Evaluating of Induction of Apoptosis by Cornus mass L. Extract in the Gastric Carcinoma Cell Line (AGS)

Aim and objectives: Natural products and derivatives of medicinal vegetation can play an important role to the cure tumor. The Present study was focused to determine the effect of Cornus mass L. extract on the induction of apoptosis in AGS gastric carcinoma cell line in compared to L929 cells. Methods: In this experimental study, AGS and L929 cells were cultured and treated with different concentrations (0­10 mg/ml) of Cornus mass L. extract for 48 and 72 hours. Cell proliferation was assessed by MTT assay. The optical density of the colored solution was quantified at 570 nm wavelengths by an ELISA Reader. Making use of the apoptosis detection kit of Annexin V-FITC, PI and double staining with Annexin V-FITC were carried out for flow cytometry investigations. Data were analyzed by ANOVA. Variations with a P-value less than 0.05 were considered significant. Results: shows a noticeable deviation among various concentrations of extract when cells were treated for 48, 72 h declined cell viability in AGS cell line in comparison L929 cell lines in a dose and time-dependent manner (P < 0.05). This extract also displayed approximately several-fold increased anti-cancer potency in AGS compared to L929 cells. The IC50 value in AGS cells (evaluated after 48,72h) of the extract against AGS cells was 5/44, 2/44 mg/ml (p≤0.05). The analysis results of flow cytometry indicated that apoptosis was induced by the extract in AGS cells treated, compared with L929 cells. Conclusion: Each of our results implicates the reality that Cornus mass L. extract acts as a novel, potent inhibitor of cancer proliferation in in vitro. This may result in developing a promising therapeutic agent for the treatment of indole-sensitive cancers.

Effect of ß-D-Mannuronic Acid (M2000) on Oxidative Stress Enzymes' Gene Using Healthy Donor Peripheral Blood Mononuclear Cells for Evaluating the Anti-Aging Property.

OBJECTIVE: This research aimed to study the anti-aging and anti-inflammatory effects of low and high doses of the ß-D-mannuronic (M2000) on gene expression of enzymes involved in oxidative stress (including SOD2, GST, GPX1, CAT, iNOS, and MPO) in peripheral blood mononuclear cells (PBMCs) of healthy donors under in vitro conditions. METHODS: The PBMCs were separated and the RNAs were then extracted and the cDNAs synthesized, and expression levels of the mentioned genes were detected by qRT-PCR. RESULTS: Our results indicated that the high dose of this drug could significantly reduce the expression level of the SOD2 gene compared to the lipopolysaccharide (LPS) group (p < 0.0001). Moreover, it was found that the high dose of this drug could significantly decrease the expression level of the GST gene compared to the LPS group (p < 0.0001). However, no significant reductions were observed in expression levels of the CAT and GPX1 genes compared to the LPS group. Furthermore, our data revealed that the level of iNOS and MPO gene expression was significantly reduced, in both doses of M2000, respectively, compared to the LPS group (p < 0.0001). CONCLUSION: This research showed that M2000 as a novel NSAID with immunosuppressive properties could modify oxidative stress through lowering expression levels of the SOD2, GST, iNOS, and MPO genes compared to the healthy expression levels, with a probable reduction of the risk of developing inflammatory diseases related to age and aging.

The regulatory impacts of Morus Alba leaf extract on some enzymes involved in glucose metabolism pathways in diabetic rat liver.

BACKGROUND: Many traditional therapies have been proposed as alternative regimens for treatment of diabetes mellitus. The Morus Alba (MA) leaf is a natural therapeutic compound which is shown to have antidiabetic properties. The aim of the present study was to determine whether MA leaf extract is capable of regulating liver enzymes that are involved in glucose metabolism pathways in normal and Streptozotocin (STZ)-induced diabetic rats. METHODS: Forty healthy adult male Wistar rats (eight weeks old) weighing about 250 +/- 10 g were taken for this experiment. The rats were divided into 4 groups with 10 rats in each group and treated through a gavage tube for a period of two months as follows: group I: non diabetic control rats with distilled water; group II: non diabetic rats with 1.0 g/kg per day; group III: diabetic control rats with distilled water and group IV: diabetic rats with MA 1.0 g/kg per day. At the end of the 8th week, serum glucose, insulin and hepatic glucokinase activity were measured using standard methods and compared between diabetic and healthy rats. We also assessed the expression of phosphofructokinase-1 enzyme at the level of mRNA, using a Real Time-PCR method. RESULTS: Findings of the present study demonstrated that MA leaf extract can significantly increase liver glucokinase activity and serum insulin levels in diabetic rats (p < 0.05). It also significantly attenuated the serum glucose level in rats compared to the control groups (p < 0.05). Also, the body weight of diabetic rats was significantly (p < 0.05) decreased as compared to their initial weight. However, the body weights of diabetic rats treated with MA increased in the same way as normal control rats. CONCLUSIONS: The present findings suggest that the antihyperglycemic action of MA is mediated by increasing liver glucokinase activity and serum insulin level. These results are additional, definite evidence supporting MA as traditional medicine for diabetic patients.