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1.
Br J Dermatol ; 160(6): 1172-9, 2009 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-19416272

RESUMO

BACKGROUND: Atopic dermatitis (AD) is a chronic disease with a Th2-type-cytokine dominant profile. Several cytokines and related peptides have been used for the treatment of AD but they were ineffective because of their limited biological half-life. We have recently developed a highly efficient mouse dominant negative interleukin (IL)-4/IL-13 antagonist (IL-4DM), which blocks both IL-4 and IL-13 signal transductions. OBJECTIVE: To examine the effects of IL-4DM in vivo in an AD model induced by the repeated exhibition of oxazolone (OX). METHODS: Plasmid DNA was injected intraperitoneally to cause an experimental AD-like dermatitis. The effect was evaluated by ear thickness, histological findings, and mast cells counts in the inflamed skin. The plasma IgE and histamine levels were measured. Cytokine production in skin and splenocytes were also analysed. RESULTS: Mice treated with control plasmid developed marked dermatitis with mast cells and eosinophil infiltration, and had increased plasma IgE and histamine levels with a Th2 type splenocyte cytokine profile. Treatment with mouse IL-4 DNA augmented the ear swelling and thickness with an increased dermal eosinophil count, plasma histamine level, and production of splenocyte IL-4. However, IL-4DM treatment successfully controlled the dermatitis, decreased the mast cell and eosinophil count, and suppressed plasma IgE and histamine levels. Splenocytes produced an increased level of IFN-gamma. CONCLUSION: These data showed that the simultaneous suppression of IL-4/IL-13 signals successfully controlled Th2-type chronic dermatitis. IL-4DM DNA treatment is a potent therapy for AD and related diseases.


Assuntos
Adjuvantes Imunológicos/uso terapêutico , Dermatite Atópica/tratamento farmacológico , Interleucina-13/antagonistas & inibidores , Interleucina-4/antagonistas & inibidores , Células Th2/imunologia , Vacinas de DNA/uso terapêutico , Animais , Dermatite Atópica/imunologia , Modelos Animais de Doenças , Avaliação Pré-Clínica de Medicamentos , Interleucina-13/imunologia , Interleucina-4/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Estatística como Assunto
2.
Int J Hyperthermia ; 22(1): 1-14, 2006 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-16423749

RESUMO

PURPOSE: The purpose of this study was to assess the pain relief in patients with unresectable and recurrent colorectal cancer treated with radiation plus 8 MHz radiofrequency-capacitive regional hyperthermia and to identify predictors of the good outcome. METHODS: Between February 1986-May 2003, 41 patients with primarily unresectable and recurrent colorectal cancer that caused pain were treated with thermoradiotherapy at the hospital and retrospectively analysed. Radiotherapy was administered with a mean total radiation dose of 56 Gy. Hyperthermia was usually applied within 30 min after radiotherapy once or twice a week. For cooling of the skin surface, the overlay boluses were applied in addition to regular boluses. The external cooling unit has been used to reinforce the cooling ability of the overlay bolus and achieve strong surface cooling to reduce the preferential heating of the subcutaneous fat tissue and treat with more RF-output in 17 patients since January 1997. RESULTS: Pain relief was obtained in 83% of the patients. Multi-variate analysis by logistic regression to evaluate the effects of certain factors on pain relief (complete response + good response) was strongly correlated with the presence of radiating pain to leg(s) (p < 0.05). The median follow-up was 18 months. The median duration of pain relief was 7.0 months. For the 27 patients in whom the tumour temperature was estimated, the median duration of pain relief was 14.6 months for the patients with a mean average tumour temperature of > 42.5 degrees C and 5.7 months for those of < 42.5 degrees C (p < 0.05). In the 18 patients with radiating pain to leg(s), use of strong superficial cooling and the higher numbers of hyperthermia treatments were better prognostic factors for the duration of pain relief (p < 0.01 and p < 0.05, respectively). CONCLUSIONS: Radiotherapy with 8 MHz radiofrequency-capacitive regional hyperthermia provided an efficient, effective means on pain relief of treating unresectable and recurrent colorectal cancer. The duration of pain relief can be prolonged, if an adequate heating is achieved, especially in the patients with radiating pain to the leg(s).


