Role of Dau c 1 in three different patterns of carrot-induced asthma.

OBJECTIVE: To assess the role of Dau c 1 in three patients with carrot induced asthma. MATERIAL AND METHODS: Patient 1 had asthma when handling raw carrots. Sensitization to pollens wasn't detected. Patient 2 had rhinoconjunctivitis due to grass and olive pollen allergy. She had asthma when handling raw carrots. Patient 3 was diagnosed of rhinoconjunctivitis and asthma due to allergic sensitization to mites, several pollens and cat. She had asthma due to raw carrot ingestion and inhalation. IgE immunobot analysis and ELISA inhibition assay were used to investigate the allergens and specific antibodies. RESULTS: IgE Immunoblot Analysis: Dau c 1 from carrot extract and the recombinant rDau c 1 were recognized by IgE from patients 1 and 2. Band of Bet v 1 in birch pollen extract wasn't recognized. Patient 3 didn't recognize any of these allergens. Specific IgE to rDau c 1 was measured by ELISA. Specific IgE ELISA-inhibition with carrot as solid phase showed an intermediate inhibition (30 %) between carrot and rDau c 1 in patient 1; and a considerable inhibition (nearly 100 %) between carrot and rDau c 1 in patient 2. No inhibition was found in patient 3. Specific IgE ELISA inhibition between rDau c 1 and rBet v 1, employing rDau c 1 as solid phase was made in patients 1 and 2. Bet v 1 showed less than 40 % of inhibition of rDau c 1 in patient 1; and an intermediate inhibition (> 40 %) between rBet v 1 and rDau c 1 in patient 2. CONCLUSIONS: Airborne carrot allergens are able to sensitize without the implication of a previous pollen allergy. Dau c 1 was the main allergen in patient 2. In patient 1, there was a band of 30 kd that looks like the predominant allergen. Patients 1 and 2 were sensitized directly from carrot allergens. In patient 3, Dau c 1 isn't related to the carrot allergy. Allergy to carrot in patient 3 seems to be related to her allergy to different pollens; however, it wasn't related to birch pollen. Mediterranean countries didn't show the same patterns of food-related pollen allergy than Nordic countries.

Role of Dau c 1 in three different patterns of carrot-induced asthma / Papel de Dau c 1 en tres modelos de asma inducida por zanahoria

Objective: To assess the role of Dau c 1 in three patients with carrot induced asthma. Material and methods: Patient 1 had asthma when handling raw carrots. Sensitization to pollens wasn't detected. Patient 2 had rhinoconjunctivitis due to grass and olive pollen allergy. She had asthma when handling raw carrots. Patient 3 was diagnosed of rhinoconjunctivitis and asthma due to allergic sensitization to mites, several pollens and cat. She had asthma due to raw carrot ingestion and inhalation. IgE immunobot analysis and ELISA inhibition assay were used to investigate the allergens and specific antibodies. Results: IgE Immunoblot Analysis: Dau c 1 from carrot extract and the recombinant rDau c 1 were recognized by IgE from patients 1 and 2. Band of Bet v 1 in birch pollen extract wasn't recognized. Patient 3 didn't recognize any of these allergens. Specific IgE to rDau c 1 was measured by ELISA. Specific IgE ELISA-inhibition with carrot as solid phase showed an intermediate inhibition (30 %) between carrot and rDau c 1 in patient 1; and a considerable inhibition (nearly 100 %) between carrot and rDau c 1 in patient 2. No inhibition was found in patient 3. Specific IgE ELISA inhibition between rDau c 1 and rBet v 1, employing rDau c 1 as solid phase was made in patients 1 and 2. Bet v 1 showed less than 40 % of inhibition of rDau c 1 in patient 1; and an intermediate inhibition (> 40 %) between rBet v 1 and rDau c 1 in patient 2. Conclusions: Airborne carrot allergens are able to sensitize without the implication of a previous pollen allergy. Dau c 1 was the main allergen in patient 2. In patient 1, there was a band of 30 kd that looks like the predominant allergen. Patients 1 and 2 were sensitized directly from carrot allergens. In patient 3, Dau c 1 isn't related to the carrot allergy. Allergy to carrot in patient 3 seems to be related to her allergy to different pollens; however, it wasn't related to birch pollen. Mediterranean countries didn't show the same patterns of food-related pollen allergy than Nordic countries

Objetivo: Valorar la responsabilidad del Dau c 1 en tres pacientes con asma causada por zanahoria. Material y métodos: El paciente 1 tenía asma cuando manejaba zanahorias crudas, en el que no se detectó sensibilización a pólenes. La paciente 2 tenía rinoconjuntivitis causada por polen de gramíneas y de olivo, y además presentaba asma cuando manejaba zanahorias crudas. La paciente 3 padecía rinoconjuntivitis y asma, estando sensibilizada a ácaros, varios pólenes y epitelio de gato, además tenía crisis de asma tras ingestión de zanahoria y también por inhalación al manejarla. La investigación de alergenos y anticuerpos específicos (IgE) se llevó a cabo con técnicas de immunoblot y ELISA-inhibición Resultados: Análisis de IgE por immunoblot: Dau c 1 de extracto de zanahoria y el recombinante rDau c 1 fueron reconocidos por la IgE de los pacientes 1 y 2, mientras que no se reconoció la banda de Bet v 1 del extracto de polen de abedul. En la paciente 3 no se reconoció ninguno de estos alergenos. La IgE específica para rDau c 1 se midió por ELISA-inhibición con zanahoria como fase sólida, mostrando una inhibición intermedia (30%) entre zanahoria y rDau c 1 en en el paciente 1 y una inhibición considerable (próxima al 100%) entre zanahoria y rDacu c 1 en la paciente 2. En la paciente 3 no se encontró ninguna inhibición. ELISA-inhibición IgE específica entre rDau c 1 y rBet v 1, empleando rDau c 1 como fase sólida, se realizó en los pacientes 1 y 2. Bet v 1 mostró menos del 40% de inhibición de rDau c 1 en el paciente 1, y una inhibición intermedia (>40%) entre rBet v 1 y rDau c 1 en la paciente 2. Conclusiones: Los alergenos de zanahoria son capaces de sensibilizar por inhalación, sin implicación previa del polen como alergeno. Dau c 1 fue el principal alérgeno en la paciente 2. En el paciente 1 hubo una banda de 30kd que parece ser el alergeno predominante. Los pacientes 1 y 2 se sensibilizaron directamente de los alergenos de la zanahoria. En la paciente 3 el Dau c 1 no parece estar relacionado con su proceso; en esta paciente la alergia a la zanahoria parece más relacionada con la alergia a diferen tes pólenes, aunque no parece que sea el polen de abedul. En los países mediterráneos no se encuentran los mismos patrones de alergia polen-alimentos que en los países nórdicos