Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 17 de 17
Filtrar
Mais filtros

Métodos Terapêuticos e Terapias MTCI
Base de dados
País/Região como assunto
Tipo de documento
País de afiliação
Intervalo de ano de publicação
1.
J Nat Med ; 75(3): 489-498, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33687660

RESUMO

New copaene-type and nerolidol-type sesquiterpenoids, 7-hydroxymustakone (1) and 15-hydroxynerolidol (2), and a 15-norlabdane diterpenoid, kaempcandiol (3), together with four known compounds (4-7) were isolated from the chloroform extract of Kaempferia candida roots and rhizomes. The structures of the new compounds 1-3 were elucidated based on 1D and 2D NMR and HRESIMS spectroscopic analyses. The extract of the K. candida roots and rhizomes and all isolated compounds 1-7 possessed HIV-1 viral protein R (Vpr) inhibitory activities on the TREx-HeLa-Vpr cell line at a 5 µM concentration, without detectable cytotoxicity.


Assuntos
Antivirais/farmacologia , Diterpenos/farmacologia , Sesquiterpenos/farmacologia , Zingiberaceae/química , Produtos do Gene vpr do Vírus da Imunodeficiência Humana/antagonistas & inibidores , Diterpenos/química , HIV-1/efeitos dos fármacos , Células HeLa , Humanos , Estrutura Molecular , Mianmar , Compostos Fitoquímicos/química , Compostos Fitoquímicos/farmacologia , Raízes de Plantas/química , Rizoma/química , Sesquiterpenos/química
2.
Fitoterapia ; 151: 104870, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-33652075

RESUMO

Six new isopimarane diterpenoids, shanpanootols A-F (1-6), along with two known analogues, were isolated from the ethyl acetate-soluble extract of Kaempferia pulchra rhizomes collected in Myanmar. The structures of these compounds were elucidated by extensive spectroscopic techniques such as 1D and 2D NMR and HRESIMS. The absolute configuration of 1 was determined by the modified Mosher method. The new isolates (1-6) were tested for their Vpr inhibitory activities against TREx-HeLa-Vpr cells. Shanpanootols C (3) and E (5) inhibited Vpr at doses of 2.5 and 5 µM, respectively.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Diterpenos/farmacologia , Zingiberaceae/química , Antineoplásicos Fitogênicos/isolamento & purificação , Diterpenos/isolamento & purificação , Produtos do Gene vpr/antagonistas & inibidores , Células HeLa , Humanos , Estrutura Molecular , Mianmar , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/farmacologia , Rizoma/química
3.
Fitoterapia ; 146: 104705, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32822767

RESUMO

Two new homodrimane sesquiterpenoids, globbatones A and B (1 and 2), and one 16-norlabdane diterpenoid, globbatone C (3), together with two new naturally occurring, (E)-labda-8(17),12-diene-15,16-olide (4) and γ-bicyclohomofarnesen-12-ol (5), and one known homodrimane sesquiterpenoid (6), nine known labdane diterpenoids (7-15), and one isospongian diterpenoid (16), were isolated from the chloroform extract of Globba sherwoodiana rhizomes. The structures of the new compounds 1-3 were elucidated based on 1D and 2D NMR and HRESIMS spectroscopic analyses. The chloroform extract of G. sherwoodiana rhizomes and 10 µM concentrations of some of its constituents 1, 3, 4, 8, 9, 12, and 14 showed the moderate anti-Vpr activities, without cytotoxic effects on the TREx-HeLa-Vpr cell line.


