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1.
Protoplasma ; 216(3-4): 143-54, 2001.
Artigo em Inglês | MEDLINE | ID: mdl-11732182

RESUMO

We characterized the behavior of plastid (pt) and mitochondrial (mt) nucleoids during male gametogenesis in Plumbago auriculata in three dimensions. The behavior of pt-nucleoids and mt-nucleoids differed throughout male gametogenesis. Pt-nucleoids were distributed in a characteristic manner in three stages: in the early microspore, pt-nucleoids assemble around cell nucleus; in the mid-generative cell, pt-nucleoids gather at the internal side of the pollen; in the late-generative cell, pt-nucleoids aggregation turns its pole to the external side of the pollen. We also studied organelle nucleoids in the egg and the central cell by a method in which semi-thick sections of resin-embedded anthers and ovaries were observed by confocal laser scanning microscopy. The number of pt-nucleoids in the sperm cell did not differ significantly from that in the egg. These results suggest that the behavior of DNA-containing organelles is regulated strictly during male gametogenesis in P. auriculata, and that a biparental inheritance of plastids in the Plumbago embryo is more favored than was previously thought.


Assuntos
Magnoliopsida/fisiologia , Mitocôndrias/metabolismo , Plastídeos/metabolismo , Núcleo Celular/metabolismo , DNA de Plantas/metabolismo , Hibridização In Situ , Magnoliopsida/genética , Mitocôndrias/ultraestrutura , Modelos Biológicos , Estruturas Vegetais/citologia , Plastídeos/ultraestrutura , Pólen/ultraestrutura , Reprodução
2.
J Obstet Gynaecol Res ; 27(1): 33-5, 2001 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-11330728

RESUMO

In the presence of severe hyperbilirubinemia in Crigler-Najjar syndrome Type II, a fetus is at risk for kernicterus. A 34-year-old woman, gravida 4, para 1, with Crigler-Najjar syndrome Type II was treated with phenobarbital administration following phototherapy during each of 2 pregnancies. Both infants were healthy and developed normally.


Assuntos
Anticonvulsivantes/uso terapêutico , Síndrome de Crigler-Najjar/terapia , Fenobarbital/uso terapêutico , Fototerapia , Complicações na Gravidez/terapia , Adulto , Feminino , Humanos , Recém-Nascido , Gravidez , Resultado da Gravidez
3.
Brain Dev ; 22(5): 315-20, 2000 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10891639

RESUMO

OBJECTIVE: To know the effect of brief-repetitive intermittent hypoxia-ischemia on the development of perinatal brain damage. STUDY DESIGN: Seven-day-old Wistar rats underwent ligation of the unilateral common carotid artery. The animals were allocated to three groups (n=12 in each group) and exposed to 8% oxygen as follows: group A: continuous exposure for 180 min; group B: continuous exposure for 90 min; and group C: 10 min of exposure repeated at 10-min intervals over a period of 180 min (total exposure time, 90 min). Seventy-two hours after exposure to hypoxia, the cerebral cortex was examined to assess the degree of neuronal necrosis and brain damage was classified into four grades of severity, 0-3. To evaluate the extent of brain damage, we used immunohistochemical staining with TIB-128 antibody, which reacts to MAC-1 antigen specific to microglia, and observed the glial reaction in the cerebral cortex, hippocampus, thalamus, and striatum. RESULTS: All the brain damage observed in groups A-C occurred on the side where the ligation was performed. The most severe damage was found in group A animals, of which seven showed significant neuronal necrosis, having a grade 2 or more advanced lesion. In group B, neuronal necrosis was modest, with only one animal having a grade 2 lesion. In group C, a significant neuronal necrosis was found in six animals despite having the same period of hypoxic exposure as those in group B. MAC-1 positive cells appeared in the cerebral cortex of histologically damaged animals and extended to the hippocampus, thalamus, and striatum in severely damaged animals from groups A, B, and C. CONCLUSION: Examination of the neonatal rat model suggested that repetitive and intermittent, rather than continuous hypoxia-ischemia, causes pronounced damage in the immature brain.


