RESUMO
Long-chain n-3 PUFA (LC n-3 PUFA) prevent, in rodents, insulin resistance (IR) induced by a high-fat and/or fructose diet but not IR induced by glucocorticoids. In humans, contrasting effects have also been reported. We investigated their effects on insulin sensitivity, feed intake (FI) and body weight gain in genetically insulin resistant male obese (fa/fa) Zucker (ZO) rats during the development of obesity. ZO rats were fed a diet supplemented with 7 % fish oil (FO) + 1 % corn oil (CO) (wt/wt) (ZOFO), while the control group was fed a diet containing 8 % fat from CO (wt/wt) (ZOCO). Male lean Zucker (ZL) rats fed either FO (ZLFO) or CO (ZLCO) diet were used as controls. FO was a marine-derived TAG oil containing EPA 90 mg/g + DHA 430 mg/g. During an oral glucose tolerance test, glucose tolerance remained unaltered by FO while insulin response was reduced in ZOFO only. Liver insulin sensitivity (euglycaemic-hyperinsulinaemic clamp + 2 deoxyglucose) was improved in ZOFO rats, linked to changes in phosphoenolpyruvate carboxykinase expression, activity and glucose-6-phosphatase activity. FI in response to intra-carotid insulin/glucose infusion was decreased similarly in ZOFO and ZOCO. Hypothalamic ceramides levels were lower in ZOFO than in ZOCO. Our study demonstrates that LC n-3 PUFA can minimise weight gain, possibly by alleviating hypothalamic lipotoxicity, and liver IR in genetically obese Zucker rats.
Assuntos
Ácidos Graxos Ômega-3 , Resistência à Insulina , Humanos , Masculino , Ratos , Animais , Resistência à Insulina/fisiologia , Óleos de Peixe/farmacologia , Ratos Zucker , Glicemia/metabolismo , Insulina/metabolismo , Obesidade/metabolismo , Glucose/farmacologia , Ingestão de Alimentos , Aumento de Peso , Ácidos Graxos Insaturados/farmacologia , Óleo de Milho/farmacologia , Ácidos Graxos Ômega-3/farmacologiaRESUMO
Bile acids (BAs) improve metabolism and exert anti-obesity effects through the activation of the Takeda G protein-coupled receptor 5 (TGR5) in peripheral tissues. TGR5 is also found in the brain hypothalamus, but whether hypothalamic BA signaling is implicated in body weight control and obesity pathophysiology remains unknown. Here we show that hypothalamic BA content is reduced in diet-induced obese mice. Central administration of BAs or a specific TGR5 agonist in these animals decreases body weight and fat mass by activating the sympathetic nervous system, thereby promoting negative energy balance. Conversely, genetic downregulation of hypothalamic TGR5 expression in the mediobasal hypothalamus favors the development of obesity and worsens established obesity by blunting sympathetic activity. Lastly, hypothalamic TGR5 signaling is required for the anti-obesity action of dietary BA supplementation. Together, these findings identify hypothalamic TGR5 signaling as a key mediator of a top-down neural mechanism that counteracts diet-induced obesity.
Assuntos
Ácidos e Sais Biliares/metabolismo , Obesidade/metabolismo , Receptores Acoplados a Proteínas G/metabolismo , Animais , Peso Corporal/genética , Metabolismo Energético/genética , Células HEK293 , Humanos , Hipotálamo/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Obesos , Camundongos Transgênicos , Obesidade/genética , Obesidade/prevenção & controle , Receptores Acoplados a Proteínas G/genética , Transdução de Sinais/fisiologiaRESUMO
Since the XIX(th) century, the brain has been known for its role in regulating food intake (via the control of hunger sensation) and glucose homeostasis. Further interest has come from the discovery of gut hormones, which established a clear link between the gut and the brain in regulating glucose and energy homeostasis. The brain has two particular structures, the hypothalamus and the brainstem, which are sensitive to information coming either from peripheral organs or from the gut (via circulating hormones or nutrients) about the nutritional status of the organism. However, the efforts for a better understanding of these mechanisms have allowed to unveil a new gut-brain neural axis as a key regulator of the metabolic status of the organism. Certain nutrients control the hypothalamic homeostatic function via this axis. In this review, we describe how the gut is connected to the brain via different neural pathways, and how the interplay between these two organs drives the energy balance.