Assuntos
Fármacos Anti-HIV/farmacologia , Diterpenos/farmacologia , Sesquiterpenos/farmacologia , Zingiberaceae/química , Produtos do Gene vpr do Vírus da Imunodeficiência Humana/antagonistas & inibidores , Fármacos Anti-HIV/isolamento & purificação , Diterpenos/isolamento & purificação , Células HeLa , Humanos , Estrutura Molecular , Mianmar , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Extratos Vegetais/farmacologia , Rizoma/química , Sesquiterpenos/isolamento & purificação
4.
J Nat Med ; 74(3): 571-578, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32328863

RESUMO

Three new quassinoids, javanicinols A and B (1 and 2) and 4-keto-(16S)-methoxyjavanicin B (3), together with three known quassinoids (4-6) were isolated from the chloroform-soluble fraction of the methanol extract of the Picrasma javanica wood. The structures of 1-3 were determined by spectroscopic analyses, including 1D and 2D NMR, HRESIMS, and CD. The anti-HIV-1 viral protein R (Vpr) assay revealed that 1 and 2 exhibited potent anti-Vpr activities at 1.25 µM. Furthermore, the assay also revealed the potent anti-Vpr activities of (16R)-methoxyjavanicin B (7) and (16S)-methoxyjavanicin B (8), which were previously isolated from the Picrasma javanica wood.


Assuntos
Fármacos Anti-HIV/farmacologia , Produtos do Gene vpr/antagonistas & inibidores , HIV-1/efeitos dos fármacos , Picrasma/química , Quassinas/farmacologia , Fármacos Anti-HIV/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Quassinas/química , Quassinas/isolamento & purificação , Madeira/química
5.
J Nat Med ; 74(2): 487-494, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32006354

RESUMO

Kaempulchraols B-D (2-4), isopimara-8(9),15-diene diterpenoids isolated from Kaempferia pulchra rhizomes collected in Myanmar, were identified as potent NF-κB inhibitors. These compounds were also effective as NO inhibitory agents, with IC50 values of 47.69, 44.97, and 38.17 µM, respectively, without showing any cytotoxicity against LPS-induced RAW264.7 cells. Investigations of the mechanisms of action of 2-4 revealed that they inhibit the NF-κB-mediated transactivation of a luciferase reporter gene, IL-6 production, and COX-2 expression, with an effective dose of 25 µM. Thus, isopimarane diterpenoids are suggested to be potent inhibitors of NF-κB pathways and could be further explored as potential anti-inflammatory lead compounds.


Assuntos
Anti-Inflamatórios/uso terapêutico , Diterpenos/uso terapêutico , NF-kappa B/metabolismo , Rizoma/química , Zingiberaceae/química , Animais , Anti-Inflamatórios/farmacologia , Diterpenos/farmacologia , Camundongos
6.
J Nat Med ; 73(4): 805-813, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31055728

RESUMO

Jatropha multifida is a medicinal plant that belongs to the Euphorbiaceae family. Our investigation revealed that the chloroform extract of J. multifida stems showed anti-melanin deposition activity against α-melanocyte stimulating hormone (α-MSH)- and 3-isobutyl-1-methylxanthine (IBMX)-induced melanogenesis in the mouse melanoma cell line (B16-F10). Further fractionation and purification of the major constituents led to the isolation of two coumarins (1 and 2) and seven known lignoids (3-9). All isolated compounds exhibited anti-melanin deposition activities against the mouse melanoma cell line (B16-F10) with IC50 values ranging from 37.5 to 560.1 µM, without any cytotoxicity even at high concentrations, except for 8. Further mechanistic studies suggested that 9 downregulated tyrosinase mRNA expression, while the anti-melanin deposition activities of 4 and 8 appeared to be unrelated to tyrosinase inhibition and the downregulated expression of the key melanogenesis-associated mRNAs. These results suggested that J. multifida could possess potent skin whitening ingredients.