Assuntos
Encéfalo/patologia , Hipóxia-Isquemia Encefálica/patologia , Animais , Animais Recém-Nascidos , Encéfalo/irrigação sanguínea , Encéfalo/metabolismo , Artéria Carótida Primitiva/fisiologia , Artéria Carótida Primitiva/cirurgia , Córtex Cerebral/metabolismo , Córtex Cerebral/patologia , Corpo Estriado/metabolismo , Corpo Estriado/patologia , Gliose/patologia , Hipocampo/metabolismo , Hipocampo/patologia , Hipóxia-Isquemia Encefálica/classificação , Imuno-Histoquímica , Ácido Láctico/metabolismo , Ligadura , Antígeno de Macrófago 1/metabolismo , Ratos , Ratos Wistar , Índice de Gravidade de Doença , Tálamo/metabolismo , Tálamo/patologia
4.
Planta ; 209(1): 53-65, 1999 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-10467031

RESUMO

Organellar DNA in mature pollen grains of eight angiosperm species (Actinidia deliciosa Lindl., Antirrhinum majus L., Arabidopsis thaliana (L.) Heynh., Medicago sativa L., Musa acuminata Colla, Pelargonium zonale (L.) L'Hér, Petunia hybrida Vilm. and Rhododendron mucronatum (Blume) G. Don, in which the modes of organellar inheritance have been determined genetically, was observed by fluorescence microscopy using Technovit 7100 resin sections double-stained with 4',6-diamidino-2-phenylindole (DAPI) and 3,3'-dihexyloxacarbocyanine iodide (DiOC(6)). The eight species were classified into four types, based on the presence or absence of organellar DNA in mature generative cells: namely (1) type "m+p+", which has both mitochondrial and plastid DNA (P. zonale), (2) type "m+p-", which only has mitochondrial DNA (M. acuminata), (3) type "m-p+", which only has plastid DNA (A. deliciosa, M. sativa, R. mucronatum), and (4) type "m-p-", which has neither mitochondrial nor plastid DNA (A. majus, A. thaliana, P. hybrida). This classification corresponded to the mode of organellar inheritance determined by genetic analysis. The presence or absence of mitochondrial and plastid DNA corresponded to paternal/biparental inheritance or maternal inheritance of the respective organelle, respectively. When organellar DNA was present in mature generative cells (m+ or p+), the DNA content of the organelles in the generative cells started to increase immediately after pollen mitosis one (PMI). In contrast, the DNA content of organelles in generative cells decreased rapidly after PMI when organellar DNA was absent from mature generative cells (m- or p-). These results indicate that the modes of inheritance (paternal/biparental inheritance or maternal inheritance) of mitochondria and plastids are determined independently of each other in young generative cells just after PMI.


Assuntos
DNA de Plantas , Mitose , Organelas/genética , Pólen/genética , Citoplasma , DNA Mitocondrial , Herança Extracromossômica , Plantas/classificação , Plantas/genética , Pólen/crescimento & desenvolvimento
5.
Eur J Cell Biol ; 78(4): 241-8, 1999 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-10350212

RESUMO

The amount of organellar DNA in a generative cell of Pharbitis nil was observed when squashed pollen grains collected on the day of flowering were stained with the DNA-specific fluorochrome 4',6-diamidino-2-phenylindole (DAPI). Using both DAPI-fluorescence microscopy and electron microscopy, observation of the same thin section of Technovit 7100 resin-embedded material revealed that all of the organellar DNA in mature generative cells is plastid DNA, and there is no mitochondrial DNA. During pollen development, we observed organellar DNA in fluorescence microscopic images using double-staining with DAPI and 3,3'-dihexyloxacarbocyanine iodide (DiOC6) and quantified the DNA using a video-intensified microscope photon counting system (VIMPCS). In the vegetative cells, the amounts of both mitochondrial and plastid DNA progressively decreased and had disappeared by 2 days before flowering. In the generative cells, mitochondrial DNA disappeared sooner than in the vegetative cells, indicating a more active mechanism for the decrease in mitochondrial DNA in the generative cells. In contrast, plastid DNA in the generative cells increased markedly. The DNA content per plastid was at a minimum value (corresponding to one copy of the plastid genome) 7 days before flowering, but it increased to a maximum value (corresponding to over 10 copies of the plastid genome) 2 days before flowering. Similar results were also obtained with immunogold electron microscopy using an anti-DNA antibody. These results suggest that the DNA content of mitochondria and plastids in P. nil is controlled independently during pollen development.