Assuntos
Encéfalo/fisiologia , Glucose/metabolismo , Homeostase/fisiologia , Intestinos/fisiologia , Animais , Regulação do Apetite/fisiologia , Vias Autônomas/fisiologia , Glicemia/metabolismo , Comportamento Alimentar/fisiologia , Hormônios Gastrointestinais/fisiologia , Gluconeogênese/fisiologia , Humanos , Fome/fisiologia , Hipotálamo/fisiologia , Intestinos/inervação , Intestinos/microbiologia , Fígado/metabolismo , Microbiota , Resposta de Saciedade/fisiologiaRESUMO
OBJECTIVE: High-protein diets favor weight loss and its maintenance. Whether these effects might be recapitulated by certain amino acids is unknown. Therefore, the impact of leucine supplementation on energy balance and associated metabolic changes in diet-induced obese (DIO) mice during and after weight loss was investigated. METHODS: DIO C57BL/6J mice were fed a normocaloric diet to induce weight loss while receiving or not the amino acid leucine in drinking water. Body weight, food intake, body composition, energy expenditure, glucose tolerance, insulin, and leptin sensitivity were evaluated. Q-PCR analysis was performed on muscle, brown and white adipose tissues. RESULTS: DIO mice decreased body weight and fat mass in response to chow, but supplementation with leucine did not affect these parameters. During weight maintenance, mice supplemented with leucine had improved glucose tolerance, increased leptin sensitivity, and lower respiratory quotient. The latter was associated with changes in the expression of several genes modulating fatty acid metabolism and mitochondrial activity in the epididymal white and the brown adipose tissues, but not muscle. CONCLUSIONS: Leucine supplementation might represent an adjuvant beneficial nutritional therapy during weight loss and maintenance, because it improves lipid and glucose metabolism and restores leptin sensitivity in previously obese animals.
Assuntos
Glicemia/metabolismo , Suplementos Nutricionais , Leucina/administração & dosagem , Tecido Adiposo Marrom/efeitos dos fármacos , Tecido Adiposo Marrom/metabolismo , Tecido Adiposo Branco/efeitos dos fármacos , Tecido Adiposo Branco/metabolismo , Animais , Composição Corporal , Calorimetria Indireta , Dieta Hiperlipídica/efeitos adversos , Metabolismo Energético/efeitos dos fármacos , Gluconeogênese/efeitos dos fármacos , Teste de Tolerância a Glucose , Insulina/sangue , Mucosa Intestinal/metabolismo , Intestinos/efeitos dos fármacos , Leptina/sangue , Metabolismo dos Lipídeos/efeitos dos fármacos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Obesos , Obesidade/tratamento farmacológico , Redução de PesoRESUMO
BACKGROUND: Leucine supplementation might have therapeutic potential in preventing diet-induced obesity and improving insulin sensitivity. However, the underlying mechanisms are at present unclear. Additionally, it is unclear whether leucine supplementation might be equally efficacious once obesity has developed. METHODOLOGY/PRINCIPAL FINDINGS: Male C57BL/6J mice were fed chow or a high-fat diet (HFD), supplemented or not with leucine for 17 weeks. Another group of HFD-fed mice (HFD-pairfat group) was food restricted in order to reach an adiposity level comparable to that of HFD-Leu mice. Finally, a third group of mice was exposed to HFD for 12 weeks before being chronically supplemented with leucine. Leucine supplementation in HFD-fed mice decreased body weight and fat mass by increasing energy expenditure, fatty acid oxidation and locomotor activity in vivo. The decreased adiposity in HFD-Leu mice was associated with increased expression of uncoupling protein 3 (UCP-3) in the brown adipose tissue, better insulin sensitivity, increased intestinal gluconeogenesis and preservation of islets of Langerhans histomorphology and function. HFD-pairfat mice had a comparable improvement in insulin sensitivity, without changes in islets physiology or intestinal gluconeogenesis. Remarkably, both HFD-Leu and HFD-pairfat mice had decreased hepatic lipid content, which likely helped improve insulin sensitivity. In contrast, when leucine was supplemented to already obese animals, no changes in body weight, body composition or glucose metabolism were observed. CONCLUSIONS/SIGNIFICANCE: These findings suggest that leucine improves insulin sensitivity in HFD-fed mice by primarily decreasing adiposity, rather than directly acting on peripheral target organs. However, beneficial effects of leucine on intestinal gluconeogenesis and islets of Langerhans's physiology might help prevent type 2 diabetes development. Differently, metabolic benefit of leucine supplementation is lacking in already obese animals, a phenomenon possibly related to the extent of the obesity before starting the supplementation.