Assuntos
Jatropha/química , Melaninas/metabolismo , Melanoma Experimental/tratamento farmacológico , Extratos Vegetais/farmacologia , alfa-MSH/farmacologia , Animais , Linhagem Celular Tumoral , Regulação para Baixo/efeitos dos fármacos , Camundongos , Monofenol Mono-Oxigenase/metabolismo
7.
Fitoterapia ; 134: 101-107, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30794917

RESUMO

Four new bis-iridoid glycosides, saungmaygaosides A-D (1-4), and six known iridoid glycosides (5-10) were isolated from the n-butanol extract of the stems of Picrorhiza kurroa collected in Myanmar. Their structures were elucidated by extensive spectroscopic techniques. All of the isolates were assayed for anti-Vpr activity, using TREx-HeLa-Vpr cells. Among the isolates, saungmaygaoside D (4), sylvestroside IV dimethyl acetal (7), and sweroside (8) were the most potent inhibitors with effective doses of 5 and 10 µM, respectively, without showing any notable cytotoxicities.


Assuntos
Antivirais/farmacologia , Produtos do Gene vpr/antagonistas & inibidores , Glicosídeos Iridoides/farmacologia , Picrorhiza/química , Antivirais/isolamento & purificação , Células HeLa , Humanos , Glicosídeos Iridoides/isolamento & purificação , Estrutura Molecular , Mianmar , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Caules de Planta/química
8.
J Nat Med ; 73(3): 589-596, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30790130

RESUMO

Picrasma javanica Blume (Simaroubaceae) is a medium-sized tree that is distributed widely in tropical Asia. In our previous study, we isolated quassinoids from P. javanica bark collected in Myanmar, and reported their antiproliferative activities. In our ongoing research for the discovery of bioactive compounds from Myanmar medicinal plants, two new quassinoids, (16R)-methoxyjavanicin B (1) and (16S)-methoxyjavanicin B (2), along with seven known compounds (3-9), were isolated during the phytochemical investigation of the CHCl3 soluble portion of the MeOH extract of P. javanica wood. The structures of the new compounds were elucidated by analyses of their spectroscopic data (1D- and 2D-NMR, HRESIMS, and CD). A cytotoxicity assay revealed that compound 8 showed moderate activities against all tested cancer cell lines, the human lung (A549), breast (MCF7), and cervical (HeLa), and the normal fibroblast cell line, with IC50 values ranging from 48.6 to 65.9 µM. Furthermore, the antibacterial assay demonstrated that 1 and 2 had the highest activities (MIC value of 1.6 µM each), followed by 5 and 3 (MIC values of 3.1 and 6.3 µM, respectively) against the Gram-positive bacterium B. subtilis.


Assuntos
Antibacterianos/farmacologia , Bacillus subtilis/crescimento & desenvolvimento , Proliferação de Células/efeitos dos fármacos , Neoplasias/tratamento farmacológico , Picrasma/química , Quassinas/farmacologia , Células A549 , Linhagem Celular Tumoral , Células HeLa , Humanos , Células MCF-7 , Testes de Sensibilidade Microbiana , Estrutura Molecular , Mianmar , Extratos Vegetais/farmacologia , Plantas Medicinais/química , Madeira/química
9.
Chem Biodivers ; 16(4): e1800657, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30645035

RESUMO

A new dinorcassane diterpenoid, seikphoochinal A (1), and four known compounds, pinostrobin (2), 4',7-dimethylkaempferol (3), and galanals A (4) and B (5), were isolated from the chloroform-soluble crude extract of wild type Boesenbergia rotunda rhizomes collected in Lower Myanmar. The chemical structures of these compounds were identified, using a combination of spectroscopic methods. The presence of the diterpenoids 1, 4, and 5 demonstrated the structural diversity of wild type B. rotunda. Among the isolates, compounds 4 and 5 exhibited significant antiproliferative activities against a small panel of human cancer cell lines, including lung (LK-2, A549), stomach (ECC4), breast (MCF7), cervix (HeLa), and prostate (DU145).