Assuntos
DNA Mitocondrial/metabolismo , Proteínas de Plantas/metabolismo , Plastídeos/metabolismo , Pólen/citologia , Pólen/metabolismo , Imuno-Histoquímica , Indóis/metabolismo , Mitose , Fatores de Tempo
6.
Environ Health Perspect ; 107(6): 495-500, 1999 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-10339451

RESUMO

To investigate which parameters are stimulated by mineral fibers and whether cigarette smoke enhanced a fiber-induced response, we examined the level of cytokine mRNA from alveolar macrophages (AMs) and lungs of rats exposed to mineral fibers and cigarette smoke in vivo. Male Wistar rats were given a single intratracheal instillation of 2 mg of Union Internationale Contre le Cancer chrysotile or refractory ceramic fiber (RF1). The animals then inhaled a side stream of smoke 5 days per week for 4 weeks. The expression of manganese superoxide dismutase, inducible nitric oxide synthase (iNOS), basic fibroblast growth factor (bFGF), interleukin-1[alpha] (IL-1[alpha]), interleukin-6 (IL-6), and tumor necrosis factor-[alpha] (TNF[alpha]) mRNA from lipopolysaccharide-stimulated AMs and lungs of rats exposed to mineral fibers and/or cigarette smoke were assessed using semiquantitative reverse-transcriptase polymerase chain reaction. Exposure only to cigarette smoke increased in IL-1[alpha] mRNA levels in AMs. Chrysotile stimulated the expression of IL-1[alpha], TNF[alpha], and IL-6 in AMs, and the expression of bFGF in lungs. RF1 resulted in increased expression of IL-1[alpha] and TNF[alpha] in AMs. Cigarette smoke stimulated the gene expression of iNOS in AMs and IL-6 and bFGF in lungs treated with chrysotile; IL-1[alpha] in AMs and bFGF in lungs did the same in lungs with RF1. Among these cytokines, message levels of IL-1[alpha], iNOS, and bFGF were increased in rats stimulated with mineral fibers, and the stimulating effects of mineral fibers were enhanced by cigarette smoke. Therefore, IL-1[alpha], iNOS, and bFGF would be the possible parameters of the lung remodeling induced by mineral fibers.


Assuntos
Citocinas/biossíntese , Regulação da Expressão Gênica/efeitos dos fármacos , Fibras Minerais/toxicidade , Nicotiana , Plantas Tóxicas , RNA Mensageiro/biossíntese , Fumaça/efeitos adversos , Animais , Líquido da Lavagem Broncoalveolar/citologia , Primers do DNA , DNA Complementar/biossíntese , DNA Complementar/isolamento & purificação , Sinergismo Farmacológico , Inibidores Enzimáticos/toxicidade , Etídio/toxicidade , Pulmão/patologia , Masculino , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase Via Transcriptase Reversa
7.
Pflugers Arch ; 435(6): 767-74, 1998 May.
Artigo em Inglês | MEDLINE | ID: mdl-9518504

RESUMO

The aim of the current study was to elucidate the synergism of dietary calcium restriction and exhaustive exercise in the antioxidant enzyme system of rat soleus muscle, and to investigate the involvement of neutrophils in exercise-induced muscle damage. Forty-eight male Wistar rats were assigned to the following groups: control (C) or calcium-restricted [1 month (1 M) or 3 months (3 M)]. Each group was subdivided into acutely exercised or non-exercised groups. Soleus muscle from each rat was analysed to determine the levels of antioxidant enzymes [Mn-superoxide dismutase (SOD), Cu, Zn-SOD, glutathione peroxidase (GPX), and catalase (CAT)]. Dietary calcium restriction resulted in calcium deficiency and upregulated the antioxidant enzymes examined except GPX. Conversely, exhaustive exercise significantly decreased GPX and CAT, but not SODs activities in the calcium-restricted (1 M and/or 3 M) rats. Contents of immunoreactive Mn-SOD and Cu,Zn-SOD were only increased in the 3 M rats. During calcium restriction, the mRNA expression of both forms of SOD showed initial upregulation, followed by downregulation. Exhaustive exercise significantly increased the mRNA expressions only in the 3 M rats. Moreover, exhaustive exercise markedly increased myeloperoxidase activity in soleus muscles from the 1 M and 3 M rats compared with the C rats, and significantly enhanced the ability of neutrophils to generate superoxide in the 3 M rats. The results demonstrate that dietary calcium restriction upregulates certain antioxidant enzyme activities in rat soleus muscle, indicating an enhanced resistance to potential increases in intracellular reactive oxygen species. The results also suggest that exhaustive exercise may cause oxidative damage in soleus muscle of calcium-deficient rats through the activation of neutrophils.