Assuntos
Neoplasias Colorretais/complicações , Hipertermia Induzida , Dor/radioterapia , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/radioterapia , Terapia Combinada , Feminino , Humanos , Masculino , Análise Multivariada , Radioterapia/métodos , Recidiva , Estudos Retrospectivos
3.
Eur J Cancer Prev ; 14(2): 101-5, 2005 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-15785313

RESUMO

The modifying effects of dietary administration of an herb, Terminalia catappa (TC), were investigated on rat colon carcinogenesis induced by a carcinogen azoxymethane (AOM). The number of aberrant crypt foci (ACF) and beta-catenin accumulated crypts (BCACs) in the colon, and proliferating cell nuclear antigen (PCNA) labelling index in the colonic epithelium were examined in a total of 36 male F344 rats. All animals were randomly divided into five experimental groups (4-10 rats in each group). At 6 weeks of age, rats in groups 1, 2 and 3 were given s.c. injections of AOM once a week for 2 weeks at a concentration of 20 mg/kg body weight. One week before the first injection of AOM, rats in groups 2 and 3 were fed a diet containing 0.02 and 0.1% TC, respectively, throughout the experiment. Rats in group 4 were fed a diet containing 0.1% TC. Rats in group 5 were served as untreated controls. All animals were sacrificed at the experimental week 5 after the start of the experiment. Oral administration of TC at both doses significantly decreased the numbers of both ACF/colon/rat (P<0.05 for 0.02% TC, P<0.005 for 0.1% TC) and BCAC/cm/rat (P<0.05 for both 0.02 and 0.1% TC), when compared with the control group (group 1). Colonic PCNA labelling index in groups 2 and 3 was also significantly lower than that in group 1 (P<0.001 for 0.02% TC, P<0.005 for 0.1% TC). These results suggest that TC has a potent short-term chemopreventive effect on biomarkers of colon carcinogenesis and this effect may be associated with the inhibition of the development of ACF and BCACs.


Assuntos
Biomarcadores Tumorais/sangue , Neoplasias do Colo/prevenção & controle , Extratos Vegetais/farmacologia , Terminalia/química , Administração Oral , Animais , Azoximetano/administração & dosagem , Azoximetano/toxicidade , Carcinógenos/administração & dosagem , Carcinógenos/toxicidade , Transformação Celular Neoplásica , Quimioprevenção , Doenças do Colo/induzido quimicamente , Doenças do Colo/prevenção & controle , Doenças do Colo/veterinária , Neoplasias do Colo/induzido quimicamente , Neoplasias do Colo/veterinária , Masculino , Fitoterapia/veterinária , Extratos Vegetais/administração & dosagem , Antígeno Nuclear de Célula em Proliferação/sangue , Distribuição Aleatória , Ratos , Ratos Endogâmicos F344
4.
J Clin Neurosci ; 8(1): 46-8, 2001 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11148078

RESUMO

A hypothalamic hamartoma associated with an arachnoid cyst in an 8-year-old boy is reported herein. He presented with precocious puberty, and neuroimaging studies demonstrated a solid mass in the prepontine cistern and a huge arachnoid cyst in the left cranial fossa. The mass appeared isointense to the surrounding cerebral cortex on T1-weighted magnetic resonance images, hyperintense on T2-weighted images, and was not enhanced after administration of Gd-DTPA. The patient underwent a left frontotemporal craniotomy and a cyst-peritoneal shunt was inserted. Histological features of the cyst wall and the mass were characteristic of an arachnoid cyst and hamartoma, respectively. While a hypothalamic hamartoma associated with an arachnoid cyst is rare, such a case may help clarify the geneses of both anomalous lesions.


Assuntos
Cistos Aracnóideos/complicações , Aracnoide-Máter/patologia , Hamartoma/complicações , Doenças Hipotalâmicas/complicações , Hipotálamo/patologia , Aracnoide-Máter/diagnóstico por imagem , Aracnoide-Máter/cirurgia , Cistos Aracnóideos/patologia , Cistos Aracnóideos/cirurgia , Derivações do Líquido Cefalorraquidiano/métodos , Criança , Hamartoma/patologia , Hamartoma/cirurgia , Humanos , Doenças Hipotalâmicas/patologia , Doenças Hipotalâmicas/cirurgia , Hipotálamo/diagnóstico por imagem , Hipotálamo/cirurgia , Imageamento por Ressonância Magnética , Masculino , Puberdade Precoce/etiologia , Puberdade Precoce/patologia , Puberdade Precoce/cirurgia , Tomografia Computadorizada por Raios X , Resultado do Tratamento
5.
Cancer Lett ; 148(1): 19-25, 2000 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-10680588