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Diterpenos/farmacologia , Extratos Vegetais/farmacologia , Zingiberaceae/química , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Diterpenos/química , Diterpenos/isolamento & purificação , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Estrutura Molecular , Mianmar , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Relação Estrutura-Atividade
10.
Fitoterapia ; 133: 35-42, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30572089

RESUMO

Three new lignoids, premnan A (1), premnan B (2), and tauntangyiol C (3), were isolated from Premna serratifolia wood, a traditional cosmetic plant in Myanmar, together with a new lignoid, premnan C (4) assumed to be an artifact, one natural new lignoid (5), and three known lignoids (6-8). The structures of the new compounds 1-4 were elucidated based on 1D and 2D NMR, IR spectroscopy, and HRESIMS. The absolute configurations of 1-4 were also determined by optical rotation, circular dichroism (CD) data analyses, and comparisons with the reported literature. All isolated compounds were tested for their melanogenesis activities against the B16-F10 mouse melanoma cell line. Compounds 1 and 4 showed melanogenesis enhancing activities of 31% and 50%, respectively, at a 50 µM concentration. Compounds 2, 3, and 6 increased melanin production by 67%, 30%, and 45%, respectively, at a 100 µM concentration, without any cytotoxicity.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Lamiaceae/química , Lignanas/farmacologia , Madeira/química , Animais , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Lignanas/isolamento & purificação , Melaninas , Melanoma Experimental , Camundongos , Estrutura Molecular , Mianmar , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia
11.
J Nat Med ; 72(3): 803-807, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29569222

RESUMO

Marine organisms such as marine sponges and soft corals are valuable sources of pharmacologically active secondary metabolites. In our ongoing research on the discovery of new secondary metabolites from marine organisms, two new pyrrolo-2-aminoimidazoles, clathriroles A (1) and B (2), were isolated from the water-soluble portion prepared from the methanol and acetone (2:1) extract of the marine sponge, Clathria prolifera, collected in Myanmar. The chemical structures of the isolated compounds were determined using extensive spectroscopic techniques, including NMR, HRESIMS, IR, and optical rotation, and comparisons with the reported literature. The spectroscopic analyses of 1 and 2 suggested that 1 is an enantiomer of antifungal N-methylmanzacidin C isolated from the marine sponge Axinella brevistyla, whereas 2 is a diastereomer of manzacidin D at C-11 isolated from the marine sponge Astrosclera willeyana. To the best of our knowledge, this is the first report of the isolation of the pyrrolo-2-aminoimidazole compounds from C. prolifera. Furthermore, in contrast to the potency of N-methylmanzacidin C against Saccharomyces cerevisiae, the antifungal assay revealed that 1 and 2 lack any activity against this strain. Thus, these observations may suggest that the absolute configurations at both C-9 and C-11 play an important role in controlling the antifungal activity of this type of compound.


Assuntos
Imidazóis/química , Poríferos/química , Animais , Estrutura Molecular
12.
Fitoterapia ; 127: 308-313, 2018 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-29540314

RESUMO

Two new tetrahydrofuran lignans, taungtangyiols A (1) and B (2), and eight known furofuran lignans (3-10), were isolated from the chloroform extract of Premna integrifolia wood collected in Myanmar. Their structures were elucidated by extensive spectroscopic techniques. The X-ray crystal structure of 1 clearly indicated its relative configuration. Taungtangyiols A (1) and B (2) inhibited the deposition of melanin in B16F10 mouse melanoma cells, with IC50 values of 50.7 and 40.9 µM, respectively, without notable cytotoxicity. An SAR study demonstrated that the furofuran and dioxymethylene moieties of the lignans play a vital role in inhibiting melanogenesis.


Assuntos
Furanos/isolamento & purificação , Lamiaceae/química , Lignanas/isolamento & purificação , Melaninas/antagonistas & inibidores , Madeira/química , Animais , Linhagem Celular Tumoral , Melanoma Experimental , Camundongos , Estrutura Molecular , Mianmar
14.
Fitoterapia ; 122: 34-39, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28827004

