Dihydro-ß-agarofuran-type sesquiterpenoids from the seeds of Celastrus virens with lifespan-extending effect on the nematode Caenorhabditis elegans.

Twelve dihydro-ß-agarofuran-type sesquiterpenoids, including five new ones (1-5), were purified from the seeds of Celastrus virens (Wang et Tang) C. Y. Chent et T. C. Kao. Their chemical structures were characterized via comprehensive spectroscopic analysis, single-crystal X-ray diffraction analysis, and computational prediction of ECD, as well as comparison of observed and reported NMR spectral data. Among the isolates, nine abundant dihydro-ß-agarofuran-type sesquiterpenoids were evaluated for their lifespan-extending activity using the nematode Caenorhabditis elegans model. As a result, compounds 1, 2, 5, 6, 8, and 9 (50 µM) significantly extended the mean survival time of C. elegans, respectively, compared with the blank control group (p < 0.05). Further Quantitative RT-PCR showed that the prolonging of lifespan mediated by compounds 1, 6, 8, and 9 were dependent on the transcription factors skn-1 and hsf-1.

Diterpenoids from the seeds of Euphorbia lathyris and their effects on microglial nitric oxide production.

Four new lathyrane-type diterpenoids (1-4) and a novel macrocyclic diterpenoid (5) featuring a 5/7/7/4-fused ring system, together with seventeen known ones (6-22), were isolated from the seeds of Euphorbia lathyris. Their structures were elucidated by extensive spectroscopic analyses and single crystal X-ray crystallography. These isolates were evaluated for their inhibition against nitric oxide (NO) production induced by lipopolysaccharide (LPS) in BV-2 microglial cells. As a result, the inhibitory rates of compounds 1, 3, 4, 6, 7, 9-11, 13-15, 20, and 21 on NO production were more than 40% with the cell viability more than 80% at their effective concentrations. In addition, compounds 6 and 11 markedly reduced the mRNA levels of pro-inflammatory cytokines IL-6 and IL-1ß in LPS-stimulated BV-2 cells.