Effectiveness of plant-based hand sanitizer incorporating Quercus infectoria gall extract.

AIMS: Quercus infectoria (Qi), a traditional herbal plant with a broad spectrum of activities on multidrug-resistant bacteria, has been developed for hand sanitizer applications. METHODS AND RESULTS: Antimicrobial activity was evaluated using agar-well diffusion and broth microdilution method. Bactericidal activity was determined following the European Standard 1276 antibacterial suspension test. Neutralization assay was performed to assess antirespiratory syncytial virus. Safety, stability, and skin permeation of Qi hand gel was investigated. Qi hand sanitizer gel inhibited microorganisms ranging from 99.9% to 99.999% against Enterococcus faecalis, Staphylococcus aureus, methicillin-resistant Staph. aureus, Staph. epidermidis, Staph. pseudintermedius, Staph. saprophyticus, Streptococcus pyogenes, Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans. A significant reduction in main human dermatophytes including Microsporum canis, M. gypseum, and Talaromyces marneffei of ∼50% was observed (P < .05). Qi hand sanitizer gel inactivated >99% viral particles entering human laryngeal epidermoid carcinoma cells in a dose-dependent manner. Scanning electron micrographs further illustrated that Qi hand sanitizer gel disrupted microbial cell membrane after 1-min contact time resulting in cell death. Qi hand sanitizer gel delivered emollient compounds through simulated human skin layers and showed no cytotoxicity on fibroblast cells. Moreover, Qi hand sanitizer gel demonstrated stability under extreme conditions. CONCLUSIONS: Qi hand sanitizer gel was able to inhibit various microorganisms including bacteria, dermatophytes, and virus.

Synergistic effects of polymyxin and vancomycin combinations on carbapenem- and polymyxin-resistant Klebsiella pneumoniae and their molecular characteristics.

IMPORTANCE: This study provides insights into the mechanisms of polymyxin resistance in K. pneumoniae clinical isolates and demonstrates potential strategies of polymyxin and vancomycin combinations for combating this resistance. We also identified possible mechanisms that might be associated with the treatment of these combinations against carbapenem- and polymyxin-resistant K. pneumoniae clinical isolates. The findings have significant implications for the development of alternative therapies and the effective management of infections caused by these pathogens.

Facile deposition of biogenic silver nanoparticles on porous alumina discs, an efficient antimicrobial, antibiofilm, and antifouling strategy for functional contact surfaces.

Surface modification is an emerging strategy for the design of contact materials. Fabricated alumina discs were functionalized by deposition of biogenic silver nanoparticles. The surfaces were characterized for physico-chemical, antibacterial and antibiofilm properties against microbial pathogens. The surface demonstrated improved hydrophobicity and a surface silver nanoparticle content of 6.4 w%. A reduction of more than 99.9% in CFU mL-i was observed against the Gram-positive and Gram-negative bacteria tested, with >90% reduction of the fungal isolate. After 4 h, microbial adhesion was reduced by >99.9 and 90% for Escherichia coli and Staphylococcus aureus, respectively. Scanning electron micrographs further revealed a biofilm reduction. Cell viability tests indicated a bioincompatibility higher than 80% with Caco-2 and HaCaT cell lines after 48 h contact. The results suggest that deposition of biogenic silver nanoparticles on the surface of contact materials could be employed as a strategy to prevent biofilm formation.

Bioactive fractions of Eucalyptus camaldulensis inhibit important foodborne pathogens, reduce listeriolysin O-induced haemolysis, and ameliorate hydrogen peroxide-induced oxidative stress on human embryonic colon cells.