Assuntos
Antioxidantes/metabolismo , Cálcio/deficiência , Músculo Esquelético/enzimologia , Esforço Físico/fisiologia , Animais , Peso Corporal , Cálcio/sangue , Cálcio da Dieta/administração & dosagem , Catalase/metabolismo , Glutationa Peroxidase/metabolismo , Masculino , Músculo Esquelético/anatomia & histologia , Neutrófilos/metabolismo , Tamanho do Órgão , Peroxidase/metabolismo , Fósforo/sangue , Resistência Física , RNA Mensageiro/metabolismo , Ratos , Ratos Wistar , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismo , Superóxidos/metabolismo
8.
Eur J Pharmacol ; 333(2-3): 231-40, 1997 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-9314040

RESUMO

We examined the mechanism of the inflammatory response induced by topical application of mustard oil (0.5-20.0%/20 microliters per ear) to the mouse ear compared to that of the response to capsaicin. The dose-dependent increases in plasma extravasation and ear thickness reached a maximum at approximately 30 min after mustard oil application. Topical pretreatment of ears with capsaicin (250 micrograms/ear) diminished mustard oil-induced plasma extravasation for up to day 7 but not at day 14 after treatment. However, desensitization of the exudative response was not evoked by reapplication of mustard oil to ears. The inflammatory response to mustard oil did not differ between the ears of mast cell-deficient mice and those of the congenic normal mice. Mustard oil-induced plasma extravasation was unaffected by pretreatment with histamine H1 and 5-HT2 receptor antagonists and the capsaicin-functional inhibitor, ruthenium red, which inhibit capsaicin-induced ear oedema. The endopeptidase inhibitor, phosphoramidon, enhanced the ability of mustard oil to increase dye leakage. The tachykinin NK1 receptor antagonist, SR 140333 ((S)1-[2-[3-(3,4-dichlorophenyl)-1-(3-isopropoxyphenylacetyl)pi peridin-3-yl]ethyl]-4-phenyl-1-azoniabicyclo[2.2.2.]octane, chloride), not only inhibited mustard oil-induced plasma extravasation but also blocked the enhancement by phosphoramidon of the response to mustard oil. In contrast, the tachykinin NK2 receptor antagonist, SR 48968 ((S)-N-methyl-N[4-(4-acetylamino-4-phenylpiperidino)-2-(3,4,- dichlorophenyl)butyl]benzamide), and the tachykinin NK3 receptor antagonist, SR 142801 ((S)-(N)-(1-(3-(1-benzoyl-3-(3,4-dichlorophenyl)piperidin-3-yl)pro pyl)-4- phenylpiperidin-4-yl)-N-methylacetamide), had no effect on plasma extravasation. The present results demonstrated that mustard oil induces mouse skin inflammation through a mechanism different from that for capsaicin. Mediators such as histamine and 5-HT from mast cells appear to be minor factors in the response to mustard oil. In addition, evidence supports the assumption that the tachykinin NK1 receptor is involved in this model.


Assuntos
Dermatite/etiologia , Edema/induzido quimicamente , Mostardeira/toxicidade , Extratos Vegetais/toxicidade , Plantas Medicinais , Administração Tópica , Animais , Permeabilidade Capilar/efeitos dos fármacos , Capsaicina/administração & dosagem , Dermatite/patologia , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Orelha , Edema/tratamento farmacológico , Antagonistas dos Receptores Histamínicos H1/farmacologia , Indicadores e Reagentes/farmacologia , Masculino , Mastócitos , Camundongos , Óleos de Plantas , Inibidores de Proteases/farmacologia , Inibidores de Proteases/uso terapêutico , Receptores de Taquicininas/antagonistas & inibidores , Rutênio Vermelho/farmacologia , Antagonistas da Serotonina/farmacologia
9.
Inflamm Res ; 45(6): 303-7, 1996 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-8814463

RESUMO

We examined the effect of SR 140333, a nonpeptide NK1 receptor antagonist, FK 888, a peptide NK1 antagonist, and SR 142801, a non-peptide NK3 antagonist, on ear oedema induced by topical application of capsaicin (250 micrograms/ear) in mice. SR 140333 (ED50:39 micrograms/kg, i.v.) dose-dependently inhibited the oedema response to capsaicin, whereas FK 888 (1.0 mg/kg, i.v.) and SR 142801 (3.0 mg/kg, i.v.) had no effect. Furthermore, SR 140333 significantly (p < 0.001) suppressed ear oedema in response to intradermal injection of substance P (SP) (100 pmol/site) by i.v. administration (0.1 mg/kg,) and co-injection (50 pmol/site). In contrast, FK 888 (1.0 mg/kg, i.v. and 500 pmol/site) was ineffective in the response to SP. The present results suggest that the difference in effects of the two NK1 receptor antagonists on the oedema response to capsaicin is due to species differences in affinities for the NK1 receptor in the mouse skin. Moreover, it seems unlikely that the NK3 receptor is involved primarily in capsaicin-induced mouse ear oedema.