RESUMO

Four new labdane diterpenoids, 12ß-hydroxy-15-norlabda-8(17),13(14)-dien-16-oic acid (1), (E)-15-ethoxy-15-methoxylabda-8(17),12-dien-16-al (2), (E)-15α-ethoxy-14α-hydroxylabda-8(17),12-dien-16-olide (3), and 15-ethoxy-12ß-hydroxylabda-8(17),13(14)-dien-16,15-olide (4) were isolated from the methanol extract of Curcuma amada rhizomes collected in Myanmar, together with 13 known analogs. Their structures were elucidated by extensive spectroscopic techniques. All of the isolates were evaluated for their antiproliferative activities against a small panel of five different human cancer cell lines (A549, human lung cancer; HeLa, human cervical cancer; MCF7, human breast cancer; PANC-1 and PSN-1, human pancreatic cancer). Among the isolates, compounds 2-4, 7, 8, 12, and 17 showed mild antiproliferative activities with IC50 values ranging from 19.7 to 96.1µM. (E)-14-Hydroxy-15-norlabda-8(17),12-dien-16-al (11) exhibited strong antiproliferative activities selectively against HeLa, PANC-1, and PSN-1 cells, with IC50 values of 5.88, 1.00, and 3.98µM, respectively. These potencies were comparable to those of the positive control, 5-fluorouracil.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Curcuma/química , Diterpenos/farmacologia , Antineoplásicos Fitogênicos/isolamento & purificação , Linhagem Celular Tumoral , Diterpenos/isolamento & purificação , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Estrutura Molecular , Mianmar , Plantas Medicinais/química , Rizoma/química
15.
J Nat Med ; 71(4): 579-589, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-28681118

RESUMO

Human immunodeficiency virus type-1 (HIV-1) is a lentiviral family member that encodes the retroviral Gag, Pol, and Env proteins, along with six additional accessory proteins, Tat, Rev, Vpu, Vif, Nef, and Vpr. The currently approved anti-HIV drugs target the Pol and Env encoded proteins. However, these drugs are only effective in reducing viral replication. Furthermore, the drugs' toxicities and the emergence of drug-resistant strains have become serious worldwide problems. Resistance eventually arises to all of the approved anti-HIV drugs, including the newly approved drugs that target HIV integrase (IN). Drug resistance likely emerges because of spontaneous mutations that occur during viral replication. Therefore, new drugs that effectively block other viral components must be developed to reduce the rate of resistance and suppress viral replication with little or no long-term toxicity. The accessory proteins may expand treatment options. Viral protein R (Vpr) is one of the promising drug targets among the HIV accessory proteins. However, the search for inhibitors continues in anti-HIV drug discovery. In this review, we summarize the naturally occurring compounds discovered from two Myanmar medicinal plants as well as their structure-activity relationships. A total of 49 secondary metabolites were isolated from Kaempferia pulchra rhizomes and Picrasama javanica bark, and the types of compounds were identified as isopimarane diterpenoids and picrasane quassinoids, respectively. Among the isolates, 7 diterpenoids and 15 quassinoids were found to be Vpr inhibitors lacking detectable toxicity, and their potencies varied according to their respective functionalities.


Assuntos
Fármacos Anti-HIV/farmacologia , Produtos do Gene vpr/antagonistas & inibidores , HIV-1/efeitos dos fármacos , Picrasma/química , Extratos Vegetais/farmacologia , Zingiberaceae/química , Produtos do Gene vpr do Vírus da Imunodeficiência Humana/antagonistas & inibidores , Diterpenos/farmacologia , HIV-1/fisiologia , Humanos , Mianmar , Fitoterapia , Plantas Medicinais , Quassinas/farmacologia , Relação Estrutura-Atividade , Replicação Viral/efeitos dos fármacos
16.
BMC Complement Altern Med ; 17(1): 96, 2017 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-28173854