Assuntos
Adiposidade/efeitos dos fármacos , Suplementos Nutricionais , Resistência à Insulina , Leucina/farmacologia , Animais , Dieta Hiperlipídica , Metabolismo Energético/efeitos dos fármacos , Ácidos Graxos/metabolismo , Glucose/metabolismo , Homeostase/efeitos dos fármacos , Insulina/farmacologia , Leucina/sangue , Lipídeos/sangue , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Obesos , Oxirredução/efeitos dos fármacos , Fenótipo , Aumento de Peso/efeitos dos fármacosRESUMO
The detection of glucose in the hepatoportal area is a simple but crucial peripheral cue initiating a nervous signal that ultimately leads to a wide array of metabolic and behavioural responses, such as decreased food intake, tighter control of glucose homeostasis, or appearance of food preference. This signal has been suggested to mediate the effects of high-protein diets, as opposed to high-fat/high-sucrose diets. Nevertheless, the central targets of the signal originating from the hepatoportal area remain largely undocumented. Using immunohistochemistry on the brain of male rats, we show here that portal glucose increases c-Fos expression in the brainstem, in the hypothalamus (in particular in neurons expressing pro-opiomelanocortin) and also in olfactory and other limbic and cortical areas, including those functionally implicated in reward (Experiment 1). In similar postabsorptive conditions, a high-protein diet induced similar effects in the hypothalamus and the granular cells of the main olfactory bulb, whereas the high-fat/high-sucrose diet actually reduced the basal expression of c-Fos in cortical layers. Both diets also decreased the number of neurons expressing c-Fos in the amygdala and gustatory areas (Experiment 2). Altogether, these findings suggest that the peripheral signal primed by portal glucose sensing may influence behavioural adaptation such as food preference via a network including the olfactory pathway, central amygdala, nucleus accumbens and orbitofrontal cortex, in addition to satiety and metabolic effects primarily implicating the hypothalamic response.
Assuntos
Córtex Cerebral/metabolismo , Glucose/fisiologia , Hipotálamo/metabolismo , Bulbo Olfatório/metabolismo , Sistema Porta/fisiologia , Recompensa , Animais , Tronco Encefálico/metabolismo , Tronco Encefálico/fisiologia , Córtex Cerebral/fisiologia , Ingestão de Alimentos/fisiologia , Hipotálamo/fisiologia , Masculino , Bulbo Olfatório/fisiologia , Ratos , Ratos Sprague-DawleyRESUMO
The hypothalamic melanocortin system--the melanocortin receptor of type 4 (MC4R) and its ligands: α-melanin-stimulating hormone (α-MSH, agonist, inducing hypophagia), and agouti-related protein (AgRP, antagonist, inducing hyperphagia)--is considered to play a central role in the control of food intake. We tested its implication in the mediation of the hunger-curbing effects of protein-enriched diets (PED) in mice. Whereas there was a 20% decrease in food intake in mice fed on the PED, compared to mice fed on an isocaloric starch-enriched diet, there was a paradoxical decrease in expression of the hypothalamic proopiomelanocortin gene, precursor of α-MSH, and increase in expression of the gene encoding AgRP. The hypophagia effect of PED took place in mice with invalidation of either MC4R or POMC, and was even strengthened in mice with ablation of the AgRP-expressing neurons. These data strongly suggest that the hypothalamic melanocortin system does not mediate the hunger-curbing effects induced by changes in the macronutrient composition of food. Rather, the role of this system might be to defend the body against the variations in food intake generated by the nutritional environment.
Assuntos
Proteínas Alimentares/farmacologia , Ingestão de Alimentos/efeitos dos fármacos , Hipotálamo/efeitos dos fármacos , Hipotálamo/metabolismo , Proteína Relacionada com Agouti/genética , Proteína Relacionada com Agouti/metabolismo , Animais , Peso Corporal/efeitos dos fármacos , Ingestão de Alimentos/genética , Masculino , Camundongos , Pró-Opiomelanocortina/genética , Pró-Opiomelanocortina/metabolismo , Receptor Tipo 4 de Melanocortina/genética , Receptor Tipo 4 de Melanocortina/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , alfa-MSH/genética , alfa-MSH/metabolismoRESUMO
To study the consequences of maternal obesity during gestation and suckling periods on metabolic features and expression of genes belonging to the melanocortinergic system, we developed Diet-Induced-Obesity (DIO) in mice fed high-fat-diet (HFD). After weaning, F1-descendants were fed the same diet than dams up to 16 weeks or received a 2-week standard chow at several time points. From birth, F1-DIO displayed higher body weight than F1-control. Hyperinsulinemia, hypertriglyceridemia, hyperleptinemia were detected from P10 and fasting hyperglycaemia from 2 week-post-weaning. From late gestation to 16-week-post-weaning the expression of MC4-R gene and/or the POMC/AgRP ratio was increased, suggesting an activation of this pathway to compensate the deleterious effects of HFD. Standard chow replacement at weaning normalized metabolic status but a partial recovery was obtained for later changes. Concomitant variations in the expression of the melanocortinergic genes were observed. Therefore, early nutritional intervention could override the impact of maternal and postnatal over-nutrition.
