Preliminary Phytochemical and Biological Evaluation of Rudbeckia hirta Flowers.

Black-eyed Susan (Rudbeckia hirta L.), a flowering plant with various traditional medicinal uses, has recently garnered interest for its therapeutic properties. However, little is known about the potential therapeutic activities of the plant species. The current study focused on conducting a comprehensive investigation into the chemical composition and bioactivity of black-eyed Susan cultivated in Romania. Untargeted metabolite profiling and UHPLC-HR-MS phytochemical analysis of the studied extract revealed the presence of more than 250 compounds pertaining to different classes, including sesquiterpene lactones, polyphenolic acids, flavonoids, amino acids, and fatty acids. The tested extract exhibited inhibitory activity against Gram-positive bacteria and showed promising antifungal activity. It also demonstrated potent antioxidant properties through iron chelation and 15-LOX inhibition capacities, as well as inhibition of cell growth, particularly on the MCF-7 cell line, suggesting potential anticancer effects. Therefore, current research provides valuable information on the antioxidant, antimicrobial, and antitumor potential of Rudbeckia hirta flowers. Implicitly, the discovery of such a wide range of biosubstances, together with the biological activity observed for the studied extract in these preliminary in vitro studies, paves the way for future investigation of the potential application of the plant in the pharmaceutical and nutraceutical sectors.

In vitro Evaluation of Antioxidant and Antibacterial Activities of Eco-friendly Synthesized Silver Nanoparticles using Quercus robur Bark Extract.

AIMS: This study reports a simple, cost-effective, and environmentally friendly method to obtain silver nanoparticles (AgNPs) using an aqueous extract of Quercus robur bark. METHODS: AgNPs synthesis conditions such as silver nitrate concentration, extract:AgNO3 volume ratio, pH, temperature, and reaction time have been examined. After optimizing the synthesis, the obtained AgNPs were characterized by different methods such as UV-Vis, TEM, EDX, and FTIR. The antioxidant activity was evaluated using lipoxygenase inhibition capacity and inhibition of erythrocyte hemolysis mediated by peroxyl free radicals tests. The antimicrobial potential of the samples was tested against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. RESULTS: The AgNPs synthesis process is influenced by reaction conditions, the optimum established values being, in this case: concentration of 3 mM AgNO3, 1:9 extract: AgNO3 volume ratio, pH value of 6, 60 ºC temperature, and 90 minutes stirring time. The shape of the synthesized AgNPs was predominantly spherical, with an average size of 50 nm. The SPR band at 432 nm, the strong EDX signal at ~ 3 keV and the zeta potential of -13.88 mV revealed the formation of AgNPs and electrostatic stabilization of the colloidal solution. FTIR analysis confirmed the participation of molecules from the extract in the synthesis and stabilization of AgNPs. The obtained nanoparticles showed improved antioxidant, antifungal and antibacterial activities compared to the extract. CONCLUSION: The results open the possibility of exploring new applications of nanoparticles obtained via green synthesis.

Neuroprotective Effect of Yucca schidigera Roezl ex Ortgies Bark Phenolic Fractions, Yuccaol B and Gloriosaol A on Scopolamine-Induced Memory Deficits in Zebrafish.

Y. schidigera contains a number of unusual polyphenols, derivatives of resveratrol and naringenin, called spiro-flavostilbenoids, which have potent in vitro anti-inflammatory, antioxidant, and moderate cholinesterase inhibitory activities. To date, these compounds have not been tested in vivo for the treatment of neurodegenerative diseases. The aim of the present study was to evaluate the effects of both single spiro-flavostilbenoids (yuccaol B and gloriosaol A) and phenolic fractions derived from Y. schidigera bark on scopolamine-induced anxiety and memory process deterioration using a Danio rerio model. Detailed phytochemical analysis of the studied fractions was carried out using different chromatographic techniques and Nuclear Magnetic Resonance (NMR). The novel tank diving test was used as a method to measure zebrafish anxiety, whereas spatial working memory function was assessed in Y-maze. In addition, acetylcholinesterase/butyrylcholinesterase (AChE/BChE) and 15-lipooxygenase (15-LOX) inhibition tests were performed in vitro. All pure compounds and fractions under study exerted anxiolytic and procognitive action. Moreover, strong anti-oxidant capacity was observed, whereas weak inhibition towards cholinesterases was found. Thus, we may conclude that the observed behavioral effects are complex and result rather from inhibition of oxidative stress processes and influence on cholinergic muscarinic receptors (both 15-LOX and scopolamine assays) than effects on cholinesterases. Y. schidigera is a source of substances with desirable properties in the prevention and treatment of neurodegenerative diseases.

Neuroprotective and Antioxidant Enhancing Properties of Selective Equisetum Extracts.

