Sustained oxidation of Tea-Fe(III)/H2O2 simultaneously achieves sludge reduction and carbamazepine removal: The crucial role of EPS regulation.

Establishing an economic and sustained Fenton oxidation system to enhance sludge dewaterability and carbamazepine (CBZ) removal rate is a crucial path to simultaneously achieve sludge reduction and harmless. Leveraging the principles akin to "tea making", we harnessed tea waste to continually release tea polyphenols (TP), thus effectively maintaining high level of oxidation efficiency through the sustained Fenton reaction. The results illustrated that the incorporation of tea waste yielded more favorable outcomes in terms of water content reduction and CBZ removal compared to direct TP addition within the Fe(III)/hydrogen peroxide (H2O2) system. Concomitantly, this process mainly generated hydroxyl radical (•OH) via three oxidation pathways, effectively altering the properties of extracellular polymeric substances (EPS) and promoting the degradation of CBZ from the sludge mixture. The interval addition of Fe(III) and H2O2 heightened extracellular oxidation efficacy, promoting the desorption and removal of CBZ. The degradation of EPS prompted the transformation of bound water to free water, while the formation of larger channels drove the discharge of water. This work achieved the concept of treating waste with waste through using tea waste to treat sludge, meanwhile, can provide ideas for subsequent sludge harmless disposal.

Involvement of PI3K/Akt/ß-catenin signaling in schisandrin B-mitigated bone deterioration in an experimental rat model of estrogen deficiency.

Introduction: Schisandrin B (SchB) has been reported to perform a wide range of biological functions, including antioxidant activity, anti-inflammatory activity and stimulation of osteoblast proliferation. However, the function and mechanism of SchB in ovariectomy (OVX)-induced osteoporosis are still unknown. The present study was designed to investigate the anti-osteoporotic activity of SchB in an experimental rat model of estrogen deficiency, which is usually used to mimic human postmenopausal osteoporosis (PMO). Material and methods: OVX rats were orally treated with low (10 mg/kg) or high (50 mg/kg) doses of SchB for 8 weeks. Bone metabolism-related markers were measured by ELISA. The levels of protein expression were determined by western blotting analysis. Hematoxylin and eosin (H&E) and safranin O staining were performed to analyze trabecular bone and cartilage degeneration. Tartrate-resistant acid phosphatase (TRAP) staining was used to evaluate osteoclast differentiation. Results: SchB administration markedly increased serum Ca levels and bone Ca content and decreased urinary calcium excretion in OVX-operated rats. In addition, high-dosage SchB treatment blocked osteoclastogenesis and improved trabecular bone and cartilage degeneration in the tibia of OVX-operated rats. Furthermore, high-dosage SchB treatment dramatically elevated the protein expression of phospho-PI3K, phospho-Akt and ß-catenin in OVX-operated rats. Conclusions: SchB exerted anti-osteoporotic activity in OVX-operated rats by accelerating the phosphorylation of PI3K and Akt, subsequently upregulating the expression of ß-catenin.

Novel three-dimensional Ti3C2-MXene embedded zirconium alginate aerogel adsorbent for efficient phosphate removal in water.

Excessive phosphorus in water causes environmental security problems like eutrophication. Advanced two-dimensional material MXene has attracted raising attention in aquatic adsorption, while lack of selectivity and difficult recovery limit its application in phosphate removal. In this study, Ti3C2-MXene embedded zirconium-crosslinked SA (MX-ZrSA) beads were synthesized and their phosphate adsorption performance under different conditions was assessed. Investigations using SEM/EDS, XRD, BET, TGA and contact angle meter reveal that the addition of Ti3C2-MXene enhanced the thermal stability, mechanical strength, hydrophilicity, and formed loose network-like mesoporous inner structure with large surface area. The theoretical maximum adsorption capacity was 492.55 mg P/g and was well fitted by Freundlich and optimized Langmuir models. The Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy analysis showed that chemisorption was involved, and the formation of Zr-O-P and Ti-O-P complexes accounted for high selectivity and affinity to phosphate. The adsorption experiments in real waters and lab-scale continuous flow Anaerobic-Anoxic-Oxic reactor further indicated the application potential of MX-ZrSA beads. Our study will provide insight into MXene and SA aerogel synergistic adsorption of aquatic contaminants and help with the removal and recovery of finite phosphorus resource.