RESUMO

Our previous study demonstrated that the herbal medicine, Oren-to, had antitumor effects on esophageal cancer cells (ECCs) in vitro. The purpose of this study was to examine which of the seven constituents of Oren-to had antitumor effects on esophageal cancer cells. MTT assay showed that, of the seven constituents, only the aqueous extract of Coptidis Rhizoma had potent inhibitory effect on the proliferation of two types of ECC lines, YES-3 and YES-4. In addition, the proliferation of all six types of ECC lines (YES-1 to YES-6) was inhibited in a dose-dependent manner (P<0.001 for all), when co-cultured at each concentration of Coptidis Rhizoma for 72 h. The ID50 of Coptidis Rhizoma for YES-1 to YES-6 was 2.2 microg/ml, 3.0 microg/ml, 0.25 microg/ml, 2.8 microg/ml, 2.5 microg/ml, and 0.5 microg/ml, respectively, berberine, one of protoberberine components of Coptidis Rhizoma, showed potent antitumor effects on all six types of ECC lines as well as Coptidis Rhizoma. In addition, the ID50 of berberine showed a positive correlation with that of Coptidis Rhizoma in six types of ECC lines examined (r2 = 0.763, P = 0.023). Cell cycle analysis of Coptidis Rhizoma-treated cancer cells showed the accumulation of cells in the G0/G1 phase and relative decrease of the S phase. These results support the possibility that the use of Coptidis Rhizoma containing abundant berberine may be useful as one of alternative therapies for esophageal cancers.


Assuntos
Antineoplásicos/farmacologia , Berberina/farmacologia , Medicamentos de Ervas Chinesas/farmacologia , Neoplasias Esofágicas/patologia , Antineoplásicos/química , Antineoplásicos/uso terapêutico , Berberina/análise , Berberina/uso terapêutico , Ciclo Celular/efeitos dos fármacos , Divisão Celular/efeitos dos fármacos , Coptis chinensis , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/uso terapêutico , Neoplasias Esofágicas/tratamento farmacológico , Citometria de Fluxo , Formazans , Humanos , Sais de Tetrazólio , Células Tumorais Cultivadas
6.
Gynecol Oncol ; 76(2): 170-5, 2000 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-10637066

RESUMO

OBJECTIVE: A lipoprotein fraction extracted from rice bran (RBF) has been proved to be nontoxic to normal cells and to possess the ability to derange energy metabolism and induce apoptosis in Ehrlich ascites tumor cells. This study is designed to investigate the therapeutic effects of RBF as an antitumor substance on endometrial adenocarcinoma cells (Sawano). METHODS: Cultured human endometrial adenocarcinoma cells were treated with medium only (untreated), DMSO, or RBF at 100, 200, and 300 microg/ml. Mitotic indexes were counted by Giemsa stain and apoptotic index by fluorescent TUNEL stain for confocal laser scanning microscopy. DNA fragments were examined by agarose gel electrophoresis. RESULTS: Characteristic apoptotic morphology was observed in Giemsa-stained cells and further confirmed by confocal laser scanning microscopy in RBF-treated cells. Growth of Sawano cells was inhibited by RBF in a dose- and time-dependent manner. Apoptotic indexes (AI) showed an increasing tendency, and mitotic indexes (MI) showed a downward tendency. In 300 microg/ml RBF-treated Sawano cells, AI even reached 41.62 +/- 2. 23% and MI decreased to 0.79 +/- 0.08% at 96 h. Both showed significant differences from to controls and other RBF-treated groups. DNA agarose electrophoresis revealed DNA fragmentation in a ladder pattern. CONCLUSIONS: RBF can induce apoptosis of cultured human endometrial adenocarcinoma cells (Sawano) and inhibit its cell proliferation as an antitumor substance.


Assuntos
Adenocarcinoma/tratamento farmacológico , Antineoplásicos Fitogênicos/uso terapêutico , Apoptose , Neoplasias do Endométrio/tratamento farmacológico , Lipoproteínas/uso terapêutico , Oryza , Fitoterapia , Proteínas de Plantas/uso terapêutico , Adenocarcinoma/patologia , Divisão Celular/efeitos dos fármacos , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Neoplasias do Endométrio/patologia , Feminino , Humanos , Fatores de Tempo , Células Tumorais Cultivadas/efeitos dos fármacos
7.
Gut ; 41(4): 487-93, 1997 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-9391247

