RESUMO
Research for innovative drugs is crucial to contribute to parasitic infections control and eradication. Inspired by natural antiprotozoal triterpenes, a library of 12 hemisynthetic 3-O-arylalkyl esters was derived from ursolic and oleanolic acids through one-step synthesis. Compounds were tested on Trypanosoma, Leishmania and the WI38 cell line alongside with a set of triterpenic acids. Results showed that the triterpenic C3 esterification keeps the antitrypanosomal activity (IC50 ≈1.6-5.5â µm) while reducing the cytotoxicity compared to parent acids. Unsaturation of the ester alkyl chain leads to an activity loss interestingly kept when a sterically hindered group replaces the double bond or shields the ester group. An ursane/oleanane C3 hydroxylation was the only important feature for antileishmanial activity. Two candidates, dihydrocinnamoyl and 2-fluorophenylpropionyl ursolic acids, were tested on an acute mouse model of African trypanosomiasis with significant parasitemia reduction at day 5 post-infection for the dihydrocinnamoyl derivative. Further evaluation on other alkyl/protective groups should be investigated both inâ vitro and inâ vivo.
Assuntos
Ésteres/farmacologia , Triterpenos/farmacologia , Tripanossomicidas/farmacologia , Animais , Desenho de Fármacos , Avaliação Pré-Clínica de Medicamentos , Ésteres/síntese química , Ésteres/toxicidade , Feminino , Leishmania mexicana/efeitos dos fármacos , Camundongos , Testes de Sensibilidade Parasitária , Triterpenos/síntese química , Triterpenos/toxicidade , Tripanossomicidas/síntese química , Tripanossomicidas/toxicidade , Trypanosoma brucei brucei/efeitos dos fármacosRESUMO
Plants in the Amaryllidaceae family synthesize a diversity of bioactive alkaloids. Some of these plant species are not abundant and have a low natural multiplication rate. The aims of this work were the alkaloids analysis of a Habranthus cardenasianus bulbs extract, the evaluation of its inhibitory activity against cholinesterases, and to test several propagation strategies for biomass production. Eleven compounds were characterized by GC-MS in the alkaloid extract, which showed a relatively high proportion of tazettine. The known alkaloids tazettine, haemanthamine, and the epimer mixture haemanthidine/6-epi-haemanthidine were isolated and identified by spectroscopic methods. Inhibitory cholinesterases activity was not detected. Three forms of propagation were performed: bulb propagation from seed, cut-induced bulb division, and micropropagated bulbs. Finally, different imbibition and post-collection times were evaluated in seed germination assays. The best propagation method was cut-induced bulb division with longitudinal cuts into quarters (T1) while the best conditions for seed germination were 0-day of post-collection and two days of imbibition. The alkaloids analyses of the H. cardenasianus bulbs showed that they are a source of anti-tumoral alkaloids, especially pretazettine (tazettine) and T1 is a sustainable strategy for its propagation and domestication to produce bioactive alkaloids.
Assuntos
Alcaloides/análise , Alcaloides/farmacologia , Amaryllidaceae/química , Amaryllidaceae/crescimento & desenvolvimento , Inibidores da Colinesterase/farmacologia , Alcaloides/química , Alcaloides/isolamento & purificação , Alcaloides de Amaryllidaceae/análise , Biomassa , Butirilcolinesterase/metabolismo , Inibidores da Colinesterase/química , Cromatografia Gasosa-Espectrometria de Massas , Germinação , Estrutura Molecular , Fenantridinas/análise , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Raízes de Plantas/crescimento & desenvolvimento , Sementes/crescimento & desenvolvimento , Fatores de TempoRESUMO
Introducción: Chrysobalanus icaco L. (Chrysobalanaceae) es un arbusto utilizado en la fitoterapia tradicional latinoamericana, pero en la provincia de Chiriquí se ha registrado el uso de su fruto para hacer conservas, no de forma medicinal. Objetivo: Caracterizar los metabolitos secundarios presentes en las hojas y semillas de Chrysobalanus icaco y evaluar su actividad biológica in vitro. Métodos: Los metabolitos secundarios presentes en los extractos de las hojas, semilla inmadura y madura de Chrysobalanus icaco, se detectaron a través de tamizaje fitoquímico y purificación por técnicas cromatográficas. Posteriormente se evaluó el contenido de polifenoles totales y la actividad antioxidante total de la infusión de las hojas, semillas y pulpa de esta especie, así como la actividad antibacteriana in vitro. Resultados: En las hojas se identificaron por tamizaje fitoquímico, flavonoides, glucósidos cardiotónicos, triterpenos y esteroides. Para la semilla inmadura se encontró presencia mayoritaria de mezclas de azucares, mientras que para la semilla madura se detectaron ácidos grasos en el endocarpio (almendra). Los datos de polifenoles totales en extractos de Icaco mostraron alto contenido de estos metabolitos en la infusión de hojas secas, y la inhibición del radical DPPH fue mayor también para las hojas secas de icaco, seguido de la infusión de semilla inmadura. Por otro lado, la infusión de hojas secas y el licuado de la pulpa fueron los que mostraron una mayor inhibición contra la cepa de Staphylococcus spp. en el ensayo de actividad antibacteriana. Conclusión: Las hojas secas de Chrysobalanus icaco mostraron alto contenido de polifenoles, asociado a su potencial actividad antioxidante y antibacteriana. Además, se aportaron nuevos datos de composición química de la semilla, se observó que los azúcares detectados inicialmente en la semilla inmadura se pierden considerablemente con la maduración del fruto.
