellified for designing an appropriate semisolid topical dosage form. In vitro release, permeation and skin retention studies evidenced a sustained release behaviour and a reservoir-like effect, suitable for a prolonged topical delivery. Cytotoxicity studies, performed in fibroblasts and keratinocytes, revealed the biocompatibility of the developed formulations. This work highlights the critical role of REO as a multiaddressable compound, both as active pharmaceutical ingredient and nanostructuring agent, able to tailor the permeation enhancement profile of nanoparticles towards topical delivery purposes and concomitantly presenting a safety profile for cosmetic and/or pharmaceutical purposes.

Bioequivalence of topical generic products. Part 1: Where are we now?

Regulatory accepted methods for bioequivalence assessment of topical generic products generally involve long and expensive clinical endpoint studies. The only alternative relies on pharmacodynamic trials, solely applicable to corticosteroids. Considerable efforts have been channeled towards the development and validation of other analytical surrogates. The majority of these alternative methods rely on in vitro methodologies that allow a more sensitive and reproducible bioequivalence assessment, avoiding at the same time the financial burden that deeply characterizes clinical trials. The development and validation of these methods represent interesting areas of opportunities for generic drugs, since by enabling faster submission and approval processes, an enlargement of topical drug products with generic version is more easily attainable. This review aims to present a critical discussion of the most promising alternative methods, with particular emphasis on in vitro permeation studies and near infrared spectroscopy studies. Since the last technique is not broadly forecast as a bioequivalence assessment tool, its suitability is assessed by a careful analysis of patents that claim the use of NIR radiation in the skin. In fact, the extensive coverage of the devices that use this technology highlights its applicability towards a better understanding of the mechanism underlying topical drug delivery.

[Inclusion of traditional birth attendants in the public health care system in Brazil: reflecting on challenges]. / Inclusão de parteiras tradicionais no Sistema Único de Saúde no Brasil: reflexão sobre desafios.

The present article describes an experience with traditional birth attendants carried out in the state of Tocantins, Brazil, between 2010 and 2014. The experience was part of a diagnostic project to survey home deliveries in the state of Tocantins and set up a registry of traditional birth attendants for the Health Ministry's Working with Traditional Birth Attendants Program (PTPT). The project aimed to articulate the home deliveries performed by traditional birth attendants to the local health care systems (SUS). Sixty-seven active traditional birth attendants were identified in the state of Tocantins, and 41 (39 indigenous) participated in workshops. During these workshops, they discussed their realities, difficulties, and solutions in the context of daily adversities. Birth attendants were also trained in the use of biomedical tools and neonatal resuscitation. Based on these experiences, the question came up regarding the true effectiveness of the strategy to include traditional birth attendants in the SUS. The present article discusses this theme with support from the relevant literature. The dearth of systematic studies focusing on the impact of PTPT actions on the routine of traditional birth attendants, including perinatal outcomes and remodeling of health practices in rural, riverfront, former slave, forest, and indigenous communities, translates into a major gap in terms of the knowledge regarding the effectiveness of such initiatives.

Inclusão de parteiras tradicionais no Sistema Único de Saúde no Brasil: reflexão sobre desafios / Inclusion of traditional birth attendants in the public health care system in Brazil: reflecting on challenges

O presente artigo apresenta uma experiência de trabalho com parteiras tradicionais realizada no estado do Tocantins, Brasil, entre 2010 e 2014, no âmbito do Projeto Diagnóstico da Situação do Parto Domiciliar no Tocantins e Cadastramento de Parteiras Tradicionais, vinculado ao Programa Trabalhando com Parteiras Tradicionais (PTPT), do Ministério da Saúde. O projeto objetivou uma articulação entre o parto e o nascimento domiciliares assistidos por parteiras tradicionais e os sistemas locais de saúde. No estado, identificaram-se 67 parteiras atuantes. Durante oficinas de capacitação, 41 parteiras tradicionais, dentre as quais 39 indígenas, discutiram suas realidades, dificuldades e soluções frente a um cotidiano com muitas adversidades. Essas parteiras foram ainda capacitadas no uso de instrumentos biomédicos e na condução da reanimação neonatal. A partir dessas experiências, surgiu o questionamento sobre a real efetividade da estratégia para incluir as parteiras tradicionais no SUS. O presente artigo discute esse tema com apoio na literatura pertinente. A carência de estudos sistemáticos acerca do impacto das ações do PTPT no cotidiano das parteiras, incluindo os desfechos perinatais e o remodelamento das práticas de saúde em populações rurais, ribeirinhas, quilombolas, de floresta e indígenas, implica lacunas importantes no que diz respeito à efetividade desse tipo de iniciativa.

The present article describes an experience with traditional birth attendants carried out in the state of Tocantins, Brazil, between 2010 and 2014. The experience was part of a diagnostic project to survey home deliveries in the state of Tocantins and set up a registry of traditional birth attendants for the Health Ministry's Working with Traditional Birth Attendants Program (PTPT). The project aimed to articulate the home deliveries performed by traditional birth attendants to the local health care systems (SUS). Sixty-seven active traditional birth attendants were identified in the state of Tocantins, and 41 (39 indigenous) participated in workshops. During these workshops, they discussed their realities, difficulties, and solutions in the context of daily adversities. Birth attendants were also trained in the use of biomedical tools and neonatal resuscitation. Based on these experiences, the question came up regarding the true effectiveness of the strategy to include traditional birth attendants in the SUS. The present article discusses this theme with support from the relevant literature. The dearth of systematic studies focusing on the impact of PTPT actions on the routine of traditional birth attendants, including perinatal outcomes and remodeling of health practices in rural, riverfront, former slave, forest, and indigenous communities, translates into a major gap in terms of the knowledge regarding the effectiveness of such initiatives.