Assuntos
Proteína Relacionada com Agouti/genética , Hipotálamo/metabolismo , Obesidade/metabolismo , Efeitos Tardios da Exposição Pré-Natal/metabolismo , Pró-Opiomelanocortina/genética , Fatores Etários , Proteína Relacionada com Agouti/metabolismo , Análise de Variância , Fenômenos Fisiológicos da Nutrição Animal , Animais , Dieta , Gorduras na Dieta , Feminino , Técnicas Imunoenzimáticas , Insulina/sangue , Leptina/sangue , Masculino , Camundongos , Estado Nutricional/genética , Obesidade/genética , Gravidez , Efeitos Tardios da Exposição Pré-Natal/genética , Pró-Opiomelanocortina/metabolismo , Receptor Tipo 4 de Melanocortina/genética , Receptor Tipo 4 de Melanocortina/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Triglicerídeos/sangueRESUMO
BACKGROUND: Deregulation of hypothalamic fatty acid sensing lead to hepatic insulin-resistance which may partly contribute to further impairment of glucose homeostasis. METHODOLOGY: We investigated here whether hypothalamic nitric oxide (NO) could mediate deleterious peripheral effect of central lipid overload. Thus we infused rats for 24 hours into carotid artery towards brain, either with heparinized triglyceride emulsion (Intralipid, IL) or heparinized saline (control rats). PRINCIPAL FINDINGS: Lipids infusion led to hepatic insulin-resistance partly related to a decreased parasympathetic activity in the liver assessed by an increased acetylcholinesterase activity. Hypothalamic nitric oxide synthases (NOS) activities were significantly increased in IL rats, as the catalytically active neuronal NOS (nNOS) dimers compared to controls. This was related to a decrease in expression of protein inhibitor of nNOS (PIN). Effect of IL infusion on deregulated hepatic insulin-sensitivity was reversed by carotid injection of non selective NOS inhibitor NG-monomethyl-L-arginine (L-NMMA) and also by a selective inhibitor of the nNOS isoform, 7-Nitro-Indazole (7-Ni). In addition, NO donor injection (L-arginine and SNP) within carotid in control rats mimicked lipid effects onto impaired hepatic insulin sensitivity. In parallel we showed that cultured VMH neurons produce NO in response to fatty acid (oleic acid). CONCLUSIONS/SIGNIFICANCE: We conclude that cerebral fatty acid overload induces an enhancement of nNOS activity within hypothalamus which is, at least in part, responsible fatty acid increased hepatic glucose production.
Assuntos
Resistência à Insulina , Fígado/fisiologia , Óxido Nítrico/fisiologia , Ácido Oleico/administração & dosagem , Animais , Inibidores Enzimáticos/farmacologia , Comportamento Alimentar , Glucose/metabolismo , Hipotálamo/enzimologia , Óxido Nítrico/biossíntese , Doadores de Óxido Nítrico/farmacologia , Óxido Nítrico Sintase Tipo I/antagonistas & inibidores , RatosRESUMO
The objectives of this study were to identify potential alterations in gene expression of melanocortin-4 receptor (MC4-R), proopiomelanocortin (POMC), and Agouti-related protein (AgRP) in mouse hypothalamus under a chronic peripheral infusion of leptin or at early (8 weeks) and advanced (16 weeks) phases of diet-induced obesity. Control or diet-induced obesity mice (8 or 16 weeks of high-fat diet) were either treated or not treated with leptin. Metabolic features were analyzed and expression of the genes of interest was measured by quantitative reverse transcriptase-PCR (RT-qPCR) and western blot. We reported that in control mice, but not in obese mice, leptin infusion induced an increase in POMC mRNA level as well as in MC4-R mRNA level suggesting that leptin could act directly and/or through alpha-melanocyte-stimulating hormone (alpha-MSH). This hypothesis was reinforced after in vitro studies, using the mouse hypothalamic GT1-7 cell line, since both leptin and Norleucine(4), D-Phenylalanine(7)-alpha-MSH (NDP-alpha-MSH) treatments increased MC4-R expression. After 8 weeks of high-fat diet, nondiabetic obese mice became resistant to the central action of leptin and their hypothalamic content of POMC and AgRP mRNA were decreased without modification of MC4-R mRNA level. After 16 weeks of high-fat diet, mice exhibited more severe metabolic disorders with type 2 diabetes. Moreover, hypothalamic expression of MC4-R was highly increased. In conclusion, several alterations of the melanocortin system were found in obese mice that are probably consecutive to their central resistance to leptin. Moreover, when the metabolic status is highly degraded (with all characteristics of a type 2 diabetes), other regulatory mechanisms (independent of leptin) can also take place.