Introduction: Chrysobalanus icaco L. (Chrysobalanaceae) is a shrub used in Latin American traditional phytotherapy, but in the province of Chiriquí its fruit has been reported to be used in food preserves, not for medicinal purposes. Objective: Characterize the secondary metabolites present in Chrysobalanus icaco leaves and seeds and evaluate their in vitro biological activity. Methods: The secondary metabolites present in extracts from leaves and immature and mature seeds of icaco were detected by phytochemical screening and purification with chromatographic techniques. Evaluation was then conducted of total polyphenolic content and total antioxidant activity of the leaf, seed and pulp infusion as well as its antibacterial activity in vitro. Results: Phytochemical screening of the leaves found flavonoids, cardiotonic glycosides, triterpenes and steroids. Sugar mixtures were the most abundant components in immature seeds, whereas fatty acids were found in the endocarp (almond) of mature seeds. Total polyphenolic data about icaco extracts showed high contents of these metabolites in the dry leaf infusion. DPPH radical inhibition was also greater for icaco dry leaves, followed by immature leaf infusion. The dry leaf infusion and the pulp shake displayed the greatest inhibition against the Staphylococcus spp. strain in the antibacterial activity test. Conclusion: Icaco dry leaves exhibited a high polyphenolic content, associated to their antioxidant and antibacterial activity. Fresh data were also contributed about the chemical composition of the seed. It was observed that the sugars initially detected in immature seeds are considerably lost as the fruit ripens.
Assuntos
Chrysobalanaceae , Compostos Fitoquímicos , Antibacterianos , Antioxidantes , Técnicas In Vitro , Medicina TradicionalRESUMO
In the present study, a series of new esters of secochiliolide acid (SA), a diterpene isolated from Nardophyllum bryoides, were synthesized in good yield. All compounds were evaluated for their in vitro antiparasitic properties (on Plasmodium falciparum and Trypanosoma brucei brucei) and cytotoxicity (on WI38, normal mammalian cells). They displayed moderate antitrypanosomal activity with IC50 values between 2.55 and 18.14 µm, with selectivity indices >10, and low antiplasmodial effects with IC50 > 29 µm. The only exception was the n-hexyl ester of SA, which showed a strong and selective antiplasmodial activity (IC50 = 1.99 µm and selectivity index = 117.0). The in vivo antimalarial efficacy of this compound was then assessed according to the 4-day suppressive test of Peters in mice. An intraperitoneal treatment at 50 mg kg-1 day-1 induced a slight parasitaemia reduction by 56% which was statistically significant on day 4 post-infection and an increase in the survival time.
Assuntos
Antimaláricos/química , Antiprotozoários/química , Diterpenos/química , Ésteres/química , Propionatos/química , Antimaláricos/isolamento & purificação , Antimaláricos/farmacologia , Antiprotozoários/isolamento & purificação , Antiprotozoários/farmacologia , Asteraceae/química , Asteraceae/metabolismo , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Diterpenos/isolamento & purificação , Diterpenos/farmacologia , Humanos , Extratos Vegetais/química , Plasmodium falciparum/efeitos dos fármacos , Propionatos/isolamento & purificação , Propionatos/farmacologia , Trypanosoma brucei brucei/efeitos dos fármacosRESUMO
Five new neolignans with a bicyclo[2.2.2]octene framework were isolated from an ethanolic extract of the bark of Cordia americana. The structures and relative configurations of the compounds were elucidated by a combination of spectroscopic methods. All the isolated compounds showed good antioxidant activities in the DPPH radical scavenging (0.5-100 µg/mL) and Ferric-reducing antioxidant power (FRAP, 1-100 µg/mL) assays. One of the compounds displayed mild fungistatic activity at 0.1 µmol/spot against Fusarium virguliforme while, at the same time, all compounds were inactive against several strains of Gram (+) and Gram (-) bacteria at all assayed concentrations (10-1,000 µg/mL).