Cathode potential regulates the microbiome assembly and function in electrostimulated bio- dechlorination system.

Mechanism of microbiome assembly and function driven by cathode potential in electro-stimulated microbial reductive dechlorination system remain poorly understood. Here, core microbiome structure, interaction, function and assembly regulating by cathode potential were investigated in a 2,4,6-trichlorophenol bio-dechlorination system. The highest dechlorination rate (24.30 µM/d) was observed under - 0.36 V with phenol as a major end metabolite, while, lower (-0.56 V) or higher (0.04 V or -0.16 V) potentials resulted in 1.3-3.8 times decreased of dechlorination kinetic constant. The lower the cathode potential, the higher the generated CH4, revealing cathode participated in hydrogenotrophic methanogenesis. Taxonomic and functional structure of core microbiome significantly shifted within groups of - 0.36 V and - 0.56 V, with dechlorinators (Desulfitobacterium, Dehalobacter), fermenters (norank_f_Propionibacteriaceae, Dysgonomonas) and methanogen (Methanosarcina) highly enriched, and the more positive interactions between functional genera were found. The lowest number of nodes and links and the highest positive correlations were observed among constructed sub-networks classified by function, revealing simplified and strengthened cooperation of functional genera driven by group of - 0.36 V. Cathode potential plays one important driver controlling core microbiome assembly, and the low potentials drove the assembly of major dechlorinating, methanogenic and electro-active genera to be more deterministic, while, the major fermenting genera were mostly governed by stochastic processes.

Comparative Efficacy of Xianling Gubao Capsules in Improving Bone Mineral Density in Postmenopausal Osteoporosis: A Network Meta-Analysis.

OBJECTIVE: The clinical efficacy of Xianling Gubao capsule (XLGB) and its combination therapy in the treatment of postmenopausal osteoporosis (PMOP) was systematically evaluated by frequency-based network meta-analysis. METHODS: We searched the China National Knowledge Infrastructure (CNKI), Wanfang, SinoMed, PubMed, Embase and Cochrane Library databases to identify clinical trials of XLGB for the treatment of PMOP from the establishment of each database to November 22, 2021. The quality of the included studies was evaluated by using the risk of bias assessment tool version 2.0 (Rob 2.0) recommended by Cochrane. Stata 14.0 was applied for statistical analysis of the data, and the surface under the cumulative ranking curve (SUCRA) was used to rank the intervention measures of each outcome index. RESULTS: This study included 22 clinical trials (including 19 RCTs and 3 non-RCTs) involving 12 drug therapies. According to the results of the network meta-analysis and SUCRA, the best three interventions for improving lumbar bone mineral density (BMD) are XLGB+BP+calcium (83.7%), XLGB+BP (68.5.7%) and XLGB+VD (67.1%). XLGB+calcium was the best combination regimen for improving femoral neck BMD and increasing bone Gla protein (BGP) and alkaline phosphatase (ALP) contents in serum. The SUCRA values of XLGB+calcium for improving the three outcome indicators were 68.0%, 59.5% and 82.1%, respectively. CONCLUSIONS: The results of this network meta-analysis show that combined application of XLGB can effectively improve BMD and serum BGP and ALP compared to calcium alone, VD or BP. In the future, multicenter, large-sample and double-blind clinical RCTs should be carried out to supplement and verify the results of this study.

Effects of side-stream operation on the mainstream biological phosphorus metabolic pathway for phosphorus recovery: Simulation by an extended ASM2d model.

