RESUMO
An arabinogalactan-protein (WSSP-AGP) was isolated from the tuberous cortex of the white-skinned sweet potato (WSSP; Ipomoea batatas L.). It consists of 95% (w/w) carbohydrate and 5% (w/w) protein with high contents of hydroxyproline, alanine, and serine. Its sugar composition is α-L-Rha:α-L-Ara:ß-D-Gal:ß-D-GlcA in a molar ratio of 1.0:4.1:7.6:1.3. Its weight-average molecular weight was estimated to be 126,800 g/mol by high-performance size exclusion chromatography coupled with multiangle laser light scattering. Structural analysis indicated that WSSP-AGP is a (1â3)-ß-D-galactan highly branched at O-6 with (1â6)-ß-D-galactan, in which the branched chains are substituted at the O-3 position with α-L-Araf-(1â and α-L-Araf-(1â5)-α-L-Araf-(1â and at the O-6 position typically with α-L-Rhap-(1â4)-ß-D-GlcAp-(1â as terminating groups. Continuous administration of WSSP-AGP to KKAy mice significantly lowered fasting plasma glucose levels. This indicates that WSSP-AGP plays an important role in the hypoglycemic effects of WSSP.