Assuntos
Antifúngicos/isolamento & purificação , Antioxidantes/isolamento & purificação , Cordia/química , Lignanas/isolamento & purificação , Antifúngicos/química , Antifúngicos/farmacologia , Antioxidantes/química , Antioxidantes/farmacologia , Bactérias/efeitos dos fármacos , Compostos Bicíclicos com Pontes , Sequestradores de Radicais Livres/química , Sequestradores de Radicais Livres/isolamento & purificação , Sequestradores de Radicais Livres/farmacologia , Fusarium/efeitos dos fármacos , Lignanas/química , Lignanas/farmacologia , Medicina Tradicional , Estrutura Molecular , Oxirredução , Casca de Planta/química , Extratos Vegetais/química , Extratos Vegetais/isolamento & purificação , Extratos Vegetais/farmacologia , Plantas MedicinaisRESUMO
From the organic extracts of the sponge Siphonochalina fortis, collected at Bahía Bustamante, Chubut, Argentina, three major compounds were isolated and identified as deoxycholic acid 3, 12-diacetate (1), cholic acid 3, 7, 12-triacetate (2) and cholic acid, 3, 7, 12-triacetate. (3). This is the first report of acetylated bile acids in sponges and the first isolation of compound 3 as a natural product. The potential induction of DNA lesions by the isolated compounds was investigated using the comet assay in lymphocytes of human peripheral blood as in vitro model. The results showed that the administration of the bile acid derivatives would not induce DNA damages, indicating that acetylated bile acids are nontoxic metabolites at the tested concentrations. Since the free bile acids were not detected, it is unlikely that the acetylated compounds may be part of the sponge cells detoxification mechanisms. These results may suggest a possible role of acetylated bile acids as a chemical defense mechanism, product of a symbiotic relationship with microorganisms, which would explain their seasonal and geographical variation, and their influence on the previously observed genotoxicity of the organic extract of S. fortis.
Assuntos
Ácidos Cólicos/isolamento & purificação , Dano ao DNA , Mutagênicos/isolamento & purificação , Poríferos/química , Animais , Sobrevivência Celular/efeitos dos fármacos , Células Cultivadas , Ácidos Cólicos/farmacologia , Ensaio Cometa , Avaliação Pré-Clínica de Medicamentos , Humanos , Linfócitos/efeitos dos fármacos , Linfócitos/fisiologia , Mutagênicos/farmacologiaRESUMO
Cucurbitacins are secondary metabolites that mediate insect plant interactions not only as allomones against generalists but also as kairomones for specialist herbivores. This study was undertaken to identify the potential of cucurbitacin derivatives as insect antifeedant agents. The antifeedant capacity against a Cucurbitaceae specialist [ Epilachna paenulata (Coleoptera: Coccinellidae)] and a polyphagous insect [ Pseudaletia adultera (Lepidoptera: Noctuidae)] was evaluated in preference tests in which the insects were given a choice between food plants either treated with the cucurbitacin derivatives or treated with the solvent. The activity was found not to be related to the basic cucurbitacin skeleton, as only 15 of the 28 tested cucurbitacin derivatives were active. Only one of the tested compounds was phagostimulant to the specialist insect (the hemissuccinate of 16-oxo-dihydrocucurbitacin B derivative), while all other active derivatives were deterrent against one of the insects (13 compounds) or both of them (3 compounds). Changes in ring A of the cucurbitacins, as well as in the side chain, modified the activity. As a general trend, when chemical modifications of the basic structure produced a change in activity, the response was opposite in both insects used as biodetectors, indicating that a selective variation in the activity may be achieved by chemical modifications of the cucurbitacin skeleton.
Assuntos
Besouros/fisiologia , Cucurbitaceae/química , Cucurbitacinas/farmacologia , Lepidópteros/efeitos dos fármacos , Lepidópteros/fisiologia , Extratos Vegetais/farmacologia , Animais , Besouros/efeitos dos fármacos , Cucurbitacinas/química , Comportamento Alimentar/efeitos dos fármacos , Estrutura Molecular , Especificidade da EspécieRESUMO
The chemical profile and botanical origin of Andean Argentinian propolis were studied by HPLC-ESI-MS/MS and GC-MS techniques as well as the antifungal activity according to CLSI protocols. Dermatophytes and yeasts tested were strongly inhibited by propolis extracts (MICs between 31.25 and 125 µg/mL). The main antifungal compounds were: 3'methyl-nordihydroguaiaretic acid (MNDGA) 1, nordihydroguaiaretic acid (NDGA) 2 and a NDGA derivative 3, showing strong activity against Trichophyton mentagrophytes, T. rubrum and Microsporum gypseum (MICs between 15.6 and 31.25 µg/mL). The lignans 1 and 2 showed activities against clinical isolates of Candidas spp., Cryptococcus spp., T. rubrum and T. mentagrophytes (MICs and MFCs between 31.25 and 62.5 µg/mL). The lignan and volatile organic compounds (VOCs) profiles from propolis matched with those of exudates of Larrea nitida providing strong evidences on its botanical origin. These results support that Argentinian Andean propolis are a valuable natural product with potential to improve human health. Six compounds (1-6) were isolated from propolis for the first time, while compounds 1 and 3-6 were reported for first time as constituents of L. nitida Cav.