Assuntos
Hipotálamo/efeitos dos fármacos , Hipotálamo/metabolismo , Leptina/fisiologia , Melanocortinas/metabolismo , Obesidade/metabolismo , Proteína Relacionada com Agouti/metabolismo , Animais , Linhagem Celular , Diabetes Mellitus Tipo 2/metabolismo , Modelos Animais de Doenças , Hipotálamo/citologia , Infusões Parenterais , Leptina/administração & dosagem , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Norleucina/farmacologia , Pró-Opiomelanocortina/metabolismo , RNA Mensageiro/metabolismo , Receptor Tipo 4 de Melanocortina/metabolismo , alfa-MSH/análogos & derivados , alfa-MSH/farmacologiaRESUMO
The interactions between glucose and energy homeostasis are well known. In particular, a high portal vein glucose concentration suppresses food intake. Numerous studies point to a glucose-sensing mechanism involving nerves present in the wall of the portal vein. We have studied the expression of genes involved in gluconeogenesis in the rat and human intestine, in terms of mRNA and protein levels and enzyme activity. Intestinal glucose production was quantified by using a combination of (3-3H) glucose tracer dilution and arterio-venous glucose balance. The effect of the portal glucose level on food intake was studied in conscious rats with indwelling portal vein catheters. The hypothalamic consequences of glucose infusion were studied by c-Fos protein immunodetection. All regulatory genes involved in gluconeogenesis were strongly induced by fasting and by a protein-rich diet. In both cases the glucose level in the portal vein increased, an effect that lasted some time after a protein-rich meal. Glucose infusion into the portal vein led to a decrease in food intake and activated hypothalamic regions involved in controlling food intake, in the same way as the protein-rich diet. The effects of portal glucose infusion and of the protein-rich diet were suppressed by inactivating the portal nervous system. These results provide a mechanistic explanation for the effect of satiety induced by a high-protein diet.
Assuntos
Glicemia/metabolismo , Proteínas Alimentares , Comportamento Alimentar/fisiologia , Gluconeogênese , Glucose/metabolismo , Hipotálamo/fisiologia , Intestino Delgado/metabolismo , Animais , Regulação do Apetite/fisiologia , Diabetes Mellitus Experimental/metabolismo , Ingestão de Alimentos/fisiologia , Metabolismo Energético , Gluconeogênese/genética , Glucose/administração & dosagem , Homeostase , Humanos , Hipotálamo/metabolismo , Veia Porta/metabolismo , Ratos , Resposta de SaciedadeRESUMO
Protein feeding is known to decrease hunger and subsequent food intake in animals and humans. It has also been suggested that glucose appearance into portal vein, as occurring during meal assimilation, may induce comparable effects. Here, we connect these previous observations by reporting that intestinal gluconeogenesis (i.e., de novo synthesis of glucose) is induced during the postabsorptive time (following food digestion) in rats specifically fed on protein-enriched diet. This results in glucose release into portal blood, counterbalancing the lowering of glycemia resulting from intestinal glucose utilization. Comparable infusions into the portal vein of control postabsorptive rats (fed on starch-enriched diet) decrease food consumption and activate the hypothalamic nuclei regulating food intake. Similar hypothalamic activation occurs on protein feeding. All these effects are absent after denervation of the portal vein. Thus, portal sensing of intestinal gluconeogenesis may be a novel mechanism connecting the macronutrient composition of diet to food intake.