RESUMO

BACKGROUND: To contribute to the development of novel anti-influenza drugs, we investigated the anti-influenza activity of crude extracts from 118 medicinal plants collected in Myanmar. We discovered that extract from the stems of Jatropha multifida Linn. showed anti-influenza activity. J. multifida has been used in traditional medicine for the treatment of various diseases, and the stem has been reported to possess antimicrobial, antimalarial, and antitumor activities. However, the anti-influenza activity of this extract has not yet been investigated. METHODS: We prepared water (H2O), ethyl acetate (EtOAc), n-hexane (Hex), and chloroform (CHCl3) extracts from the stems of J. multifida collected in Myanmar, and examined the survival of Madin-Darby canine kidney (MDCK) cells infected with the influenza A (H1N1) virus, and the inhibitory effects of these crude extracts on influenza A viral infection and growth in MDCK cells. RESULTS: The H2O extracts from the stems of J. multifida promoted the survival of MDCK cells infected with the influenza A H1N1 virus. The EtOAc and CHCl3 extracts resulted in similar, but weaker, effects. The H2O, EtOAc, and CHCl3 extracts from the stems of J. multifida inhibited influenza A virus H1N1 infection; the H2O extract possessed the strongest inhibitory effect on influenza infection in MDCK cells. The EtOAc, Hex, and CHCl3 extracts all inhibited the growth of influenza A H1N1 virus, and the CHCl3 extract demonstrated the strongest activity in MDCK cells. CONCLUSION: The H2O or CHCl3 extracts from the stems of J. multifida collected in Myanmar demonstrated the strongest inhibition of influenza A H1N1 viral infection or growth in MDCK cells, respectively. These results indicated that the stems of J. multifida could be regarded as an anti-influenza herbal medicine as well as a potential crude drug source for the development of anti-influenza compounds.


Assuntos
Antivirais/uso terapêutico , Vírus da Influenza A Subtipo H1N1/efeitos dos fármacos , Influenza Humana/tratamento farmacológico , Fitoterapia , Extratos Vegetais/uso terapêutico , Animais , Antivirais/farmacologia , Linhagem Celular , Cães , Humanos , Influenza Humana/virologia , Células Madin Darby de Rim Canino , Medicina Tradicional , Mianmar , Extratos Vegetais/farmacologia , Caules de Planta
17.
Bioorg Med Chem Lett ; 26(7): 1789-93, 2016 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-26916438

RESUMO

Viral protein R (Vpr), an accessory gene of HIV-1, plays important roles in viral pathogenesis. Screening of Myanmar medicinal plants that are popular as primary treatments for HIV/AIDS and for HIV-related problems revealed the potent anti-Vpr activity of the CHCl3-soluble extract of Kaempferia pulchra rhizomes, in comparison with that of the positive control, damnacanthal. Fractionation of the active CHCl3-soluble extract led to the identification of 30 isopimarane diterpenoids, including kaempulchraols A-W (1-23). All isolates were assayed for anti-Vpr activity against TREx-HeLa-Vpr cells, in which Vpr expression is tightly regulated by tetracycline. Kaempulchraols B (2), D (4), G (7), Q (17), T (20), U (21), and W (23) exhibited potent anti-Vpr activity, at concentrations ranging from 1.56 to 6.25µM. The structure-activity relationships of the active kaempulchraols suggested that the presence of a hydroxy group at C-14 in an isopimara-8(9),15-diene skeleton and the presence of an acetoxy group at C-1 or C-7 in an isopimara-8(14),15-diene skeleton are the critical factors for the inhibitory effects against TREx-HeLa-Vpr cells.


Assuntos
Fármacos Anti-HIV/química , Fármacos Anti-HIV/farmacologia , Diterpenos/química , Diterpenos/farmacologia , Produtos do Gene vpr/antagonistas & inibidores , HIV-1/efeitos dos fármacos , Zingiberaceae/química , Fármacos Anti-HIV/isolamento & purificação , Diterpenos/isolamento & purificação , Produtos do Gene vpr/metabolismo , Infecções por HIV/tratamento farmacológico , Infecções por HIV/virologia , HIV-1/metabolismo , Células HeLa , Humanos , Rizoma/química , Relação Estrutura-Atividade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA