Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Mais filtros

Base de dados
Ano de publicação
Tipo de documento
Intervalo de ano de publicação
1.
Gene ; 543(2): 253-8, 2014 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-24704275

RESUMO

Acetyl CoA Carboxylase 1 (ACC1) is a biotin-dependent enzyme that catalyzes the carboxylation of Acetyl CoA to form Malonyl CoA, the key intermediate metabolite in fatty acid synthesis. In this study, the mRNA expression of the ACC1 gene was evaluated in four different tissues (liver, visceral fat, subcutaneous fat, and longissimus muscle) of the domestic goat (Capra hircus) kids feeding on four different levels of trivalent chromium (0, 0.5, 1, and 1.5mg/day) as food supplementation. RT-qPCR technique was used for expression analyses and heat shock protein 90 gene (HSP-90) was considered as reference gene for data normalization. Our results revealed that 1.5mg/day chromium significantly reduced the expression of the ACC1 gene in liver, visceral fat, and subcutaneous fat tissues, but not in longissimus muscles (P<0.05). We measured some phenotypic traits of kid's carcasses to detect their probable correlations with chromium-mediated downregulation of ACC1 expression. Interestingly, changes in ACC1 expression were accompanied with decreased accumulation of fats in adipose tissues such that the subcutaneous fat thickness and heart fat percentage decreased in kids feeding on chromium. By contrast, chromium supplemented kids showed higher percentage of muscles despite the fact that their total body weight did not differ from that of non-supplemented kids. Our study suggests that trivalent chromium alters the direction of energy accumulation towards muscles rather than fats and provides insights into application of chromium supplementation as a useful strategy for improvement of meat quality in domestic animals.


Assuntos
Acetil-CoA Carboxilase/genética , Cromo/administração & dosagem , Suplementos Nutricionais , Cabras/genética , Carne , Melhoria de Qualidade , Animais , Regulação para Baixo , Gordura Intra-Abdominal/enzimologia , Lipogênese/genética , Fígado/enzimologia , Masculino , Músculo Esquelético/enzimologia , Gordura Subcutânea/enzimologia
2.
Biol Trace Elem Res ; 156(1-3): 124-9, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24078326

RESUMO

Chromium is a biologically important element for humans and laboratory animals. Although the favorable effects of trivalent chromiumon immune responses of studied animals have been well documented, the precise mechanisms by which the chromium acts on immune system is relatively poor studied. In this study, real-time qPCR technique was employed to evaluate the expression profiles of four immune-related genes (B2M, MHCA, MHCB, and Rap2A) in spleens of the domestic goats, Capra hircus, feeding on four different levels of supplemental chromium (0, 0.5, 1, and 1.5 mg/day) as chromium­ methionine. The results showed that 1.5 mg/day of supplemental chromium significantly increased the expression of the four studied genes (P <0.01). Since the studied genes play important roles in development, activation, and migration of lymphocytes, their increased expression seems to be an unknown mechanism by which chromium impose reinforcing effects on immune system. Therefore, supplemental chromium can be potentially used to improve immune responses especially in animals experiencing any type of stress such as invasion by a pathogen.


Assuntos
Compostos de Cromo/farmacologia , Suplementos Nutricionais , Regulação da Expressão Gênica/efeitos dos fármacos , Cabras/imunologia , Antígenos de Histocompatibilidade/imunologia , Baço/imunologia , Animais , Feminino , Regulação da Expressão Gênica/imunologia , Humanos , Linfócitos/imunologia , Masculino
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA