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1.
Br J Nutr ; 118(9): 661-672, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29185927

RESUMO

Our previous study demonstrated that supplemental psyllium fibre increased cytoprotective heat-shock protein (Hsp) 25 levels in the intestinal cells of mice. Here, we examined the effect of psyllium fibre on colonic gene and protein expression and faecal microbiota in normal and colitic mice to improve the understanding of the preventive role of the supplement. DNA microarray analysis revealed that a 10 % psyllium fibre diet administered for 5 d up-regulated eleven extracellular matrix (ECM)-associated genes, including collagens and fibronectins, in normal mice. Acute colitis was induced using dextran sodium sulphate (DSS) in mice that were administered a pre-feeding 5 to 10 % psyllium fibre diet for 5 d. Psyllium fibre partially ameliorated or resolved the DSS-induced colon damage and inflammation characterised by body weight loss, colon shortening, increased levels of pro-inflammatory cytokines and decreased tight junction protein expression in the colon. Analysis of faecal microbiota using denaturing gradient gel electrophoresis of the PCR-amplified 16S rRNA gene demonstrated that psyllium fibre affected the colonic microbiota. Intestinal permeability was evaluated by growing intestinal Caco-2 cell monolayers on membrane filter supports coated with or without fibronectin and collagen. Cells grown on collagen and fibronectin coating showed higher transepithelial electrical resistance, indicating a strengthening of barrier integrity. Therefore, increased Hsp25 levels and modification of colonic ECM contribute to the observed psyllium-mediated protection against DSS-induced colitis. Furthermore, ECM modification appears to play a role in the strengthening of the colon barrier. In conclusion, psyllium fibre may be useful in the prevention of intestinal inflammatory diseases.


Assuntos
Colite/tratamento farmacológico , Fibras na Dieta/farmacologia , Inflamação/tratamento farmacológico , Intestinos/efeitos dos fármacos , Psyllium/farmacologia , Animais , Células CACO-2 , Colite/induzido quimicamente , Colágeno/farmacologia , Citocinas/sangue , DNA Bacteriano/isolamento & purificação , Sulfato de Dextrana , Matriz Extracelular/genética , Matriz Extracelular/metabolismo , Fezes/microbiologia , Fibronectinas/farmacologia , Microbioma Gastrointestinal/efeitos dos fármacos , Humanos , Absorção Intestinal/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Endogâmicos ICR , Permeabilidade/efeitos dos fármacos , RNA Ribossômico 16S/isolamento & purificação , Junções Íntimas/genética , Junções Íntimas/metabolismo
2.
Int J Food Sci Nutr ; 68(8): 941-951, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28438083

RESUMO

The present study investigated the antiallergic and anti-inflammatory effects of 10-hydroxy-cis-12-octadecenoic acid (HYA), a novel gut microbial metabolite of linoleic acid, in NC/Nga mice, a model of atopic dermatitis (AD). Feeding HYA decreased the plasma immunoglobulin E level and skin infiltration of mast cells with a concomitant decrease in dermatitis score. HYA feeding decreased TNF-α and increased claudin-1, a tight junction protein, levels in the mouse skin. Cytokine expression levels in the skin and intestinal Peyer's patches cells suggested that HYA improved the Th1/Th2 balance in mice. Immunoglobulin A concentration in the feces of the HYA-fed mice was approximately four times higher than that in the control mice. Finally, denaturing gradient gel electrophoresis of the PCR-amplified 16 S rRNA gene of fecal microbes indicated the modification of microbiota by HYA. Taken together, the alterations in the intestinal microbiota might be, at least in part, associated with the antiallergic effect of HYA.


Assuntos
Dermatite Atópica/dietoterapia , Suplementos Nutricionais , Microbioma Gastrointestinal/efeitos dos fármacos , Microbioma Gastrointestinal/fisiologia , Ácido Linoleico/farmacologia , Ácidos Oleicos/farmacologia , Ração Animal , Animais , Comportamento Animal/efeitos dos fármacos , Citocinas/genética , Citocinas/metabolismo , Dieta/veterinária , Fezes/química , Regulação da Expressão Gênica/fisiologia , Imunoglobulina A/química , Inflamação/tratamento farmacológico , Ácido Linoleico/administração & dosagem , Ácido Linoleico/química , Camundongos , Estrutura Molecular , Ácidos Oleicos/administração & dosagem , Ácidos Oleicos/química , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa
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