RESUMO

BACKGROUND: It is well established that glutamine supplemented elemental diets result in less severe intestinal damage in experimental colitis. However, few studies have examined the mode of action of glutamine in reducing intestinal damage. AIMS: To examine the effects of glutamine supplemented elemental diets on the potent inflammatory cytokines interleukin 8 (IL-8) and tumour necrosis factor alpha (TNF-alpha) in trinitrobenzene sulphonic acid (TNBS) induced colitis which presents with both acute and chronic features of ulcerative colitis. METHODS: Sprague-Dawley rats were randomised into three dietary groups and fed 20% casein (controls), or 20% casein supplemented with either 2% glutamine (2% Gln) or 4% glutamine (4% Gln). After two weeks they received intracolonic TNBS to induce colitis. RESULTS: Both Gln groups of rats gained more weight than the control group (p < 0.05) which had progressive weight loss. Colon weight, macroscopic, and microscopic damage scores for the Gln groups were lower than in the control group (p < 0.05). IL-8 and TNF-alpha concentrations in inflamed colonic tissues were lower in the Gln groups than in the control group (p < 0.05), and correlated well with disease severity. Bacterial translocation was lower both in incidence (p < 0.05) and in the number of colony forming units (p < 0.05) for the Gln groups, than in the control group. With respect to all indices studied, the 4% Gln group performed better than did the 2% Gln group. CONCLUSION: Prophylactic glutamine supplementation modulates the inflammatory activities of IL-8 and TNF-alpha in TNBS induced colitis.


Assuntos
Colite/prevenção & controle , Glutamina/administração & dosagem , Interleucina-8/biossíntese , Fator de Necrose Tumoral alfa/biossíntese , Análise de Variância , Animais , Peso Corporal , Colite/induzido quimicamente , Colite/imunologia , Colite/patologia , Colo/imunologia , Colo/microbiologia , Colo/patologia , Dieta , Modelos Animais de Doenças , Esquema de Medicação , Feminino , Mucosa Intestinal/patologia , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Estatísticas não Paramétricas , Ácido Trinitrobenzenossulfônico
8.
Pathol Int ; 47(7): 430-5, 1997 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-9234380

RESUMO

The effects of traditional Chinese medicine (Sairei-to) on experimental glomerulonephritis induced in rats by monoclonal antibody (mAb) 1-22-3 injection was examined. The level of proteinuria in the Sairei-to-treated group was significantly lower than that in the PBS treated group. This suppressive effect was caused by the major component of Sairei-to, Syo-saiko-to but not by another component, Gorel-san. The suppressive effect of Syo-saiko-to was identified in its components (Bupleuri radix, Pinelliae tuber and Zingiberis rhizoma), but not in the other combined components (Ginseng radix and Zizyphi fructus). Further study revealed that the suppressive effects of the combined components were mainly derived from Bupleuri radix. It was demonstrated that the actual active ingredient is probably Saikosaponin-d. Light microscopy revealed that Sairei-to and its effective components suppressed the proliferation of mesangial cells and mesangial matrix expansion. Semiquantitative morphological studies of glomerular lesions on the eighth day showed that Syo-saiko-to and its combined components (Bupleuri radix, Zingiberis rhizoma and Pinelliae tuber) suppressed mesangial matrix expansion significantly compared with phosphate-buffered saline control groups (matrix score: 28.0 +/- 19.1 vs 102.3 +/- 14.1; 30.9 +/- 30.1 vs 102.3 +/- 14.1, P < 0.005, respectively). It was concluded that Saikosaponin-d, as well as Bupleuri radix, Syo-saiko-to and Sairei-to can suppress proteinuria and morphological changes in the rat glomerulonephritis model induced by mAb 1-22-3.


Assuntos
Medicamentos de Ervas Chinesas/farmacologia , Glomerulonefrite/prevenção & controle , Rim/efeitos dos fármacos , Ácido Oleanólico/análogos & derivados , Proteinúria/prevenção & controle , Saponinas , Animais , Anti-Inflamatórios não Esteroides/administração & dosagem , Anticorpos Monoclonais , Análise Química do Sangue , Feminino , Glomerulonefrite/etiologia , Rim/patologia , Ratos , Ratos Wistar , Sapogeninas/administração & dosagem
9.
Acta Neurochir (Wien) ; 139(4): 336-42, 1997.
Artigo em Inglês | MEDLINE | ID: mdl-9202774