Assuntos
Dipeptídeos/uso terapêutico , Edema/tratamento farmacológico , Indóis/uso terapêutico , Piperidinas/uso terapêutico , Quinuclidinas/uso terapêutico , Receptores de Taquicininas/antagonistas & inibidores , Animais , Sítios de Ligação , Capsaicina/administração & dosagem , Capsaicina/toxicidade , Dipeptídeos/administração & dosagem , Dipeptídeos/farmacologia , Modelos Animais de Doenças , Otopatias/tratamento farmacológico , Edema/induzido quimicamente , Indóis/administração & dosagem , Indóis/farmacologia , Injeções Intravenosas , Dose Letal Mediana , Masculino , Camundongos , Piperidinas/administração & dosagem , Piperidinas/farmacologia , Quinuclidinas/administração & dosagem , Quinuclidinas/farmacologia , Pele/efeitos dos fármacos , Pele/metabolismo , Estereoisomerismo , Relação Estrutura-Atividade , Substância P/administração & dosagem , Substância P/toxicidade
11.
Biochem Biophys Res Commun ; 219(2): 486-91, 1996 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-8605014

RESUMO

A cDNA encoding human phospholipid hydroperoxide glutathione peroxidase (PHGPx) was obtained by PCR amplification from human testis cDNA and was inserted into the plasmid pRc/CMV to construct an expression vector for human PHGPx. Guinea pig cell line 104C1 cells were transfected with the expression vector. One of the transfectants, designated 104Cl/O4C, expressed high glutathione peroxidase activity toward dilinoleoyl phosphatidylcholine hydroperoxide and linoleic acid hydroperoxide. Western blot analysis revealed a large amount of protein immunoreactive against anti-PHGPx antibody in the transfectant. When the cells were incubated with these hydroperoxides, the parental cells suffered from serious cell injury, whereas the transfectant was extremely resistant against lipid hydroperoxide-mediated injury.


Assuntos
Sobrevivência Celular/efeitos dos fármacos , Expressão Gênica/efeitos dos fármacos , Glutationa Peroxidase/biossíntese , Ácidos Linoleicos/toxicidade , Peróxidos Lipídicos/toxicidade , Fosfatidilcolinas/toxicidade , Animais , Sequência de Bases , Western Blotting , Linhagem Celular , DNA Complementar , Glutationa Peroxidase/genética , Cobaias , Humanos , Masculino , Dados de Sequência Molecular , Fosfolipídeo Hidroperóxido Glutationa Peroxidase , Plasmídeos , Reação em Cadeia da Polimerase , Proteínas Recombinantes/biossíntese , Testículo/enzimologia , Transfecção
12.
Inflamm Res ; 44(3): 125-30, 1995 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-7552577

RESUMO

We have investigated the effects of actinomycin D on mouse ear oedema induced by capsaicin, neuropeptides, and established inflammatory mediators. Actinomycin D (0.5 mg/kg, i.v.) significantly (P < 0.01) inhibited ear oedema induced by topical application of capsaicin, while adriamycin (6.0 mg/kg, i.v.) and cycloheximide (6.0 mg/kg, i.v.) had no effect on oedema. The ear oedema induced by intradermal injection of neuropeptides such as mammalian tachykinins, calcitonin gene-related peptide (CGRP), and vasoactive intestinal peptide (VIP), was markedly (P < 0.05, P < 0.01 or P < 0.001) suppressed by actinomycin D. The drug was also effective (P < 0.01 or P < 0.001) in inhibiting bradykinin (BK)- and compound 48/80-induced ear oedema, but did not inhibit oedema induced by histamine, 5-HT, leukotriene C4 (LTC4), and platelet activating factor (PAF) at a dose of 1 mg/kg. In mast cell-deficient W/WV mice, actinomycin D (1.0 mg/kg, i.v.) failed to inhibit substance P (SP)-induced ear oedema whereas spantide (0.5 mg/kg, i.v.) was an effective (P < 0.01) inhibitor of oedema formation. Furthermore, actinomycin D (10-100 microM) dose-dependently prevented histamine release from rat peritoneal mast cells evoked by SP, compound 48/80, and the ionophore A23182, respectively. These results strongly suggest that an inhibitory effect of actinomycin D on neurogenic inflammation is due primarily to the prevention of mast cell activation mediated by neuropeptides, rather than an interaction with DNA or receptors of neuropeptides.