Assuntos
Antifúngicos/farmacologia , Cromatografia Líquida de Alta Pressão/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Larrea/química , Espectrometria de Massas/métodos , Própole/farmacologia , Espectroscopia de Ressonância Magnética , Testes de Sensibilidade MicrobianaRESUMO
Chemical investigation of the roots of Wilbrandia ebracteata Cogn. (Cucurbitaceae) led to the isolation of two new (1- 2) and four known (3- 6) cucurbitacins. Their structures were elucidated by NMR and MS and compared with related compounds. The in vitro cytotoxicity of isolated compounds was evaluated against RD, KB, HCT-8, and A549 cell lines showing strong activity.
Assuntos
Cucurbitaceae/química , Cucurbitacinas/farmacologia , Extratos Vegetais/química , Brasil , Linhagem Celular Tumoral , Sobrevivência Celular , Cromatografia Líquida de Alta Pressão , Cucurbitacinas/química , Cucurbitacinas/isolamento & purificação , Humanos , Medicina Tradicional , Estrutura Molecular , Raízes de Plantas/química , Plantas Medicinais , Espectrometria de Massas por Ionização por ElectrosprayRESUMO
Investigation of the organic extracts of the roots of Maytenus vitis-idaea and Maytenus spinosa, collected in the province of Salta, Argentina, led to isolation of eighteen compounds belonging to several classes. From M. vitis-idaea, eight methylenequinone celastroids (1-8) were isolated, four of which (4-7) were hitherto unknown. Additionally, from M. spinosa, two known celastroids, a known celastroid dimer (9), three pentacyclic triterpenoids (10-12) and six ß-dihydroagarofuran sesquiterpenoid alkaloids (13-18) were identified. Compounds 4-7 were active against six solid tumor cell lines at micromolar concentrations.
Assuntos
Alcaloides/isolamento & purificação , Alcaloides/farmacologia , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Maytenus/química , Plantas Medicinais/química , Sesquiterpenos/isolamento & purificação , Sesquiterpenos/farmacologia , Triterpenos/isolamento & purificação , Triterpenos/farmacologia , Alcaloides/química , Antineoplásicos Fitogênicos/química , Argentina , Relação Dose-Resposta a Droga , Ensaios de Seleção de Medicamentos Antitumorais , Células HeLa , Humanos , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Raízes de Plantas/química , Sesquiterpenos/química , Triterpenos/químicaRESUMO
This paper reports the in vitro antifungal activity of propolis extracts from the province of Tucuman (Argentina) as well as the identification of their main antifungal compounds and botanical origin. The antifungal activity was determined by the microdilution technique, using reference microorganisms and clinical isolates. All dermatophytes and yeasts tested were strongly inhibited by different propolis extracts (MICs between 16 and 125 microg mL(-1)). The most susceptible species were Microsporum gypseum, Trichophyton mentagrophytes, and Trichophyton rubrum. The main bioactive compounds were 2',4'-dihydroxy-3'-methoxychalcone 2 and 2',4'-dihydroxychalcone 3. Both displayed strong activity against clinical isolates of T. rubrum and T. mentagrophytes (MICs and MFCs between 1.9 and 2.9 microg mL(-1)). Additionally, galangin 5, pinocembrin 6, and 7-hydroxy-8-methoxyflavanone 9 were isolated from propolis samples and Zuccagnia punctata exudates, showing moderate antifungal activity. This is the first study matching the chemical profile of Z. punctata Cav. exudates with their corresponding propolis, giving strong evidence on the botanical origin of the studied propolis.
Assuntos
Antifúngicos/farmacologia , Fabaceae/química , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Própole/química , Própole/farmacologia , Argentina , Arthrodermataceae/efeitos dos fármacos , Leveduras/efeitos dos fármacosRESUMO
The MeOH extract of aerial parts of Flourensia riparia Grisebach (Asteraceae) afforded a sesquiterpene lactone, 4beta-hydroxy-4,10alpha-dimethyl-7alphaH,8alphaH-eudesman-11-ene-8,12-olide, together with septuplinolide, its isomer at positions C-5 and C-10. In addition, known flavonoids, p-hydroxyacetophenone derivatives, carabrone and isoalantolactone were identified. Three known flavonoids and a benzofuran were isolated from Flourensia campestris Wedd.