The sterile stems belonging to the Equisetum species are often used in traditional medicine of various nations, including Romanians. They are highly efficient in treating urinary tract infections, cardiovascular diseases, respiratory tract infections, and medical skin conditions due to their content of polyphenolic derivatives that have been isolated. In this regard, this study aimed to provide the chemical composition of the extracts obtained from the Equisetum species (E. pratense, E. sylvaticum, E. telmateia) and to investigate the biological action in vitro and in vivo. For the chemical characterization of the analyzed Equisetum species extracts, studies were performed by using ultra-high-performance liquid chromatography (UHPLC-DAD). In vitro evaluation of the antioxidant activity of the plant extracts obtained from these species of Equisetum genus was determined. The neuroprotective activity of these three ethanolic extracts from the Equisetum species using zebrafish tests was determined in vivo. All obtained results were statistically significant. The results indicate that E. sylvaticum extract has a significant antioxidant activity; whereas, E. pratense extract had anxiolytic and antidepressant effects significantly higher than the other two extracts used. All these determinations indicate promising results for the antioxidant in vitro tests and neuroprotective activity of in vivo tests, particularly mediated by their active principles.

Phytochemical Profile, Antioxidant Activity, and Cytotoxicity Assessment of Tagetes erecta L. Flowers.

Tagetes erecta L. is a popular ornamental plant of the Asteraceae family, which is widely cultivated not only for its decorative use, but also for the extraction of lutein. Besides carotenoid representatives, which have been extensively studied, other important classes of secondary metabolites present in the plant, such as polyphenols, could exhibit important biological activities. The phytochemical analysis of a methanolic extract obtained from T. erecta inflorescences was achieved using liquid chromatography-mass spectrometry (LC-MS) techniques. The extract was further subjected to a multistep purification process, which allowed the separation of different fractions. The total extract and its fractions contain several polyphenolic compounds, such as hydroxybenzoic and hydroxycinnamic acid derivatives, flavonols (especially quercetagetin glycosides), and several aglycons (e.g., quercetin, patuletin). One of the fractions, containing mostly quercetagitrin, was subjected to two different antioxidant assays (metal chelating activity and lipoxygenase inhibition) and to in vitro cytotoxicity assessment. Generally, the biological assays showed promising results for the investigated fraction compared to the initial extract. Given the encouraging outcome of the in vitro assays, further purification and structural analysis of compounds from T. erecta extracts, as well as further in vivo investigations are justified.

Chemical Profile and Antioxidant Activity of Zinnia elegans Jacq. Fractions.

Zinnia elegans (syn. Zinnia violacea) is a common ornamental plant of the Asteraceae family, widely cultivated for the impressive range of flower colors and persistent bloom. Given its uncomplicated cultivation and high adaptability to harsh landscape conditions, we investigated the potential use of Z. elegans as a source of valuable secondary metabolites. Preliminary classification of compounds found in a methanolic extract obtained from inflorescences of Z. elegans cv. Caroussel was accomplished using HR LC-MS techniques. The extract was then subjected to solid-phase extraction and separation using Sephadex LH-20 column chromatography, which resulted in several fractions further investigated for their antioxidant properties through lipoxygenase inhibition and metal chelating activity assays. Moreover, following additional purification procedures, structures of some active ingredients were established by NMR spectroscopy. The investigated fractions contained polyphenolic compounds such as chlorogenic acids and apigenin, kaempferol, and quercetin glycosides. Antioxidant assays showed that certain fractions exhibit moderate 15-LOX inhibition (Fr 2, IC50 = 18.98 µg/mL) and metal chelation (e.g., Fr 1-2, EC50 = 0.714-1.037 mg/mL) activities as compared to positive controls (20.25 µg/mL for kaempferol and 0.068 mg/mL for EDTA, respectively). For Fr 2, the 15-LOX inhibition activity seems to be related to the abundance of kaempferol glycosides. The NMR analyses revealed the presence of a kaempferol 3-O-glycoside, and a guanidine alkaloid previously not described in this species.

ANTIOXIDANT AND PHYTOBIOLOGICAL STUDIES ON TWO ALLIUM CEPA L. EXTRACTS.

AIM: To investigate the antioxidant potential of two Allium cepa L. extracts. MATERIAL AND METHODS: The antioxidant activity of the two extracts (encoded EC1 and EC2) was assessed using two methods: DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging capacity and determination of their reducing power; phytobiological, studies were performed using grains of Triticum aestivum L., Falmura variety. RESULTS AND DISCUSSIONS: EC1 and EC2 extracts had a particular affinity for binding DPPH radicals. At equivalent concentrations, the reducing power of EClextract was about 2 times higher compared to EC2, increasing with the concentration of the analyzed sample. The phytobiological study demonstrated that the investigated extracts had no cytotoxic and genotoxic potential.

Armillaria mellea: phenolic content, in vitro antioxidant and antihyperglycemic effects.