RESUMO

Twenty patients (M 11, F9; ranging from 1-77 years old) with histologically proven glial tumours in the thalamic region, treated from 1979 until 1994 at Kyushu University Hospital were retrospectively reviewed and analysed in order to elucidate their clinical and neuropathological characteristics. The initial common clinical manifestations were those of increased intracranial pressure or motor weakness. The histological diagnosis of the tumour was pilocytic astrocytoma in 2 patients, fibrillary astrocytoma in 7, anaplastic astrocytoma in 7, and glioblastoma multiforme in 4. The initial treatment was surgery alone in 4 patients, surgery followed by radiation therapy in 5, surgery followed by radiation therapy and chemotherapy in 9, and conventional radiation therapy alone in 2 patients. The 3-year overall actuarial survival rate for all patients was 20% but was related to both the histological type and the age of the patients: As a result, the rate was 44% for patients with low-grade astrocytoma compared to 0% for those with high-grade astrocytoma. While 5 out of 11 patients under the age of 25 years at their initial presentation have survived for from 2-16 years after the diagnosis, all patients presenting after the age of 25 years died within 3 years after treatment. Thalamic glial tumours are not a homogeneous group of tumours in terms of clinical behaviour and histopathological features, and the poor overall results, especially in adult tumours, thus emphasise the need for continued research in the treatment of these tumours.


Assuntos
Idade de Início , Neoplasias Encefálicas/patologia , Glioma/patologia , Tálamo/patologia , Adolescente , Adulto , Fatores Etários , Idoso , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade
10.
Clin Neurol Neurosurg ; 97(4): 300-6, 1995 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-8599896

RESUMO

Over the past 25 years, we have treated 17 patients with chiasmo-hypothalamic astrocytomas. Before 1988, the initial treatments consisted of surgery and/or radiotherapy, while since 1989, 4 children (1 male, 3 females, aged 3-8 years) were treated primarily with chemotherapy. None of them was associated with neurofibromatosis. After a biopsy of the tumor, the intravenous administration of ranimustine (MCNU; 30-86 mg/m2) and/or nimustine (ACNU; 30.3-64.1 mg/m2) was given without radiation therapy. Chemotherapy was usually given as an out-patient, with a total of 5-13 courses. The total doses of MCNU and ACNU administered ranged from 150 to 570 mg and from 64.8 mg to 100 mg, respectively. After chemotherapy 2 patients showed clinical improvement and tumor regression on neuro-imaging, while one patient showed clinical improvement and tumor size stabilization on neuro-imaging. The remaining one child, however, showed a clinical worsening and tumor progression on neuro-imaging studies. He was thus treated with a second chemotherapy regimen with carboplatin and etoposide, which brought about tumor regression. The acute and subacute toxicity of chemotherapy was mild. All patients are now leading almost normal lives with a median of 43 months after diagnosis. Although a longer and more careful clinical observation is required, the authors conclude that chemotherapy with MCNU and/or ACNU may benefit patients with unresectable pilocytic astrocytoma requiring treatment. The advantages of this therapy include its mild side effects and the lack of any hospitalization in most patients. It may also delay the need for radiation therapy, which can have a deleterious effect on the young developing brain.


Assuntos
Antineoplásicos/uso terapêutico , Astrocitoma/tratamento farmacológico , Neoplasias Encefálicas/tratamento farmacológico , Carboplatina/uso terapêutico , Quimioterapia Adjuvante , Etoposídeo/uso terapêutico , Hipotálamo/patologia , Nimustina/uso terapêutico , Compostos de Nitrosoureia/uso terapêutico , Quiasma Óptico/patologia , Antineoplásicos/administração & dosagem , Astrocitoma/patologia , Neoplasias Encefálicas/patologia , Carboplatina/administração & dosagem , Etoposídeo/administração & dosagem , Feminino , Humanos , Lactente , Imageamento por Ressonância Magnética , Masculino , Nimustina/administração & dosagem , Compostos de Nitrosoureia/administração & dosagem
11.
Anaesthesia ; 48(5): 390-2, 1993 May.
Artigo em Inglês | MEDLINE | ID: mdl-8317646

RESUMO

The analgesic effects of transdermally applied 10% lignocaine aquagel containing 3% glycyrrhetinic acid monohemiphthalate disodium (as an absorption enhancer) and EMLA cream were compared on the forearms of 34 adult volunteers in a double-blind fashion. The mean pinprick pain scores (graded by noting the number of painful pinpricks out of five) at 30, 60 and 90 min after application and 30 min after removal of the anaesthetics were 3.3 (0.3) (mean SE), 1.2 (0.3), 0.3 (0.1) and 0.3 (0.1) respectively, in the lignocaine gel group. Corresponding scores were 3.5 (0.3), 1.5 (0.3), 0.7 (0.2) and 0.1 (0.1) respectively, in the EMLA group. Insertion of a 26-gauge needle into the treated skin to a depth of 1 mm at 90 min after application was not painful in 91% of the volunteers in the lignocaine gel group and 88% of those in the EMLA group. There was no significant difference in any of the corresponding pain scores between the two groups.