Extract of E. camaldulensis was partitioned into aqueous and ethanol fractions by a precipitation and sedimentation-based technique and profiled for phytochemical components. Antimicrobial evaluation yielded inhibitory concentrations of 16-64 and 158-316 µg/mL, and bactericidal concentrations of 32-64 and 316->2528 µg/mL for ethanol and aqueous fractions, respectively. Antioxidant activities evaluated using 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic) acid assays showed IC50 values of 7.07 and 65.67 µg/mL, and 17.96 and 201.3 µg/mL for aqueous and ethanol fractions. Total phenolic content of 9.04 ± 0.26 and 3.58 ± 0.04 GAE/mg fraction, and flavonoid content of 2.07 ± 0.02 and 3.37 ± 0.05 QE/mg fraction were recorded for aqueous and ethanol fractions. At subinhibitory concentrations fractions significantly reduced listeriolysin O-induced haemolysis (p < 0.05), and ameliorated H2O2-induced toxicity by 8-23 and 15-83%. Nitrite production reduced by 4-17 and 3-14 µM following fractions treatment. The fractions showed bioactive properties, with oxidative stress amelioratory effects, and could be a potentials source of preservatives and functional food additives.

Antibacterial-coated silk surgical sutures by ex situ deposition of silver nanoparticles synthesized with Eucalyptus camaldulensis eradicates infections.

Surgical site infection arising from microbial contamination of surgical wounds is a major cause of surgical complications and prolong hospital stay. In this study, silver nanoparticles (AgNPs) biosynthesized using Eucalyptus camaldulensis extract were deposited on silk surgical sutures by ex situ method. Adherence of AgNPs to the surface of sutures was observed, with significantly reduced surface roughness (323.7 ± 16.64 nm), compared with uncoated sutures (469.3 ± 7.31 nm) (P < .001). Elasticity of AgNPs-coated (13 ± 1.485%) and uncoated (8 ± 0.728%) sutures was also significantly different (P < .05). Quantification of AgNPs demonstrated release of 3.88, 5.33, 5.44, 6.14% on day 1, 3, 5, 7, respectively from total Ag+ concentration (6.14 ± 0.14 µg/mL). The coated sutures produced a strong bacteriostatic effect on Staphylococcus aureus, an important wound pathogen with approximately 99% reduction in growth. In contrast, bactericidal effects were observed with Gram-negative pathogens including Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Cytocompatibility tested on human keratinocyte cells exhibited approximately 80% cell viability. The coated sutures revealed stable antibacterial properties up to 12 weeks. This work suggested the potency of AgNPs-coated sutures as a suitable biocompatible medical device for the management of surgical site infections.

Antioxidant Properties and Antibacterial Effects of Eucalyptus camaldulensis Ethanolic Leaf Extract on Biofilm Formation, Motility, Hemolysin Production, and Cell Membrane of the Foodborne Pathogen Listeria monocytogenes.

Consumer concerns toward chemical preservatives have resulted in increased search for healthy green alternative. In this study, the antioxidant activity and antibacterial effects of Eucalyptus camaldulensis ethanolic leaf extract against Listeria monocytogenes, a serious foodborne pathogen, was evaluated. Total phenolic and flavonoid contents of the extract were 11.10 mg garlic acid equivalent/mg extract and 15.05 mg quercetin equivalent/mg extract, respectively. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration of the extract was 64-128 µg/mL and 256-512 µg/mL, respectively. Time-kill assay revealed growth inhibitory effects after 4-h treatment of the bacteria with the extract. A reduction of ≈2-3 log colony-forming units per milliliter was observed against the tested food and environmental isolates after challenging the pathogens with the extract at MIC for 6 h. Sub-MICs of the extract significantly inhibited motility and listeriolysin O production up to 80%, with 60% inhibition of biofilm formation (p < 0.05). Antioxidant assay revealed free radical scavenging activity with 50% inhibitory concentration (IC50) of 57.07 µg/mL for 2,2-diphenyl-1-picrylhydrazyl and 29.01 µg/mL for ABTS [2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] assay. Ferric reducing antioxidant power assay further showed a total antioxidant power equivalent to 92.93 µM ascorbic acid equivalent/mg extract. As the extract exhibited profound antilisterial activity and good radical scavenging ability, it might serve as a potential alternative source of biopreservative agent against L. monocytogenes.