Assuntos
Dactinomicina/uso terapêutico , Edema/tratamento farmacológico , Animais , Bradicinina/administração & dosagem , Bradicinina/toxicidade , Calcimicina/administração & dosagem , Calcimicina/toxicidade , Peptídeo Relacionado com Gene de Calcitonina/administração & dosagem , Peptídeo Relacionado com Gene de Calcitonina/toxicidade , Capsaicina/administração & dosagem , Capsaicina/toxicidade , Cicloeximida/administração & dosagem , Cicloeximida/toxicidade , Dactinomicina/administração & dosagem , Dactinomicina/farmacologia , Modelos Animais de Doenças , Relação Dose-Resposta a Droga , Doxorrubicina/administração & dosagem , Doxorrubicina/uso terapêutico , Otopatias/tratamento farmacológico , Edema/induzido quimicamente , Histamina/administração & dosagem , Histamina/metabolismo , Histamina/toxicidade , Injeções Intravenosas , Leucotrieno C4/administração & dosagem , Leucotrieno C4/toxicidade , Masculino , Mastócitos/citologia , Mastócitos/efeitos dos fármacos , Camundongos , Fator de Ativação de Plaquetas/administração & dosagem , Fator de Ativação de Plaquetas/toxicidade , Ratos , Ratos Wistar , Serotonina/administração & dosagem , Serotonina/toxicidade , Substância P/administração & dosagem , Substância P/toxicidade , Taquicininas/administração & dosagem , Taquicininas/toxicidade , Peptídeo Intestinal Vasoativo/administração & dosagem , Peptídeo Intestinal Vasoativo/toxicidade , p-Metoxi-N-metilfenetilamina/administração & dosagem , p-Metoxi-N-metilfenetilamina/toxicidade
13.
Rinsho Shinkeigaku ; 34(4): 356-60, 1994 Apr.
Artigo em Japonês | MEDLINE | ID: mdl-8026130

RESUMO

We report a case of a 54-year-old man with sudden-onset double-vision. On admission, neurological examination showed upward and downward gaze palsy on voluntary and smooth pursuit movements, and vertical oculocephalic maneuver elicited a full upward and downward response. Bell's phenomenon, horizontal eye movements and convergence were not impaired. Based on these findings, supranuclear dissociated vertical gaze palsy was diagnosed. T1-weighted MR images revealed low intensity on the medial side of the right thalamo-mesencephalic junction, which impaired the rostral interstitial nucleus of the medial longitudinal fasciculus (riMLF). T2-weighted MR images revealed a high-intensity area. The posterior commissure was spared. The clinical signs gradually improved, and the vertical gaze palsy almost disappeared one month after onset. Based on these findings, unilateral infarct in the thalamo-mesencephalic junction in the distribution of the right paramedian thalamic artery was diagnosed. Only two cases of upward and downward gaze palsy in association with unilateral upper midbrain lesion without posterior commissure have been previously reported. Since we did not perform a pathological examination, we cannot deny that there may have been some denervation of fibers at the posterior commissure. Cases of upward and downward gaze palsy in association with unilateral upper midbrain lesion without posterior commissure are rare, and it is very interesting that the lesion in our patients, like that seen in the two pathological reports, was right-sided.


Assuntos
Infarto Cerebral/complicações , Mesencéfalo/irrigação sanguínea , Oftalmoplegia/etiologia , Paralisia Supranuclear Progressiva/etiologia , Tálamo/irrigação sanguínea , Infarto Cerebral/diagnóstico , Humanos , Imageamento por Ressonância Magnética , Masculino , Pessoa de Meia-Idade
14.
Gan To Kagaku Ryoho ; 20(12): 1857-60, 1993 Sep.
Artigo em Japonês | MEDLINE | ID: mdl-8397490

RESUMO

We encountered a case of partial response in a patient with multiple liver metastases from gastric cancer as a result of treatment by hepatic artery infusions of epirubicin/mitomycin C-Lipiodol suspension. A 56-year-old man was diagnosed as having advanced gastric cancer with multiple liver metastases. Subtotal gastrectomy with regional lymph node dissection and cholecystectomy were performed, and an injection port was implanted in the proper hepatic artery. Ten days after the operation, the intermittent chemotherapies were performed, and 3 months after the effect was judged to be a partial response by CT scan. Severe side effects were not seen during treatment, and the man is now healthy and working for more than one year after the operation. Thus, this regimen may be found useful for patients with liver metastases.