An extended activated sludge model (E-ASM2d) was established by including the metabolic processes of double-layer extracellular polymeric substances (EPS) and glycogen-accumulating organisms (GAOs) into the existing ASM2d model for describing and predicting the metabolic processes of the side-stream phosphorus (P) recovery reactor. A sensitivity analysis of model parameters on SPO4(soluble phosphate), XLEPS (loosely-bound EPS), XTEPS (tightly-bound EPS), COD, and SNH4 (soluble ammonia nitrogen) outputs was conducted for identifying influential parameters. The predicted effluent values of COD, ammonia nitrogen (NH4), and P corresponded well with actual measured values and all the model performance coefficient values for COD, NH4, and P were higher than 0.65, implying the E-ASM2d model could accurately simulate the metabolic processes of the side-stream P recovery process under different COD:P ratio conditions. The variations in the mainstream biological P metabolic pathway under different COD:P conditions were investigated by the E-ASM2d model. At COD:P ratios of 30, 20, and 10, the values of fPP,TEPS (fraction of XTEPS in polyphosphate metabolic process) increased from 0.092, 0.094, and 0.096 in the initial phase to 0.107, 0.124, and 0.187 in the side-stream phase, respectively, demonstrating that the fraction of P removal by tightly-bound EPS was improved by the side-stream operation.

Life cycle assessment of sewage sludge treatment and disposal based on nutrient and energy recovery: A review.

With the acceleration of urbanization, the production of urban sludge is increasing rapidly. To minimize resource input and waste output, it is crucial to execute analyses of environmental impact and assessments of sustainability on different technical strategies involving sludge disposal based on Life Cycle Assessment (LCA), which is a great potential mean of environmental management adopted internationally in the 21st century. This review aims to compare the environmental sustainability of existing sludge management schemes with a purpose of nutrient recovery and energy saving, respectively, and also to include the substitution benefits of alternative sludge products. Simultaneously, LCA research regarding the emerging sludge management technologies and sludge recycling (cement, adsorbent, bricks) is analyzed. Additionally, the key aspects of the LCA process are worth noting in the context of the current limitations reviewed here. It is worth emphasizing that no technical remediation method can reduce all environmental damage simultaneously, and these schemes are typically more applicable to the assumed local conditions. Future LCA research should pay more attention to the toxic effects of different sludge treatment methods, evaluate the technical ways of adding pretreatment technology to the 'front end' of the sludge treatment process, and further explore how to markedly reduce environmental damage in order to maximize energy and nutrient recovery from the LCA perspective.

Development of a double-layer EPS-ASM2d model to illustrate the effect on mainstream biological phosphorus system in side-stream phosphorus recovery process.

In order to deeply investigate the influences of side-stream phosphorus (P) recovery operation on mainstream biological P removal system, an improved activated sludge model no. 2 (ASM2d) was established to illuminate the metabolic processes of P in a side-stream P recovery reactor. The improved ASM2d (named D-EPS-ASM2d) was established by extending of the P metabolic processes of double-layer extracellular polymeric substances (EPS) into conventional ASM2d model. The predicted effluent concentrations of COD, NH4, and TP by the D-EPS-ASM2d had good fits with measured values in the side-stream P recovery process. Comparing with conventional ASM2d, the likelihood values of D-EPS-ASM2d related to COD, NH4, and TP effluents were increased from 0.694, 0.837 and 0.762 to 0.868, 0.904 and 0.920, respectively, implying the simulation performances of D-EPS-ASM2d on nutrient removal processes were significantly improved. Besides, the calibrated values of fPP,TEPS was 0.09, 0.102 and 0.123 as side-stream volume (SSV) increasing from 0.3 to 0.9, implying the fraction of P removal by tightly-bound EPS was enhanced with the increase of SSV.

Meta-Analysis of Elderly Lower Body Strength: Different Effects of Tai Chi Exercise on the Knee Joint-Related Muscle Groups.