Assuntos
Anestesia Local , Lidocaína , Administração Cutânea , Adulto , Anestésicos Locais , Método Duplo-Cego , Combinação de Medicamentos , Feminino , Humanos , Lidocaína/administração & dosagem , Combinação Lidocaína e Prilocaína , Masculino , Pessoa de Meia-Idade , Dor/prevenção & controle , Medição da Dor , Prilocaína , Absorção Cutânea/efeitos dos fármacos , Fatores de Tempo
12.
Am J Kidney Dis ; 20(5): 489-91, 1992 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-1442760

RESUMO

Clinical application of dermal patch anesthesia to relieve pain at venous cannulation of blood-access was studied in hemodialysis patients. Aqueous gel of 10% lidocaine base with 3% glycyrrhetinic acid monohemiphthalate disodium (GA MHPh 2Na) was applied for 60 minutes to the skin of the patients. Degree of pain was expressed as a pain score. Analgesic effect of the lidocaine gel was evaluated in 16 patients in a placebo-controlled, double-blind, cross-over design by comparing the gel with lidocaine with a placebo gel without lidocaine. The mean pin-prick pain score (1.0 +/- 0.5) in the lidocaine gel patch (n = 16) was significantly lower than that (2.3 +/- 0.3) in the placebo gel patch (P < 0.01). In 8.8% of the patients, blood pressure was elevated after venous cannulation, but this tendency was modified by dermal patch anesthesia with the lidocaine gel. Plasma concentration of lidocaine was under the detection limit of assay (< 0.05 micrograms/mL) after dermal patch anesthesia in six subsequent dialysis treatments.


Assuntos
Anestesia Local/métodos , Sangria , Cateterismo Periférico , Lidocaína/administração & dosagem , Diálise Renal , Administração Cutânea , Adulto , Anestesia Local/instrumentação , Pressão Sanguínea , Método Duplo-Cego , Feminino , Géis , Humanos , Lidocaína/sangue , Masculino , Pessoa de Meia-Idade , Medição da Dor , Placebos
13.
Anesth Analg ; 75(4): 555-7, 1992 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-1530169

RESUMO

Three types of skin pretreatments (stripping with adhesive tape, scrubbing with Skin-Pure jelly, and cleaning with benzine) were studied in 21 volunteers to determine whether the pretreatments could shorten the application period of transdermal 3% GA MHPh 2Na-10% lidocaine gel. Before application of the gel, each subject received skin pretreatment on the volar surface of one forearm. Then, the lidocaine gel soaked in a round sponge was applied on both the pretreated area of the forearm and the unpretreated contrast area of the other forearm. A pain score was obtained from each subject at 30, 45, 60, and 75 min after placement of the dermal patch by noting the number of painful pinpricks of five delivered. The mean pain scores on the pretreated side were significantly lower at 30, 45, and 60 min in the stripping study group and at 45 and 60 min in the cleaning study group, compared with the corresponding scores on the unpretreated side (P less than 0.05). Skin pretreatments, such as stripping and cleaning, are useful for shortening the onset of the dermal patch anesthesia.


Assuntos
Anestesia Local/métodos , Ácido Glicirretínico/farmacologia , Lidocaína/administração & dosagem , Absorção Cutânea , Administração Cutânea , Adulto , Géis , Ácido Glicirretínico/análogos & derivados , Humanos , Masculino , Pessoa de Meia-Idade , Absorção Cutânea/efeitos dos fármacos
14.
Anaesthesia ; 47(8): 708-10, 1992 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-1519725

RESUMO

The clinical efficacy of transdermal 10% lignocaine gel mixture containing 3% w/w glycyrrhetinic acid monohemiphthalate disodium as an absorption promoter was evaluated at venous cannulation in 17 paediatric (6-11 years) and 17 adult (29-65 years) patients. After about 60 min of occlusive application, the mean (SD) pinprick pain score was 0.7 (0.7) in the paediatric group, compared with 1.4 (1.3) in the adults (p less than 0.05). Twenty-five patients (14 children and 11 adults) who had a pinprick score of 0 or 1 underwent venous cannulation without intradermal local anaesthetic. The mean (SD) pain scores at venepuncture showed no significant differences between children and adults.