Assuntos
Adenocarcinoma Papilar/tratamento farmacológico , Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem , Bombas de Infusão Implantáveis , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Gástricas/patologia , Adenocarcinoma Papilar/secundário , Quimioterapia Adjuvante , Colecistectomia , Preparações de Ação Retardada , Epirubicina/administração & dosagem , Gastrectomia , Humanos , Infusões Intra-Arteriais , Óleo Iodado/administração & dosagem , Neoplasias Hepáticas/secundário , Excisão de Linfonodo , Masculino , Pessoa de Meia-Idade , Mitomicina/administração & dosagem , Neoplasias Gástricas/cirurgia
15.
Masui ; 42(8): 1157-61, 1993 Aug.
Artigo em Japonês | MEDLINE | ID: mdl-8366555

RESUMO

Hypotensive anesthesia induced by trimethaphan was maintained at 70-79 mmHg of the systolic radial artery pressure for two hours in 35 patients undergoing spherical acetabul osteotomy. The creatinine clearance, free water clearance, fractional sodium excretion, urine volume, urinary excretion of NAG and levels of gamma-GTP, serum GOT and GPT were measured perioperatively. The creatinine clearance, free water clearance and urine volume decreased by 50% during surgery but recovered to normal values on the third day after surgery. The fractional sodium excretion remained unchanged during and after surgery. The urinary NAG activity increased from 3 +/- a (SD) to 10 +/- 5 U.g-1 and gamma-GTP activity increased from 23 +/- 7 to 33 +/- 13 U.g-1 during surgery, and these remained at high levels for three days after surgery. The serum GOT and GPT levels were within normal ranges for 21 postoperative days. The increase in urinary enzyme activities during and after surgery indicates that mild damage of renal tubular cells has occurred during hypotensive anesthesia.


Assuntos
Hipotensão Controlada , Rim/fisiopatologia , Fígado/fisiopatologia , Adolescente , Adulto , Anestesia Geral , Pressão Sanguínea , Transfusão de Sangue Autóloga , Feminino , Humanos , Testes de Função Renal , Masculino , Pessoa de Meia-Idade , Osteotomia , Trimetafano
16.
J Immunol ; 139(2): 411-6, 1987 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-3110270

RESUMO

To elucidate the pathogenesis of immunological diseases induced by the drug D-Penicillamine (D-Pen) the requirements for sensitization to this drug were investigated. Mice were subcutaneously (s.c.) injected into one hind footpad with a solution of D-Pen without adjuvant, and reactivity to D-Pen was determined in the popliteal lymph node assay (PLNA) by weight increase of the draining PLN, the incorporation of 3H-thymidine, and trapping of 51Cr-labeled syngeneic lymphocytes in the draining PLN. The peak of the primary PLN response was obtained between day 7 and 10 after injecting 1 mg of D-Pen per mouse. Likewise, PLN enlargement could be induced by injecting 18 hr nonadherent spleen cells s.c. that had been pretreated overnight with D-Pen in vitro. D-Pen-induced PLN enlargement was primarily caused by cell proliferation within the lymph node, and only a minor portion was due to trapping of circulating lymphocytes. The majority of the cells in the enlarged PLN were B cells; T cells, however, were required for generation of PLN enlargement. For induction of PLN reactivity to D-Pen, the stereoisomer L-Pen, and the dimer D-Pen disulfide, it was mandatory that the respective molecules were administered in ionized form. PLN reactivity to D-Pen is controlled by at least two loci, one mapping to the I region, possibly A beta A alpha, the other(s) to the non-H-2 background. As far as studied, high responsiveness was inherited dominantly. The PLN reaction proved to be antigen-specific, since D-Pen-primed mice exhibited an enhanced reaction when challenged with a suboptimal dose of D-Pen, but not when challenged with an unrelated drug, diphenylhydantoin (DPH). The possible relationship between immunity to D-Pen and autoimmunity induced by this drug is discussed.