OBJECTIVE: To determine whether lower body strength such as keen extension and flexion strength may be improved by Tai Chi exercise in older adults from the perspective of evidence-based medicine. METHODS: Databases of PubMed, Embase, and Cochrane Library were searched up to July 1, 2021. Randomized clinical trials are adopted to compare Tai Chi exercise with sedentary behavior or other low intensity exercise in terms of influence on lower body strength rehabilitation, especially keen extension and flexion strength in people aged over 60. A meta-analysis was performed to discuss outcomes of lower body strength, knee muscle strength, and knee extension/flexion strength. RESULTS: A total of 25 randomized trials involving 1995 participants fulfilled the inclusion criteria. (1) Tai Chi exercise significantly improved elderly lower body strength (-0.54, [-0.81, -0.28], p < 0.00001, I 2 = 74%), but there was no differential improvement in the strength of the knee joints (0.10, [-0.02, 0.23], p=0.11, I 2 = 34%). (2) Elderly individual lower body strength declined with age, while this trend was suppressed by Tai Chi exercise (-0.35, [0.14, 0.56], p=0.001, I 2 = 70%). (3) Although Tai Chi exercise did no significantly improve the large muscle group of knee joint extensor like quadriceps femoris (3.15, [-0.69, 6.99], p=0.24, I 2 = 26%), it showed marked enhancement to the strength of deep small muscle group of knee joint flexor (10.25, [6.90, 13.61], p < 0.00001, I 2 = 0%). The heterogeneity might be caused by distinguished measurements of muscle strength. Therefore, Tai Chi exercise specifically enhanced some certain muscle strength of knee joints and improved muscle fitness rehabilitation as well as function activity for elderly. CONCLUSIONS: In this RCT meta-analysis, Tai Chi exercise has positive effects on lower body strength of elderly. Although no obvious improvement on the knee extensor is observed, it may be used as a rehabilitation treatment for training stable deep muscle groups to improve the knee flexion strength significantly.

Effects of various side stream phosphorus recovery volume on the performance and microbial structures of mainstream biological system.

The effects of three side stream phosphorous recovery volume on the performance and microbial structure of biological phosphorous removal system were investigated. Results showed that the removal of COD and nitrogen had no significant impacts by side stream operation, but the removal of phosphorous were gradually enhanced with the increase in side stream volume (SSV). The secretion of extracellular polymeric substance (EPS) were variously promoted at the stripping period. However, with the increase in SSV, the inhibition on EPS and phosphorus-accumulating organisms (PAOs) phosphorous absorption were severe and the restoration were tougher. The high throughout 16S rRNA gene sequencing revealed the succession of microbial population were significantly effected by side stream operation. The relative abundances of PAOs reduced to 0.17%, 0.09% and 0.07% with 30%, 60% and 90% side stream operations, respectively. At the restoration period, the relative abundance could restore to 95.4%, 65% and 38% initial values, respectively. The relative abundances of glycogen-accumulating organisms were variously enhanced under various SSV conditions. In conclusion, at SSV of 60%, more abundant recovered phosphorous could be obtained and had slighter and reversible effects on activated sludges. The SSV of 60% was the applicable SSV for phosphorous recovery from the biological phosphorus removal system.

La-based-adsorbents for efficient biological phosphorus treatment of wastewater: Synergistically strengthen of chemical and biological removal.