Assuntos
Anestesia Local , Ácido Glicirretínico/farmacologia , Lidocaína , Administração Cutânea , Adulto , Idoso , Cateterismo Periférico , Criança , Feminino , Géis , Humanos , Masculino , Pessoa de Meia-Idade , Absorção Cutânea/efeitos dos fármacos
15.
Fukuoka Igaku Zasshi ; 83(4): 174-80, 1992 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-1607143

RESUMO

The effects of green tea extract on caries inhibition of hamsters and on acid resistance of human tooth enamel have been investigated. Both in vivo and in vitro experiments showed that original extract of green tea had the significant effects on these points. The dialyzed tea solution in which the fluoride was removed almost completely, also showed the remarkable effects both in vivo and in vitro experiment as is similar to the original tea extract. The results obtained from this study suggested that fluoride in green tea may play a role to increase the cariostatic action in cooperation with other components in tea. However, the action of fluoride does not seem to be so important, because its concentration is very low. The effect of green tea on caries inhibition as well as on the increment of acid resistance appears to be more correlative with the non-dialysable substances in tea.


Assuntos
Cárie Dentária/prevenção & controle , Chá , Animais , Cricetinae , Suscetibilidade à Cárie Dentária , Humanos , Técnicas In Vitro , Masculino , Mesocricetus
16.
J Neurol ; 238(8): 427-32, 1991 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-1779248

RESUMO

Auditory middle-latency responses were recorded in 45 neurological patients. In 4 patients with localized lesions in the thalamus, or subcortical white matter, the component Na was attenuated or delayed at T3, Cz and T4, exclusively on stimulation of the ear contralateral to the lesion. In 5 patients with temporal lobe lesions with receptive aphasia, the amplitude of component Pa was decreased on the lesion side (T3), regardless of the side of stimulation. These findings suggest that Na is generated from a subcortical structure and Pa is generated from the temporal cortex. In 25 patients with diffuse CNS lesions, V-Na interpeak latency was prolonged in 2 cases.


Assuntos
Encefalopatias/fisiopatologia , Tronco Encefálico/fisiopatologia , Potenciais Evocados Auditivos , Lobo Temporal/fisiopatologia , Tálamo/fisiopatologia , Adolescente , Adulto , Afasia de Broca/etiologia , Afasia de Broca/fisiopatologia , Afasia de Wernicke/etiologia , Afasia de Wernicke/fisiopatologia , Córtex Auditivo/fisiopatologia , Encefalopatias/classificação , Encefalopatias/complicações , Dominância Cerebral , Feminino , Lobo Frontal/fisiopatologia , Corpos Geniculados/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade
17.
Neurosurgery ; 28(2): 223-9; discussion 229-30, 1991 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-1997890

RESUMO

To improve the localization of stereotactic targets, somatosensory evoked potentials (SEPs) were recorded from the thalamus and subthalamic area using a specially designed semimicroelectrode in 61 patients and a conventional "macroelectrode" in 17 patients. By means of the semimicroelectrode, median nerve stimulation evoked two distinct SEPs, consisting of a diphasic wave with a huge positivity restricted to the nucleus ventrocaudalis (Vc) and a triphasic wave of lower amplitude with a major negativity in the ventral part of the nucleus ventrointermedius (Vim) and nucleus ventrooralis posterior (Vop) as well as the subthalamic lemniscal pathway. The Vim-Vc junction could thus be clearly delineated by an abrupt transition of SEPs from one type to the other with a precision of 1 mm. The parvicellular part of the Vc (Vcpc), situated in its basal region, was distinguishable from the Vc proper by a significant reduction of the positivity elicited by stimulation of the median nerve and by a rapid growth of a diphasic SEPs to stimulation of the posterior tibial nerve. In the other thalamic nuclei, stimulation of the median nerve elicited triphasic SEPs of a very small amplitude, suggesting a volume conduction current from the lemniscal pathway. With the macroelectrode, the positivity in the Vc was sensitive to electrode manipulation and the thalamic nuclei could not be distinctly outlined. SEP monitoring using the semimicroelectrode significantly improved the precision of target localization, which allowed minimizing of the volume of the therapeutic lesion without losing surgical effectiveness, while avoiding complications associated with increased penetration of the coagulating electrode. It is suggested that recording serial thalamic SEPs with the semimicroelectrode is a practical method to refine stereotactic targets in the thalamus.