UNLABELLED: Armillaria mellea, known as honey mushroom, has been used both as food and medicine. AIM: In this work, the ethanolic and hydromethanolic extracts from Armillaria mellea fruiting bodies were investigated for their phenolic content, antioxidant and antihyperglycemic effects (in vitro studies). MATERIAL AND METHODS: The total phenolics were quantified using Folin-Ciocalteu assay. The antioxidant activity was evaluated by testing the free radical scavenging capacity, reducing power and ferrous ion chelating ability; in addition, the capacity to inhibit 15-lipoxygenase was also assessed. The antihyperglycemic activity was investigated by α-glucosidase assay. RESULTS: Total phenolic contents of 21.68 ± 0.06 and 5.70 ± 0.28 mg/g were determined in the hydromethanolic and ethanolic extracts, respectively. The hydromethanolic extract showed higher free radical scavenging and reducing abilities (EC50 = 452.6 ± 2.7, 140.57 ± 1.45 and 129.45 ± 0.98 µg/mL in DPPH, ABTS and reducing power assays, respectively). The ethanolic extract proved to be more efficient in the ferrous ion chelation, 15-lipoxygenase and α-glucosidase inhibition assays (EC55 = 67.93 ± 0.35, 290.93 ± 2.05 and 8.54 ± 0.06 µg/mL, respectively). CONCLUSIONS: Armillaria mellea extracts showed antioxidant and antihyperglycemic potential in in vitro models and therefore they are promising candidates for the development of dietary supplements and pharmaceutical products.

In vitro study on the antioxidant activity of a polyphenol-rich extract from Pinus brutia bark and its fractions.

A crude hydromethanolic extract from Pinus brutia bark and its fractions (diethyl ether, ethyl acetate, n-butanol, and aqueous fractions) were studied with regard to their phenolic content and antioxidant activities. The total phenolics and proanthocyanidins in each extract were quantified by spectrophotometric methods; the polyphenolic profile was analyzed by RP-HPLC-DAD-ESI-MS. All extracts were tested with regard to their ability to scavenge free radicals (ABTS radical cation, superoxide and hydroxyl radicals), reduce ferric ions, and inhibit 15-lipoxygenase. P. brutia bark extracts had high phenolic contents (303.79±7.34-448.90±1.39 mg/g). Except diethyl ether extract, all other extracts contained proanthocyanidins ranging from 225.79±3.94 to 250.40±1.44 mg/g. Several polyphenols were identified by RP-HPLC-DAD-ESI-MS: taxifolin in diethyl ether extract, a taxifolin-O-hexoside, catechin, procyanidin dimers, and trimers in ethyl acetate extract. Except diethyl ether extract, all other extracts were effective scavengers of superoxide and hydroxyl radicals (EC50=33.5±1.1-54.93±2.85 µg/mL and 0.47±0.06-0.6±0.0 mg/mL, respectively). All extracts had noticeable 15-lipoxygenase inhibitory effects (EC50=22.47±0.75-34.43±2.25 µg/mL). We conclude that P. brutia bark is very promising for the dietary supplements industry due to its high free radical scavenging and 15-lipoxygenase inhibitory effects.

Contributions regarding the leaf histo-anatomy of some Pelargonium species.

UNLABELLED: Pelargonium L. genra (Fam. Geraniaceae) includes over 250 species, most of it native to southern Africa. Nowadays, four perennial species are cultivated in Romania as ornamental plants. Our aim was to establish the main characteristics and differences that occur between Pelargonium zonale, P. hispidum, P. grandiflorum, P. peltatum and P. radens. All in all, the quantitative differences (regarding the leaf-stalk, blade and trichomes) have a taxonomical value for all five species included in the research. MATERIALS AND METHODS: The plant material was represented by fresh leaves harvested from 5 species of Pelargonium: P. peltatum (L.) Aiton, P. radens H.E. Moore, P. grandiflorum (Andrz.) Willd., P. hispidum. (anthesis phase) and preserved in ethanol 70%. Using a dissecting microscope, sections were made through leaf blade surface and cross sections of/ for leaf stalk and blade. RESULTS: The common characteristics of the leaf is the palmatilobate blade with long petiole. Although many studies regarded glandular trichomes from Pelargonium leaves, most of the leaf structures are still unknown. The most important for the quality and quantity of essential oil extracted from Pelargonium sp. are trichoms.

[Microbiological evaluation of some commercial brands of Chamomillae floss]. / Controlul microbiologic al unor sorturi comerciale de Chamomillae flos.

UNLABELLED: Different strains of microorganisms contaminate medicinal herb, and theirs, enzymes could modify the normal composition of herbal products. MATERIAL AND METHODS: We evaluated the microbiological quality of 12 samples of Chamomillae floss commercialized in, markets and specific stores. Microbiological quality has been evaluated according European Pharmacopoeia rules. In all samples we determined the total number of germs, total number of fungi and we evaluated the presence of coliform bacilli, respectively Staphylococcus species. RESULTS: For all samples, the total number of germs was under the limits with variation between 320 CFU/g (P 4) and 14000 CFU/g (P 11). The total number of fungi was under the limits; in most of samples, we detected Penicillium sp. and Aspergillus sp. In 4 samples the total number of Escherichia coli strains was over the limits (100 CFU/g). CONCLUSIONS: An important decrease of total number of coliform bacilli was observed after boiling water has been added but the total number of germs was decreased insignificantly, so these extracts are not very safe for patient especially when vegetal products contain a great number of germs.