The present work demonstrated the invention of synergistically strengthen of chemical and biological removal of phosphorus (P) in biological wastewater treatment, which was achieved by exposure the bioreactors to different levels of La-based-adsorbents. We fabricated a high-performance La2O2CO3 micro-adsorbent (H-La2O2CO3) and added it into sequencing batch reactors. When activated sludge was exposed to 40 mg/L H-La2O2CO3 for 40 d, effluent total phosphorus (TP) concentration significantly decreased to approximately 0.18 mg/L, with the steady removal efficiency of 96.4%, which is superior to the biological phosphorus removal (BPR). The effect of H-La2O2CO3 dosages on P removal in biological wastewater treatment was also detailedly investigated. The H-La2O2CO3 adsorbent could not only capture P by chemical bonding itself, but also increased protein (PN) contents of extracellular polymeric substances (EPS) and changed the functional group of EPS to chemically adsorb P. Additionally, the results of 16s rDNA molecular analysis revealed that the species richness and microbial diversity varied with the different dosages of adsorbent. Sequence analyses showed that the appropriate concentration of H-La2O2CO3 addition increased the contents of several polyphosphate accumulating organisms (PAOs) at genus level in sludge.

The effect of supporting matrix on sludge granulation under low hydraulic shear force: Performance, microbial community dynamics and microorganisms migration.

Granular sludge usually takes extracellular polymers (EPS) as matrices for colonizing microorganisms and maintaining structural stability. However, the low strength of EPS threatens the disintegration of granules, especially under low hydraulic shear force. To accelerate the formation and enhance the stability of granules, micro-sized melamine (ME) sponges (RA) and polyurethane (PU) sponges (RB) were screened out as matrix substitutes for developing aerobic granular biofilm (AGB) in this study. The superficial gas velocity was 0.8 cm s-1. Both reactors achieved over 95% ammonium nitrogen removal efficiency within 10 days. During stabilization period, the chemical oxygen demand, total nitrogen and total phosphorus removal efficiencies were 90.5%, 70% and 95% in RA and 87.8%, 83% and 88% in RB, respectively. Confocal laser scanning microscopy (CLSM) detection revealed that ß-polysaccharide was more concentrated in the outer layer in PU-AGB but uniformly dispersed in ME-AGB. The denitrifying phosphorus accumulating organisms (Flavobacterium) was dominant in RA, while the denitrifying glycogen accumulating organisms (Candidatus_Competibacter) was dominant in RB. Fluorescence in situ hybridization (FISH) analysis indicated that the microbial distribution in ME-AGB was relatively uniform, while there was a significant migration of functional microorganisms in PU-AGB. The super-hydrophilicity of ME and the high hydrophobicity of PU may be the main reasons for these differences. Overall, this study indicated that ME sponge is a more suitable material for supporting AGB than PU sponge.

Start-up of aerobic granular biofilm at low temperature: Performance and microbial community dynamics.

Low temperature is a great challenge for the biological treatment of wastewater. In this study, the rapid start-up of aerobic granular biofilm (AGF) reactor was realized by adding micro-sized polyurethane (PU) sponges as matrices at 10 °C. The results showed that the granulation process of AGF was different from that of traditional aerobic granular sludge and biofilms, which was formed by using the sludge intercepted in PU matrix instead of sponge skeletons as granulation carriers. During the 5-month operation period, stable pollutants removal performance was achieved within 70 days, besides, the corresponding ammonium, total nitrogen, and total phosphorus removal efficiencies were 98%, 70%, and 95%, respectively. The addition of PU matrices inhibited the growth of filamentous bacteria and provided support for high structural stability of AGF. With the operation of the reactor, the relative abundance of traditional denitrifying bacteria (genera Thauera and Acidovorax, etc.) decreased gradually, and the putative denitrifying phosphorus accumulating genus, Dechloromonas, occupied a dominant position in the system. This experiment showed that AGF system could be successfully started-up and operated with efficient pollutants removal performance under low temperature when using micro-sized PU sponges as matrices.

Accelerated microbial reductive dechlorination of 2,4,6-trichlorophenol by weak electrical stimulation.

