RESUMO
Heparinized cadaveric organ donors are a potential source of hematopoietic cells for transplantation purposes. The aim of this study was to optimize the harvest of early hematopoietic cells from cadaver donors. Accordingly, we noticed that bone marrow cells harvested from cadaver donors should be resuspended in RPMI or Iscove's medium supplemented with heparin or ACD. Bags with harvested marrow should contain 20% atmosphere air during short-term storage/transportation. Finally, we noticed that BMMNC survive short-term storage better if the collected marrow is not depleted of erythrocytes.
Assuntos
Células da Medula Óssea/citologia , Cadáver , Células-Tronco Hematopoéticas/citologia , Doadores de Tecidos , Adulto , Anticoagulantes/farmacologia , Medula Óssea , Morte Encefálica , Causas de Morte , Divisão Celular/efeitos dos fármacos , Separação Celular/métodos , Meios de Cultura , Ácido Edético/farmacologia , Feminino , Células-Tronco Hematopoéticas/efeitos dos fármacos , Heparina/farmacologia , Humanos , Masculino , Coleta de Tecidos e Órgãos/métodosRESUMO
The effect of the genomic and subgenomic leader sequence of potato leafroll polerovirus on the efficiency of translation of the downstream located genes has been studied. The results obtained in vitro and in vivo indicate that neither leader sequence functions as translational enhancer, a generally important feature of leader sequences. Deletion analyses demonstrated that both leader sequences not only decrease translation of the downstream located genes but also alter the ratio of the synthesized proteins. A correlation between the in vitro and in vivo results can be established in the case of the subgenomic leader sequence.