Assuntos
Genes MHC da Classe II , Linfonodos/imunologia , Penicilamina/imunologia , Linfócitos T/imunologia , Animais , Movimento Celular , Relação Dose-Resposta Imunológica , Memória Imunológica , Linfonodos/patologia , Ativação Linfocitária , Complexo Principal de Histocompatibilidade , Camundongos , Camundongos Endogâmicos , Relação Estrutura-Atividade
17.
J Immunol ; 136(1): 136-42, 1986 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-2415613

RESUMO

A mouse model for assessing the sensitization of T cells to the drug D-penicillamine (D-Pen) has been established. Mice were primed with D-Pen in vivo and the secondary response of specific T cells was measured in a proliferative assay in vitro. Priming was achieved by injecting either free D-Pen or, more effectively, D-Pen in complete Freund's adjuvant at the base of the tail. After 7 to 9 days, the draining lymph node cells (responder cells) were restimulated in vitro with syngeneic spleen cells that had been preincubated with D-Pen for 20 to 22 hr and washed (D-Pen-SC). The thiol (-SH) group of D-Pen was required for generating effective stimulator cells; other thiol compounds, however, or heavy metals (Hg, Au) were unable to generate cross-reacting stimulators on incubation with spleen cells. Although D-Pen-SC proved to be good stimulator cells, thymocytes and mouse erythrocytes, after having been preincubated with D-Pen, completely failed to stimulate. The proliferating responder cells were Lyt-1+2- T cells that were highly specific for D-Pen-SC and were even capable of distinguishing between the two stereoisomers D-Pen and L-Pen. The splenic T cells of recipient mice were effectively primed by injecting D-Pen-SC, but not free D-Pen, via the i.v. route. These findings indicate that D-Pen can act as a hapten for specific T cells when presented on the surface of appropriate stimulator cells. Conceivably, if D-Pen were able to generate effective stimulator cells in vivo, this could lead to T cell reactions comparable to those in the graft-vs-host reaction (GVHR). We discuss the possibility that the GVH-like adverse immunologic side-effects of D-Pen in patients may have a GVHR-like pathogenesis.


Assuntos
Ativação Linfocitária/efeitos dos fármacos , Penicilamina/farmacologia , Baço/imunologia , Linfócitos T/imunologia , Animais , Antígenos Ly , Antígenos de Grupos Sanguíneos/imunologia , Relação Dose-Resposta Imunológica , Epitopos/imunologia , Injeções Intravenosas , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos C57BL , Fenótipo , Baço/citologia , Baço/transplante , Estereoisomerismo , Compostos de Sulfidrila/farmacologia , Linfócitos T/classificação , Timo/citologia
18.
J Pediatr Gastroenterol Nutr ; 4(2): 268-73, 1985 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-3157790

RESUMO

Hepatic ornithine carbamoyltransferase (OCT) and alcohol dehydrogenase (ADH) activities were measured in six groups of rats: (A) fed a severe zinc-(Zn-) deficient diet (1.98 ppm) for 5 weeks; (B) pair-fed control for group (A); (C) fed a less severe Zn-deficient diet (6.10 ppm) for 5 weeks; (D) pair-fed control for group (C); (E) fed a Zn-supplemented control diet (90.4 ppm) for 5 weeks; and (F) first fed the severe Zn-deficient diet for 5 weeks and then replaced on the Zn-supplemented control diet until a body weight corresponding to the final weight of group (E) was obtained. Hepatic OCT was similar in all these six groups. On the contrary, hepatic ADH was significantly reduced in groups (A) and (C) and in each of the corresponding pair-fed groups, (B) and (D). No differences were found between groups (A) and (B) or between groups (C) and (D). In group (F), ADH activity improved to a level equivalent to that in group (E). The changes in ADH activities were accompanied by changes in the hepatic Zn content. Thus, it is clear that: (1) the hepatic Zn content may not be affected by the amount of Zn intake alone, but by the combination of Zn and food intake; and (2) ADH, and not OCT, reflected the hepatic Zn content.


Assuntos
Oxirredutases do Álcool/metabolismo , Fígado/enzimologia , Ornitina Carbamoiltransferase/metabolismo , Zinco/deficiência , Álcool Desidrogenase , Aminoácidos/sangue , Animais , Peso Corporal , Ingestão de Alimentos , Fígado/análise , Masculino , Ratos , Ratos Endogâmicos , Ureia/metabolismo
19.
Tohoku J Exp Med ; 132(4): 385-8, 1980 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-7256724

RESUMO

A case is reported of a 5-year-old boy with Lowe syndrome. The patient was unusual in that he had only mild and transient acidosis with no rickets. The corneal opacities in the parents suggest that this disease might be autosomal recessive.


Assuntos
Síndrome Oculocerebrorrenal/sangue , Erros Inatos do Transporte Tubular Renal/sangue , Bicarbonatos/sangue , Pré-Escolar , Humanos , Concentração de Íons de Hidrogênio , Masculino , Fósforo/sangue
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