Microbial reductive dechlorination of chlorinated aromatics frequently suffers from the long dechlorination period and the generation of toxic metabolites. Biocathode bioelectrochemical systems were verified to be effective in the degradation of various refractory pollutants. However, the electrochemical and microbial related working mechanisms for bio-dechlorination by electro-stimulation remain poorly understood. In this study, we reported the significantly improved 2,4,6-trichlorophenol dechlorination activity through the weak electro-stimulation (cathode potential of -0.36 V vs. SHE), as evidenced by the 3.1 times higher dechlorination rate and the complete dechlorination ability with phenol as the end dechlorination product. The high reductive dechlorination rate (20.8 µM/d) could be maintained by utilizing electrode as an effective electron donor (coulombic efficiency of 82.3 ±â€¯4.8%). Cyclic voltammetry analysis of the cathodic biofilm gave the direct evidences of the cathodic respiration with the improved and positive-shifted reduction peaks of 2,4,6-TCP, 2,4-DCP and 4-CP. The optimal 2,4,6-TCP reductive dechlorination rate (24.2 µM/d) was obtained when a small amount of lactate (2 mM) was added, and the generation of H2 and CH4 were accompanied due to the biological fermentation and methanogenesis. The electrical stimulation significantly altered the cathodic biofilm structure and composition with some potential dechlorinators (like Acetobacterium) predominated. The microbial interactions in the ecological network of cathodic biofilm were more simplified than the planktonic community. However, some potential dechlorinators (Acetobacterium, Desulfovibrio, etc.) shared more positive interactions. The co-existence and possible cooperative relationships between potential dechlorinators and fermenters (Sedimentibacter, etc.) were revealed. Meanwhile, the competitive interrelations between potential dechlorinators and methanogens (Methanomassiliicoccus) were found. In the network of plankton, the fermenters and methanogens possessed the more positive interrelations. Electro-stimulation at the cathodic potential of -0.36 V selectively enhanced the dechlorination function, while it showed little influence on either fermentation or methanogenesis process. The study gave suggestions for the enhanced bioremediation of chlorinated aromatics, in views of the electro-stimulation capacity, efficiency and microbial interrelations related microbial mechanism.

A dynamic modelling of nutrient metabolism in a cyclic activated sludge technology (CAST) for treating low carbon source wastewater.

A new mathematical model incorporating biopolymer kinetics and the process of the simultaneous storage and growth are established for the treatment of low carbon source wastewater with a high effluent quality and energy efficiency. A set of initial parameter values was assigned as a combination of estimated values, literature-based values, and fitted values to simulate a cyclic activated sludge technology (CAST) system effectively. Compared with experimental data from the CAST system, the calibrated model demonstrated a good performance. Model simulations indicated that the recommended condition for a CAST fed with low carbon source wastewater was a volume ratio of the anoxic zone to the aerobic zone of 7/28. Moreover, using high-throughput 16S rRNA gene sequencing not only characterised the microbial communities in the CAST reactors operated under two feeding ratios but also indirectly validated the model predictions.

Sensitized chemiluminescence of 2-phenyl-4,5-di(2-furyl)-1H-imidazole/K3Fe(CN)6/propyl gallate system combining with solid-phase extraction for the determination of propyl gallate in edible oil.

In this paper, a novel chemiluminescence (CL) method has been developed for the determination of propyl gallate (PG). The proposed method was based on the enhancing effect of PG on the CL signal of 2-phenyl-4,5-di(2-furyl)-1H-imidazole (PDFI) and K3Fe(CN)6 reaction in an alkaline solution. Under the optimum conditions, the enhanced CL intensity was linearly related to the concentration of PG. The linear range of the calibration curve was 0.05-8 µg/mL, and the corresponding detection limit (3σ) was 0.036 µg/mL. The relative standard deviation for determining 1.0 µg/mL PG was 2.8% (n=11). The proposed method has been successfully applied to the determination of PG in edible oil. The edible oil samples were prepared by the solid-phase extraction (SPE) with a C18 column served as the stationary phase. Furthermore, the possible CL mechanism was also discussed briefly based on the photoluminescence (PL) and CL spectra.