Assuntos
Potenciais Somatossensoriais Evocados/fisiologia , Microeletrodos , Transtornos dos Movimentos/fisiopatologia , Dor Intratável/fisiopatologia , Técnicas Estereotáxicas , Tálamo/fisiologia , Adulto , Idoso , Estimulação Elétrica , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Transtornos dos Movimentos/cirurgia , Dor Intratável/cirurgia , Tempo de Reação
18.
Artigo em Inglês | MEDLINE | ID: mdl-2470574

RESUMO

The distribution and generator sources of somatosensory evoked potentials (SEPs) in the thalamus and subthalamic area were studied, using a 'semi-microelectrode' during stereotaxic surgery on 34 patients with involuntary movements or intractable pain. Electrical stimulation was given to the median nerve at the wrist. Two distinct SEPs were evoked by contralateral stimulation. A high voltage (160 microV) positive SEP with a peak latency of 15.5 msec was strictly confined to the ventral part of the sensory relay nucleus (nucleus ventro-caudalis, V.c). A much lower voltage, positive-negative-positive triphasic SEP showed peak latencies of the initial positivity and the major negativity of 13.3 msec and 16.0 msec, respectively, and had maximal voltage (16 microV) in the ventralmost parts of the nucleus ventro-intermedius (V.im) and radiatio praelemniscalis (Ra.prl), and substantial potentials in the lemniscus medialis (L.m) and nucleus ventro-oralis posterior (V.o.p). The potential field of the triphasic SEP spread farther across the different thalamic nuclei and subthalamic region with identical configurations and peak latencies, but with decreasing amplitude. These findings suggest that the high voltage positive SEP reflects a postsynaptic potential generated by the V.c neurons, and the smaller triphasic SEP a presynaptic axonal potential generated in the rostral part of the lemniscal pathway, extending by means of volume conduction.


Assuntos
Potenciais Somatossensoriais Evocados , Tálamo/fisiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Mapeamento Encefálico , Estimulação Elétrica , Feminino , Humanos , Masculino , Nervo Mediano/fisiologia , Microeletrodos , Pessoa de Meia-Idade , Doenças do Sistema Nervoso/fisiopatologia , Tempo de Reação , Técnicas Estereotáxicas
19.
Arch Oral Biol ; 27(10): 809-16, 1982.
Artigo em Inglês | MEDLINE | ID: mdl-6961893

RESUMO

Condensed phosphates (CP: Na salts of pyro-, tripoly-, tetrapoly-, pentapoly-, hexameta- and ultra-; K salts of pyro- and poly-) used as food additives depressed the growth of seven strains of Streptococcus mutans (serotype a-g) as assessed by disc diffusion methods. Minimal inhibitory concentrations (MIC) of CP on growth of strain K1-R (g) in a chemically defined medium were measured turbidimetrically. Commercial grade CP (CP used) had the same growth inhibitory effects as purified linear type CP and they were superior to the cyclic type CP. The MIC of CP for Strep. mutans appeared to be related to their chelating capacity. As the growth inhibition by CP was reversed by the addition of divalent metal ions (Ca2+, Mg2+ or Mn2+), the chelating capacity of CP was apparently responsible for their antibacterial action. The antibacterial action of CP seemed largely bacteriostatic. Condensed phosphates depressed lactate production from glucose and sucrose by the cells of strain K1-R. A similar inhibition was produced by the supernatant of heated human saliva. Condensed phosphates depressed insoluble glucan production from sucrose by the cells of strain K1-R. The inhibition of sugar metabolism may be due to the interference of sugar transport into Strep. mutans induced by the chelation effects of CP. Hamsters were inoculated orally with strain K1-R and reared on the high-sucrose diet No. 2000 supplemented with 2 per cent (w/w) CP for 60 days. Dietary supplements of CP were associated with reduced caries activity (p less than 0.01) and plaque formation (p less than 0.05). The antibacterial actions of CP could be responsible for these caries-inhibitory effects.


Assuntos
Cárie Dentária/prevenção & controle , Fosfatos/farmacologia , Streptococcus mutans/efeitos dos fármacos , Animais , Cricetinae , Placa Dentária/prevenção & controle , Depressão Química , Glucose/metabolismo , Mesocricetus , Testes de Sensibilidade Microbiana , Streptococcus mutans/metabolismo , Sacarose/metabolismo
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